標(biāo)題: Titlebook: BCL-2 Family Proteins; Methods and Protocol Evripidis Gavathiotis Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2 [打印本頁] 作者: Coolidge 時(shí)間: 2025-3-21 19:39
書目名稱BCL-2 Family Proteins影響因子(影響力)
書目名稱BCL-2 Family Proteins影響因子(影響力)學(xué)科排名
書目名稱BCL-2 Family Proteins網(wǎng)絡(luò)公開度
書目名稱BCL-2 Family Proteins網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱BCL-2 Family Proteins被引頻次
書目名稱BCL-2 Family Proteins被引頻次學(xué)科排名
書目名稱BCL-2 Family Proteins年度引用
書目名稱BCL-2 Family Proteins年度引用學(xué)科排名
書目名稱BCL-2 Family Proteins讀者反饋
書目名稱BCL-2 Family Proteins讀者反饋學(xué)科排名
作者: Mucosa 時(shí)間: 2025-3-21 22:28
Liposomal Permeabilization Assay to Study the Functional Interactions of the BCL-2 Family, be quantified by the increase in fluorescence intensity as the fluorophore and quencher dissociate. The liposomal permeabilization assay has been used to delineate interactions among BCL-2 family members as well as to characterize peptides, small molecules, and lipids that modulate the function of 作者: 確保 時(shí)間: 2025-3-22 01:16
Reconstitution and Characterization of BCL-2 Family Proteins in Lipid Bilayer Nanodiscs, a dominant inhibitor of programmed cell death and a major anticancer drug target. The protocols are relatively straightforward. Provided care is taken to ensure protein integrity and sample homogeneity, BCL-XL can be readily reconstituted in nanodiscs, with its hydrophobic C-terminal tail anchored 作者: judiciousness 時(shí)間: 2025-3-22 06:42
1064-3745 essary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and thorough, .BCL-2 978-1-4939-8861-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 膽小懦夫 時(shí)間: 2025-3-22 10:59 作者: 興奮過度 時(shí)間: 2025-3-22 15:32
Heather Winskel,Theeraporn Ratitamkul be quantified by the increase in fluorescence intensity as the fluorophore and quencher dissociate. The liposomal permeabilization assay has been used to delineate interactions among BCL-2 family members as well as to characterize peptides, small molecules, and lipids that modulate the function of 作者: Amenable 時(shí)間: 2025-3-22 19:19 作者: 使人入神 時(shí)間: 2025-3-22 23:15 作者: eucalyptus 時(shí)間: 2025-3-23 01:45
Liquid Metal Biomedical Imaging,ds. Finally, we describe how to generate MOMP-resistant cell lines, using CRISPR-Cas9 mediated deletion of BAX and BAK. Facilitating the investigation of mitochondrial apoptosis, mito-priming provides a clean, robust way to induce mitochondrial apoptosis both in vitro and in vivo.作者: rheumatism 時(shí)間: 2025-3-23 06:03
Application of Mito-Priming to Generate BCL-2 Addicted Cells,ds. Finally, we describe how to generate MOMP-resistant cell lines, using CRISPR-Cas9 mediated deletion of BAX and BAK. Facilitating the investigation of mitochondrial apoptosis, mito-priming provides a clean, robust way to induce mitochondrial apoptosis both in vitro and in vivo.作者: Chronic 時(shí)間: 2025-3-23 11:57 作者: opprobrious 時(shí)間: 2025-3-23 17:05
Liquid Metal Chemical Reaction,versus survival, leading to the development of BH3 mimetics as therapeutics. Here we provide an overview of BCL-2 family interactions, their relevance in health and disease, and the progress toward regulating their interactions therapeutically.作者: narcotic 時(shí)間: 2025-3-23 21:41
Heather Winskel,Theeraporn Ratitamkulf Bcl-2 proteins with photoreactive amino acid analogs incorporated at specific locations. These photoreactive proteins are reconstituted into liposomal membranes with defined phospholipids or mitochondrial membranes isolated from animals, and their interactions with other Bcl-2 proteins are detected by photocrosslinking.作者: 溫和女孩 時(shí)間: 2025-3-24 00:40 作者: 擁擠前 時(shí)間: 2025-3-24 05:23 作者: 消音器 時(shí)間: 2025-3-24 08:43 作者: 統(tǒng)治人類 時(shí)間: 2025-3-24 13:21
Lithium in the Acute Treatment of Maniaystems—outer membrane vesicles (OMVs) and liposomes—to visualize the pores in the membrane by using cryo-electron microscopy (cryo-EM). We have revealed the morphology of the pore, determined the localization of Bax labeled with nanogold and have performed image analysis to help understand the mechanisms of pore formation induced by Bax.作者: 昏睡中 時(shí)間: 2025-3-24 17:29 作者: enflame 時(shí)間: 2025-3-24 19:28 作者: paleolithic 時(shí)間: 2025-3-25 00:21 作者: Sinus-Node 時(shí)間: 2025-3-25 04:20
Leo Man-Lit Cheang,Catherine McBridectivation and apoptotic cell death. To generate pores, BAK and BAX undergo major changes including BAX translocation to the outer membrane, and partial unfolding, dimerization, and oligomerization. Here we describe biochemical protocols that can be used on most cell types to gain a population overview of BAK and BAX status.作者: Initiative 時(shí)間: 2025-3-25 08:18 作者: 半身雕像 時(shí)間: 2025-3-25 14:50 作者: 倔強(qiáng)不能 時(shí)間: 2025-3-25 16:32
