標題: Titlebook: Bacteriophages; Methods and Protocol Martha R. J. Clokie,Andrew Kropinski,Rob Lavigne Book 2019 Springer Science+Business Media, LLC, part [打印本頁] 作者: CROSS 時間: 2025-3-21 17:33
書目名稱Bacteriophages影響因子(影響力)
書目名稱Bacteriophages影響因子(影響力)學(xué)科排名
書目名稱Bacteriophages網(wǎng)絡(luò)公開度
書目名稱Bacteriophages網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Bacteriophages被引頻次
書目名稱Bacteriophages被引頻次學(xué)科排名
書目名稱Bacteriophages年度引用
書目名稱Bacteriophages年度引用學(xué)科排名
書目名稱Bacteriophages讀者反饋
書目名稱Bacteriophages讀者反饋學(xué)科排名
作者: Seizure 時間: 2025-3-21 21:40
Introduction of Phage Genome into , by Electroporation,oving medical treatments. Here we describe its use for introduction of phage genomic DNA into . cells, including preparation of electrocompetent cells, electric pulse optimization and recovery of electrotransformed cells. The technique can also be adapted for other bacterial species.作者: 宿醉 時間: 2025-3-22 03:26 作者: 有偏見 時間: 2025-3-22 06:08 作者: biopsy 時間: 2025-3-22 10:43
Springer Science+Business Media, LLC, part of Springer Nature 2019作者: jovial 時間: 2025-3-22 14:25 作者: 脊椎動物 時間: 2025-3-22 18:54 作者: 內(nèi)行 時間: 2025-3-23 00:13
Max-Emanuel Geis,Daniel Krausnickoving medical treatments. Here we describe its use for introduction of phage genomic DNA into . cells, including preparation of electrocompetent cells, electric pulse optimization and recovery of electrotransformed cells. The technique can also be adapted for other bacterial species.作者: Free-Radical 時間: 2025-3-23 02:59 作者: 自傳 時間: 2025-3-23 07:39 作者: indemnify 時間: 2025-3-23 13:38
Jürgen Baumert,Hans-Peter Füsselof revealing the metabolic state of mycobacterial cells and therefore their response to antibiotics. Here we described a simple and rapid method for drug susceptibility testing (DST) of . using a fluorescence microscope, a flow cytometer, or a fluorimeter in a convenient multiwell format.作者: 特別容易碎 時間: 2025-3-23 16:55
Jürgen Baumert,Hans-Peter Füssel a narrow subset of diagnostic settings. More recently, however, advances in DNA sequencing and the introduction of more sensitive reporter systems have enabled novel engineering methods, which in turn have broadened the scope of modern phage diagnostics. Here we describe advanced methods to enginee作者: mucous-membrane 時間: 2025-3-23 18:36
Max-Emanuel Geis,Daniel Krausnickoving medical treatments. Here we describe its use for introduction of phage genomic DNA into . cells, including preparation of electrocompetent cells, electric pulse optimization and recovery of electrotransformed cells. The technique can also be adapted for other bacterial species.作者: aneurysm 時間: 2025-3-24 00:02
Max-Emanuel Geis,Daniel Krausnickrgeted gene is cloned in a . shuttle vector. Using an in vitro enzymatic procedure dependent on mutant oligonucleotide primers, a mutation is inserted into the cloned gene, replacing an early lysine codon (AAA or AAG) with a nonsense codon (TAG or TAA). The mutant plasmid is recovered by transformat作者: 運動性 時間: 2025-3-24 04:27 作者: parsimony 時間: 2025-3-24 09:05
Max-Emanuel Geis,Daniel Krausnicklation method. Here, we demonstrate that T4-like phages can be efficiently isolated by affinity chromatography. This approach employs specific affinity tags (GST (glutathione S-transferase) or His-tag) that allow for the isolation of the phage. These affinity tags are exposed on the phage head using作者: Irrepressible 時間: 2025-3-24 11:54
Jürgen Baumert,Hans-Peter Füssel of bacterial pathogens. Whole phage can carry reporter genes to alter the phenotype of the target pathogen. Phage can also act as staining agents or the progeny of the infection process can be detected. Alternatively, using phage components as probes offer advantages over whole phage particles, inc作者: 有法律效應(yīng) 時間: 2025-3-24 18:50
https://doi.org/10.1007/978-3-642-15523-9sualization of enzymatically active protein species separated by molecular mass. The strategy is to perform SDS-PAGE on the proteins in question while including an opaque substrate of the enzyme embedded within the polyacrylamide gel. Here, we describe a zymogram protocol for phage lytic proteins (p作者: Commonplace 時間: 2025-3-24 20:30 作者: falsehood 時間: 2025-3-25 03:11 作者: OATH 時間: 2025-3-25 03:19
