作者: PLE 時(shí)間: 2025-3-22 00:13
Comparative Analyses of Extrachromosomal Bacterial Replicons, Identification of Chromids, and Expers with properties of both chromosomes and plasmids). Comparative analyses of bacterial plasmids, including homology searches, phylogenetic and phylogenomic analyses, as well as network construction for the characterization of their relationships, are good starting points for the identification of ch作者: 內(nèi)閣 時(shí)間: 2025-3-22 00:53 作者: GOAD 時(shí)間: 2025-3-22 06:58
The Pyrosequencing Protocol for Bacterial Genomes,way of DNA sequencing. In the last years the same strategy grew up and was technologically updated, reaching a high throughput in terms of amount of generated sequences (reads) per run and in terms of length of sequence up to values of 800–1,000 bases. These features of pyrosequencing perfectly fit 作者: 改變 時(shí)間: 2025-3-22 12:05 作者: 土坯 時(shí)間: 2025-3-22 13:22
The Illumina-Solexa Sequencing Protocol for Bacterial Genomes,ches spanning entire genomes, with the latest instruments capable of producing hundreds of gigabases of data in a single sequencing run. Illumina’s NGS instruments powerfully combine the flexibility of single reads with short- and long-insert paired-end reads, and enable a wide range of DNA sequenci作者: faucet 時(shí)間: 2025-3-22 18:49
High-Throughput Phenomics,ss, there is the need to pay attention to several crucial steps in order to obtain high-quality and reproducible data from PM, such as the choice of the Dye mix, the type and concentration of the carbon source in metabolic experiments, the use of a buffered medium. A systematic research of auxotroph作者: 下邊深陷 時(shí)間: 2025-3-22 23:09 作者: 他一致 時(shí)間: 2025-3-23 02:19
Raw Sequence Data and Quality Control,f data, is the analysis of raw sequence quality and removal (trimming) of low-quality segments while retaining sufficient information for subsequent analyses. Here, we present a series of methods useful for converting and for refinishing one or more sequence files. One of the methods proposed, based作者: 肌肉 時(shí)間: 2025-3-23 06:10 作者: 變化 時(shí)間: 2025-3-23 09:58
Mapping Contigs Using CONTIGuator,ncreasing number of bacterial species having more than one strain sequenced, thus facilitating the annotation process. On the other hand, many genomic sequences are now left in the “draft” status, as a series of contigs, mainly for the labor-intensive finishing task. As a result, many genomic analys作者: Visual-Acuity 時(shí)間: 2025-3-23 16:23 作者: 事情 時(shí)間: 2025-3-23 21:53
Defining Orthologs and Pangenome Size Metrics,asingly more genomes from microbes belonging to the same taxonomic unit. Eventually, this led to the concept of ., that is, the entire set of genes present in a group of representatives of the same genus/species, which, in turn, can be divided into ., defined as the set of those genes present in all作者: Expertise 時(shí)間: 2025-3-23 23:55 作者: 種子 時(shí)間: 2025-3-24 06:17
Genome-Scale Metabolic Network Reconstruction,oit new usable substances of microbial origin. Computational modeling and in silico simulations are powerful tools in this context since they allow the exploration and a deeper understanding of bacterial metabolic circuits. Many approaches exist to quantitatively simulate chemical reaction fluxes wi作者: 老人病學(xué) 時(shí)間: 2025-3-24 09:38 作者: Inordinate 時(shí)間: 2025-3-24 12:37
Genome-Wide Detection of Selection and Other Evolutionary Forces,ions occur frequently during the long-term pathogen–host evolutionary arms race, and individual mutation beneficial for the fitness can be fixed preferentially. Many recent comparative genomics studies have pointed out the importance of selective forces in the molecular evolution of bacterial pathog作者: Little 時(shí)間: 2025-3-24 16:37
The Integrated Microbial Genome Resource of Analysis,arative analysis of microbial genomes and metagenomes. IMG has been developed by the US Department of Energy (DOE)-Joint Genome Institute (JGI). IMG platform contains both draft and complete genomes, sequenced by Joint Genome Institute and other public and available genomes. Genomes of strains belon作者: Spinal-Fusion 時(shí)間: 2025-3-24 21:56
