標(biāo)題: Titlebook: Bacterial Chromatin; Methods and Protocol Remus T. Dame Book 2018 Springer Science+Business Media, LLC, part of Springer Nature 2018 nucleo [打印本頁] 作者: audiogram 時(shí)間: 2025-3-21 17:20
書目名稱Bacterial Chromatin影響因子(影響力)
書目名稱Bacterial Chromatin影響因子(影響力)學(xué)科排名
書目名稱Bacterial Chromatin網(wǎng)絡(luò)公開度
書目名稱Bacterial Chromatin網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Bacterial Chromatin被引頻次
書目名稱Bacterial Chromatin被引頻次學(xué)科排名
書目名稱Bacterial Chromatin年度引用
書目名稱Bacterial Chromatin年度引用學(xué)科排名
書目名稱Bacterial Chromatin讀者反饋
書目名稱Bacterial Chromatin讀者反饋學(xué)科排名
作者: cancer 時(shí)間: 2025-3-22 00:11 作者: 并入 時(shí)間: 2025-3-22 03:59 作者: definition 時(shí)間: 2025-3-22 06:12 作者: Grasping 時(shí)間: 2025-3-22 11:42 作者: ELUDE 時(shí)間: 2025-3-22 16:58 作者: Disk199 時(shí)間: 2025-3-22 18:06 作者: 托運(yùn) 時(shí)間: 2025-3-22 22:57 作者: THROB 時(shí)間: 2025-3-23 02:48 作者: 反饋 時(shí)間: 2025-3-23 06:37
Protein-Based Materials in Cosmeticspled with ligation-mediated polymerase chain reaction (LM-PCR) and Southern blot analysis. As an example we apply these in vivo protein-mapping methods to . to show direct binding of architectural proteins in the Lac repressor-mediated DNA repression loop.作者: 我正派 時(shí)間: 2025-3-23 12:20 作者: Toxoid-Vaccines 時(shí)間: 2025-3-23 14:34 作者: 滔滔不絕的人 時(shí)間: 2025-3-23 20:51
Book 2018ive and cutting-edge,?Bacterial Chromatin: Methods and Protocols?aims to be.?.useful as an up-to-date reference work for scholars in the bacterial chromatin field, those entering the field from adjacent research fields, and scientists in the eukaryotic chromatin field..作者: 懦夫 時(shí)間: 2025-3-24 02:04 作者: Minuet 時(shí)間: 2025-3-24 02:38
Atomic Force Microscopy Imaging and Analysis of Prokaryotic Genome Organizationn for Dps in ., and TrmBL2 in .. These systems are described here as examples of the successful application of AFM for this purpose. Moreover, we describe the procedures needed for quantitative analysis of the data.作者: 憤怒事實(shí) 時(shí)間: 2025-3-24 09:38 作者: 盤旋 時(shí)間: 2025-3-24 13:31
Determination of the 3D Genome Organization of Bacteria Using Hi-Cns. By combining 3C and high-throughput sequencing, the Hi-C method reveals genome-wide interactions within topological domains and global genome structure as a whole. This chapter provides detailed guidelines for the preparation of Hi-C sequencing libraries for bacteria.作者: BARGE 時(shí)間: 2025-3-24 17:25 作者: originality 時(shí)間: 2025-3-24 20:55 作者: 高度表 時(shí)間: 2025-3-25 02:55
Dynamic Light Scattering of DNA-Ligand Complexesion of its surface negative charges. In this chapter, we describe in detail the protocol for DLS of DNA-ligand complexes. As an example, we describe data for condensation of DNA by chitosan and the measurement of size, zeta potential, and electrophoretic mobility of the DNA-ligand complex by DLS.作者: 索賠 時(shí)間: 2025-3-25 06:39 作者: 嫻熟 時(shí)間: 2025-3-25 08:25
https://doi.org/10.1007/978-1-4615-5295-6mple and effective method named .nome .ootprinting with high-throughput .uencing (GeF-seq) to determine binding sites of DBPs at single base-pair resolution. GeF-seq detects binding sites of DBPs as sharp peaks and thus makes it possible to identify the recognition sequence in each “binding peak” more easily and accurately than using ChIP-seq.作者: brassy 時(shí)間: 2025-3-25 11:53
