作者: 圣人 時(shí)間: 2025-3-21 21:21
Fingerprint Analysis and Representation,me a highly feasible and useful addition to any immunology research program. In this review, we discuss the technical aspects of the procedure, as well as its advantages and drawbacks compared to alternative approaches, and our practical experience in establishing the system at the University of Rochester.作者: Intrepid 時(shí)間: 2025-3-22 03:53 作者: BROOK 時(shí)間: 2025-3-22 05:09 作者: BOOR 時(shí)間: 2025-3-22 09:57 作者: 車(chē)床 時(shí)間: 2025-3-22 16:29
https://doi.org/10.1007/978-3-319-76526-6mice expressing HEL as a systemic, intracellular, membrane-bound self-antigen. The same principles and methods apply to immunoglobulin transgenes. We briefly discuss the techniques that could be used to explore mechanisms of tolerance to systemic intracellular antigens in these mice.作者: amnesia 時(shí)間: 2025-3-22 17:17 作者: 虛構(gòu)的東西 時(shí)間: 2025-3-23 01:06
Analysis of Lymphocyte Development and Function Using the RAG-Deficient Blastocyst Complementation me a highly feasible and useful addition to any immunology research program. In this review, we discuss the technical aspects of the procedure, as well as its advantages and drawbacks compared to alternative approaches, and our practical experience in establishing the system at the University of Rochester.作者: 內(nèi)閣 時(shí)間: 2025-3-23 04:26 作者: GLUE 時(shí)間: 2025-3-23 06:06
Immunoglobulin Class Switching,ass switch recombination. Cytokine signals can direct class switch recombination to distinct classes; for example, interleukin-4 will target murine IgG1 and IgE, and human IgG4 and IgE. Here we describe the technologies for the isolation of B lymphocytes, their activation to class switching, and the analysis of Ig class switching.作者: concubine 時(shí)間: 2025-3-23 10:58 作者: 憤慨一下 時(shí)間: 2025-3-23 17:44
Analysis of B-Cell Immune Tolerance Induction Using Transgenic Mice,mice expressing HEL as a systemic, intracellular, membrane-bound self-antigen. The same principles and methods apply to immunoglobulin transgenes. We briefly discuss the techniques that could be used to explore mechanisms of tolerance to systemic intracellular antigens in these mice.作者: 刻苦讀書(shū) 時(shí)間: 2025-3-23 21:40 作者: echnic 時(shí)間: 2025-3-24 02:16
Verifying Floating-Point Algorithmsid rafts from B lymphocytes is described. Particular emphasis is given to the potential artifacts inherent in current procedures that rely on detergents to isolate lipid rafts and alternative technologies that may circumvent these.作者: Cultivate 時(shí)間: 2025-3-24 03:57 作者: miracle 時(shí)間: 2025-3-24 06:49
Analysis of the Germinal Center Reaction and In Vivo Long-Lived Plasma Cells,rodissection. From these NP-specific recombined VDJ genes are amplified with family-specific primers by polymerase chain reaction (PCR) for deoxyribonucleic acid (DNA) sequencing. The methods described can be used to characterize origins and lifespan of plasma cells in vivo.作者: delegate 時(shí)間: 2025-3-24 12:33 作者: 發(fā)電機(jī) 時(shí)間: 2025-3-24 18:02 作者: Encoding 時(shí)間: 2025-3-24 21:36
Synthetic Fingerprint Generation,-cell stages. It also outlines assays for the characterization of these cells, including procedures for testing functional lineage restriction, determination of rearrangement status, analyses of gene expression at the ribonucleic acid (RNA) and protein level, and assessment of cell cycle state.作者: 繁榮中國(guó) 時(shí)間: 2025-3-25 02:08 作者: Keratectomy 時(shí)間: 2025-3-25 06:51
Davide Maltoni,Dario Maio,Salil Prabhakarf BrdU for short-term labeling experiments. Using the protocol described, it is possible to combine flow cytometric detection of incorporated BrdU simultaneously with fluorescence staining of various cell surface markers.作者: observatory 時(shí)間: 2025-3-25 10:21
1064-3745 iology and pathology of the immune system in higher animals. While recent advances in genetics and cellular and molecular biology have provided a large spectrum of powerful new experimental tools in this field, it is both time consuming and often very difficult for a student or just any bench-side w作者: 議程 時(shí)間: 2025-3-25 13:15
