標題: Titlebook: Bacterial and Archaeal Motility; Tohru Minamino,Makoto Miyata,Keiichi Namba Book 2023 The Editor(s) (if applicable) and The Author(s), und [打印本頁] 作者: 空格 時間: 2025-3-21 17:07
書目名稱Bacterial and Archaeal Motility影響因子(影響力)
書目名稱Bacterial and Archaeal Motility影響因子(影響力)學科排名
書目名稱Bacterial and Archaeal Motility網(wǎng)絡(luò)公開度
書目名稱Bacterial and Archaeal Motility網(wǎng)絡(luò)公開度學科排名
書目名稱Bacterial and Archaeal Motility被引頻次
書目名稱Bacterial and Archaeal Motility被引頻次學科排名
書目名稱Bacterial and Archaeal Motility年度引用
書目名稱Bacterial and Archaeal Motility年度引用學科排名
書目名稱Bacterial and Archaeal Motility讀者反饋
書目名稱Bacterial and Archaeal Motility讀者反饋學科排名
作者: A保存的 時間: 2025-3-22 00:15 作者: Motilin 時間: 2025-3-22 03:20
Site-Specific Isotope Labeling of FliG for Studying Structural Dynamics Using Nuclear Magnetic Reson conducted structural dynamics analyses using solution nuclear magnetic resonance (NMR) to elucidate the detailed functions of flagellar motor proteins. Here, we introduce the analysis of the FliG protein, which is a flagellar motor protein, focusing on the preparation method of the original stable isotope-labeled protein.作者: bleach 時間: 2025-3-22 05:41
Kapitel 8 Kommunikationsgrundrechteembrane export gate complex, which acts as a proton/protein antiporter that couples proton flow with flagellar protein export, and a cytoplasmic ATPase ring complex, which works as an activator of the export gate complex. Three transmembrane proteins, FliP, FliQ, and FliR, form a core structure of t作者: 誘拐 時間: 2025-3-22 10:17
https://doi.org/10.1007/978-3-662-68581-5in export apparatus, a member of the type III secretion system (T3SS). To reveal the molecular mechanism of protein transport by the T3SS, accurate measurement of protein transport under various conditions is essential. In this chapter, we describe an in vitro method for flagellar protein transport 作者: 隨意 時間: 2025-3-22 16:41 作者: 是突襲 時間: 2025-3-22 19:51
https://doi.org/10.1007/978-3-662-68581-5ller, a short hook as a universal joint, and a basal body as a rotary motor. The filament is made up of more than 20,000 flagellin molecules and can grow to several micrometers long but only 20 nanometers thick. The regulation of flagellar assembly and ejection is important for bacterial environment作者: 無聊點好 時間: 2025-3-22 23:29
Kapitel 8 Kommunikationsgrundrechter, the hook as a universal joint, and the basal body as a rotary motor. In the case of ., the filament length is 10–15?μm, which is more than ten times longer than the size of the cell. The filament is composed of only one component protein, flagellin, and is made of 11 protofilaments. The filament 作者: 變量 時間: 2025-3-23 03:12 作者: 開花期女 時間: 2025-3-23 08:42
Selektive staatliche Begünstigunginflux (typically H. or Na.) through an ion channel of the stator. Ion influx induces conformational changes in the stator, followed by changes in the interactions between the stator and rotor. The driving force to rotate the flagellum is thought to be generated by changing the stator-rotor interact作者: obtuse 時間: 2025-3-23 10:38 作者: SNEER 時間: 2025-3-23 13:59
Selektive staatliche Begünstigungs PomA and PomB, is responsible for energy conversion in the motor. To understand the coupling of the Na. flux with torque generation, it is essential to clearly identify the Na.-binding sites and the Na. flux pathway through the stator channel. Although residues essential for Na. flux have been ide作者: Critical 時間: 2025-3-23 18:08 作者: 保留 時間: 2025-3-23 22:22
Allgemeiner Rahmen des Vergaberechts bacterium. The flagellar motor comprises a rotor and a dozen stator units, converting ion flux through the stator unit into torque. However, the energy coupling mechanism has not been fully understood. Various methods for rotation measurement have been developed to understand the rotation mechanism作者: AWL 時間: 2025-3-24 03:39 作者: 最初 時間: 2025-3-24 09:18 作者: 調(diào)整校對 時間: 2025-3-24 11:28 作者: 座右銘 時間: 2025-3-24 16:10
Allgemeiner Rahmen des Vergaberechtsforce can be estimated from the swimming velocity and the drag coefficient based on the assumption that the swimming force balances with the drag force exerted on the bacterium. Though the velocity-force relation provides a significant clue to understand the swimming mechanism, the odd configuration作者: 種屬關(guān)系 時間: 2025-3-24 22:06 作者: Bombast 時間: 2025-3-25 01:46 作者: Infiltrate 時間: 2025-3-25 03:57
Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/180333.jpg作者: 輕彈 時間: 2025-3-25 10:18 作者: Diatribe 時間: 2025-3-25 14:17
978-1-0716-3062-4The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines作者: BLANK 時間: 2025-3-25 16:59 作者: VOK 時間: 2025-3-25 22:54
https://doi.org/10.1007/978-3-662-68581-5asurement of protein transport under various conditions is essential. In this chapter, we describe an in vitro method for flagellar protein transport assay using inverted membrane vesicles (IMVs) prepared from . cells. This method can easily and precisely control the condition around the T3SS and be applied to other T3SSs.作者: 整理 時間: 2025-3-26 04:00 作者: 姑姑在炫耀 時間: 2025-3-26 08:01
Selektive staatliche Begünstigungscence imaging by highly inclined and laminated optical sheet (HILO) microscopy are presented in this chapter. Furthermore, we introduce methods for pH imaging in magnetosome lumen as an application of fluorescence magnetosome imaging.作者: Terminal 時間: 2025-3-26 12:24
