作者: laceration 時(shí)間: 2025-3-21 20:45 作者: febrile 時(shí)間: 2025-3-22 01:27 作者: accomplishment 時(shí)間: 2025-3-22 06:35
Host-Pathogen Transcriptomics by Dual RNA-Seqyotic cells under a given condition. However, traditional approaches were unsuitable to measure the abundance of transcripts across kingdoms, which is relevant for biological processes such as bacterial infections of mammalian host cells. This changed with the establishment of “Dual RNA-seq,” which 作者: 減去 時(shí)間: 2025-3-22 09:29
Identification of New Bacterial Small RNA Targets Using MS2 Affinity Purification Coupled to RNA Seqher promote their degradation or interfere with translation initiation. Because a single sRNA can regulate a considerable number of target mRNAs, we seek to identify those targets rapidly and reliably. Here, we present a robust method based on the co-purification of target mRNAs bound to MS2-tagged 作者: Acquired 時(shí)間: 2025-3-22 16:44
Assessment of External Guide Sequences’ (EGS) Efficiency as Inducers of RNase P-Mediated Cleavage ofd as the enzyme that mediates cleavage of precursor tRNAs at the 5′-end termini to generate the mature tRNAs. An important characteristic of RNase P is that its specificity depends on the structure rather than the sequence of the RNA substrate. Any RNA species that interacts with an antisense molecu作者: 課程 時(shí)間: 2025-3-22 17:22 作者: Constitution 時(shí)間: 2025-3-22 21:41 作者: 神經(jīng) 時(shí)間: 2025-3-23 02:38 作者: 使混合 時(shí)間: 2025-3-23 09:16 作者: microscopic 時(shí)間: 2025-3-23 12:46
An Integrated Cell-Free Assay to Study Translation Regulation by Small Bacterial Noncoding RNAsm, motility, and pathogenesis. Using the bacterial carbon storage regulator/regulator of secondary metabolism (Csr/Rsm) system, we here describe an .-based cell-free translation assay that allows a quantitative analysis of translation regulation by ncRNAs and their corresponding translation represso作者: colony 時(shí)間: 2025-3-23 17:22
Quantitative Super-Resolution Imaging of Small RNAs in Bacterial Cellson (smFISH), super-resolved single-fluorophore microscopy, and clustering analysis. Compared to smFISH imaging with diffraction-limited fluorescence microscopy, our method provides better quantification for short and abundant RNA (such as sRNAs) in a small volume of bacterial cells. Our method can a作者: 刺激 時(shí)間: 2025-3-23 21:22 作者: 得罪人 時(shí)間: 2025-3-24 01:22 作者: callous 時(shí)間: 2025-3-24 03:37
High-Resolution, High-Throughput Analysis of Hfq-Binding Sites Using UV Crosslinking and Analysis ofcteria. They play key roles in most aspects of bacterial physiology, including central metabolism, nutrient acquisition, virulence, biofilm formation, and outer membrane composition. RNA sequencing technologies have accelerated the identification of bacterial regulatory RNAs and are now being employ作者: Noisome 時(shí)間: 2025-3-24 07:47
Identification of New Bacterial Small RNA Targets Using MS2 Affinity Purification Coupled to RNA SeqsRNAs expressed in vivo. After purification of the tagged-sRNA, we use RNAseq to determine the identity of all RNA interacting partners and their enrichment level. We describe how to analyze the RNAseq data through the Galaxy Project Platform bioinformatics tools to identify new mRNA targets. This technique is applicable to most sRNAs of . and ..作者: 珊瑚 時(shí)間: 2025-3-24 13:53
Mapping Changes in Cell Surface Protein Expression Through Selective Labeling of Live Cellsthod comprises a direct labeling of surface proteins on living cells using fluorescent dyes, followed by total protein extraction and subsequent separation of these extracts by 2D gel electrophoresis.作者: 無(wú)底 時(shí)間: 2025-3-24 15:04 作者: APNEA 時(shí)間: 2025-3-24 21:18 作者: 動(dòng)機(jī) 時(shí)間: 2025-3-25 02:21
