作者: 清洗 時間: 2025-3-21 22:46
Exon Trapping for Positional Cloning and Fingerprinting,ies, or phenotypes, to a quantitative trait locus (QTL), a genomic region no smaller than 1 centiMorgan (cM) or megabase (Mb) in length. Physical mapping can then provide a map of higher resolution. Physical maps are constructed from clones identified by screening genomic libraries. Genomic clones c作者: 初次登臺 時間: 2025-3-22 02:47
Isolation of CpG Islands From BAC Clones Using a Methyl-CpG Binding Column,ize, which are free of methylation and these are known as CpG islands (. . . and . for reviews). In addition to distinctive DNA characteristics, CpG islands have an open chromatin structure in that they are hyperacetylated, lack histone H1, and have a nucleosome-free region (.). The major reason for作者: 文件夾 時間: 2025-3-22 05:46
BAC Microarray-Based Comparative Genomic Hybridization,ridization (array CGH) provides a high throughput means to measure and map copy number aberrations on a genome-wide scale and to link them directly to genome sequence. For applications involving the analysis of tumors, the technology must provide reliable detection of single copy gains and losses in作者: conformity 時間: 2025-3-22 12:31
Large DNA Transformation in Plants,). Both methods used modified bacterial artificial chromosomes (BACs) such as BIBAC or pBACwich. BIBAC vector (used in tomato) is capable of transferring large DNA fragments from . into plants. pBACwich (used in tobacco) utilizes a Cre/. site-specific recombination system to integrate large DNA frag作者: moratorium 時間: 2025-3-22 15:09 作者: Amenable 時間: 2025-3-22 20:28
BAC Modification Using a RecA Expressing Shuttle Vector System,BACs have the capacity for maintaining large, often more than 300 kb fragments of mammalian DNA as stable inserts and avoid much of the insert chimerism which characterizes yeast artificial chromosomes (YACs). Isolation of BAC DNA is easy because they exist as supercoiled circular plasmids that are 作者: 做作 時間: 2025-3-23 01:17 作者: 自愛 時間: 2025-3-23 02:11 作者: 館長 時間: 2025-3-23 08:52
BAC Engineering for the Generation of ES Cell-Targeting Constructs and Mouse Transgenes,b), which can accommodate most eukaryotic genes along with their full set of regulatory elements, and to their greater convenience in handling over other large cloning vectors like P1-based artificial chromosomes (PACs) and yeast artificial chromosomes (YACs). Two key advances for harnessing the ful作者: decipher 時間: 2025-3-23 12:03
Microinjection of BAC DNA into the Pronuclei of Fertilized Mouse Oocytes,,.). The first successful attempt to perform this technique was carried out by Lin in 1966 (.), who could show that the early fertilized embryo could survive the mechanical damage of inserting a glass needle into the pronucleus. However, it was not until 1981 that small DNA fragments were integrated作者: 多產(chǎn)魚 時間: 2025-3-23 17:29
Generation of BAC Transgenic Mice,acterized “single” genes that contribute to a particular trait. However, single-gene expression in transgenic (.) mice is not appropriate for the functional analysis of the many genes that may reside in a typical genetically mapped locus of the order of 1 cM. Bacterial artificial chromosome (BAC) ve作者: GROVE 時間: 2025-3-23 19:56 作者: 妨礙議事 時間: 2025-3-24 00:11 作者: Monolithic 時間: 2025-3-24 03:40
,Cloning of β-Herpesvirus Genomes as Bacterial Artificial Chromosomes,have been completely sequenced, our knowledge on the function of the majority of herpesvirus genes is still quite insufficient. This is especially true for the members of the β-herpesviruses, i.e., the cytomegaloviruses (CMVs), because their slow replication kinetics, cell association, and large gen作者: AIL 時間: 2025-3-24 06:30
Construction of HSV-1 BACs and Their Use for Packaging of HSV-1-Based Amplicon Vectors,work of interactions between the virus and the host. Many of these interactions are still unrevealed or not understood in detail. The generation of mutant viruses is an important strategy to obtain insights into the functional role of individual virus genes. A classical genetic approach used chemica作者: Complement 時間: 2025-3-24 12:05 作者: 有權 時間: 2025-3-24 15:27 作者: 多余 時間: 2025-3-24 19:26
Herpesvirus Genome Mutagenesis by Transposon-Mediated Strategies,ruses are subclassified into .-, or .- herpesviruses, dependent upon a variety of criteria. Eight herpesviruses are human pathogens: Herpes Simplex Viruses type 1 and 2; Varicella-Zoster Virus (VZV); Cytomegalovirus (CMV); Human Herpes Viruses type 6 and 7; Epstein-Barr Virus (EBV); and Kaposi’s Sar作者: Inflated 時間: 2025-3-25 01:42 作者: CHAR 時間: 2025-3-25 06:22 作者: constitute 時間: 2025-3-25 08:55