Allgemeiner Rahmen des Vergaberechtsamphibious motility is believed to be significant for pathogenicity. This chapter focuses on the zoonotic spirochete . and describes the method for measuring the spirochete adhesion and surface motility.作者: Offset 時間: 2025-3-26 12:48 作者: 細胞 時間: 2025-3-26 19:44
Purification of Na+-Driven MotPS Stator Complexes and Single-Molecule Imaging by High-Speed Atomic F mechanism and motor assembly. High-speed atomic force microscopy (HS-AFM) is a powerful tool for directly observing dynamically acting biological molecules with high spatiotemporal resolution without interfering with their function. Here, we describe protocols for single-molecule imaging of the Na.-driven MotPS stator complex by HS-AFM.作者: FER 時間: 2025-3-26 22:56 作者: 抗體 時間: 2025-3-27 02:53
Analysis of Adhesion and Surface Motility of a Spirochete Bacteriumamphibious motility is believed to be significant for pathogenicity. This chapter focuses on the zoonotic spirochete . and describes the method for measuring the spirochete adhesion and surface motility.作者: 意外 時間: 2025-3-27 08:36
Purification of the Transmembrane Polypeptide Channel Complex of the , Flagellar Type III Secretion he export gate complex, and this core complex serves as a polypeptide channel that allows flagellar structural subunits to be translocated across the cytoplasmic membrane. Here, we describe the methods for overproduction, solubilization, and purification of the . FliP/FliQ/FliR complex.作者: 希望 時間: 2025-3-27 12:44 作者: 誓言 時間: 2025-3-27 14:41 作者: Sinus-Rhythm 時間: 2025-3-27 21:07
Swarming Motility Assays in daptive resistance to antibiotics. Described here are protocols to cultivate, verify, and study swimming and swarming motility in ., and an additional “border-crossing” assay, where cells “primed” to swarm are presented with an environmental challenge such as antibiotics to assess their propensity to handle the challenge.作者: 拖債 時間: 2025-3-27 22:02
Force Measurement of Bacterial Swimming Using Optical Tweezers of bacteria could develop problems with the accuracy of the force estimation. This chapter describes the force measurement using optical tweezers. The method uses parameters obtained from the shape and movement of a microsphere attached to the bacteria, improving the quantitativeness of force measurement.作者: accordance 時間: 2025-3-28 04:31 作者: Affection 時間: 2025-3-28 08:24 作者: hangdog 時間: 2025-3-28 13:16 作者: animated 時間: 2025-3-28 16:16 作者: 沒收 時間: 2025-3-28 18:47
Measurements of the Ion Channel Activity of the Transmembrane Stator Complex in the Bacterial Flagelmbrane stator complex over-expressed in . cells using fluorescent indicators. Intensity measurements of fluorescent indicators using either a fluorescence spectrophotometer or microscope allow quantitative detection of changes in the intracellular ion concentrations due to the ion channel activity of the transmembrane protein complex.作者: 水獺 時間: 2025-3-29 02:42
Purification of the Na+-Driven PomAB Stator Complex and Its Analysis Using ATR-FTIR Spectroscopyhe binding of Na. to purified PomAB stator complex using attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy. This method demonstrates that Na.-binding sites are formed by critical aspartic acid and threonine residues located in the transmembrane segments of PomAB.作者: lanugo 時間: 2025-3-29 06:16 作者: Culmination 時間: 2025-3-29 10:22 作者: Lipoprotein(A) 時間: 2025-3-29 14:55 作者: 人類 時間: 2025-3-29 16:45 作者: Cytology 時間: 2025-3-29 23:42 作者: 窩轉(zhuǎn)脊椎動物 時間: 2025-3-30 00:37 作者: Adrenaline 時間: 2025-3-30 06:55
Allgemeiner Rahmen des Vergaberechtsa wide range of external load, but the elasticity of the axial parts of the flagellum, such as the hook and filament, limits the spatiotemporal resolution. In this chapter, we describe a bead assay optimized for the analysis of the flagellar motor dynamics at near zero load.作者: Parallel 時間: 2025-3-30 10:04
High-Resolution Rotation Assay of the Bacterial Flagellar Motor Near Zero Loads Using a Mutant Havina wide range of external load, but the elasticity of the axial parts of the flagellum, such as the hook and filament, limits the spatiotemporal resolution. In this chapter, we describe a bead assay optimized for the analysis of the flagellar motor dynamics at near zero load.作者: grounded 時間: 2025-3-30 15:49
Book 2023ective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.?.Authoritative and thorough, .Bacterialand Archaeal Motility. is the ideal reference for researchers working in this vital area of microbiology..作者: Fibroid 時間: 2025-3-30 18:04
Purification of the Transmembrane Polypeptide Channel Complex of the , Flagellar Type III Secretion embrane export gate complex, which acts as a proton/protein antiporter that couples proton flow with flagellar protein export, and a cytoplasmic ATPase ring complex, which works as an activator of the export gate complex. Three transmembrane proteins, FliP, FliQ, and FliR, form a core structure of t作者: Ceramic 時間: 2025-3-31 00:30 作者: Genteel 時間: 2025-3-31 03:54 作者: 揭穿真相 時間: 2025-3-31 05:23 作者: hypnotic 時間: 2025-3-31 12:45