§88 Grundrechtsschutz im f?deralen Europaluence the host metabolism to facilitate the infection process. Some of these proteins strongly perturb the host cell, ultimately leading to cell death. These growth-inhibitory phage proteins presumably target key metabolic processes, which may provide a basis for innovative phage-derived antibacter作者: photophobia 時間: 2025-3-25 10:15 作者: geriatrician 時間: 2025-3-25 13:58 作者: cardiovascular 時間: 2025-3-25 19:35 作者: deactivate 時間: 2025-3-25 22:09 作者: 全國性 時間: 2025-3-26 04:12 作者: CORD 時間: 2025-3-26 08:22 作者: 外科醫(yī)生 時間: 2025-3-26 12:01
Martha R. J. Clokie,Andrew Kropinski,Rob LavigneIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: Communicate 時間: 2025-3-26 14:13 作者: 秘密會議 時間: 2025-3-26 17:36 作者: 捏造 時間: 2025-3-26 23:52 作者: 男生如果明白 時間: 2025-3-27 02:08
Duckweed (,) and Alfalfa (,) as Bacterial Infection Model Systems,ults faster, are more convenient, and less expensive than many animal infection models. Here, we describe the use of two different plant-based infection models for the discovery of virulence genes and factors involved in bacterial pathogenesis.作者: Induction 時間: 2025-3-27 07:49 作者: 使困惑 時間: 2025-3-27 12:39 作者: 出價 時間: 2025-3-27 15:23 作者: Spirometry 時間: 2025-3-27 21:48
Charles B. Blankart,Erik R. Fastennd the discrimination between infection stages. This book chapter covers all aspects of the procedure, from phage probe design and synthesis, to the?phageFISH protocol itself, to microscopy and image analysis.作者: Organonitrile 時間: 2025-3-27 22:07
Jürgen Baumert,Hans-Peter Füsselve enabled novel engineering methods, which in turn have broadened the scope of modern phage diagnostics. Here we describe advanced methods to engineer the genomes of bacteriophages in a modular and rapid fashion.作者: 截斷 時間: 2025-3-28 06:08 作者: 擁擠前 時間: 2025-3-28 08:53
§35 Direkte Demokratie und F?deralismuse describe methods to characterize phage–biofilm interactions, specifically to assess biofilm biomass and to visualize the biofilm structure, discriminating infected cells using targeted molecular probes.作者: Cursory 時間: 2025-3-28 10:41
https://doi.org/10.1007/978-3-642-16883-3on into society and its further development. Recently, silkworm larvae have been recognized as highly convenient and useful model animals, and an alternative to higher animals. We describe the procedure for experimental phage therapy to treat . infections in silkworm larvae.作者: 類型 時間: 2025-3-28 17:29
https://doi.org/10.1007/978-3-658-23230-6e available. The embryonated chicken egg model has been used to study the virulence of many pathogens. We describe here a procedure to test the efficacy of phage therapy to treat colibacillosis using a chicken embryo lethality assay, this being potentially applied to others bacterial infection.作者: 幾何學(xué)家 時間: 2025-3-28 20:32 作者: 原告 時間: 2025-3-29 02:39 作者: 閑蕩 時間: 2025-3-29 06:41
Engineering Bacteriophage-Based Biosensors,ve enabled novel engineering methods, which in turn have broadened the scope of modern phage diagnostics. Here we describe advanced methods to engineer the genomes of bacteriophages in a modular and rapid fashion.作者: 鑲嵌細工 時間: 2025-3-29 09:04 作者: 嗎啡 時間: 2025-3-29 14:30 作者: vocation 時間: 2025-3-29 19:04
Use of a Silkworm Larva Model in Phage Therapy Experiments,on into society and its further development. Recently, silkworm larvae have been recognized as highly convenient and useful model animals, and an alternative to higher animals. We describe the procedure for experimental phage therapy to treat . infections in silkworm larvae.作者: AROMA 時間: 2025-3-29 21:40 作者: 有效 時間: 2025-3-30 00:49 作者: objection 時間: 2025-3-30 06:13
Book 2019nt of phage therapy by outlining novel models. The chapters in this book cover molecular topics such as PhageFISH for monitoring phage infections at single cell level; the analysis of phage-host protein-protein interactions using Strep-tag? II purifications; and also application driven chapters incl作者: 甜瓜 時間: 2025-3-30 12:11