Absolute Regulatory Small Noncoding RNA Concentration and Decay Rates Measurements in ,us our analyses on the . Gram-negative bacterium as a model. The information described in this chapter provides a guideline to help develop a protocol in order to assess these important parameters and to identify RNA-processing enzymes involved in sRNA degradation processes.作者: 規(guī)范要多 時(shí)間: 2025-3-25 04:46 作者: ANTI 時(shí)間: 2025-3-25 10:54
https://doi.org/10.1007/978-3-322-90621-2thod comprises a direct labeling of surface proteins on living cells using fluorescent dyes, followed by total protein extraction and subsequent separation of these extracts by 2D gel electrophoresis.作者: ANIM 時(shí)間: 2025-3-25 12:19
https://doi.org/10.1007/978-3-662-67086-6e describe the design of a mutational strategy used to analyze the base pairing between two CU-rich regions of the sRNA Rli22 and the AG-rich Shine-Dalgarno region of the mRNA . in .. The protocol can be employed for mutational studies of base pairing between any sRNA and its mRNA target(s).作者: 喊叫 時(shí)間: 2025-3-25 19:50
https://doi.org/10.1007/978-3-658-41781-9thod of ultrapure OMVs and the subsequent extraction of RNA and basic steps of RNA-Seq analysis. Bacterial culture, extracellular supernatant concentration, OMV purification, and the subsequent RNA extraction out of OMVs are described. Specific pitfalls within the protocol and RNA contamination sources are highlighted.作者: Hyperlipidemia 時(shí)間: 2025-3-25 21:24 作者: 洞穴 時(shí)間: 2025-3-26 04:12
Book 2018ips on troubleshooting and avoiding known pitfalls...Authoritative and cutting-edge, .Bacterial Regulatory RNA: Methods and Protocols .serves as a guidebook for scientists working toward the development of new tools and proceduresfor the vital field of sRNA biology..作者: bypass 時(shí)間: 2025-3-26 06:56
Book 2018in sections covering different aspects of the biology of that field: identification of ncRNAs, their differential expression, characterization of their structure, abundance, intracellular location and function, their interaction with RNA binding proteins, and plausible applications of ncRNA elements作者: Ingredient 時(shí)間: 2025-3-26 10:05
1064-3745 ation advice from the experts.This volume details the most important methods used for studying prokaryotic non-coding RNAs and their protein accomplices. Chapters present methods in sections covering different aspects of the biology of that field: identification of ncRNAs, their differential express作者: 慢慢啃 時(shí)間: 2025-3-26 15:09 作者: 角斗士 時(shí)間: 2025-3-26 19:37 作者: anthropologist 時(shí)間: 2025-3-26 21:53 作者: 打折 時(shí)間: 2025-3-27 04:45
Digital Legacy in der Palliativversorgungicroscopy, our method provides better quantification for short and abundant RNA (such as sRNAs) in a small volume of bacterial cells. Our method can also be directly used for the quantification of messenger RNAs (mRNAs).作者: 口訣法 時(shí)間: 2025-3-27 07:35 作者: 聲明 時(shí)間: 2025-3-27 09:38 作者: Spinal-Tap 時(shí)間: 2025-3-27 13:52
Fluorescence-Based Methods for Characterizing RNA Interactions In Vivohe regulatory interactions RNAs perform in vivo: (i) the in vivo RNA Structural Sensing System (iRS.) for measuring RNA accessibility and (ii) the trifluorescence complementation (TriFC) assay for measuring RNA-protein interactions.作者: ALT 時(shí)間: 2025-3-27 21:40 作者: Facet-Joints 時(shí)間: 2025-3-27 23:42
https://doi.org/10.1007/978-3-662-67086-6f ncRNA and repressor protein. We demonstrate our protocol with a comparative translation activation analysis of the RsmX, RsmY, and RsmZ sRNAs from ., which reveals a superior efficacy of RsmZ over RsmX and RsmY.作者: Finasteride 時(shí)間: 2025-3-28 04:30
