標(biāo)題: Titlebook: Axon Regeneration; Methods and Protocol Ava J. Udvadia,James B. Antczak Book 2023 The Editor(s) (if applicable) and The Author(s), under ex [打印本頁] 作者: antibody 時間: 2025-3-21 17:26
書目名稱Axon Regeneration影響因子(影響力)
書目名稱Axon Regeneration影響因子(影響力)學(xué)科排名
書目名稱Axon Regeneration網(wǎng)絡(luò)公開度
書目名稱Axon Regeneration網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Axon Regeneration被引頻次
書目名稱Axon Regeneration被引頻次學(xué)科排名
書目名稱Axon Regeneration年度引用
書目名稱Axon Regeneration年度引用學(xué)科排名
書目名稱Axon Regeneration讀者反饋
書目名稱Axon Regeneration讀者反饋學(xué)科排名
作者: 山間窄路 時間: 2025-3-21 21:35
Defining Selective Neuronal Resilience and Identifying Targets of Neuroprotection and Axon Regeneraur goal is to identify differences in the dynamics of survival among 46 molecularly defined RGC types together with molecular signatures that correlate with these differences. The data consists of scRNA-seq profiles of RGCs collected at six time points following optic nerve crush (ONC) (see companio作者: hauteur 時間: 2025-3-22 03:26
Retinal Ganglion Cell Axon Fractionation,. Here we present a fractionation method to isolate regenerating RGC axons for downstream analysis using immunomagnetic separation of cholera toxin subunit B (CTB)-bound RGC axons. After optic nerve tissue dissection and dissociation, conjugated CTB is used to bind preferentially to regenerated RGC 作者: Inferior 時間: 2025-3-22 05:03
Analysis of Immediate Early Gene Expression Levels to Interrogate Changes in Cortical Neuronal Actie research. Due to straightforward detection methods such as in situ hybridization and immunohistochemistry, changes in IEG expression can be easily visualized across brain regions and in response to physiological and pathological stimulation. Based on in-house experience and existing literature, . 作者: Breach 時間: 2025-3-22 09:03 作者: depreciate 時間: 2025-3-22 15:25
Transneuronal Delivery of Cytokines to Stimulate Mammalian Spinal Cord Regeneration,in regenerating after injury. Many of these key fiber tracts originate from deep brain stem nuclei that are difficult to access. Here we detail a new methodology that achieves functional regeneration in mice after a complete spinal cord crush, describing the crushing procedure itself, intracortical 作者: craven 時間: 2025-3-22 18:32
Epigenomic Profiling of Dorsal Root Ganglia upon Regenerative and Non-regenerative Axonal Injury,e genome-wide techniques that provide information relative to gene expression, chromatin binding sites, and chromatin accessibility, respectively. Here we describe RNA-seq, H3K9ac, H3K27ac and H3K27me3 ChIP-seq, and ATAC-seq in dorsal root ganglia (DRG) after sciatic nerve or dorsal column axotomy, 作者: 山崩 時間: 2025-3-22 22:09 作者: 職業(yè) 時間: 2025-3-23 04:34
Analysis of Axonal Regrowth and Dendritic Remodeling After Optic Nerve Crush in Adult Zebrafish, contrast to mammals, adult zebrafish show a robust regeneration capacity after CNS injury and form the ideal model organism to further unravel the underlying mechanisms for both axonal and dendritic regrowth upon CNS damage. Here, we first describe an optic nerve crush injury model in adult zebrafi作者: 向外 時間: 2025-3-23 08:11 作者: Innovative 時間: 2025-3-23 13:41
Surgical Methods in Postmetamorphic ,: Optic Nerve Crush Injury Model,ferent cell types, retinal ganglion cells (RGCs) are the only cell type connecting the eye to the brain. Optic nerve crush injuries, wherein RGC axons are damaged without severing the optic nerve sheath, can serve as a model for traumatic optical neuropathies as well as some progressive neuropathies作者: 木質(zhì) 時間: 2025-3-23 16:10 作者: essential-fats 時間: 2025-3-23 19:31
A Reproducible Spinal Cord Crush Injury in the Regeneration-Permissive Axolotl,d to severe spinal cord injury by forming a glial scar, which prevents further damage but also inhibits any regenerative growth, resulting in loss of function caudal to the injury site. The axolotl has become a popular system to elucidate the underlying cellular and molecular events that contribute 作者: 大溝 時間: 2025-3-23 23:22 作者: 確定方向 時間: 2025-3-24 05:23 作者: Self-Help-Group 時間: 2025-3-24 08:52 作者: 愛了嗎 時間: 2025-3-24 14:07