The Historian’s Craft in the “Periphery”s makes it possible to measure binding properties. We use the bacterial protein Integration Host Factor (IHF) as an example to show how specific binding to DNA can be measured. Moreover, we show a new intuitive quantitative approach to displaying data obtained via TPM.作者: helper-T-cells 時(shí)間: 2025-3-25 17:35 作者: Kidnap 時(shí)間: 2025-3-25 20:40 作者: PANEL 時(shí)間: 2025-3-26 02:15 作者: DIKE 時(shí)間: 2025-3-26 07:49
Observing Bacterial Chromatin Protein-DNA Interactions by Combining DNA Flow-Stretching with Single-w the binding of bacterial chromatin proteins can be correlated with DNA condensation. Lastly, we describe the DNA motion capture assay, which allows one to probe the mechanism of DNA condensation by tracking how different segments of a flow stretched DNA are compacted by bacterial chromatin proteins.作者: hermetic 時(shí)間: 2025-3-26 12:21 作者: sphincter 時(shí)間: 2025-3-26 13:42 作者: 使更活躍 時(shí)間: 2025-3-26 18:43
Imaging of Transcription and Replication in the Bacterial Chromosome with Multicolor Three-Dimensionteins, (2) imaging the transcription and replication machineries at single-cell levels, (3) performing imaging experiments to capture the spatial organization of the transcription machinery and the nucleoid, and (4) image acquisition and analysis.作者: Hormones 時(shí)間: 2025-3-26 21:26 作者: insightful 時(shí)間: 2025-3-27 01:38 作者: obsession 時(shí)間: 2025-3-27 06:18 作者: 證實(shí) 時(shí)間: 2025-3-27 09:31 作者: BLANC 時(shí)間: 2025-3-27 14:40 作者: Musculoskeletal 時(shí)間: 2025-3-27 19:29
Determination of the 3D Genome Organization of Bacteria Using Hi-Cal genomes are organized into insulated domains similar to the Topologically Associating Domains (TADs) detected in eukaryotic cells. Chromosome conformation capture (3C) technologies are used to analyze in vivo DNA proximity based on ligation of distal DNA segments crossed-linked by bridging protei作者: 俗艷 時(shí)間: 2025-3-27 22:13 作者: 越自我 時(shí)間: 2025-3-28 05:46 作者: 小母馬 時(shí)間: 2025-3-28 08:05
Genomic SELEX Screening of Regulatory Targets of , Transcription Factorsf protein-protein interaction: at the first step, seven species of the sigma subunit are involved, at the second step, a total of approximately 300 species of transcription factor (TFs). For the identification of the regulatory targets of these two groups of regulatory proteins, we developed two in 作者: myopia 時(shí)間: 2025-3-28 10:31
Modular Assembly of Synthetic Secondary Chromosomesrch and biotechnological applications. Questions of how chromosomes need to be constructed to maintain the genetic information can now be answered by a learning-by-building approach. Here, we describe an efficient pipeline for the design and assembly of synthetic, secondary chromosomes in . based on作者: judiciousness 時(shí)間: 2025-3-28 15:02 作者: Stable-Angina 時(shí)間: 2025-3-28 21:04
Imaging of Transcription and Replication in the Bacterial Chromosome with Multicolor Three-Dimensionrevealing “bacterial nucleolus-like structure or organization,” “nucleolus-like compartmentalization of the transcription factories,” and “spatial segregation of the transcription and replication machineries” have enhanced our understanding of the dynamic landscape of the bacterial chromatin. This c作者: enfeeble 時(shí)間: 2025-3-28 23:34
Atomic Force Microscopy Imaging and Analysis of Prokaryotic Genome Organization a simple cell manipulation procedure that enables step-wise dissection of the nucleoid. The procedure includes (1) on-substrate-lysis of cells, and (2) enzyme treatment, followed by atomic force microscopy. This type of dissection analysis permits analysis of nucleoid structure ranging from the fun作者: 詞匯 時(shí)間: 2025-3-29 05:27 作者: 刺激 時(shí)間: 2025-3-29 11:15 作者: FILLY 時(shí)間: 2025-3-29 12:19
