標題: Titlebook: Bacterial Polysaccharides; Methods and Protocol Inka Brockhausen Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 20 [打印本頁] 作者: Lactase 時間: 2025-3-21 19:29
書目名稱Bacterial Polysaccharides影響因子(影響力)
書目名稱Bacterial Polysaccharides影響因子(影響力)學科排名
書目名稱Bacterial Polysaccharides網絡公開度
書目名稱Bacterial Polysaccharides網絡公開度學科排名
書目名稱Bacterial Polysaccharides被引頻次
書目名稱Bacterial Polysaccharides被引頻次學科排名
書目名稱Bacterial Polysaccharides年度引用
書目名稱Bacterial Polysaccharides年度引用學科排名
書目名稱Bacterial Polysaccharides讀者反饋
書目名稱Bacterial Polysaccharides讀者反饋學科排名
作者: 招募 時間: 2025-3-22 00:11 作者: EXULT 時間: 2025-3-22 00:23
Beendigung von Gesch?ftsbeziehungen-(2-acetamido-2-deoxy-3-O-β-.-galactopyranosyl-α-.-galactopyranosyl) diphosphate [.-Gal(β1-3)-.-GalNAcα-PP-PhU, .]. We describe the procedures for identification and structure estimation of compounds by TLC, NMR, and MS. We also include the biochemical testing of . with α2,3-sialyltransferase WbwA f作者: 加入 時間: 2025-3-22 06:36 作者: 闡釋 時間: 2025-3-22 12:27
Customizable Cloning of Whole Polysaccharide Gene Clusters by Yeast Homologous Recombination,ranged, allowing the function and/or specificity of individual genes to be examined. Since the vector can be easily modified to include other bacterial replicons, it can also be broadly applied to the functional analysis of a wide range of bacterial gene clusters and operons.作者: sinoatrial-node 時間: 2025-3-22 15:42 作者: Invertebrate 時間: 2025-3-22 19:26
Sulfhydryl Labeling as a Tool to Investigate the Topology of Membrane Proteins Involved in Lipopolyious places in the protein under in vivo and in vitro conditions. Cysteine residues are detected with polyethylene glycol-maleimide (PEG-Mal). This procedure can be applied to crude bacterial cell extracts and does not require protein purification.作者: 狗舍 時間: 2025-3-22 22:44 作者: Enliven 時間: 2025-3-23 04:39
Purification of Capsular Polysaccharide Complex from Gram-Negative Bacteria,cterium . serving as an example. The method efficiently removes all detectable endotoxins and other lipid components, proteins, and nucleic acids, providing a source of capsular polysaccharide for immunologic study.作者: 個人長篇演說 時間: 2025-3-23 08:49
Utilization of Fluorescently Tagged Synthetic Acceptor Molecules for In Vitro Characterization of azation of enzyme activity. Here we describe an application of this technique which was previously used to functionally characterize a dual-domain glycosyltransferase enzyme, KpsC, involved in capsular polysaccharide biosynthesis in ..作者: exercise 時間: 2025-3-23 11:43 作者: Intact 時間: 2025-3-23 16:15 作者: 淘氣 時間: 2025-3-23 21:36 作者: Focus-Words 時間: 2025-3-24 01:11 作者: 喪失 時間: 2025-3-24 06:05 作者: GRAIN 時間: 2025-3-24 09:00
https://doi.org/10.1007/978-3-531-92639-1eptors followed by detection of .-nitrophenolate. GT reactions coupled with phosphatases and detection of inorganic phosphate are suitable for most GTs. These assays permit convenient quantification of GT activities and kinetics without the use of radioactive sugars.作者: SOB 時間: 2025-3-24 12:10
Extraction and Purification of Wall-Bound Polymers of Gram-Positive Bacteria,ical characterization. This protocol describes the hydrofluoric acid extraction and purification of the secondary cell wall polymer of . that is also found in the envelope of the other members of the . sensu lato group of bacteria.作者: 發(fā)怨言 時間: 2025-3-24 17:35
Glycosyltransferase Activity Assay Using Colorimetric Methods,eptors followed by detection of .-nitrophenolate. GT reactions coupled with phosphatases and detection of inorganic phosphate are suitable for most GTs. These assays permit convenient quantification of GT activities and kinetics without the use of radioactive sugars.作者: SMART 時間: 2025-3-24 21:34
