作者: Rustproof 時間: 2025-3-21 23:30 作者: Cupping 時間: 2025-3-22 02:03
Preparation of BAC Libraries From Bacterial Genomes by In Vitro Packaging,ery low frequency. On the other hand, in vitro packaging limits the size of the DNA based on the phage head stability. However, in vitro package is the most efficient method for introducing large-insert clones.作者: crescendo 時間: 2025-3-22 04:49
BAC Finishing Strategies,PCI-13 from P. de Jong (.) were fingerprinted, and the resulting maps of these overlapping clones have been integrated with clone maps of individual chromosomes generated by other centers (.). Sets of minimal overlapping clones have been selected for sequencing from the resulting maps.作者: 自制 時間: 2025-3-22 08:52 作者: Projection 時間: 2025-3-22 13:27
George J. Vachtsevanos,Kimon P. Valavanisse it relies on characterization of random clones in libraries comprising YACs or BACs. Even if a YAC or BAC contains an entire gene, the specific recovery of the gene was an arduous process. Often a gene is available as a contiguous set of fragments that must be pieced together.作者: Ebct207 時間: 2025-3-22 20:27
Sexual Minority Groups and Urban Healthe found that templates produced through RCA yield more consistent and higher-quality sequence than identical templates generated from plasmid-prep methods. The protocol is simple, amenable to high throughput, and currently in use at the DOE Joint Genome Institute (Walnut Creek, CA) for the daily production of 30,000 sequencing templates.作者: 連累 時間: 2025-3-22 22:50
Tree Roots versus Sidewalks and Sewers,e the sequences contained in them. Thus, both physical and functional mapping objectives would be facilitated by an efficient strategy for making nested deletions from one end of the insert DNA in a BAC or PAC clone.作者: NEX 時間: 2025-3-23 02:59 作者: hidebound 時間: 2025-3-23 07:53
Rolling Circle Amplification for Sequencing Templates,e found that templates produced through RCA yield more consistent and higher-quality sequence than identical templates generated from plasmid-prep methods. The protocol is simple, amenable to high throughput, and currently in use at the DOE Joint Genome Institute (Walnut Creek, CA) for the daily production of 30,000 sequencing templates.作者: CANON 時間: 2025-3-23 11:08 作者: dainty 時間: 2025-3-23 15:44 作者: 針葉樹 時間: 2025-3-23 19:37 作者: 六邊形 時間: 2025-3-23 22:43
1064-3745 niques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs. Volume 1 presents readily reproducible techniques for library construction, physical mapping, and sequencing.. An accompanying volume, Volume 2: Functional Studies作者: Intact 時間: 2025-3-24 04:36
Xiangguosi Gas Storage Design and Operation,syphilis-causing spirochete, ., genetic studies are highly limited because the bacterium cannot be continuously grown in the laboratory, because of its high virulence and a total lack of genetic tools to study this organism.作者: 客觀 時間: 2025-3-24 10:28 作者: Intend 時間: 2025-3-24 11:04
Mark R. Montgomery,Alex C. Ezehalternative chemistry sequencing, polymerase chain reaction (PCR), as well as other standard tools for acquiring high-quality data in low-coverage or low-quality regions. In addition to BAC subclones, transposon-mediated sequencing can be applied to cDNA or PCR products subcloned into vectors.作者: 高度 時間: 2025-3-24 18:13
Construction of Small Genome BAC Library for Functional and Genomic Applications,syphilis-causing spirochete, ., genetic studies are highly limited because the bacterium cannot be continuously grown in the laboratory, because of its high virulence and a total lack of genetic tools to study this organism.作者: 聚集 時間: 2025-3-24 22:26 作者: FLAG 時間: 2025-3-24 23:33 作者: 稱贊 時間: 2025-3-25 07:14
Kimon P. Valavanis,George J. Vachtsevanosject drawing near (.), the genome-sequencing community is now directing its efforts at sequencing commercially important organisms. BAC library construction and high-throughput screening has become an indispensable tool for the isolation of large chromosomal DNA fragments necessary for such projects.作者: jealousy 時間: 2025-3-25 07:53
