標(biāo)題: Titlebook: Bacterial Protein Secretion Systems; Methods and Protocol Laure Journet,Eric Cascales Book 2017 Springer Science+Business Media LLC 2017 Fr [打印本頁(yè)] 作者: 烤問(wèn) 時(shí)間: 2025-3-21 17:55
書目名稱Bacterial Protein Secretion Systems影響因子(影響力)
書目名稱Bacterial Protein Secretion Systems影響因子(影響力)學(xué)科排名
書目名稱Bacterial Protein Secretion Systems網(wǎng)絡(luò)公開度
書目名稱Bacterial Protein Secretion Systems網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Bacterial Protein Secretion Systems被引頻次
書目名稱Bacterial Protein Secretion Systems被引頻次學(xué)科排名
書目名稱Bacterial Protein Secretion Systems年度引用
書目名稱Bacterial Protein Secretion Systems年度引用學(xué)科排名
書目名稱Bacterial Protein Secretion Systems讀者反饋
書目名稱Bacterial Protein Secretion Systems讀者反饋學(xué)科排名
作者: 胎兒 時(shí)間: 2025-3-21 23:47 作者: Parabola 時(shí)間: 2025-3-22 03:33 作者: 商品 時(shí)間: 2025-3-22 04:51
https://doi.org/10.1007/978-1-4939-7033-9Fractionation; Lipoproteins; Peptidoglycan; Protease; sub-cellular localisation作者: ILEUM 時(shí)間: 2025-3-22 12:30 作者: 藥物 時(shí)間: 2025-3-22 13:08 作者: 倔強(qiáng)一點(diǎn) 時(shí)間: 2025-3-22 21:00
Birger P. Priddat,Christoph Meinekemethods exist for predicting surface-exposed lipoproteins, and therefore lipoprotein topology must be experimentally tested. This chapter describes three distinct but complementary methods for the detection of surface-exposed proteins: cell surface protein labeling, accessibility to extracellular protease and antibodies.作者: 保全 時(shí)間: 2025-3-22 23:28 作者: 教育學(xué) 時(shí)間: 2025-3-23 05:26
D. Michael Albrecht,Armin T?pferuped into three fundamentally different approaches: signal-based, global-property-based and homology-based prediction. In this chapter, the strengths and drawbacks of each of these approaches is described through many examples of methods that predict secretion, integration into membranes, or subcell作者: 兵團(tuán) 時(shí)間: 2025-3-23 06:00 作者: NAUT 時(shí)間: 2025-3-23 11:08
Birger P. Priddat,Christoph Meinekerocesses of protein export/secretion. In this chapter we describe a method to determine their precise localisation, for example inner versus outer membrane, in Gram-negative bacteria using human opportunistic pathogen . as a model. A fusion protein between a given putative lipoprotein and the red fl作者: 彩色 時(shí)間: 2025-3-23 17:31
Birger P. Priddat,Christoph Meineke cytoplasmic membrane. Three enzymatic steps are involved in the synthesis of mature triacylated lipoprotein: prolipoprotein converts into diacylglyceryl-prolipoprotein that in turn converts into apolipoprotein, which is finally converted into mature triacylated lipoprotein. Here we describe the det作者: THROB 時(shí)間: 2025-3-23 18:39
Birger P. Priddat,Christoph Meinekesic techniques in cell biology can be applied to characterise bacterial membranes and their membrane protein constituents. Here we describe a protocol for the purification of outer and inner membranes from .. The procedure can be applied with minor modifications to other bacterial species, including作者: choleretic 時(shí)間: 2025-3-23 23:58 作者: 一窩小鳥 時(shí)間: 2025-3-24 04:43
Finanzcontrolling in der Unternehmenspraxisnteractions with membrane protein partners or participate in energetic processes. The number, location, and orientation of these helices is referred to as .. Bitopic membrane proteins that consist of a single membrane-embedded domain connecting two soluble domains are distinguished from polytopic on作者: Instantaneous 時(shí)間: 2025-3-24 09:36 作者: 要素 時(shí)間: 2025-3-24 12:55 作者: Malcontent 時(shí)間: 2025-3-24 15:56
Controllership in der Unternehmenspraxisl envelopes contain a rigid netlike peptidoglycan structure that protects cells from osmotic lysis. Trans-envelope systems thus must interact with the peptidoglycan barrier to generate gaps or anchor structures to the peptidoglycan scaffold. Here we describe methods to use in vivo cross-linking and 作者: puzzle 時(shí)間: 2025-3-24 22:02 作者: 前奏曲 時(shí)間: 2025-3-25 00:32
Preiscontrolling in der Unternehmenspraxisracterizing protein–protein interactions in vivo. This system is based on the interaction-mediated reconstitution of a cyclic adenosine monophosphate (cAMP) signaling cascade in .. As BACTH uses a diffusible cAMP messenger molecule, the physical association between the two interacting chimeric prote作者: 想象 時(shí)間: 2025-3-25 06:35 作者: 誘拐 時(shí)間: 2025-3-25 09:48
Finanzcontrolling in der Unternehmenspraxisblishing which component and substrate interactions are direct or indirect further facilitates (1) advancing the architecture and assembly of the machines and (2) understanding the substrates’ translocation mechanistics. Currently, though biochemical means exist for identifying such direct interacti作者: SPER 時(shí)間: 2025-3-25 13:20