Fingerprint Analysis and Representation,e targeting, to in vitro manipulate embryonic stem (ES) cells and to rapidly identify successfully targeted ES colonies. It also provides an updated list of Cre strains currently used to assess gene function at defined stages of B-cell development and guidelines to generate single-copy, knock-in transgenes regulated in a Cre-dependent manner.作者: giggle 時(shí)間: 2025-3-25 18:18
Fingerprint Analysis and Representation,coding either normal or blocking forms of experimental proteins, into murine splenic B cells using retrovirus vectors. This makes it possible to study the effects of these proteins on late stages of B-cell development, including proliferation, class switch recombination, and plasmacytic differentiation.作者: 相容 時(shí)間: 2025-3-25 22:01
https://doi.org/10.1007/978-3-319-76526-6evelopment. Here the protocols for how to grow and to study the differentiation of mouse pre-B-1, as developed in our laboratory, are described. Moreover, the protocols of how to grow and to test the plasticity of .-deficient pre-B-1 cells are also outlined.作者: 統(tǒng)治人類(lèi) 時(shí)間: 2025-3-26 02:32 作者: ureter 時(shí)間: 2025-3-26 05:11
Introduction of Genes Into Primary Murine Splenic B Cells Using Retrovirus Vectors,coding either normal or blocking forms of experimental proteins, into murine splenic B cells using retrovirus vectors. This makes it possible to study the effects of these proteins on late stages of B-cell development, including proliferation, class switch recombination, and plasmacytic differentiation.作者: 輪流 時(shí)間: 2025-3-26 12:12 作者: receptors 時(shí)間: 2025-3-26 13:36 作者: BAIL 時(shí)間: 2025-3-26 18:53
Characterization of B-Cell Maturation in the Peripheral Immune System,ry, analyzing the relative expression of several developmentally regulated markers. We describe the method for the staining of the cells and the analysis of the collected data and show examples of the results obtained in normal and mutant mice.作者: Guileless 時(shí)間: 2025-3-26 22:18 作者: 節(jié)省 時(shí)間: 2025-3-27 04:25 作者: Neonatal 時(shí)間: 2025-3-27 06:47 作者: 圍裙 時(shí)間: 2025-3-27 10:56
Synthetic Fingerprint Generation, proteins that can serve as markers for recognizing distinct stages in this process. These cell fractions range from just prior to initial heavy chain rearrangement, the germline pro-B stage, through D-J rearranged pro-B and heavy chain expressing pre-B stages, to the maturing surface BCR positive B作者: GLOSS 時(shí)間: 2025-3-27 14:26
Fingerprint Analysis and Representation,are recent bone marrow emigrants traveling with the blood to the spleen. Here they further develop to transitional 2 and mature B cells. Marginal zone B cells are a sessile population only found in the spleen. The distinction of these cell types is only possible by three- and four-color flow cytomet作者: Cocker 時(shí)間: 2025-3-27 20:23
Synthetic Fingerprint Generation,e identification of additional subset markers and the extension of fluorescence-activated cell sorter (FACS) capabilities to simultaneously measure the expression of more markers on individual cells. For example, introduction of a 6–8 multiparameter FACS instrument resulted in significant advances i作者: Amendment 時(shí)間: 2025-3-27 22:49
Davide Maltoni,Dario Maio,Salil Prabhakarques employed, labeling of dividing cells with the thymidine analog 5-bromo-2′-deoxyuridine (BrdU) has turned out to be a versatile and reliable procedure. Labeling of the cells can be easily performed in vivo by feeding BrdU in the drinking water for extended periods of time or by an ip injection o作者: CLAIM 時(shí)間: 2025-3-28 04:57 作者: 委屈 時(shí)間: 2025-3-28 08:08 作者: Adrenal-Glands 時(shí)間: 2025-3-28 10:49
Fingerprint Analysis and Representation,e-specific, and inducible manner. This chapter provides a set of updated protocols that will help the reader to construct a vector for conditional gene targeting, to in vitro manipulate embryonic stem (ES) cells and to rapidly identify successfully targeted ES colonies. It also provides an updated l作者: lattice 時(shí)間: 2025-3-28 18:19
Synthetic Fingerprint Generation,NP) coupled to a T-cell-dependent carrier. Antigen-specific germinal center cells and extrafollicular plasma cells are identified by immunohistology on tissue sections. Plasma cells labeled with 5-bromo-2′-deoxyuridine (BrdU) pulses given during different phases of B-cell response are identified on 作者: 分期付款 時(shí)間: 2025-3-28 22:07 作者: semble 時(shí)間: 2025-3-29 01:50
