作者: 泥土謙卑 時(shí)間: 2025-3-21 23:31 作者: 有花 時(shí)間: 2025-3-22 01:18
Grundlagen der Dienstleistungsproduktion unique sequences called spacers. CRISPRs together with a set of genes called . for CRISPR associated, constitute a defence mechanism against invasion by foreign sequences. We describe protocols and bioinformatics tools that allow the identification of CRISPRs, their comparison and their component d作者: STRIA 時(shí)間: 2025-3-22 05:22
Grundlagen des Dienstleistungsmarketing,ements of molecular and computational biology to identify small noncoding RNA (sRNA) transcripts. We focus on the key features of this approach, which include size-fractionation of input RNA, direct detection of array hybridization with antibodies that recognize RNA:DNA hybrids, and correlation-base作者: COLON 時(shí)間: 2025-3-22 10:21 作者: Eeg332 時(shí)間: 2025-3-22 16:26
Handbuch Dienstleistungsmarketing with the loss of a gene. As such, they are invaluable tools for assigning gene function in the post-genomic era. In this protocol, we describe methodologies for creating and employing barcoded deletion mutants in competitive screens as well as how to analyze the ensuing results.作者: grieve 時(shí)間: 2025-3-22 17:25
https://doi.org/10.1007/978-3-662-07711-5usands of nucleotides and simultaneously estimate the size of an RNA and detect its degradation/processing products. The method does not rely on enzymes such as reverse transcriptases or RNA ligases used in most advanced RNA detection methods, which can be advantageous since biases in detection of i作者: 助記 時(shí)間: 2025-3-23 00:15 作者: 心胸狹窄 時(shí)間: 2025-3-23 02:46
Anlagen zur Start- und Zündhilfentribution discusses the methods available for predicting RNA structure. Secondary structure is the set of the canonical base pairs, and secondary structure can be accurately determined by comparative sequence analysis. Secondary structure can also be predicted. The most commonly used method is free作者: endocardium 時(shí)間: 2025-3-23 06:52
Anlagen zur Start- und Zündhilfermation on structural organizations of key elements of cellular machinery. However, crystallization of RNA–protein complexes is often challenging and requires special approaches. Here we review the purification of RNA, RNA-binding proteins, and the formation and crystallization of RNA–protein comple作者: 冥界三河 時(shí)間: 2025-3-23 12:43 作者: Cardioversion 時(shí)間: 2025-3-23 16:03
überwachung, Wartung und Diagnoseears there has been a rapid expansion in the discovery and characterization of sRNAs in a diverse range of bacteria. Paradigm shifts in our understanding of the breadth of posttranscriptional control by sRNAs were achieved in a number of pioneering studies that involved immunoprecipitation of a know作者: Allowance 時(shí)間: 2025-3-23 18:40 作者: Priapism 時(shí)間: 2025-3-23 23:38
Geschichte und Grundlagen des DieselmotorsRNA residues to enzymatic digestion gives information about the RNA secondary structure, the location of protein binding sites, and the effects of protein binding on the RNA structure. Here we present a detailed protocol for using RNase T2, which cleaves single stranded RNA with a preference for A n作者: CRAFT 時(shí)間: 2025-3-24 06:04
Abgasemission von Dieselmotorenicts the targets of a sRNA by identifying messages with significant potential to base pair with the sRNA. Since base pairing potential alone is insufficient to accurately identify sRNA targets, . integrates several additional features of RNA interactions when predicting regulatory targets of a sRNA.作者: follicle 時(shí)間: 2025-3-24 08:45 作者: insincerity 時(shí)間: 2025-3-24 11:11 作者: Conclave 時(shí)間: 2025-3-24 16:11 作者: 使糾纏 時(shí)間: 2025-3-24 19:13
Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/180318.jpg作者: Kernel 時(shí)間: 2025-3-25 02:32 作者: 暗諷 時(shí)間: 2025-3-25 03:58
978-1-4939-5941-9Springer Science+Business Media, LLC 2012作者: frugal 時(shí)間: 2025-3-25 07:56 作者: 蕁麻 時(shí)間: 2025-3-25 14:23 作者: 恭維 時(shí)間: 2025-3-25 18:57
Bioinformatic Discovery of Bacterial Regulatory RNAs Using SIPHT searching for regions of intergenic sequence conservation upstream of predicted intrinsic transcription terminators. Each locus is then annotated for numerous features that provide clues about its potential function and/or enable the most reliable candidates to be identified.作者: Onerous 時(shí)間: 2025-3-25 23:24
Crystallization of RNA–Protein Complexes: From Synthesis and Purification of Individual Components tin vitro, and proteins that were overexpressed in . and purified to be RNase-free. The complex was crystallized using a sitting drop setup; initial screening for suitable crystallization conditions was performed using a sparse matrix approach.作者: 山頂可休息 時(shí)間: 2025-3-26 01:31 作者: sclera 時(shí)間: 2025-3-26 07:27
Anlagen zur Start- und Zündhilfe microscopy. This chapter describes the use of FISH to visualize tmRNA, a regulatory RNA required for .-translation. The method can be adapted to visualize the localization of other regulatory and messenger RNAs as well.作者: 蹣跚 時(shí)間: 2025-3-26 10:57
Anlagen zur Start- und Zündhilfeg in terms of stoichiometry, affinity, and heat (enthalpy), while DSC can provide RNA stability in terms of heat capacity, melting temperature, and folding enthalpy. Here, we offer detailed experimental protocols for studying such RNA systems with commercially available conventional and high-throughput ITC and DSC instruments.作者: 熱心助人 時(shí)間: 2025-3-26 16:27