Wolfgang Becker,Patrick Ulrich,Bj?rn Baltzerght also be mediated by helix–helix contacts in the inner membrane. Here we describe genetic assays commonly used to test interactions between transmembrane α-helices in their native membrane environment. These assays are based on the reconstitution of dimeric regulators allowing the control of expr作者: 詞匯 時(shí)間: 2025-3-25 19:21
Finanzcontrolling in der Unternehmenspraxisactions are crucial in many biological processes in living cells. Genetic (such as yeast two-hybrid, Y2H) and biochemical (such as co-immunoprecipitation, co-IP) methods are the methods commonly used at the beginning of a study to identify the interacting proteins. Immunoprecipitation (IP), a method作者: 變量 時(shí)間: 2025-3-25 22:22 作者: Lipoprotein 時(shí)間: 2025-3-26 00:38
Handbuch Controlling der Kommunikationmains of their client proteins, forming sequential intermediate complexes. The short-lived nature of these interactions poses a big challenge in the identification of the key factors involved in transport reactions and their mechanism of action. Site-directed photocrosslinking is a powerful method f作者: 用肘 時(shí)間: 2025-3-26 05:19 作者: LAY 時(shí)間: 2025-3-26 10:44 作者: amenity 時(shí)間: 2025-3-26 14:37
Cell Fractionation,he different compartments of . by a fractionation method and to determine the presence of the protein of interest. For inner membrane proteins we propose a method to discriminate between integral and peripheral membrane proteins.作者: 步兵 時(shí)間: 2025-3-26 20:15
,Protein–Protein Interactions: Yeast Two-Hybrid System,into proximity, thereby activating the transcription of reporter genes. This technology can be widely used to identify interacting partners, confirm suspected interactions, and define interacting domains.作者: STING 時(shí)間: 2025-3-26 23:05
Finanzcontrolling in der Unternehmenspraxises that consist of multiple membrane-spanning helices connected by extramembrane domains. Defining inner membrane protein topology could be achieved by different methods. Here we describe a protease accessibility assay that makes it possible to define topology based on digestion profiles.作者: Exhilarate 時(shí)間: 2025-3-27 03:24
Controllership in der Unternehmenspraxis putative extracellular or intracellular loops of a membrane protein are replaced one at a time by cysteine residue, and the orientation with respect to the membrane is evaluated using a pair of membrane-impermeable nondetectable and detectable thiol-reactive labeling reagents.作者: LINES 時(shí)間: 2025-3-27 08:07 作者: 聚集 時(shí)間: 2025-3-27 11:20
Finanzcontrolling in der Unternehmenspraxisons, they primarily remain in vitro and are quite labor intensive. Thus, adopting genetic approaches to help visualize these interactions in vivo is quick and advantageous. Here I describe bimolecular fluorescence complementation and cytology-based two-hybrid assays that could easily be adopted to understand the bacterial secretions systems.作者: isotope 時(shí)間: 2025-3-27 17:02 作者: Cirrhosis 時(shí)間: 2025-3-27 18:24
Probing Inner Membrane Protein Topology by Proteolysis,es that consist of multiple membrane-spanning helices connected by extramembrane domains. Defining inner membrane protein topology could be achieved by different methods. Here we describe a protease accessibility assay that makes it possible to define topology based on digestion profiles.作者: 征兵 時(shí)間: 2025-3-27 23:54 作者: 儀式 時(shí)間: 2025-3-28 04:00 作者: 諷刺滑稽戲劇 時(shí)間: 2025-3-28 08:12
,Protein–Protein Interactions: Cytology Two-Hybrid,ons, they primarily remain in vitro and are quite labor intensive. Thus, adopting genetic approaches to help visualize these interactions in vivo is quick and advantageous. Here I describe bimolecular fluorescence complementation and cytology-based two-hybrid assays that could easily be adopted to understand the bacterial secretions systems.作者: 佛刊 時(shí)間: 2025-3-28 12:07 作者: blithe 時(shí)間: 2025-3-28 15:33
1064-3745 ation advice from the experts.This volume details protocols that cover the broad arsenal of techniques used to study a secretion system from A to Z. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifyin作者: 刺激 時(shí)間: 2025-3-28 20:08 作者: 細(xì)絲 時(shí)間: 2025-3-29 01:17
Birger P. Priddat,Christoph Meinekeryl-prolipoprotein that in turn converts into apolipoprotein, which is finally converted into mature triacylated lipoprotein. Here we describe the detection of one of these intermediate forms of lipoprotein, diacylglyceryl-prolipoprotein, using .H-palmitate labeling and inhibition by globomycin and detection by fluorography.作者: 領(lǐng)帶 時(shí)間: 2025-3-29 03:46 作者: 關(guān)節(jié)炎 時(shí)間: 2025-3-29 08:21