1064-3745 ation advice from the experts.Bacterial genomics is a mature research interdisciplinary field, which is approached by ecologists, geneticists, bacteriologists, molecular biologists and evolutionary biologists working in medical, industrial and basic science.?Thanks to the large diffusion of bacteria作者: SKIFF 時(shí)間: 2025-3-24 23:36 作者: Watemelon 時(shí)間: 2025-3-25 06:06 作者: Commodious 時(shí)間: 2025-3-25 10:14
Raw Sequence Data and Quality Control,nalyses. Here, we present a series of methods useful for converting and for refinishing one or more sequence files. One of the methods proposed, based on dynamic trimming, as implemented in the software StreamingTrim allows a fast and accurate trimming of sequence files, with low memory requirement.作者: 流眼淚 時(shí)間: 2025-3-25 14:01 作者: Spinal-Tap 時(shí)間: 2025-3-25 16:04 作者: Hirsutism 時(shí)間: 2025-3-25 23:05 作者: 生氣地 時(shí)間: 2025-3-26 03:58
https://doi.org/10.1007/978-3-658-21704-4on, analyze and graphically represent the pan-genome structure, and identify lineage-specific gene families for the 12 complete pIncA/C plasmids currently available in NCBI’s RefSeq. The software package, license, and detailed user manual can be downloaded for free for academic use from two mirrors: . and ..作者: seruting 時(shí)間: 2025-3-26 06:55 作者: 肌肉 時(shí)間: 2025-3-26 10:36 作者: 遣返回國 時(shí)間: 2025-3-26 14:19 作者: placebo 時(shí)間: 2025-3-26 16:50 作者: etiquette 時(shí)間: 2025-3-27 00:19 作者: Restenosis 時(shí)間: 2025-3-27 02:01
Wolfgang Schroeder,Bernhard We?elsto bacterial genome sequencing for the de novo assemblies and resequencing as well. The approaches of shotgun and paired ends sequencing allow the bacterial genome finishing providing a high-quality data in few days with unprecedented results.作者: 羞辱 時(shí)間: 2025-3-27 07:12
https://doi.org/10.1007/978-3-531-92452-6ng applications. Here, we describe the paired-end library preparation with an average insert size of 470 bp, 2 kbp, and 6 kbp, together with the DNA cluster generation and sequencing procedure of . O104:H4 genome on Illumina Hiseq 2000 platform.作者: 遺傳學(xué) 時(shí)間: 2025-3-27 11:44
Wolfgang Schroeder,Bernhard We?els We will use data from two . serovar Typhimurium strains as an example here, strain LT2 and strain 14028S, to assess if there are orthologous gene pairs with different expression domains related in both strains.作者: 機(jī)械 時(shí)間: 2025-3-27 17:41 作者: 加入 時(shí)間: 2025-3-27 18:13
Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/180304.jpg作者: Indict 時(shí)間: 2025-3-28 00:25 作者: 離開真充足 時(shí)間: 2025-3-28 06:07
978-1-4939-5547-3Springer Science+Business Media New York 2015作者: Myocyte 時(shí)間: 2025-3-28 09:05 作者: 有偏見 時(shí)間: 2025-3-28 13:42 作者: 洞察力 時(shí)間: 2025-3-28 18:09 作者: refraction 時(shí)間: 2025-3-28 19:14 作者: notion 時(shí)間: 2025-3-29 02:51 作者: 討好女人 時(shí)間: 2025-3-29 06:15 作者: irreducible 時(shí)間: 2025-3-29 09:07
Eike Bohlken PD Dr.,Christian Thies PD Dr. beginning of the twenty-first century. Thanks to the possibility to produce large amount of sequence data, these tools are going to completely substitute other high-throughput technologies. Moreover, the large applications of NGS protocols are increasing the genetic decoding of biological systems t作者: 認(rèn)為 時(shí)間: 2025-3-29 12:28 作者: 先鋒派 時(shí)間: 2025-3-29 16:03 作者: 平靜生活 時(shí)間: 2025-3-29 22:43
https://doi.org/10.1007/978-3-531-92452-6ches spanning entire genomes, with the latest instruments capable of producing hundreds of gigabases of data in a single sequencing run. Illumina’s NGS instruments powerfully combine the flexibility of single reads with short- and long-insert paired-end reads, and enable a wide range of DNA sequenci作者: Adjourn 時(shí)間: 2025-3-30 02:33
https://doi.org/10.1007/978-3-531-92452-6ss, there is the need to pay attention to several crucial steps in order to obtain high-quality and reproducible data from PM, such as the choice of the Dye mix, the type and concentration of the carbon source in metabolic experiments, the use of a buffered medium. A systematic research of auxotroph作者: strain 時(shí)間: 2025-3-30 04:13