A Systematic Examination of the Handthat are present at multiple other points in the genome. Therefore, ideally, the same templates should be used as an integrating framework of entry points for sequencing and then applied to gene isolation and mapping, studies of genome organization and evolution, and a myriad of clinical applications (.).作者: deviate 時間: 2025-3-25 12:39 作者: Constituent 時間: 2025-3-25 19:24 作者: conference 時間: 2025-3-25 23:26
Hybridization-Based Selection of BAC Clones,ject drawing near (.), the genome-sequencing community is now directing its efforts at sequencing commercially important organisms. BAC library construction and high-throughput screening has become an indispensable tool for the isolation of large chromosomal DNA fragments necessary for such projects.作者: pacific 時間: 2025-3-26 00:51 作者: 破譯 時間: 2025-3-26 06:54 作者: MAZE 時間: 2025-3-26 11:13 作者: corpuscle 時間: 2025-3-26 13:26 作者: 災(zāi)禍 時間: 2025-3-26 20:08
Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/180254.jpg作者: collagenase 時間: 2025-3-26 21:32
Bacterial Artificial Chromosomes978-1-59259-752-9Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 現(xiàn)存 時間: 2025-3-27 04:39
https://doi.org/10.1385/1592597521BAC; Chromosom; DNA; DNA sequencing; PAC; hybridization; reproducible techniques作者: 歡樂中國 時間: 2025-3-27 06:35 作者: Substance-Abuse 時間: 2025-3-27 12:54 作者: mechanism 時間: 2025-3-27 14:05 作者: Adulate 時間: 2025-3-27 19:19 作者: 無思維能力 時間: 2025-3-28 01:55
Caitlin Powers,Daniel Mellinger,Vijay Kumar DNA from different species. These BAC libraries are constructed by insertion of DNA fragments from different species into a vector, which can be replicated in a bacterial host. Choosing . as the model host has many advantages: rapid growth of the host, high stability of the DNA fragment when inside作者: hermitage 時間: 2025-3-28 03:21 作者: follicular-unit 時間: 2025-3-28 09:07
A Systematic Examination of the Handonventional methods to screen large-insert clone libraries for specific clones within a defined chromosomal interval are polymerase chain reaction (PCR) based using microsatellite markers. This strategy is labor- and cost-intensive and requires the PCR amplification of several thousands of DNA sampl作者: Cardiac-Output 時間: 2025-3-28 13:21
A Systematic Examination of the Handrstanding disease, development and evolution. To accomplish this goal and a broad spectrum of applications requires integration of genome sequence information to genetic markers (expressed sequence tag/cDNA/gene transcripts content) and to reagents that can be seen through a microscope and linked to作者: conformity 時間: 2025-3-28 16:37
Anatomy and Function of the Hand et al. (.) for use in the construction of high-resolution physical maps from low-copynumber, large-insert clones. The procedure is robust and allows for the recovery of clone insert size information. Initially used to construct sequence tag site (STS)-based contigs (.), the methodology has also bee作者: prediabetes 時間: 2025-3-28 20:39 作者: Chagrin 時間: 2025-3-28 23:39 作者: 消息靈通 時間: 2025-3-29 07:00
Handbook of Upper Extremity Examinationr fragments; end sequencing these smaller fragments; and from the overlapping sequences of the randomly generated fragments, reassembling the initial target sequence. Although this random strategy initially was described more than two decades ago (.–.), only a few years following the original report作者: Albinism 時間: 2025-3-29 08:21
Sexual Minority Groups and Urban Healthn have been limited to purification of biologically propagated M13 or plasmid-based templates, or in vitro amplification of such templates by polymerase chain reaction (PCR). In this chapter, we describe a protocol for a new approach to template generation: rolling circle amplification (RCA). We hav作者: Loathe 時間: 2025-3-29 14:55
