標(biāo)題: Titlebook: Bacterial Persistence; Methods and Protocol Jan Michiels,Maarten Fauvart Book 2016 Springer Science+Business Media New York 2016 Antibiotic [打印本頁(yè)] 作者: lumbar-puncture 時(shí)間: 2025-3-21 18:14
書(shū)目名稱Bacterial Persistence影響因子(影響力)
作者: 和諧 時(shí)間: 2025-3-21 22:55
https://doi.org/10.1007/978-3-531-19981-8 intracellular bacterial reservoirs, chronic bacterial cystitis, and recurrent infections. Urine, bladder, and kidney tissues can be analyzed by bacterial culture, histology, immunohistochemistry, immunofluorescent and confocal microscopy, electron microscopy, and flow cytometry, while a broad array作者: 詞匯表 時(shí)間: 2025-3-22 02:22 作者: minion 時(shí)間: 2025-3-22 05:54 作者: osteopath 時(shí)間: 2025-3-22 12:17
A Murine Model for , Urinary Tract Infection intracellular bacterial reservoirs, chronic bacterial cystitis, and recurrent infections. Urine, bladder, and kidney tissues can be analyzed by bacterial culture, histology, immunohistochemistry, immunofluorescent and confocal microscopy, electron microscopy, and flow cytometry, while a broad array作者: aerial 時(shí)間: 2025-3-22 15:00 作者: Hot-Flash 時(shí)間: 2025-3-22 19:13
Gudrun Quenzel,Klaus Hurrelmannata around the mean, making interpretation difficult. Furthermore, additional reproducibility problems arise from the lack of a standard method, different research groups using different protocols. Here, we describe a standard and optimized method for monitoring . persister cells at the population level allowing for maximal reproducibility.作者: laceration 時(shí)間: 2025-3-22 22:02 作者: Terrace 時(shí)間: 2025-3-23 01:51
Anke Grotlüschen,Klaus Buddebergular and genetic underpinnings of persister formation. We here provide an overview of the key studies that have paved the way for the current boom in persistence research, with a special focus on the technological and methodological advances that have enabled this progress.作者: 值得尊敬 時(shí)間: 2025-3-23 07:07
Psychologische Bildungsforschungantibiotics, significantly reduce the persister fraction in . The method is readily adaptable for other species. Further characterization and analysis of the mode of action of the identified compounds can provide additional insight into the mechanisms behind persister formation and can guide the development of future anti-persister therapies.作者: FLING 時(shí)間: 2025-3-23 11:52
Springer Reference Sozialwissenschaftenal ancestral level up to 100?%. This method will help to unravel molecular pathways to persistence, and opens up a myriad of new possibilities in persister research, such as the convenient study of nearly pure persister cultures and the possibility to investigate the role of time and environmental aspects in the evolution of persistence.作者: OVERT 時(shí)間: 2025-3-23 14:07 作者: Exposition 時(shí)間: 2025-3-23 20:33 作者: NEXUS 時(shí)間: 2025-3-23 23:04 作者: 笨重 時(shí)間: 2025-3-24 04:55 作者: debunk 時(shí)間: 2025-3-24 07:20
Optimized Method for Measuring Persistence in Escherichia coli with Improved Reproducibilityata around the mean, making interpretation difficult. Furthermore, additional reproducibility problems arise from the lack of a standard method, different research groups using different protocols. Here, we describe a standard and optimized method for monitoring . persister cells at the population level allowing for maximal reproducibility.作者: ostracize 時(shí)間: 2025-3-24 10:46
Protocol for Determination of the Persister Subpopulation in , Biofilms. Upon incubation, the fraction of surviving biofilm cells is determined by plating and plotted versus the used concentrations of the antifungal compound. If a persister subpopulation in the biofilm is present, the dose-dependent killing of the biofilm cells results in a biphasic killing pattern.作者: LEVY 時(shí)間: 2025-3-24 15:27
1064-3745 ation advice from the experts.This volume presents a comprehensive collection of methods that have been instrumental to the current understanding of bacterial persisters. Chapters in the book cover topics ranging from general methods for measuring persister levels in .Escherichia coli .cultures, pro作者: 完成 時(shí)間: 2025-3-24 19:20