Simon McGrath,Martin Mulder,Rebecca Suart have a mammalian selection marker or do not have one suitable for all cell types, it is usually necessary to modify BAC clones to include a selection marker, a “retrofitting” process. Different BAC retrofitting strategies have been developed over the last 5 yr and are classified in the reference section (.–.).作者: morale 時間: 2025-3-25 12:30
https://doi.org/10.1007/978-3-319-14346-0ion of genetic markers broadened to include blood group and histocompatibility antigens, and protein allotypes. Around 1980, DNA itself became the marker (.), first with restriction fragment length polymorphisms (RFLPs) and then with amplification polymorphisms based on simple sequence lengths (SSLP作者: barium-study 時間: 2025-3-25 17:02 作者: 使激動 時間: 2025-3-25 23:35
https://doi.org/10.1007/978-3-540-79567-4ize, which are free of methylation and these are known as CpG islands (. . . and . for reviews). In addition to distinctive DNA characteristics, CpG islands have an open chromatin structure in that they are hyperacetylated, lack histone H1, and have a nucleosome-free region (.). The major reason for作者: arboretum 時間: 2025-3-26 02:43
https://doi.org/10.1007/978-3-540-79567-4ridization (array CGH) provides a high throughput means to measure and map copy number aberrations on a genome-wide scale and to link them directly to genome sequence. For applications involving the analysis of tumors, the technology must provide reliable detection of single copy gains and losses in作者: condescend 時間: 2025-3-26 05:22 作者: STALL 時間: 2025-3-26 12:21
Simon McGrath,Martin Mulder,Rebecca Suart have a mammalian selection marker or do not have one suitable for all cell types, it is usually necessary to modify BAC clones to include a selection marker, a “retrofitting” process. Different BAC retrofitting strategies have been developed over the last 5 yr and are classified in the reference se作者: Cumbersome 時間: 2025-3-26 14:07 作者: exquisite 時間: 2025-3-26 18:28 作者: 廢墟 時間: 2025-3-26 22:17
Simon McGrath,Martin Mulder,Rebecca Suartventional cloning strategies, recombinogenic engineering can be carried out at virtually any chosen position on a given target DNA molecule (which can be small or large). Any type of modification, including sequence deletions, substitutions, and insertions can be carried out via homologous recombina作者: EVICT 時間: 2025-3-27 02:33 作者: 逢迎白雪 時間: 2025-3-27 06:49
https://doi.org/10.1007/978-3-319-08825-9,.). The first successful attempt to perform this technique was carried out by Lin in 1966 (.), who could show that the early fertilized embryo could survive the mechanical damage of inserting a glass needle into the pronucleus. However, it was not until 1981 that small DNA fragments were integrated作者: 感激小女 時間: 2025-3-27 10:35
Handbooks in Health, Work, and Disabilityacterized “single” genes that contribute to a particular trait. However, single-gene expression in transgenic (.) mice is not appropriate for the functional analysis of the many genes that may reside in a typical genetically mapped locus of the order of 1 cM. Bacterial artificial chromosome (BAC) ve作者: AMEND 時間: 2025-3-27 17:26
Hamidi Abdul Aziz,Salem S. Abu Amroducing a gene of interest into the genome and ectopically expressing it using a heterologous promoter. The choice of the heterologous promoter depends upon the tissue or the time at which a particular gene has to be expressed. Although these transgenic mice have been very informative, quite commonl作者: 即席演說 時間: 2025-3-27 17:56 作者: 責任 時間: 2025-3-28 01:35
Jessica A. Deaver,Sudeep C. Popathave been completely sequenced, our knowledge on the function of the majority of herpesvirus genes is still quite insufficient. This is especially true for the members of the β-herpesviruses, i.e., the cytomegaloviruses (CMVs), because their slow replication kinetics, cell association, and large gen作者: 返老還童 時間: 2025-3-28 03:06 作者: 蚊帳 時間: 2025-3-28 10:21
András Bárdossy,Abbas El Hachem artificial chromosomes (BACs) and their subsequent transfer into .. Owing to the high frequency of restriction sites, classical cloning methods for site-directed mutagenesis are not applicable to these large viral BACs. One possibility for mutagenesis is allele replacement by a two-step recombinati作者: critique 時間: 2025-3-28 13:12 作者: ARM 時間: 2025-3-28 17:53