Book 2019ures and functions of bacterial polysaccharides, their attachments to proteins and lipids, their role in biofilm formation, as well as their biosynthesis. With the emerging bacterial resistance to commonly used antibiotics world-wide, these techniques to study the outer polysaccharides of bacteria, 作者: corpus-callosum 時間: 2025-3-24 23:36 作者: 并置 時間: 2025-3-25 06:32
,Haupttragwerke der überbauten,nvironment. Here, we describe a simple method for purifying CWPS by extraction of bacterial cells with consecutive acid treatments. Purified CWPS are obtained by gel-filtration chromatography following treatment with HF. We also provide the methodology to easily assess the integrity of CWPS using high-resolution magic-angle spinning (HR-MAS) NMR.作者: 擁護 時間: 2025-3-25 08:16
Die Wirkung des Bundesverfassungsgerichtsate squarate derivative and the product glycoconjugate can be easily purified using centrifugal filtration devices. This method is experimentally simple and affords glycoconjugates with predictable carbohydrate–protein ratio (carbohydrate content), high conjugation efficiency, and excellent yield.作者: ALE 時間: 2025-3-25 13:06 作者: 撫慰 時間: 2025-3-25 19:45 作者: 表狀態(tài) 時間: 2025-3-25 21:08 作者: 過于平凡 時間: 2025-3-26 00:55 作者: 記成螞蟻 時間: 2025-3-26 05:37
Designing Glycosyltransferase Expression Constructs for Improved Purification, Protein Yield, and Cn simplified by making a subconstruct that is better behaved in solution, and perhaps monofunctional. In this chapter we review bioinformatics tools and strategies that can be used for designing such constructs of glycosyltransferases.作者: 使服水土 時間: 2025-3-26 12:12 作者: hedonic 時間: 2025-3-26 13:27
Manfred Curbach,Gerhard Mehlhorn structural characterization can be performed by MALDI-TOF mass spectrometry. Precise quantification of PIMs, LM, and LAM can be performed by quantification of glycan staining using analytical software. The?metabolic radiolabeling protocol is also described.作者: eczema 時間: 2025-3-26 16:46
Besonderheiten des Industriegütermarketingsferase WbwB. All of these enzymes require acceptor substrates based on GalNAc-diphosphate-lipid. These methods are applicable for the assembly of bacterial polysaccharides of gram-negative bacteria that require sugar-diphosphate intermediates and are a basis for vaccine synthesis.作者: Crohns-disease 時間: 2025-3-26 23:50
https://doi.org/10.1007/978-3-531-92639-1aracterization of pathogen glycoconjugate biosynthetic pathways, and for the in vitro synthesis of human-like glycans produced by bacterial pathogens. The methods presented here should enable other assays to be developed for new pathway characterization.作者: 小卷發(fā) 時間: 2025-3-27 01:30 作者: Anthem 時間: 2025-3-27 08:12 作者: GIDDY 時間: 2025-3-27 12:17
Assay Methods for the Glycosyltransferases Involved in Synthesis of Bacterial Polysaccharides,aracterization of pathogen glycoconjugate biosynthetic pathways, and for the in vitro synthesis of human-like glycans produced by bacterial pathogens. The methods presented here should enable other assays to be developed for new pathway characterization.作者: Figate 時間: 2025-3-27 15:29 作者: 自戀 時間: 2025-3-27 18:52
Purification of Capsular Polysaccharide Complex from Gram-Negative Bacteria,e influence of the microbiota over immune system development and immune homeostasis, analysis of the antigens is more important than ever. Here we describe a method for the isolation of capsular polysaccharide from gram-negative bacteria, with the purification of polysaccharide from the commensal ba作者: Commodious 時間: 2025-3-27 23:45 作者: 領先 時間: 2025-3-28 03:58 作者: gorgeous 時間: 2025-3-28 10:11
Purification and Analysis of Mycobacterial Phosphatidylinositol Mannosides, Lipomannan, and Lipoaraies. PI can be further modified by glycan polymers, leading to the synthesis of PI mannosides (PIMs), lipomannan (LM), and lipoarabinomannan (LAM). Small lipids such as PI and PIMs are extracted with a mixture of chloroform, methanol, and water and analyzed by thin layer chromatography. For larger g作者: NAVEN 時間: 2025-3-28 14:04