An Integrated Cell-Free Assay to Study Translation Regulation by Small Bacterial Noncoding RNAsf ncRNA and repressor protein. We demonstrate our protocol with a comparative translation activation analysis of the RsmX, RsmY, and RsmZ sRNAs from ., which reveals a superior efficacy of RsmZ over RsmX and RsmY.作者: WAG 時(shí)間: 2025-3-28 07:55
https://doi.org/10.1007/978-1-4939-7634-8sRNA; intracellular localization; prokaryotic non-coding RNAs; ncRNAs; RNA binding proteins作者: harangue 時(shí)間: 2025-3-28 12:59 作者: anthropologist 時(shí)間: 2025-3-28 16:48 作者: Yag-Capsulotomy 時(shí)間: 2025-3-28 21:31
Dietmar Pinkernell,Michael Bargende numbers of sRNA candidates. This chapter outlines a potential workflow for computational sRNA analyses and describes in detail methods for homolog detection, target prediction, and functional characterization based on enrichment analysis. The cyanobacterial sRNA IsaR1 is used as a specific example.作者: Diverticulitis 時(shí)間: 2025-3-29 02:35 作者: FLEET 時(shí)間: 2025-3-29 06:15 作者: 不安 時(shí)間: 2025-3-29 10:08 作者: BOOST 時(shí)間: 2025-3-29 12:19
Motortechnische Grundlagen des Dieselmotorsher promote their degradation or interfere with translation initiation. Because a single sRNA can regulate a considerable number of target mRNAs, we seek to identify those targets rapidly and reliably. Here, we present a robust method based on the co-purification of target mRNAs bound to MS2-tagged 作者: hermetic 時(shí)間: 2025-3-29 19:24 作者: 不給啤 時(shí)間: 2025-3-29 23:10
Direkteinblasung bei Dieselmotorengulate gene expression at the transcriptional level. In this case, the sRNA-dependent machinery modulates the activity of the transcription termination factor Rho, a ring-shaped RNA translocase/helicase that dissociates transcription elongation complexes at specific loci of the bacterial genome. Her作者: harbinger 時(shí)間: 2025-3-30 01:20 作者: ARK 時(shí)間: 2025-3-30 04:34
Instandhaltung von Dieselmotoren,ic physiological behavior of bacterial RNAs. Here we document the step-by-step design and application of two fluorescence-based methods for studying the regulatory interactions RNAs perform in vivo: (i) the in vivo RNA Structural Sensing System (iRS.) for measuring RNA accessibility and (ii) the tri作者: DOLT 時(shí)間: 2025-3-30 09:52
https://doi.org/10.1007/978-3-662-67086-6ted by electrophoretic mobility shift assay. In this assay, regions engaged in base pairing are analyzed by introducing mutations in one of the RNAs that prevent sRNA–mRNA complex formation, followed by the introduction of complementary mutations in its partner RNA that restore base pairing. Here, w作者: jealousy 時(shí)間: 2025-3-30 12:41
https://doi.org/10.1007/978-3-662-67086-6m, motility, and pathogenesis. Using the bacterial carbon storage regulator/regulator of secondary metabolism (Csr/Rsm) system, we here describe an .-based cell-free translation assay that allows a quantitative analysis of translation regulation by ncRNAs and their corresponding translation represso作者: mosque 時(shí)間: 2025-3-30 18:20 作者: 為現(xiàn)場(chǎng) 時(shí)間: 2025-3-30 20:53
https://doi.org/10.1007/978-3-658-41781-9nents, such as proteins, peptidoglycans, lipopolysaccharides, DNA, and RNA. To examine if OMV-associated RNA molecules are bacterial degradation products and/or are functionally active, it is necessary to extract RNA from pure OMVs for subsequent analysis. Therefore, we describe here an isolation me作者: 勛章 時(shí)間: 2025-3-31 04:08 作者: 糾纏 時(shí)間: 2025-3-31 07:07 作者: GROVE 時(shí)間: 2025-3-31 13:16 作者: disciplined 時(shí)間: 2025-3-31 16:59
Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/180319.jpg