https://doi.org/10.1007/978-3-658-26431-4 of the peptidoglycan. Their activities are spatially controlled to avoid cell lysis and to create localized rearrangement of the cell wall. This is assured by interaction with the structural subunits of the apparatus. Here we describe protocols to test the peptidoglycan hydrolase activity of these proteins in vitro and in solution.作者: 譏諷 時(shí)間: 2025-3-29 12:19 作者: HAVOC 時(shí)間: 2025-3-29 19:01 作者: 竊喜 時(shí)間: 2025-3-29 23:16 作者: Leisureliness 時(shí)間: 2025-3-30 00:56
Defining Membrane Protein Localization by Isopycnic Density Gradients, for the purification of outer and inner membranes from .. The procedure can be applied with minor modifications to other bacterial species, including those carrying capsular polysaccharide attached to the outer membrane.作者: Aggressive 時(shí)間: 2025-3-30 07:07 作者: Intellectual 時(shí)間: 2025-3-30 11:48 作者: 比賽用背帶 時(shí)間: 2025-3-30 13:00 作者: Alcove 時(shí)間: 2025-3-30 17:06 作者: 圍裙 時(shí)間: 2025-3-30 21:58 作者: BIAS 時(shí)間: 2025-3-31 00:57
,Protein–Protein Interactions: Co-Immunoprecipitation,eraction events in vivo. Co-IP experiments can identify proteins via direct or indirect interactions or in a protein complex. Here, we use . type VI secretion system (T6SS) sheath components TssB-TssC. interaction as an example to describe the principle, procedure, and experimental problems of co-IP.作者: CAMEO 時(shí)間: 2025-3-31 05:22
Book 2017s on troubleshooting and avoiding known pitfalls..Authoritative and cutting-edge, .Bacterial Protein Secretion Systems: Methods and Protocol .aims to provide techniques that are not restricted to the study of secretion systems but arealso of specific interest for any researcher interested on multi-protein complexes of the bacterial cell envelope..作者: eardrum 時(shí)間: 2025-3-31 10:16
Book 2017and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or secretion, identifying secreted effectors 作者: 印第安人 時(shí)間: 2025-3-31 16:13 作者: occurrence 時(shí)間: 2025-3-31 20:46
Identification of Protein Secretion Systems in Bacterial Genomes Using MacSyFinder,t checks whether the genetic content and organization of clusters satisfy the constraints of the model. TXSScan models can be customized to search for variants of known systems. The models can also be built from scratch to identify novel systems. In this chapter, we describe a complete pipeline of a作者: 解決 時(shí)間: 2025-3-31 23:11 作者: CRP743 時(shí)間: 2025-4-1 05:32 作者: 旋轉(zhuǎn)一周 時(shí)間: 2025-4-1 06:47
,Protein–Protein Interaction: Tandem Affinity Purification in Bacteria,ion based on the binding of ProtA to IgG coated beads, TEV protease cleavage releases CBP-tagged bait-protein along with its partners for a second round of purification on calmodulin affinity resin and leaves behind protein contaminants bound to IgG. Creating the TAP-tag translational fusion at the 作者: BUST 時(shí)間: 2025-4-1 12:17 作者: outer-ear 時(shí)間: 2025-4-1 18:20 作者: 領(lǐng)導(dǎo)權(quán) 時(shí)間: 2025-4-1 22:22 作者: 低三下四之人 時(shí)間: 2025-4-2 00:20
Handbuch Controlling der Kommunikationion based on the binding of ProtA to IgG coated beads, TEV protease cleavage releases CBP-tagged bait-protein along with its partners for a second round of purification on calmodulin affinity resin and leaves behind protein contaminants bound to IgG. Creating the TAP-tag translational fusion at the 作者: licence 時(shí)間: 2025-4-2 04:14
Identification of Protein Secretion Systems in Bacterial Genomes Using MacSyFinder, barriers. They have evolved by co-option of components from other envelope-associated cellular machineries, making them sometimes difficult to identify and discriminate. Here, we describe how to identify protein secretion systems in bacterial genomes using MacSyFinder. This flexible computational t作者: Preamble 時(shí)間: 2025-4-2 07:01
Protein Sorting Prediction,uped into three fundamentally different approaches: signal-based, global-property-based and homology-based prediction. In this chapter, the strengths and drawbacks of each of these approaches is described through many examples of methods that predict secretion, integration into membranes, or subcell作者: Osmosis 時(shí)間: 2025-4-2 11:14
Cell Fractionation,nt compartments: the cytoplasm, the inner membrane, the periplasm, the outer membrane and the extracellular medium. Different approaches can be used to determine the protein localisation within a cell such as in silico identification of protein signal sequences and motifs, electron microscopy and im