Fingerprint Analysis and Representation, recombination and terminal differentiation to become immunoglobulin-secreting plasmacytic cells. Methods are described here for introducing genes, encoding either normal or blocking forms of experimental proteins, into murine splenic B cells using retrovirus vectors. This makes it possible to study作者: 沒(méi)花的是打擾 時(shí)間: 2025-3-29 06:42 作者: 肌肉 時(shí)間: 2025-3-29 07:35 作者: cochlea 時(shí)間: 2025-3-29 14:09 作者: RENAL 時(shí)間: 2025-3-29 17:57 作者: 少量 時(shí)間: 2025-3-29 20:57
Verifying Floating-Point Algorithmsgolipid- and cholesterol-rich membrane microdomains, termed ., which serve as platforms for both receptor signaling and trafficking. In B lymphocytes lipid rafts appear to play a key role in the initiation of B-cell antigen receptor (BCR) signaling. Current methods to isolate lipid rafts rely on the作者: 都相信我的話 時(shí)間: 2025-3-30 01:17
Verifying Floating-Point Algorithms for B-lineage cells. This chapter describes an approach to isolate single cells from frozen tissue sections by microdissection using a hydraulic micromanipulator and the subsequent amplification of rearranged IgH and Igκ genes from the cells in a seminested polymerase chain reaction (PCR) approach.作者: MIME 時(shí)間: 2025-3-30 04:54 作者: 優(yōu)雅 時(shí)間: 2025-3-30 08:49 作者: 散布 時(shí)間: 2025-3-30 15:38
https://doi.org/10.1007/978-3-319-76526-6which developmentally occur at different cellular stages. Various in vitro models have been very useful in unraveling this complex process of B-cell development. Here the protocols for how to grow and to study the differentiation of mouse pre-B-1, as developed in our laboratory, are described. Moreo作者: 謙卑 時(shí)間: 2025-3-30 18:47
Hua Gu,Klaus RajewskyIncludes supplementary material: 作者: Parabola 時(shí)間: 2025-3-30 23:44 作者: 油膏 時(shí)間: 2025-3-31 03:24
Synthetic Fingerprint Generation,s A–F) and the functional B-cell subsets (B-1a, B-1b, B-2, and marginal zone [MZ] B cells) in the periphery..Although we focus on murine B-cell subsets, the methods we discuss are relevant to FACS studies conducted with all types of cells and other FACS instruments. We introduce a new method for sca作者: magnanimity 時(shí)間: 2025-3-31 07:03
Brian J. Meacham,Margaret McNameetransfer into recipient mice. The localization of transferred cells in lymphoid organs can be determined by immunohistochemistry and/or flow cytometric analysis of harvested tissues or cells. Because the proper localization of B cells in lymphoid organs is a multistep process, abnormal positioning c作者: Critical 時(shí)間: 2025-3-31 09:32
M. L. Parsons,B. W. Smith,G. E. Bentleyfrom single cells within the population to estimate the level of somatic hypermutation as the best molecular indicator of B-cell memory (.). In this chapter, we describe the methods used in each of these four sections that serve to provide high-resolution quantification of antigen-specific B-cell me作者: 不在灌木叢中 時(shí)間: 2025-3-31 14:03 作者: 戰(zhàn)勝 時(shí)間: 2025-3-31 18:20 作者: excrete 時(shí)間: 2025-3-31 23:25
In Vitro and In Vivo Assays of B-Lymphocyte Migration,transfer into recipient mice. The localization of transferred cells in lymphoid organs can be determined by immunohistochemistry and/or flow cytometric analysis of harvested tissues or cells. Because the proper localization of B cells in lymphoid organs is a multistep process, abnormal positioning c作者: 儀式 時(shí)間: 2025-4-1 03:30
Analysis of Antigen-Specific B-Cell Memory Directly Ex Vivo,from single cells within the population to estimate the level of somatic hypermutation as the best molecular indicator of B-cell memory (.). In this chapter, we describe the methods used in each of these four sections that serve to provide high-resolution quantification of antigen-specific B-cell me作者: outset 時(shí)間: 2025-4-1 08:08
B-Cell Signal Transduction,size the use of phospho-specific antibodies (Abs) and low-molecular-weight enzyme inhibitors in the process of mapping BCR-activated signaling pathways as well as determining activation states of signaling proteins.作者: Veneer 時(shí)間: 2025-4-1 13:21