Quantitative Determination of DNA Bridging Efficiency of Chromatin Proteinslices. Detecting DNA bridge formation generally involves the use of complex single-molecule techniques (atomic force microscopy, magnetic, or optical tweezers). Although DNA bridging can be qualitatively described, quantification of DNA bridging and bridging dynamics using these techniques is challe作者: 粗糙濫制 時(shí)間: 2025-3-29 16:59
Approaches for Determining DNA Persistence Length Using Atomic Force Microscopynical properties of DNA polymers depend on electrostatics and the strength of DNA base stacking by studying double-stranded DNA molecules incorporating several different neutral and charged base modifications. Here, we describe ten complementary approaches for determining DNA persistence length by A作者: avenge 時(shí)間: 2025-3-29 21:15
Quantitation of DNA-Binding Affinity Using Tethered Particle Motiony of those methods have intrinsic flaws that influence the outcome of the characterization. Tethered Particle Motion (TPM) is a simple, cheap, and high-throughput single-molecule method that can be used to reliably measure binding constants of proteins binding to DNA, provided that they distort DNA.作者: 辯論 時(shí)間: 2025-3-30 02:56
Observing Bacterial Chromatin Protein-DNA Interactions by Combining DNA Flow-Stretching with Single- chapter, we describe how the interaction between nucleoid-associated proteins and flow-stretched DNAs can be visualized on the single-molecule level. We describe three different experimental schemes that allow one to directly observe how these proteins that make up bacterial chromatin, associate wi作者: Morose 時(shí)間: 2025-3-30 07:39 作者: 可耕種 時(shí)間: 2025-3-30 10:06
Unraveling DNA Organization with Single-Molecule Force Spectroscopy Using Magnetic Tweezersity to specific genes for proper functioning of their hosts. The study of the structure and dynamics of the proteins that organize the genome has benefited tremendously from the development of single-molecule force spectroscopy techniques that allow for real-time, nanometer accuracy measurements of 作者: 神經(jīng) 時(shí)間: 2025-3-30 15:32
https://doi.org/10.1007/978-1-4939-8675-0nucleoprotein; genomic DNA; transcription; eukaryotic chromatin field; thermophoresis作者: neolith 時(shí)間: 2025-3-30 17:35
978-1-4939-9362-8Springer Science+Business Media, LLC, part of Springer Nature 2018作者: Demulcent 時(shí)間: 2025-3-30 22:33
Modular Assembly of Synthetic Secondary Chromosomesrch and biotechnological applications. Questions of how chromosomes need to be constructed to maintain the genetic information can now be answered by a learning-by-building approach. Here, we describe an efficient pipeline for the design and assembly of synthetic, secondary chromosomes in . based on the popular Modular Cloning system (MoClo).作者: thwart 時(shí)間: 2025-3-31 01:01
Bacterial Chromatin978-1-4939-8675-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 調(diào)味品 時(shí)間: 2025-3-31 08:55
Protein-Based Materials in Cosmeticsrch and biotechnological applications. Questions of how chromosomes need to be constructed to maintain the genetic information can now be answered by a learning-by-building approach. Here, we describe an efficient pipeline for the design and assembly of synthetic, secondary chromosomes in . based on the popular Modular Cloning system (MoClo).作者: 匯總 時(shí)間: 2025-3-31 10:16