Probing BAK and BAX Activation and Pore Assembly with Cytochrome , Release, Limited Proteolysis, anctivation and apoptotic cell death. To generate pores, BAK and BAX undergo major changes including BAX translocation to the outer membrane, and partial unfolding, dimerization, and oligomerization. Here we describe biochemical protocols that can be used on most cell types to gain a population overview of BAK and BAX status.作者: Yag-Capsulotomy 時(shí)間: 2025-3-25 23:00 作者: Fracture 時(shí)間: 2025-3-26 02:40 作者: 抗生素 時(shí)間: 2025-3-26 08:06 作者: Chivalrous 時(shí)間: 2025-3-26 09:31
Assessment of Dynamic BCL-2 Protein Shuttling Between Outer Mitochondrial Membrane and Cytosol,nd shows correlation between relative BAX and BAK localizations and cellular predisposition to apoptosis. The retrotranslocation of BCL-2 proteins from the OMM can be measured using fluorescence-labeled protein in intact cells or endogenous protein from isolated heavy membrane fractions.作者: 輕浮女 時(shí)間: 2025-3-26 13:28
Quantification of BCL-2 Family Members by Flow Cytometry,blotting. Here we describe a robust and reproducible method to measure the expression levels of the BCL-2 family members, BCL-2, BCL-X., and MCL-1 by quantitative flow cytometry (QFCM) using validated antibodies.作者: 因無茶而冷淡 時(shí)間: 2025-3-26 16:51
Methods to Probe Conformational Activation and Mitochondrial Activity of Proapoptotic BAK,apoptosis, BAK undergoes conformational changes to form the active species associated with apoptotic pores. We describe methods to purify mitochondria for MOMP assays and to detect conformational changes in native BAK associated with MOMP by using limited proteolysis and cross-linking analyses.作者: 癡呆 時(shí)間: 2025-3-26 21:12
Cryo-Electron Microscopy to Study Bax Pores and MOMP,ystems—outer membrane vesicles (OMVs) and liposomes—to visualize the pores in the membrane by using cryo-electron microscopy (cryo-EM). We have revealed the morphology of the pore, determined the localization of Bax labeled with nanogold and have performed image analysis to help understand the mechanisms of pore formation induced by Bax.作者: 跟隨 時(shí)間: 2025-3-27 05:00
Rapid Imaging of BCL-2 Family Interactions in Live Cells Using FLIM-FRET,al data acquisition. We developed an automated optical system with eight parallel detectors for rapid and efficient data collection. Here we describe how to use this system for FLIM-FRET imaging of Bcl-2 family protein interactions in a 384-well plate format.作者: AV-node 時(shí)間: 2025-3-27 09:02 作者: 表狀態(tài) 時(shí)間: 2025-3-27 13:31 作者: MAG 時(shí)間: 2025-3-27 14:27 作者: Headstrong 時(shí)間: 2025-3-27 19:27 作者: amplitude 時(shí)間: 2025-3-28 00:27
Overview of BCL-2 Family Proteins and Therapeutic Potentials,ein family, both literally and figuratively. BH3 motifs are present in antiapoptotic and proapoptotic BCL-2 homologs, and in a separate group of unrelated BH3-only proteins often appended to the BCL-2 family. BH3-containing helices mediate many of their physical interactions to determine cell death 作者: peritonitis 時(shí)間: 2025-3-28 02:14 作者: 匍匐前進(jìn) 時(shí)間: 2025-3-28 09:08
Application of Mito-Priming to Generate BCL-2 Addicted Cells,gement of mitochondrial outer membrane permeabilization or MOMP to initiate cell death. We have developed a new method, called mito-priming, that allows for rapid and synchronous induction of mitochondrial apoptosis in an on-target manner. Mito-priming uses coexpression of pro- and antiapoptotic Bcl作者: TRACE 時(shí)間: 2025-3-28 11:59 作者: 招募 時(shí)間: 2025-3-28 14:49
Flow Cytometry-Based Detection and Analysis of BCL-2 Family Proteins and Mitochondrial Outer Membraderstanding of the mechanistic interactions between these proteins continues to evolve in normal and malignant cells. The functional variation by individual BCL-2 proteins in different cell types has driven clinical therapeutic development in targeting individual BCL-2 members with the goal of fine-作者: Minatory 時(shí)間: 2025-3-28 22:25
Investigating BCL-2 Family Protein Interactions in Yeast, yeast . may look out of place. However, when grown under adequate conditions, yeast cells have mitochondria that have similar properties as those of mammalian cells, and are able to be targeted by mammalian Bcl-2 family members. Yeast thus provides a neutral cellular background to study how protein作者: 斗志 時(shí)間: 2025-3-28 22:56