Wolfgang Schroeder,Bernhard We?elsan display different lifestyles and it is still not well known to what extent the core pangenome plays a role in the divergence of lifestyles between the two organisms. Here, we present a procedure for uncovering the conservation and divergence of gene expression by using large expression compendia.作者: Cougar 時(shí)間: 2025-3-30 11:01
https://doi.org/10.1007/978-3-658-08176-8f data, is the analysis of raw sequence quality and removal (trimming) of low-quality segments while retaining sufficient information for subsequent analyses. Here, we present a series of methods useful for converting and for refinishing one or more sequence files. One of the methods proposed, based作者: watertight, 時(shí)間: 2025-3-30 16:12
OT-Mitgliedschaften und OT-Verb?ndewadays it is an almost routine practice in many molecular biology labs. In this chapter we discuss in depth the various methods to assemble the short sequences (called reads) obtained from a massive sequencing system, using different software and strategies, and how to perform some fundamental quali作者: 織布機(jī) 時(shí)間: 2025-3-30 19:50
https://doi.org/10.1007/978-3-658-08176-8ncreasing number of bacterial species having more than one strain sequenced, thus facilitating the annotation process. On the other hand, many genomic sequences are now left in the “draft” status, as a series of contigs, mainly for the labor-intensive finishing task. As a result, many genomic analys作者: 感激小女 時(shí)間: 2025-3-30 21:37
Handbuch Arbeits- und Gesundheitsschutzal for obtaining scientific findings and new knowledge. Thus, bacterial genome annotation has emerged as a key point to investigate in bacteria. Any efficient tool designed specifically to annotate bacterial genomes sequenced with massively parallel technologies has to consider the specific features作者: 周年紀(jì)念日 時(shí)間: 2025-3-31 04:46
Handbuch Arbeits- und Gesundheitsschutzasingly more genomes from microbes belonging to the same taxonomic unit. Eventually, this led to the concept of ., that is, the entire set of genes present in a group of representatives of the same genus/species, which, in turn, can be divided into ., defined as the set of those genes present in all作者: 松緊帶 時(shí)間: 2025-3-31 05:34 作者: beta-carotene 時(shí)間: 2025-3-31 12:00
Finanzm?rkte und Finanzialisierungoit new usable substances of microbial origin. Computational modeling and in silico simulations are powerful tools in this context since they allow the exploration and a deeper understanding of bacterial metabolic circuits. Many approaches exist to quantitatively simulate chemical reaction fluxes wi作者: Console 時(shí)間: 2025-3-31 15:20
https://doi.org/10.1007/978-3-658-21704-4day’s biology. Such task is of paramount importance towards the understanding of biotechnologically relevant pathways and possibly for their manipulation. Fundamental prerequisites are the genome-wide reconstruction of metabolic pathways and a comprehensive measurement of cellular phenotypes. Cellul作者: 去掉 時(shí)間: 2025-3-31 18:18 作者: 魅力 時(shí)間: 2025-4-1 00:01 作者: DECRY 時(shí)間: 2025-4-1 05:17 作者: profligate 時(shí)間: 2025-4-1 08:51 作者: pancreas 時(shí)間: 2025-4-1 11:52
Methods for Assembling Reads and Producing Contigs,wadays it is an almost routine practice in many molecular biology labs. In this chapter we discuss in depth the various methods to assemble the short sequences (called reads) obtained from a massive sequencing system, using different software and strategies, and how to perform some fundamental quality controls on the data obtained.作者: JOG 時(shí)間: 2025-4-1 16:29
Book 2015ucible protocols and notes on troubleshooting and avoiding known pitfalls..Authoritative and easily accessible, .Bacterial Pangenomics: Methods and Protocols. will serve as a field guide for both qualified bacterial genomics investigators who want to update their technical knowledge, for less experi作者: 魅力 時(shí)間: 2025-4-1 19:12
https://doi.org/10.1007/978-3-658-25978-5Further tests examining growth in various media are used to distinguish secondary chromids from plasmids, and mutational analysis (e.g., using the yeast FLP/FRT recombination system) is employed to identify essential genes carried by particular chromids.
