作者: 主動(dòng) 時(shí)間: 2025-3-21 21:33 作者: 砍伐 時(shí)間: 2025-3-22 03:05
https://doi.org/10.1007/978-3-642-58196-0om its chaperone. Furthermore, we detail the production of CagL and the use of differential scanning fluorimetry to identify how CagL is thermally stabilized by reduced pH, which led to a new crystal form of CagL and novel insight to pathogenic mechanisms. The methods described here for the producti作者: 小平面 時(shí)間: 2025-3-22 05:19 作者: Canary 時(shí)間: 2025-3-22 09:34
Differential Radial Capillary Action of Ligand Assay (DRaCALA) for High-Throughput Detection of Prot by DRaCALA in a 96-well format to allow rapid identification of protein–ligand interactions, including previously unknown proteins. Here, we present the protocols for generating the expression library, conducting the DRaCALA screen, data analysis, and hit validation.作者: Ingest 時(shí)間: 2025-3-22 14:12 作者: SEVER 時(shí)間: 2025-3-22 19:11 作者: cortisol 時(shí)間: 2025-3-22 22:36
1064-3745 n vivo modeling of bacterial infections; and Part 6 explores how bacterial pathogens are the true experts of the immune system. Written?in the highly successful?.Methods in Molecular Biology.?series format, cha978-1-4939-8268-4978-1-4939-6673-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Blasphemy 時(shí)間: 2025-3-23 04:50
Gestaltung von Arbeit und Technikion that was previously hard to extract with traditional methods. Here, we present basic concepts of automated image processing, segmentation and analysis that can be relatively easy implemented for use with bacterial research.作者: medieval 時(shí)間: 2025-3-23 09:06
Computer Vision-Based Image Analysis of Bacteriaion that was previously hard to extract with traditional methods. Here, we present basic concepts of automated image processing, segmentation and analysis that can be relatively easy implemented for use with bacterial research.作者: 委屈 時(shí)間: 2025-3-23 11:05 作者: 驚奇 時(shí)間: 2025-3-23 14:53
Handbuch Betriebliche Kreislaufwirtschaftecularly guided imaging. Our goal in providing a detailed protocol of our efforts is to enable others to build upon this methodology to answer pertinent translational and basic research questions in the pursuit of diagnosis and treatment of infective endocarditis.作者: 失敗主義者 時(shí)間: 2025-3-23 20:25 作者: GROSS 時(shí)間: 2025-3-23 22:17 作者: 帶來(lái)墨水 時(shí)間: 2025-3-24 04:49
Protein-Based Strategies to Identify and Isolate Bacterial Virulence Factorsdentification of both unknown host and bacterial factors. The methods described have among others successfully been used to identify and characterize several IgG-binding proteins, including protein G, protein H, and protein L.作者: insolence 時(shí)間: 2025-3-24 09:57
Identifying Bacterial Immune Evasion Proteins Using Phage Displayy. Genomic bacterial DNA is randomly fragmented and ligated into a phage display vector that is used to create a phage display library expressing bacterial secreted and membrane bound proteins. This library is used to select displayed bacterial secretome proteins that interact with host immune components.作者: 把手 時(shí)間: 2025-3-24 14:45
Competition for Iron Between Host and Pathogen: A Structural Case Study on ,rowth. The competition for iron is the central battlefield between pathogen and host. This mini-review summarizes the updated scenarios of the battle for iron between . and human host from a structural biology perspective.作者: 整體 時(shí)間: 2025-3-24 17:21 作者: FLAG 時(shí)間: 2025-3-24 21:19 作者: reptile 時(shí)間: 2025-3-25 01:20
https://doi.org/10.1007/978-3-658-06447-1dentification of both unknown host and bacterial factors. The methods described have among others successfully been used to identify and characterize several IgG-binding proteins, including protein G, protein H, and protein L.作者: Prosaic 時(shí)間: 2025-3-25 03:47 作者: 偽造 時(shí)間: 2025-3-25 08:08 作者: 效果 時(shí)間: 2025-3-25 13:30
Bernd-Joachim Ertelt,William E. Schulze majority of species where recombination is frequent. Here, we review and describe the computational techniques currently available to infer ancestral relationships, including phylogenetic methods that either ignore or account for the effect of recombination, as well as model-based and model-free phylogeny-independent approaches.作者: 蝕刻術(shù) 時(shí)間: 2025-3-25 18:51
Nationales Kreislaufwirtschaftsrecht,meabilization. This protocol allows a high level of morphological preservation, suitable for high-resolution microscopy. With slight modification, this protocol could also be used for other Gram-positive pathogens.作者: 其他 時(shí)間: 2025-3-25 20:37
Book 2017s., .Staphylococcus aureus., .Streptococcus pneumonia., .Listeria monocytogenes.,?and?.M.ycobacterium marinum.. .Bacterial Pathogens: Methods and Protocols.?is divided into 6 parts: Part 1 describes different approaches to identifying and characterizing bacterial effector molecules; Part 2 deals wit作者: Tempor 時(shí)間: 2025-3-26 01:23
1064-3745 tion advice from the experts.Includes supplementary material.This volume discusses various methods and protocols used for the experimentation of a wide range of bacterial species, such as?.Streptococcus pyogenes., .Staphylococcus aureus., .Streptococcus pneumonia., .Listeria monocytogenes.,?and?.M.y作者: inconceivable 時(shí)間: 2025-3-26 07:48 作者: 先兆 時(shí)間: 2025-3-26 10:33
