標(biāo)題: Titlebook: Autophagy and Cancer; Methods and Protocol Helin Norberg,Erik Norberg Book 2022 The Editor(s) (if applicable) and The Author(s), under excl [打印本頁(yè)] 作者: 貧血 時(shí)間: 2025-3-21 18:06
書目名稱Autophagy and Cancer影響因子(影響力)
書目名稱Autophagy and Cancer影響因子(影響力)學(xué)科排名
書目名稱Autophagy and Cancer網(wǎng)絡(luò)公開度
書目名稱Autophagy and Cancer網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Autophagy and Cancer被引頻次
書目名稱Autophagy and Cancer被引頻次學(xué)科排名
書目名稱Autophagy and Cancer年度引用
書目名稱Autophagy and Cancer年度引用學(xué)科排名
書目名稱Autophagy and Cancer讀者反饋
書目名稱Autophagy and Cancer讀者反饋學(xué)科排名
作者: 嚴(yán)峻考驗(yàn) 時(shí)間: 2025-3-21 20:28
Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/166837.jpg作者: amenity 時(shí)間: 2025-3-22 04:14 作者: 不能平靜 時(shí)間: 2025-3-22 08:13
978-1-0716-2073-1The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines作者: 挫敗 時(shí)間: 2025-3-22 12:14 作者: 羞辱 時(shí)間: 2025-3-22 16:35
Gravity, Particles, and Astrophysicslysosomal content, allowing specific lysosomal investigations induced by autophagy. In this protocol chapter, we describe detailed practical instructions and advices for an efficacious lysosomal enrichment and isolation procedure by differential multilayered density gradient centrifugations using hu作者: 異教徒 時(shí)間: 2025-3-22 18:48 作者: 品嘗你的人 時(shí)間: 2025-3-23 00:32
,1905–1930: The Golden Age of Physics,discovered for autophagy. At the same time, precise monitoring of autophagy has become important, and western blotting and fluorescence microscopy of the marker protein LC3 is?widely used for this purpose. Here, we describe a modification of the widely used method, number of LC3 dots per cell. This 作者: 加劇 時(shí)間: 2025-3-23 03:19 作者: 未成熟 時(shí)間: 2025-3-23 09:12 作者: critic 時(shí)間: 2025-3-23 12:52 作者: 通知 時(shí)間: 2025-3-23 16:24
https://doi.org/10.1007/978-981-10-8696-0enetic material during cell division. Detection of mitotic errors such as misaligned chromosomes or chromosomal bridges (also known as lagging chromosomes) is challenging as it requires the analysis and manual discrimination of chromosomal aberrations in mitotic cells by molecular techniques. In int作者: 過(guò)于光澤 時(shí)間: 2025-3-23 20:10 作者: neolith 時(shí)間: 2025-3-23 23:02 作者: 殺死 時(shí)間: 2025-3-24 02:34 作者: PATRI 時(shí)間: 2025-3-24 07:13 作者: 種族被根除 時(shí)間: 2025-3-24 11:08
https://doi.org/10.1007/978-3-319-20224-2ondria during mitophagy is mediated by double-membrane vesicles called autophagosomes, which encapsulate mitochondria that have been tagged for mitophagic removal before delivering them to lysosomes for degradation. A variety of different mitophagy pathways exist that differ in their mechanisms of i作者: 移植 時(shí)間: 2025-3-24 16:58
Stefano Braccini,Francesco Fidecaroal network functionality. The elimination of damaged mitochondria through autophagy requires two steps: induction of general autophagy and priming of damaged mitochondria for selective autophagic recognition. Mitophagy impairment is linked to various pathologies; thus, removal of malfunctioning or e作者: VALID 時(shí)間: 2025-3-24 20:19
Autophagy and Cancer978-1-0716-2071-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 值得 時(shí)間: 2025-3-25 00:15 作者: FLASK 時(shí)間: 2025-3-25 06:51
Measuring Autophagic Cargo Flux with Keima-Based Probes that Torin1 induces autophagic flux of cytosol and mitochondria to a similar degree, that is, compatible with induction of bulk autophagy, whereas DFP induces a highly selective form of mitophagy that efficiently excludes cytosol.作者: 翅膀拍動(dòng) 時(shí)間: 2025-3-25 08:13 作者: 一個(gè)攪動(dòng)不安 時(shí)間: 2025-3-25 13:02
Studying Autophagy Using a TMT-Based Quantitative Proteomics Approachive technique that can examine relative protein abundances in multiple samples (parallel multiplexing). Investigating autophagy through TMT-based MS approach can give great insights into autophagy-regulated biological processes, protein–protein interaction networks, spatiotemporal protein dynamics, 作者: 信任 時(shí)間: 2025-3-25 18:44 作者: Consequence 時(shí)間: 2025-3-25 22:04