https://doi.org/10.1007/978-3-662-07709-2ative polyacrylamide gels in Tris/borate/EDTA buffer, although an alternative Tris-glycine buffering system is superior in many situations. Here, we describe both gel shift methods, along with strategies to improve separation of protein–RNA complexes from free RNA, which can be a particular challenge for small RNA binding proteins.作者: 口音在加重 時(shí)間: 2025-3-26 18:56
Geschichte und Grundlagen des Dieselmotorsucleotides, to footprint the protein Hfq on the . mRNA leader. This protocol covers how to form the RNP complex, determine the correct dose of enzyme, footprint the protein, and analyze the cleavage pattern using primer extension.作者: 背信 時(shí)間: 2025-3-27 00:58 作者: collagenase 時(shí)間: 2025-3-27 03:54
Grundlagen der Dienstleistungsproduktion by foreign sequences. We describe protocols and bioinformatics tools that allow the identification of CRISPRs, their comparison and their component determination (the direct repeats and the spacers). A schematic representation of the spacer organization can be produced, allowing an easy comparison between strains.作者: 挖掘 時(shí)間: 2025-3-27 07:49
Grundlagen des Dienstleistungsmarketing, include size-fractionation of input RNA, direct detection of array hybridization with antibodies that recognize RNA:DNA hybrids, and correlation-based computational methods for automated sRNA identification and boundary determination.作者: Angioplasty 時(shí)間: 2025-3-27 09:40
Abgasemission von Dieselmotorenicient to accurately identify sRNA targets, . integrates several additional features of RNA interactions when predicting regulatory targets of a sRNA. In this chapter, we provide a detailed guide on how to use . to identify targets of sRNA regulation.作者: white-matter 時(shí)間: 2025-3-27 14:04 作者: indenture 時(shí)間: 2025-3-27 18:48
A Strategy for Identifying Noncoding RNAs Using Whole-Genome Tiling Arrays include size-fractionation of input RNA, direct detection of array hybridization with antibodies that recognize RNA:DNA hybrids, and correlation-based computational methods for automated sRNA identification and boundary determination.作者: 圓桶 時(shí)間: 2025-3-27 23:45 作者: CHOKE 時(shí)間: 2025-3-28 05:54
Anlagen zur Start- und Zündhilfed in a set of homologous sequences. Additionally, tertiary structure, the three-dimensional arrangement of atoms, can be modeled with guidance from comparative analysis and experimental techniques. New approaches are also available for predicting tertiary structure.作者: Mendicant 時(shí)間: 2025-3-28 10:05 作者: OATH 時(shí)間: 2025-3-28 12:47
Book 2012t of bacterial physiology. In .Bacteria Regulatory RNA: Methods and Protocols, .expert researchers in the field detail many of the methods which are now commonly used to study bacterial regulatory RNA. These include methods and techniques to identify regulatory RNAs, characterizing the function and 作者: 豐滿中國(guó) 時(shí)間: 2025-3-28 16:19
RNA Visualization in Bacteria by Fluorescence In Situ Hybridization microscopy. This chapter describes the use of FISH to visualize tmRNA, a regulatory RNA required for .-translation. The method can be adapted to visualize the localization of other regulatory and messenger RNAs as well.作者: 郊外 時(shí)間: 2025-3-28 21:09 作者: Parallel 時(shí)間: 2025-3-29 00:25
Gel Mobility Shift Assays to Detect Protein–RNA Interactionsative polyacrylamide gels in Tris/borate/EDTA buffer, although an alternative Tris-glycine buffering system is superior in many situations. Here, we describe both gel shift methods, along with strategies to improve separation of protein–RNA complexes from free RNA, which can be a particular challenge for small RNA binding proteins.作者: 阻塞 時(shí)間: 2025-3-29 04:23
RNase Footprinting of Protein Binding Sites on an mRNA Target of Small RNAsucleotides, to footprint the protein Hfq on the . mRNA leader. This protocol covers how to form the RNP complex, determine the correct dose of enzyme, footprint the protein, and analyze the cleavage pattern using primer extension.作者: 盲信者 時(shí)間: 2025-3-29 11:15 作者: Gene408 時(shí)間: 2025-3-29 13:15 作者: 執(zhí) 時(shí)間: 2025-3-29 15:49 作者: exclusice 時(shí)間: 2025-3-29 23:10 作者: BARGE 時(shí)間: 2025-3-30 01:33
Use of Semi-quantitative Northern Blot Analysis to Determine Relative Quantities of Bacterial CRISPR stabilities of both pre-crRNA and crRNA. The procedures described in this chapter can be used with very minor modifications to monitor the abundance and stabilities of transcripts of various lengths from many bacterial sources.作者: Scleroderma 時(shí)間: 2025-3-30 06:48 作者: 冒失 時(shí)間: 2025-3-30 11:38
1064-3745 troubleshooting and avoiding known pitfalls..?.?Authoritative and practical, .Bacteria Regulatory RNA: Methods and Protocols. seeks to aid scientists in the further study of bacterial regulatory RNA. .978-1-4939-5941-9978-1-61779-949-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 路標(biāo) 時(shí)間: 2025-3-30 12:44 作者: lipoatrophy 時(shí)間: 2025-3-30 18:11 作者: 轉(zhuǎn)折點(diǎn) 時(shí)間: 2025-3-30 21:49 作者: 男生戴手銬 時(shí)間: 2025-3-31 02:56
Genetic Screens to Identify Bacterial sRNA Regulatorsthways in bacteria. While finding the targets of a given sRNA has been the focus of many studies, fewer methods have been described to uncover which, if any, sRNAs regulate a given gene. Here I present two genetic screens that are designed to search for sRNAs regulating a gene of interest. Before th作者: 繁忙 時(shí)間: 2025-3-31 07:52
