作者: Emasculate 時間: 2025-3-21 22:48
CRISPR Interference-Based Functional Small RNA Genomicsation of a guide library targeting the full intergenic sRNA repertoire of this organism and its application to identify sRNA knockdown-associated fitness effects. Our protocol is generic and thus suitable for the systematic assessment of sRNA-associated phenotypes in a wide range of bacterial specie作者: 漸強 時間: 2025-3-22 04:19
Analysis of sRNAs and Their mRNA Targets in ,: Focus on Half-Life Determinationize RNA. Here we discuss our experience in using alternative RNA purification methods for analysis of sRNA and mRNA of .. Additionally, we show that other translational inhibitors (besides tetracycline) also cause attenuation giving rise to the rnTrpL sRNA. Furthermore, we discuss the importance of 作者: ADAGE 時間: 2025-3-22 05:14 作者: Mystic 時間: 2025-3-22 11:11
In-Gel Cyanoethylation for Pseudouridines Mass Spectrometry Detection of Bacterial Regulatory RNAency,?the?protocol was applied to bacterial regulatory RNAs from .: 6S RNA and transfer-messenger RNA (tmRNA, also known as 10Sa RNA). Moreover, ribonuclease P (RNase P)?was also mapped using this approach. This method enabled the detection of several ψ at single nucleotide resolution.作者: Nefarious 時間: 2025-3-22 13:25 作者: 嗎啡 時間: 2025-3-22 18:56 作者: armistice 時間: 2025-3-22 23:22
https://doi.org/10.1007/978-3-7091-7994-9ation of a guide library targeting the full intergenic sRNA repertoire of this organism and its application to identify sRNA knockdown-associated fitness effects. Our protocol is generic and thus suitable for the systematic assessment of sRNA-associated phenotypes in a wide range of bacterial specie作者: HALL 時間: 2025-3-23 04:39 作者: Synthesize 時間: 2025-3-23 07:53 作者: 松軟無力 時間: 2025-3-23 10:58 作者: 水槽 時間: 2025-3-23 17:43
1064-3745 ation advice from the experts.This second edition details new and updated methods used for studying prokaryotic non-coding RNAs and their protein accomplices.?Chapters detail discovery of ncRNAs, characterization of their structure, functions, and their interactomes.?Written in the highly successful作者: ANA 時間: 2025-3-23 19:21
Handbuch Datenschutz und IT-Sicherheitolate vesicles in order to understand their biological functions. Here we describe a polymer-based precipitation method allowing high-yield isolation of extracellular vesicles and their cargo RNA from the Gram-positive bacterium ..作者: 十字架 時間: 2025-3-24 00:55
Handbuch Demokratische Republik Kongo previous studies, the sRNA predictions are accurate and reliable. In this chapter, we outline the sRNA detection algorithm, important parameters used, step-by-step execution, and data interpretation with a . study as an example. Following those procedures, novel sRNA can be revealed by ANNOgesic.作者: outer-ear 時間: 2025-3-24 02:40 作者: 內(nèi)行 時間: 2025-3-24 08:28 作者: 鑲嵌細(xì)工 時間: 2025-3-24 13:12 作者: 高原 時間: 2025-3-24 18:23 作者: 認(rèn)為 時間: 2025-3-24 22:43
Experimental Validation of RNA–RNA Interactions by Electrophoretic Mobility Shift Assaycomplex formation. Next, base pairing is restored by introducing complementary mutations in its partner RNA. Here, we describe the mutational strategy and experimental methods used to validate specific base pairing between two RNA species.作者: VEST 時間: 2025-3-25 02:21
Directed Screening for sRNA Targets in , Using a Plasmid LibraryA signaling is identifying the sRNA regulators of specific genes. Here, we describe a protocol for making an sRNA expression library and using this library to screen for sRNA regulators of genes of interest in .. This library can be easily expanded and adapted to use in other bacteria.作者: ALIEN 時間: 2025-3-25 06:40 作者: JOG 時間: 2025-3-25 09:50 作者: 冬眠 時間: 2025-3-25 14:38
Book 2024Latest editionovery of ncRNAs, characterization of their structure, functions, and their interactomes.?Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reprod作者: 靦腆 時間: 2025-3-25 18:21
Analysis of Phage Regulatory RNAs: Sequencing Library Construction from the Fraction of Small Prokarules of this size from bacterial cells. To meet these difficulties, we describe here the few-step procedure of isolation, purification, selection, and sequencing library preparation that is dedicated to the fraction of very small, bacterial RNA molecules.作者: Ligneous 時間: 2025-3-25 22:28
Dynamics and Function of sRNA/mRNAs Under the Scrutiny of Computational Simulation Methods, in general, and the peculiarities of RNA simulations, in particular. We discuss the strengths and limitations of the technique and provide examples of its application to elucidate small RNA’s performance.作者: 很像弓] 時間: 2025-3-26 03:50 作者: dura-mater 時間: 2025-3-26 07:18
Belgisch-Kongo und Unabh?ngigkeital species. Here we describe methods for the extraction and purification of vesicles produced by Gram-negative bacteria, such as . and ., and address methods for the extraction of OMVs-derived sRNA and techniques for the analysis of sRNAs.作者: 鞭打 時間: 2025-3-26 10:42