Jürgen Baumert,Hans-Peter Füssel to orient the anchoring is desired in order to optimize binding efficiency. This chapter presents various methods that have been employed for the attachment of phage and phage components onto support structures such as beads, filters, and sensor surfaces.作者: fidelity 時間: 2025-3-30 14:32 作者: membrane 時間: 2025-3-30 18:08
Book 2019, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Innovative and thorough, .Bacteriophages: Methods and Protocols, Volume IV. is a valuable resource for both establishedand novice phage scientists..作者: Aggrandize 時間: 2025-3-31 00:25 作者: vocation 時間: 2025-3-31 03:21
https://doi.org/10.1007/978-3-658-23230-6the systemic infection model using the fruit fly, .. Since phages, unlike antibacterial chemicals, can be easily and sensitively enumerated by simple assays, it is also possible to address the pharmacokinetic properties of administered phages even in this small-scale infection model.作者: Commentary 時間: 2025-3-31 06:10 作者: 一小塊 時間: 2025-3-31 13:09
https://doi.org/10.1007/978-3-642-15523-9ptible bacterium have embedded in the SDS gel matrix is performed. After electrophoresis, the SDS is removed from the zymogram gel, allowing the proteins (now separated by molecular mass) to assume an active conformation and ultimately digest the opaque substrate (yielding a nonopaque product). This作者: 發(fā)起 時間: 2025-3-31 15:04 作者: 胖人手藝好 時間: 2025-3-31 19:16 作者: Project 時間: 2025-3-31 22:14
Peptidoglycan Hydrolytic Activity of Bacteriophage Lytic Proteins in Zymogram Analysis,ptible bacterium have embedded in the SDS gel matrix is performed. After electrophoresis, the SDS is removed from the zymogram gel, allowing the proteins (now separated by molecular mass) to assume an active conformation and ultimately digest the opaque substrate (yielding a nonopaque product). This作者: connoisseur 時間: 2025-4-1 02:15
,Analyzing Phage–Host Protein–Protein Interactions Using ,-tag, II Purifications, purification of the tagged protein which allows the copurification of all bacterial and phage specific interacting proteins. After SDS-PAGE analysis and an in-gel trypsin digestion, the purified interacting proteins are identified by mass spectrometry analysis. The identification of phage proteins 作者: catagen 時間: 2025-4-1 08:16
Screening for Growth-Inhibitory ORFans in ,-Infecting Bacteriophages,-inducible promoter. Furthermore, we describe a method to examine the effect of the phage proteins in different hosts, using different vector copy numbers. Finally, we explain how to investigate the effect of ORFan expression on the host morphology using time-lapse microscopy.作者: BUDGE 時間: 2025-4-1 13:32 作者: 憤怒事實 時間: 2025-4-1 15:06 作者: Intercept 時間: 2025-4-1 20:22
Fluoromycobacteriophages for Drug Susceptibility Testing (DST) of Mycobacteria,of revealing the metabolic state of mycobacterial cells and therefore their response to antibiotics. Here we described a simple and rapid method for drug susceptibility testing (DST) of . using a fluorescence microscope, a flow cytometer, or a fluorimeter in a convenient multiwell format.作者: 注視 時間: 2025-4-2 01:32
Engineering Bacteriophage-Based Biosensors, a narrow subset of diagnostic settings. More recently, however, advances in DNA sequencing and the introduction of more sensitive reporter systems have enabled novel engineering methods, which in turn have broadened the scope of modern phage diagnostics. Here we describe advanced methods to enginee作者: 蛤肉 時間: 2025-4-2 04:53 作者: 機警 時間: 2025-4-2 07:58
Site-Specific Mutagenesis of , Phage SPO1,rgeted gene is cloned in a . shuttle vector. Using an in vitro enzymatic procedure dependent on mutant oligonucleotide primers, a mutation is inserted into the cloned gene, replacing an early lysine codon (AAA or AAG) with a nonsense codon (TAG or TAA). The mutant plasmid is recovered by transformat