Conjugation of Synthetic Oligosaccharides to Proteins by Squaric Acid Chemistry,quarate derivative of such oligosaccharide is formed first, which is followed by its reaction with a protein carrier. Monitoring of the conjugation reaction is achieved by SELDI-TOF-MS or MALDI-TOF-MS. This experimentally simple procedure yields desired glycoconjugates in high yields and with reprod作者: esoteric 時間: 2025-3-28 15:57
Direct Conjugation of Bacterial Polysaccharides to Proteins by Squaric Acid Chemistry,s a two-step process consisting of labeling the polysaccharide with a squarate group and a reaction of the squarate formed with protein. The intermediate squarate derivative and the product glycoconjugate can be easily purified using centrifugal filtration devices. This method is experimentally simp作者: 易受刺激 時間: 2025-3-28 21:36
Chemical Modification of the N-Acetyl Moieties of Hyaluronic Acid from , for Studies in Cytokine Prian systems. Here, we describe the treatment of bacterial hyaluronic acid by hydrazine or NaOH to yield smaller partially deacetylated polymers. These N-deacetylated polymers can be reacylated with acyl anhydrides to yield substituted hyaluronic acid derivatives of equivalent size and equimolar N-ac作者: 失敗主義者 時間: 2025-3-29 02:19 作者: 安撫 時間: 2025-3-29 03:45
Designing Glycosyltransferase Expression Constructs for Improved Purification, Protein Yield, and Cvariety of other domains, including those that help recruit them to the membrane. Structural and functional characterization of these proteins is often simplified by making a subconstruct that is better behaved in solution, and perhaps monofunctional. In this chapter we review bioinformatics tools a作者: Acquired 時間: 2025-3-29 08:03 作者: 宇宙你 時間: 2025-3-29 13:34 作者: GRAIN 時間: 2025-3-29 19:14 作者: 彩色的蠟筆 時間: 2025-3-29 20:25
Enzymatic Synthesis of Repeating Unit Oligosaccharides of , O104,arides of ECO104 consist of repeating units with the structure [4Galα1-4Neu5,7,9Ac3α2-3Galβ1-3GalNAcβ1-].. These repeating units are assembled sequentially by specific glycosyltransferases on a diphosphate-undecaprenol intermediate. Internal structures include mimics of the human T and sialyl-T anti作者: exercise 時間: 2025-3-30 01:01
Sulfhydryl Labeling as a Tool to Investigate the Topology of Membrane Proteins Involved in Lipopolygions, delimit the protein orientation in the membrane, the number of transmembrane segments, and the position of critical amino acids (whether exposed to the solvent or embedded in the lipid bilayer). Elucidating the topology of bacterial integral membrane proteins typically involves the constructi作者: Anthem 時間: 2025-3-30 06:16 作者: 是貪求 時間: 2025-3-30 11:24 作者: 笨拙處理 時間: 2025-3-30 15:27 作者: faucet 時間: 2025-3-30 19:31 作者: 完成才會征服 時間: 2025-3-30 23:35
Springer Science+Business Media, LLC, part of Springer Nature 2019作者: 全能 時間: 2025-3-31 02:30 作者: 果核 時間: 2025-3-31 08:22 作者: Instinctive 時間: 2025-3-31 11:53 作者: 我還要背著他 時間: 2025-3-31 17:03 作者: –DOX 時間: 2025-3-31 21:34
Conjugation of Synthetic Oligosaccharides to Proteins by Squaric Acid Chemistry,quarate derivative of such oligosaccharide is formed first, which is followed by its reaction with a protein carrier. Monitoring of the conjugation reaction is achieved by SELDI-TOF-MS or MALDI-TOF-MS. This experimentally simple procedure yields desired glycoconjugates in high yields and with reproducible hapten–protein ratios.作者: 整潔 時間: 2025-4-1 01:01 作者: 高度 時間: 2025-4-1 05:28 作者: 芭蕾舞女演員 時間: 2025-4-1 07:02 作者: ETCH 時間: 2025-4-1 11:36
RecET Direct Cloning of Polysaccharide Gene Cluster from Gram-Negative Bacteria,RecET direct cloning enables obtaining a large DNA region from genome. Here we describe its applications in cloning of polysaccharide gene cluster from gram-negative bacteria. Rapid and exact cloning of polysaccharide gene cluster can be achieved by this method.