Profiling Dynamic Changes in DNA Accessibility During Axon Regeneration After Optic Nerve Crush in accessibility of DNA regulatory elements such as promoters and enhancers over the course of regeneration. This chapter describes methods for preparing ATAC-seq libraries from isolated zebrafish retinal ganglion cells (RGCs) following optic nerve crush at selected post-injury time points. These metho作者: transient-pain 時間: 2025-3-24 16:46 作者: 我吃花盤旋 時間: 2025-3-24 22:31 作者: 針葉樹 時間: 2025-3-24 23:25
978-1-0716-3014-3The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines作者: Painstaking 時間: 2025-3-25 04:36
Hypothekenrecht und Notverordnungen others are more resilient. Identifying molecular features that separate resilient from susceptible populations could reveal potential targets for neuroprotection and axon regeneration. A powerful approach to resolve molecular differences across cell types is single-cell RNA-sequencing (scRNA-seq). 作者: Nonconformist 時間: 2025-3-25 11:05 作者: custody 時間: 2025-3-25 13:55 作者: 擔(dān)憂 時間: 2025-3-25 17:39
John T. Povlishock,Yuji Ueda,Enoch P. Weie research. Due to straightforward detection methods such as in situ hybridization and immunohistochemistry, changes in IEG expression can be easily visualized across brain regions and in response to physiological and pathological stimulation. Based on in-house experience and existing literature, . 作者: 令人不快 時間: 2025-3-25 21:45 作者: 招待 時間: 2025-3-26 03:21
Egon M. R. Doppenberg,Ross Bullockin regenerating after injury. Many of these key fiber tracts originate from deep brain stem nuclei that are difficult to access. Here we detail a new methodology that achieves functional regeneration in mice after a complete spinal cord crush, describing the crushing procedure itself, intracortical 作者: Myocarditis 時間: 2025-3-26 07:11 作者: 討好美人 時間: 2025-3-26 09:54 作者: 認(rèn)識 時間: 2025-3-26 13:07
https://doi.org/10.1007/978-3-7091-5474-8 contrast to mammals, adult zebrafish show a robust regeneration capacity after CNS injury and form the ideal model organism to further unravel the underlying mechanisms for both axonal and dendritic regrowth upon CNS damage. Here, we first describe an optic nerve crush injury model in adult zebrafi作者: insecticide 時間: 2025-3-26 17:41
Florian G. Hartmann,Daniel Loisfore are widely used to dynamically visualize cellular processes in vivo, such as nerve regeneration. Regeneration of retinal ganglion cell (RGC) axons within the optic nerve has been previously studied in adult zebrafish. In contrast, assays of optic nerve regeneration have previously not been esta作者: Detoxification 時間: 2025-3-26 21:08 作者: CON 時間: 2025-3-27 01:29
,Koyré, Cassirer and the History of Science,lesions, as well as after lesions that selectively target specific neuronal cell populations. An advantage of chemical retinal lesion for studying the process of regeneration is that the lesion is topographically widespread. This results in the loss of visual function as well as a regenerative respo作者: 發(fā)怨言 時間: 2025-3-27 08:13
Hypothesis Generation by Difference,d to severe spinal cord injury by forming a glial scar, which prevents further damage but also inhibits any regenerative growth, resulting in loss of function caudal to the injury site. The axolotl has become a popular system to elucidate the underlying cellular and molecular events that contribute 作者: 刺耳的聲音 時間: 2025-3-27 09:30
Hypothesis Generation and Interpretationdeath. Experimentally injuring an axon makes it possible to study degeneration of the distal stump that was detached from the cell body and document the successive steps of regeneration. Precise injury reduces damage to the environment surrounding an axon, and thereby the involvement of extrinsic pr作者: 金盤是高原 時間: 2025-3-27 16:12 作者: 逃避系列單詞 時間: 2025-3-27 21:10 作者: 熱心 時間: 2025-3-27 22:16