Iris Pfeiffer,Simone Kaiser,Manon Rani Sharmompounds on biofilms. Stainings to evaluate the amount of biomass (crystal violet staining) and the number of metabolically active cells (resazurin assay) are discussed and specific attention is paid to the use of this model to quantify persisters in sessile populations.作者: BAIT 時(shí)間: 2025-3-25 01:16 作者: 嬉耍 時(shí)間: 2025-3-25 06:57
A Microplate-Based System as In Vitro Model of Biofilm Growth and Quantificationompounds on biofilms. Stainings to evaluate the amount of biomass (crystal violet staining) and the number of metabolically active cells (resazurin assay) are discussed and specific attention is paid to the use of this model to quantify persisters in sessile populations.作者: 大暴雨 時(shí)間: 2025-3-25 10:04
In Vitro Models for the Study of the Intracellular Activity of Antibioticsdel is described, consisting of (1) phagocytosis of pre-opsonized bacteria by eukaryotic cells; (2) elimination of non-internalized bacteria with gentamicin; (3) incubation of infected cells with antibiotics; and (4) determination of surviving bacteria by viable cell counting and normalization of the counts based on sample protein content.作者: BLAZE 時(shí)間: 2025-3-25 14:29
A Historical Perspective on Bacterial Persistencee so-called persister state. Persister cells are increasingly being viewed as a major cause of the recurrence of chronic infectious disease and could be an important factor in the emergence of antibiotic resistance. The phenomenon of persistence was first described in the 1940s, but remained poorly 作者: 謙虛的人 時(shí)間: 2025-3-25 16:37
A General Method for Measuring Persister Levels in , Culturesinvolved in the establishment of chronic infections. Persister levels depend on growth conditions. Here, we discuss the parameters that have to be considered when measuring persister levels and provide a sample protocol to do it.作者: Gyrate 時(shí)間: 2025-3-25 21:23
Optimized Method for Measuring Persistence in Escherichia coli with Improved Reproducibilityle But Non-Culturable Cells (VBNCs). To date, the predominant method consists of determining the survival rate of a bacterial population after antibiotic treatment as a function of time or antibiotic concentration. Unfortunately, this method is limited, as it shows high levels of dispersion of the d作者: 兩棲動(dòng)物 時(shí)間: 2025-3-26 00:12 作者: TOXIN 時(shí)間: 2025-3-26 04:27
Protocol for Determination of the Persister Subpopulation in , Biofilmstions of currently used antifungals. In this chapter, the method to determine the persister fraction in a . biofilm treated with an antifungal compound is described. To this end, a mature biofilm is developed and subsequently treated with a concentration series of the antifungal compound of interest作者: outer-ear 時(shí)間: 2025-3-26 09:46 作者: OCTO 時(shí)間: 2025-3-26 15:27 作者: Excise 時(shí)間: 2025-3-26 17:45
Single-Cell Detection and Collection of Persister Bacteria in a Directly Accessible Femtoliter Droplfabrication, femtoliter droplet array formation and concomitant enclosure of single cells, long-term culture and observation of single cells in droplets, and collection of identified persisters from single droplets are described in detail.作者: 粗糙濫制 時(shí)間: 2025-3-27 00:04 作者: 釋放 時(shí)間: 2025-3-27 02:12
Functional Analysis of the Role of Toxin–Antitoxin (TA) Loci in Bacterial Persistencebinant TA genes into inducible plasmids and expressing these in . Following expression, toxicity, resuscitation of growth, and changes in persister cell formation are assayed. This can confirm whether predicted TA loci are active in . and whether expression can affect persister cell formation.作者: 恃強(qiáng)凌弱的人 時(shí)間: 2025-3-27 08:34
Experimental Evolution of Escherichia coli Persister Levels Using Cyclic Antibiotic Treatmentsattempts have been made to elucidate persistence mechanisms. However, because of the challenges involved in studying persisters and the clear redundancy in mechanisms underlying their generation, our knowledge of molecular pathways to persistence remains incomplete. Here, we describe how to use expe作者: VERT 時(shí)間: 2025-3-27 11:16