Liposomal Permeabilization Assay to Study the Functional Interactions of the BCL-2 Family,y BCL-2 family members have been classified in three groups based on structural homology and function. The multidomain antiapoptotic proteins promote survival, whereas the multidomain and the BH3-only proapoptotic members induce cell death. Because the interaction among the BCL-2 family members occu作者: 革新 時(shí)間: 2025-3-29 04:44 作者: 發(fā)芽 時(shí)間: 2025-3-29 10:07 作者: defray 時(shí)間: 2025-3-29 13:35
Assessment of Dynamic BCL-2 Protein Shuttling Between Outer Mitochondrial Membrane and Cytosol,nd BAK but also prosurvival family members, like BCL-x. or MCL-1, translocate to the outer mitochondrial membrane (OMM) and retrotranslocate from the mitochondria back into the cytosol. The resulting equilibrium produces a broad range of localization pattern observed for BAX and BAK in human cells a作者: GREG 時(shí)間: 2025-3-29 15:38
Quantification of BCL-2 Family Members by Flow Cytometry,ure the expression levels of specific proteins in a cell population of interest without the need to physically separate out the cells from within a heterogeneous population by using the appropriate cell-specific markers. It also requires fewer cells than other traditional techniques such as Western 作者: 事情 時(shí)間: 2025-3-29 23:13
Methods to Probe Calcium Regulation by BCL-2 Family Members,ortant function is the release of calcium from the endoplasmic reticulum (ER), which critically contributes to the process of apoptosis. Here, we describe a protocol to measure calcium levels in the ER, mitochondria, and cytosol, with specific consideration of BCL-2 family biology.作者: colony 時(shí)間: 2025-3-30 00:51 作者: 背書 時(shí)間: 2025-3-30 06:22
Probing BAK and BAX Activation and Pore Assembly with Cytochrome , Release, Limited Proteolysis, an These two proteins generate pores in the mitochondrial outer membrane that release factors such as cytochrome . into the cytosol to trigger caspase activation and apoptotic cell death. To generate pores, BAK and BAX undergo major changes including BAX translocation to the outer membrane, and partia作者: hemophilia 時(shí)間: 2025-3-30 11:18
BCL-2 Protein Family Interaction Analysis by Nuclear Magnetic Resonance Spectroscopy,ll molecule binding. NMR has been used extensively in the investigation of BCL-2 family proteins revealing the structure of key family members, identifying binding partners and interaction sites, and screening small molecule modulators. In this chapter we discuss the application of NMR to identify i作者: foliage 時(shí)間: 2025-3-30 14:11
Reconstitution and Characterization of BCL-2 Family Proteins in Lipid Bilayer Nanodiscs,drugs. Because their functions are intimately connected with intracellular membranes, it is important to perform structural and activity studies in precisely characterized samples that include phospholipids and capture the features of the native physiological environment as closely as possible. NMR 作者: 災(zāi)難 時(shí)間: 2025-3-30 20:32 作者: 乞丐 時(shí)間: 2025-3-30 23:19 作者: PRO 時(shí)間: 2025-3-31 04:54
Rapid Imaging of BCL-2 Family Interactions in Live Cells Using FLIM-FRET,to detect F?rster resonance energy transfer (FRET) between two fluorescent-fusion proteins in live cells. FLIM-FRET has been used to detect specific interactions and their disruption, for Bcl-2 family proteins. To date, this has been possible only in low throughput, due to the time required for seri作者: 無可爭辯 時(shí)間: 2025-3-31 05:32
Mitochondrial Isolation and Real-Time Monitoring of MOMP,subset of MOMP regulators. Here we describe the method to isolate, JC-1-label, and purify mouse liver mitochondria and subsequently describe how to utilize the JC-1-labeled mitochondria for real-time MOMP measurements.作者: myriad 時(shí)間: 2025-3-31 09:36
Methods to Probe Calcium Regulation by BCL-2 Family Members,ortant function is the release of calcium from the endoplasmic reticulum (ER), which critically contributes to the process of apoptosis. Here, we describe a protocol to measure calcium levels in the ER, mitochondria, and cytosol, with specific consideration of BCL-2 family biology.作者: Archipelago 時(shí)間: 2025-3-31 14:56 作者: Urea508 時(shí)間: 2025-3-31 20:51 作者: Insul島 時(shí)間: 2025-4-1 00:50 作者: 密碼 時(shí)間: 2025-4-1 02:59
Liquid Metal Chemical Reaction,ein family, both literally and figuratively. BH3 motifs are present in antiapoptotic and proapoptotic BCL-2 homologs, and in a separate group of unrelated BH3-only proteins often appended to the BCL-2 family. BH3-containing helices mediate many of their physical interactions to determine cell death