Maurits W. Ertsen,Ruth A. Morganruses are subclassified into .-, or .- herpesviruses, dependent upon a variety of criteria. Eight herpesviruses are human pathogens: Herpes Simplex Viruses type 1 and 2; Varicella-Zoster Virus (VZV); Cytomegalovirus (CMV); Human Herpes Viruses type 6 and 7; Epstein-Barr Virus (EBV); and Kaposi’s Sar作者: Felicitous 時間: 2025-3-28 19:42 作者: Crater 時間: 2025-3-29 00:08 作者: NAV 時間: 2025-3-29 05:04 作者: 問到了燒瓶 時間: 2025-3-29 08:46 作者: GILD 時間: 2025-3-29 14:57 作者: enhance 時間: 2025-3-29 19:11 作者: 突變 時間: 2025-3-29 23:10
Isolation of CpG Islands From BAC Clones Using a Methyl-CpG Binding Column, the number of CpG islands in the human genome was estimated to be 34,200 (4 as modified by 7) and this figure is reasonably close to the 28,890 potential CpG islands that have been identified so far in the draft human genomic sequence (.).作者: REIGN 時間: 2025-3-30 02:52
BAC Modification Using a RecA Expressing Shuttle Vector System,tion markers necessary for easeful transfection study in mammalian cells. To use such BAC clones for transfection analysis, it is usually necessary to retrofit selectable marker gene cassettes, which will both allow the positive selection of the transfectant and facilitate the stable maintenance of 作者: 突襲 時間: 2025-3-30 04:51 作者: enterprise 時間: 2025-3-30 11:19
Microinjection of BAC DNA into the Pronuclei of Fertilized Mouse Oocytes,nsively comparing these applications. Although the basic technique for microinjection of large constructs are similar to those used for shorter DNA segments, there are some special requirements (.). In this chapter, we describe relevant steps for microinjection using large DNA constructs.作者: ALLAY 時間: 2025-3-30 16:05 作者: circuit 時間: 2025-3-30 18:01 作者: 保全 時間: 2025-3-30 21:08
Construction of HSV-1 BACs and Their Use for Packaging of HSV-1-Based Amplicon Vectors,tial for HSV-1 replication can only be produced on complementing cell lines (.). A more-efficient strategy for mutating HSV-1 was achieved by cloning the entire virus genome as a set of overlapping clones in cosmids. This allows the manipulation of specific virus genes on individual cosmid clones in作者: FISC 時間: 2025-3-31 03:30 作者: GLIB 時間: 2025-3-31 08:03 作者: HAWK 時間: 2025-3-31 09:26
https://doi.org/10.1007/978-3-540-79567-4 the number of CpG islands in the human genome was estimated to be 34,200 (4 as modified by 7) and this figure is reasonably close to the 28,890 potential CpG islands that have been identified so far in the draft human genomic sequence (.).作者: 虛假 時間: 2025-3-31 14:53 作者: Outmoded 時間: 2025-3-31 21:00
Kathija Yassim,Neville Rudman,Lucky Maluleke to ensure the stability of the insert. However, this hinders manipulation of BACs by homologous recombination in the bacterial cells. Consequently, the gene(s) required for recombination is transiently expressed to facilitate homologous recombination. Several different systems have been developed f作者: Nonporous 時間: 2025-3-31 22:16
https://doi.org/10.1007/978-3-319-08825-9nsively comparing these applications. Although the basic technique for microinjection of large constructs are similar to those used for shorter DNA segments, there are some special requirements (.). In this chapter, we describe relevant steps for microinjection using large DNA constructs.作者: Outwit 時間: 2025-4-1 05:17
Hamidi Abdul Aziz,Salem S. Abu Amrsence of the .-acting elements cannot be determined merely by the DNA sequence information. Bacterial artificial chromosomes (BACs) contain large segments of genomic DNA (>300 kb) and thus, most likely, contain all the .-regulatory elements of a gene (.). This large insert size provides an opportuni作者: anesthesia 時間: 2025-4-1 09:41
Jessica A. Deaver,Sudeep C. Popatntitious deletions, rearrangements, and illegitimate recombination events in the viral genomes have frequently been observed. It is especially cumbersome that the verification of the mutant genome structure can only be done at the very end of the lengthy isolation procedures of the mutant virus. Gen作者: Jubilation 時間: 2025-4-1 11:23 作者: Recessive 時間: 2025-4-1 15:32
András Bárdossy,Abbas El Hachemative for . and . because they are slightly more efficient for double crossing-over. In addition to .α/. and .β/., expression of the exonuclease inhibitor .γ . is necessary to allow mutagenesis in bacteria because the gam protein inhibits bacterial exonucleases and protects the linear recombination 作者: –LOUS 時間: 2025-4-1 21:29