In Vitro Models for the Study of the Intracellular Activity of Antibioticspotent or effective against intracellular bacteria as well as to explore the reasons for poor bacterial responsiveness. An in vitro pharmacodynamic model is described, consisting of (1) phagocytosis of pre-opsonized bacteria by eukaryotic cells; (2) elimination of non-internalized bacteria with gent作者: Iniquitous 時(shí)間: 2025-3-27 14:06 作者: 范例 時(shí)間: 2025-3-27 21:27
https://doi.org/10.1007/978-1-4939-2854-5Antibiotics; Bacteria; Mycobacterium Tuberculosis; Persister Cells; Pseudomonas Aeruginosa; Staphylococcu作者: mydriatic 時(shí)間: 2025-3-27 22:49
978-1-4939-4904-5Springer Science+Business Media New York 2016作者: 生銹 時(shí)間: 2025-3-28 03:23 作者: Irascible 時(shí)間: 2025-3-28 07:24
Persisters: Methods for Isolation and Identifying Contributing Factors—A ReviewPersister cells are phenotypic variants surviving a lethal dose of antibiotic, sufficient to kill the bulk of an exponential phase population. In this chapter we summarize current techniques to isolate persisters and discuss limitations associated with identifying mechanisms of persister formation.作者: 隼鷹 時(shí)間: 2025-3-28 14:28 作者: ENNUI 時(shí)間: 2025-3-28 16:23
Beatrice Rammstedt,Anouk Zabal,Britta Gaulyinvolved in the establishment of chronic infections. Persister levels depend on growth conditions. Here, we discuss the parameters that have to be considered when measuring persister levels and provide a sample protocol to do it.作者: FOIL 時(shí)間: 2025-3-28 22:12
Gender Budgeting in der Bildungsfinanzierungnating between slow growers that are generated by exposure to a stress signal (Type I persisters) and slow growers that are continuously generated during exponential growth (Type II persisters). Second, the method enables determining the amount of slow growers in a culture.作者: Missile 時(shí)間: 2025-3-28 23:17 作者: 問(wèn)到了燒瓶 時(shí)間: 2025-3-29 06:19 作者: 皮薩 時(shí)間: 2025-3-29 07:52
Jan Michiels,Maarten FauvartIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: 擦試不掉 時(shí)間: 2025-3-29 12:23 作者: micronized 時(shí)間: 2025-3-29 16:34 作者: 突變 時(shí)間: 2025-3-29 20:40 作者: slow-wave-sleep 時(shí)間: 2025-3-30 03:12 作者: 切碎 時(shí)間: 2025-3-30 04:21
Iris Pfeiffer,Simone Kaiser,Manon Rani Sharmcial systems and thus significantly differ from in vivo conditions, they represent an important tool to evaluate biofilm formation and the effect of compounds on biofilms. Stainings to evaluate the amount of biomass (crystal violet staining) and the number of metabolically active cells (resazurin as作者: 無(wú)關(guān)緊要 時(shí)間: 2025-3-30 11:05
https://doi.org/10.1007/978-3-531-92520-2tions of currently used antifungals. In this chapter, the method to determine the persister fraction in a . biofilm treated with an antifungal compound is described. To this end, a mature biofilm is developed and subsequently treated with a concentration series of the antifungal compound of interest作者: 后天習(xí)得 時(shí)間: 2025-3-30 15:55 作者: 意見(jiàn)一致 時(shí)間: 2025-3-30 17:09
Stefan Hummelsheim,Dieter Timmermanninfections, and therefore, understanding their physiology may lead to novel therapeutics to treat recalcitrant infections. However, persisters have yet to be isolated due to their low abundance, transient nature, and similarity to the more highly abundant viable but non-culturable cells (VBNCs), res作者: transdermal 時(shí)間: 2025-3-30 22:21
Wilfried Bos,T. Neville Postlethwaitefabrication, femtoliter droplet array formation and concomitant enclosure of single cells, long-term culture and observation of single cells in droplets, and collection of identified persisters from single droplets are described in detail.作者: Reverie 時(shí)間: 2025-3-31 03:34
Psychologische Bildungsforschungd persister cell formation. Research in this field has mainly focused on the model organism . and few genetic determinants of persistence have been described in other bacterial species, impairing the development of target-based strategies to combat these antibiotic-tolerant cells. In this chapter we作者: 純樸 時(shí)間: 2025-3-31 06:22
Wilfried Bos,T. Neville Postlethwaitebinant TA genes into inducible plasmids and expressing these in . Following expression, toxicity, resuscitation of growth, and changes in persister cell formation are assayed. This can confirm whether predicted TA loci are active in . and whether expression can affect persister cell formation.