https://doi.org/10.1007/978-3-658-27588-4accessibility of DNA regulatory elements such as promoters and enhancers over the course of regeneration. This chapter describes methods for preparing ATAC-seq libraries from isolated zebrafish retinal ganglion cells (RGCs) following optic nerve crush at selected post-injury time points. These metho作者: FLAIL 時間: 2025-3-28 02:10 作者: 懶洋洋 時間: 2025-3-28 08:04
Ava J. Udvadia,James B. AntczakIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: Friction 時間: 2025-3-28 14:08
Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/167744.jpg作者: follicular-unit 時間: 2025-3-28 16:54 作者: forthy 時間: 2025-3-28 19:36 作者: observatory 時間: 2025-3-28 23:09 作者: Fecundity 時間: 2025-3-29 04:53 作者: CAPE 時間: 2025-3-29 08:25 作者: 人充滿活力 時間: 2025-3-29 11:58
1064-3745 ation advice from the experts.This volume covers a wide range of approaches utilized to decipher cellular and molecular mechanisms that contribute to successful nerve regeneration leading to functional recovery. Chapters detail a variety of models utilizing both .in vivo .and .in vitro. approaches, 作者: 樹膠 時間: 2025-3-29 18:51
Hypothermia for Acute Brain Damagee we describe RNA-seq, H3K9ac, H3K27ac and H3K27me3 ChIP-seq, and ATAC-seq in dorsal root ganglia (DRG) after sciatic nerve or dorsal column axotomy, to characterize the transcriptional and epigenetic signatures of DRG upon regenerative vs non-regenerative axonal lesion.作者: Muscularis 時間: 2025-3-29 21:09 作者: 危險 時間: 2025-3-30 03:49 作者: Pcos971 時間: 2025-3-30 08:07 作者: Aggrandize 時間: 2025-3-30 10:58
John T. Povlishock,Yuji Ueda,Enoch P. Weient rise in neuronal activity in visual cortical territory deprived of direct retinal visual input. Here, we describe a protocol for high-throughput radioactive . in situ hybridization as a readout for cortical neuronal activity dynamics in response to partial vision loss in mice.作者: 向下 時間: 2025-3-30 14:36 作者: –吃 時間: 2025-3-30 17:48
https://doi.org/10.1007/978-3-7091-5474-8he brain, using retro- and anterograde tracing experiments and an immunofluorescent staining for presynaptic compartments, respectively. Finally, methods to analyze RGC dendrite retraction and subsequent regrowth in the retina are delineated, using morphological measurements and immunofluorescent staining for dendritic and synaptic markers.作者: Tremor 時間: 2025-3-30 21:13 作者: nautical 時間: 2025-3-31 02:45 作者: 流逝 時間: 2025-3-31 05:32 作者: 清晰 時間: 2025-3-31 12:13 作者: 期滿 時間: 2025-3-31 14:59
Defining Selective Neuronal Resilience and Identifying Targets for Neuroprotection and Axon Regener because it is an experimentally accessible central nervous system tissue and its cell types have been comprehensively characterized by scRNA-seq. This chapter will focus on preparing retinal ganglion cells (RGCs) for scRNA-seq and pre-processing of sequencing results.作者: 猜忌 時間: 2025-3-31 19:15 作者: 或者發(fā)神韻 時間: 2025-4-1 00:35
Profiling Locally Translated mRNAs in Regenerating Axons, sites of protein synthesis using reporter cDNAs that encode two different localizing mRNAs along with diffusion-limited fluorescent reporter proteins. We show how this method can be used to determine how extracellular stimuli and different physiological states can alter the specificity of local mRNA translation in real time.作者: sebaceous-gland 時間: 2025-4-1 04:02
Analysis of Axonal Regrowth and Dendritic Remodeling After Optic Nerve Crush in Adult Zebrafish,he brain, using retro- and anterograde tracing experiments and an immunofluorescent staining for presynaptic compartments, respectively. Finally, methods to analyze RGC dendrite retraction and subsequent regrowth in the retina are delineated, using morphological measurements and immunofluorescent staining for dendritic and synaptic markers.作者: consent 時間: 2025-4-1 10:05 作者: ALIBI 時間: 2025-4-1 11:26
Translating Ribosome Affinity Purification (TRAP) and Bioinformatic RNA-Seq Analysis in Post-metamoof the experimental design and necessary controls and their rationale, along with a description of the bioinformatic steps involved in analyzing the . translatome using TRAP and RNA-Seq, is also provided.