Bildungsberatung in der Weiterbildungult in motion blur during image acquisition. Here, we describe two techniques of sample preparation that reduce unwanted cell movement and are suitable for application to a number of bacterial species and imaging methods.作者: 撫育 時(shí)間: 2025-3-26 14:27
Assessing Vacuolar Escape of ,quantitatively measuring . infection and escape from its internalization vacuole, as well as a correlative light/electron microscopy method to investigate the morphological features of the vacuolar compartments containing ..作者: STRIA 時(shí)間: 2025-3-26 19:22
Immobilization Techniques of Bacteria for Live Super-resolution Imaging Using Structured Illuminatioult in motion blur during image acquisition. Here, we describe two techniques of sample preparation that reduce unwanted cell movement and are suitable for application to a number of bacterial species and imaging methods.作者: Enervate 時(shí)間: 2025-3-26 23:53 作者: 護(hù)身符 時(shí)間: 2025-3-27 02:39 作者: 鍍金 時(shí)間: 2025-3-27 09:11 作者: 會(huì)議 時(shí)間: 2025-3-27 09:35 作者: Intractable 時(shí)間: 2025-3-27 15:03
Competition for Iron Between Host and Pathogen: A Structural Case Study on ,uch an acidic and inhospitable niche, . cells have evolved complex mechanisms to acquire nutrients from human hosts, including iron, an essential nutrient for both the pathogens and host cells. However, human cells also utilize diverse strategies in withholding of irons to prevent the bacterial outg作者: diathermy 時(shí)間: 2025-3-27 18:28 作者: 檢查 時(shí)間: 2025-3-27 22:57
Reconstructing the Ancestral Relationships Between Bacterial Pathogen Genomesvely low cost. Analyzing this growing wealth of genomic data in terms of ancestral relationships can reveal many interesting aspects of the evolution, ecology, and epidemiology of bacterial pathogens. However, reconstructing the ancestry of a sample of bacteria remains challenging, especially for th作者: Heresy 時(shí)間: 2025-3-28 02:26
Making Fluorescent Streptococci and Enterococci for Live Imagingvastly different areas of life sciences have been developed. To optimize GFP for diverse life science applications, a large variety of GFP derivatives with different environmental characteristics have been generated by mutagenesis. The enhanced green fluorescent protein (EGFP) is a well-known GFP de作者: largesse 時(shí)間: 2025-3-28 09:34 作者: 千篇一律 時(shí)間: 2025-3-28 13:30 作者: Encumber 時(shí)間: 2025-3-28 17:43 作者: 虛弱的神經(jīng) 時(shí)間: 2025-3-28 19:39 作者: 放氣 時(shí)間: 2025-3-28 23:19
Detection of Intracellular Proteins by High-Resolution Immunofluorescence Microscopy in ,osed antigens can easily be determined using fluorescent antibodies, localization of intracellular antigens requires permeabilization of the bacterial cell wall and membrane. Here, we describe an immunofluorescence protocol tailored specifically for ., applying the phage lysin PlyC for cell wall per作者: Hangar 時(shí)間: 2025-3-29 05:17 作者: 誤傳 時(shí)間: 2025-3-29 11:13
Bacterial Pathogenesis978-1-4939-6673-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Antecedent 時(shí)間: 2025-3-29 15:25 作者: Estrogen 時(shí)間: 2025-3-29 19:26
Analysis of Bacterial Surface Interactions with Mass Spectrometry-Based Proteomicsproteins attached to the bacterial surface followed by quantitative mass spectrometry based proteomics analysis. This technique provides an overview of the host–pathogen interaction network, which can be used to guide directed perturbations of the system, and to select target of specific interest for further studies.作者: 舊石器 時(shí)間: 2025-3-29 22:40 作者: 劇本 時(shí)間: 2025-3-30 00:11 作者: breadth 時(shí)間: 2025-3-30 07:09
Pontus Nordenfelt,Mattias CollinIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains keynotes and implementation advice from the experts.Includes supplementary material作者: 占線 時(shí)間: 2025-3-30 10:12
Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/180308.jpg作者: Hyperlipidemia 時(shí)間: 2025-3-30 13:36 作者: osteoclasts 時(shí)間: 2025-3-30 19:10
978-1-4939-8268-4Springer Science+Business Media New York 2017作者: CLAMP 時(shí)間: 2025-3-30 22:30 作者: Tonometry 時(shí)間: 2025-3-31 02:06
Bausparen im deutschen Retail-Marktproteins attached to the bacterial surface followed by quantitative mass spectrometry based proteomics analysis. This technique provides an overview of the host–pathogen interaction network, which can be used to guide directed perturbations of the system, and to select target of specific interest fo作者: intricacy 時(shí)間: 2025-3-31 08:54 作者: wangle 時(shí)間: 2025-3-31 10:35
Bausparen im deutschen Retail-Marktroteins or use a proteomics driven approach. Although proven successful these methods are limited by a low efficiency and or lack of functional identification. Here we describe a high-throughput genomic strategy to functionally identify bacterial immune evasion proteins using phage display technolog作者: 歌曲 時(shí)間: 2025-3-31 14:27
https://doi.org/10.1007/978-3-642-58196-0uch an acidic and inhospitable niche, . cells have evolved complex mechanisms to acquire nutrients from human hosts, including iron, an essential nutrient for both the pathogens and host cells. However, human cells also utilize diverse strategies in withholding of irons to prevent the bacterial outg作者: 易于交談 時(shí)間: 2025-3-31 20:26