Extraction and Purification of Outer Membrane Vesicles and Their Associated RNAsal species. Here we describe methods for the extraction and purification of vesicles produced by Gram-negative bacteria, such as . and ., and address methods for the extraction of OMVs-derived sRNA and techniques for the analysis of sRNAs.作者: Euphonious 時間: 2025-3-26 15:25 作者: 冥想后 時間: 2025-3-26 19:26
,Wasserstoffüberspannung und Katalyse,he localization of minimal regulatory regions of an sRNA, the determination of the secondary structures of both interacting RNAs and their complex as well as the analysis of RNA chaperones that may promote the sRNA/mRNA interaction.作者: Urea508 時間: 2025-3-27 00:36 作者: Jacket 時間: 2025-3-27 04:33 作者: 下船 時間: 2025-3-27 07:23
In Vitro Methods for the Investigation of sRNA-mRNA Interactions in he localization of minimal regulatory regions of an sRNA, the determination of the secondary structures of both interacting RNAs and their complex as well as the analysis of RNA chaperones that may promote the sRNA/mRNA interaction.作者: Arctic 時間: 2025-3-27 12:30 作者: Exaggerate 時間: 2025-3-27 15:40 作者: Obsequious 時間: 2025-3-27 19:34
Extraction and Purification of Outer Membrane Vesicles and Their Associated RNAsia with the environment. OMVs act as information carriers and encapsulate various molecules such as proteins, lipids, metabolites, and RNAs. OMVs and sRNAs play a broad range of functions from pathogenesis to stress resistance, to biofilm formation and both mediate interkingdom signaling. Various st作者: Pelago 時間: 2025-3-28 01:46
Analysis of Phage Regulatory RNAs: Sequencing Library Construction from the Fraction of Small Prokaried. The first microRNA-size functional ribonucleic acid occurring in a bacterial cell has been described only recently, and it was found to be encoded by a bacteriophage. One of the reasons for such a scarcity in this field is the lack of procedures intended for the isolation and selection of molec作者: 武器 時間: 2025-3-28 03:33 作者: POLYP 時間: 2025-3-28 06:36
Ribosome Profiling Methods Adapted to the Study of RNA-Dependent Translation Regulation in rious stresses encountered in their host environments. . is a well-studied model for RNA-mediated regulation of virulence and pathogenicity, with sRNAs playing significant roles in shaping . interactions with human and animal hosts. By modulating the translation and/or stability of target mRNAs, sRN作者: Badger 時間: 2025-3-28 13:30
CRISPR Interference-Based Functional Small RNA Genomicsns a major bottleneck. Gene inactivation through random transposon insertion has proven extremely valuable in discovering hidden gene functions. However, this approach is biased toward long genes and usually results in the underrepresentation of sRNA mutants. In contrast, CRISPR interference (CRISPR作者: incredulity 時間: 2025-3-28 15:22
Investigation of sRNA-mRNA Interactions in , In Vivoher established or significantly improved in our group and successfully employed to characterize a number of sRNA/target mRNA systems in .. Whereas in Chap. 8, we describe a combination of in vitro methods, e.g., EMSA and RNA secondary structure probing, we focus here on the investigation of RNA-RNA作者: Irritate 時間: 2025-3-28 19:06
In Vitro Methods for the Investigation of sRNA-mRNA Interactions in 1, 2). Here, we describe in vitro methods for the analysis of sRNA/mRNA interactions. All these methods have been either elaborated or significantly improved in our group and successfully applied to characterize a number of sRNA/target mRNA systems in . for which we provide examples from our own wo作者: 禮節(jié) 時間: 2025-3-29 00:13 作者: 扔掉掐死你 時間: 2025-3-29 04:12 作者: aneurysm 時間: 2025-3-29 07:28 作者: LUMEN 時間: 2025-3-29 11:26 作者: 勛章 時間: 2025-3-29 16:05 作者: enumaerate 時間: 2025-3-29 21:34
Evaluation of 5′-End Phosphorylation for Small RNA Stability and Target Regulation In Vivoary transcripts, undergo dephosphorylation or originate via processing. Often, 5′ groups hallmark RNAs for rapid decay, but whether this also applies to sRNAs is little explored. Moreover, the sRNA 5′P group could activate endoribonuclease RNase E to cleave the base-paired target RNA, but a tool for作者: AMPLE 時間: 2025-3-30 02:11 作者: 察覺 時間: 2025-3-30 05:44
Directed Screening for sRNA Targets in , Using a Plasmid Libraryility. This posttranscriptional regulation has been shown to be essential and critical for bacterial physiology. One of the challenges of studying sRNA signaling is identifying the sRNA regulators of specific genes. Here, we describe a protocol for making an sRNA expression library and using this li作者: 黃瓜 時間: 2025-3-30 10:38
Bacterial Regulatory RNA978-1-0716-3565-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: PARA 時間: 2025-3-30 12:53 作者: atrophy 時間: 2025-3-30 17:19