Mark R. Montgomery,Alex C. Ezeh stages of bacterial artificial chromosome (BAC) sequencing, allowing the user to expand a finishing repertoire of custom oligonucleotide sequencing, alternative chemistry sequencing, polymerase chain reaction (PCR), as well as other standard tools for acquiring high-quality data in low-coverage or 作者: Optimum 時間: 2025-3-29 18:16 作者: 盲信者 時間: 2025-3-29 23:43
Tree Inventory and Systematic Management,in a typical amplification, the primers should hybridize only to the target sequence. However, there is a relatively narrow range of conditions (temperature, concentrations of ions and denaturing agents) for specific annealing of an oligonucleotide to its complementary target, and often it does not 作者: Addictive 時間: 2025-3-30 01:30
Tree Roots versus Sidewalks and Sewers, methods for obtaining position-specific sequence information along the insert DNA. Because substantial quantities of high-quality clone DNA can be easily prepared from BACs and PACs, they are also potentially valuable reagents for direct use in biologic experiments that seek to functionally evaluat作者: TIA742 時間: 2025-3-30 07:05
Interpersonally Comparable Utility,n a BAC or P1 vector have been used to sequence either end of a genomic insert to facilitate selection of minimally overlapping clones for analyzing large chromosomal regions (.). The entire insert in a BAC or P1-derived artificial chromosome (PAC) clone, however, is usually sequenced using shotgun 作者: 灌輸 時間: 2025-3-30 11:29
https://doi.org/10.1007/978-1-4020-7964-1s. The approach chosen by the International Human Genome Sequencing Consortium is a hierarchical mapping and sequencing strategy similar to that described for other large genomes, including ., and . (.–.). A physical map of the human genome has been assembled on the basis of “fingerprints” generated作者: echnic 時間: 2025-3-30 15:13 作者: FANG 時間: 2025-3-30 17:53
Construction of Small Genome BAC Library for Functional and Genomic Applications,nic; and, second, a broad spectrum of genetic techniques for the particular bacteria being studied must be available. For other bacteria, such as the syphilis-causing spirochete, ., genetic studies are highly limited because the bacterium cannot be continuously grown in the laboratory, because of it作者: Chipmunk 時間: 2025-3-30 20:41 作者: precede 時間: 2025-3-31 02:05
Exploring Transformation-Associated Recombination Cloning for Selective Isolation of Genomic RegionBAC) cloning systems (.,.). These techniques have made the isolation of large, random DNA fragments possible, thereby greatly simplifying the physical mapping of chromosomes. However, isolation of entire genes and specific chromosomal regions for functional studies remained a laborious process becau作者: Coronary-Spasm 時間: 2025-3-31 06:03
Purification of BAC DNA, DNA from different species. These BAC libraries are constructed by insertion of DNA fragments from different species into a vector, which can be replicated in a bacterial host. Choosing . as the model host has many advantages: rapid growth of the host, high stability of the DNA fragment when inside作者: 痛恨 時間: 2025-3-31 10:17
Hybridization-Based Selection of BAC Clones,derived from target genomic regions is essential for detailed analysis such as sequencing. Since its introduction in 1992, the bacterial artificial chromosome (BAC) library system has been widely employed as a standard cloning system for mapping and sequencing the genomes of human and model organism作者: 漂白 時間: 2025-3-31 13:51 作者: Postmenopause 時間: 2025-3-31 17:36
BAC Mapping Using Fluorescence , Hybridization,rstanding disease, development and evolution. To accomplish this goal and a broad spectrum of applications requires integration of genome sequence information to genetic markers (expressed sequence tag/cDNA/gene transcripts content) and to reagents that can be seen through a microscope and linked to作者: ostrish 時間: 2025-3-31 23:56 作者: tangle 時間: 2025-4-1 04:29