1064-3745 d cutting-edge,?.Autophagy and Cancer: Methods and Protocols .aims to be a?useful practical guide to researches to help further their study in this field.?.978-1-0716-2073-1978-1-0716-2071-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: curriculum 時(shí)間: 2025-3-26 03:59
Gravity, Particles, and Astrophysics, we describe the generation of isoform-specific CRISPR-Cas9 genomic editing of LAMP-2A in human cancer cells, without affecting the other two isoforms, allowing for experimental evaluation of LAMP-2A, thus CMA in human cancer models.作者: Sedative 時(shí)間: 2025-3-26 07:51
Physiological Effects of Microgravity, that Torin1 induces autophagic flux of cytosol and mitochondria to a similar degree, that is, compatible with induction of bulk autophagy, whereas DFP induces a highly selective form of mitophagy that efficiently excludes cytosol.作者: BRUNT 時(shí)間: 2025-3-26 12:05
https://doi.org/10.1007/978-94-007-5361-7 ways, mTORC1 activity assay and fluorescent analysis of mTORC1 and lysosome localization as a novel senescence hallmark. Incapability of senescent cells to complete autophagy after damage allows to force them to apoptosis. To demonstrate apoptotic cell death, analysis of caspase activity, Annexin V作者: Vasodilation 時(shí)間: 2025-3-26 16:33 作者: Solace 時(shí)間: 2025-3-26 18:57 作者: Osteons 時(shí)間: 2025-3-26 22:30 作者: 壁畫 時(shí)間: 2025-3-27 03:30 作者: 厭倦嗎你 時(shí)間: 2025-3-27 05:30 作者: 引導(dǎo) 時(shí)間: 2025-3-27 13:19
Concepts and Design of Introducing Synthetic MicroRNAs into Mammalian Cellsd methodological steps to introduce synthetic microRNA drugs into target cells in vitro and in vivo and how to monitor their function. In addition, it provides insights on how to control the adverse effects when ectopically expressing synthetic microRNA mimic molecules.作者: 全神貫注于 時(shí)間: 2025-3-27 14:16 作者: lambaste 時(shí)間: 2025-3-27 21:43 作者: FLOUR 時(shí)間: 2025-3-27 23:23
A Balance Between Autophagy and Other Cell Death Modalities in Cancerophagy-related pathologies gave rise to the development of new therapeutic drugs. Here, we summarize the current knowledge on the autophagic role in disease pathogenesis, particularly in cancer, and the interplay between autophagy and other cell death modalities in order to combat cancer.作者: 抵押貸款 時(shí)間: 2025-3-28 02:56 作者: Cosmopolitan 時(shí)間: 2025-3-28 08:26 作者: cortex 時(shí)間: 2025-3-28 14:24
,1905–1930: The Golden Age of Physics,the marker protein LC3 is?widely used for this purpose. Here, we describe a modification of the widely used method, number of LC3 dots per cell. This protocol provides the proportion of vesicular LC3 staining over the total LC3 staining in the same cell. The approach is well suitable for quantification of endogenous LC3.作者: cloture 時(shí)間: 2025-3-28 16:00
Strings and Fundamental Interactions,cribe a new tool for the qualitative and quantitative determination of autophagic flux using a dual lentiviral reporter system that generates a fusion HiBiT-GFP-LC3B protein suitable for generating stable cell lines.作者: 歡騰 時(shí)間: 2025-3-28 20:04 作者: 定點(diǎn) 時(shí)間: 2025-3-29 00:20
Stefano Braccini,Francesco Fidecarodamaged mitochondria for selective autophagic recognition. Mitophagy impairment is linked to various pathologies; thus, removal of malfunctioning or even harmful mitochondria is vital to cellular physiology. Here, we describe methods that can be applied to the investigation of mitophagy.作者: 量被毀壞 時(shí)間: 2025-3-29 04:45 作者: 者變 時(shí)間: 2025-3-29 10:17
A Dual HiBiT-GFP-LC3 Lentiviral Reporter for Autophagy Flux Assessmentcribe a new tool for the qualitative and quantitative determination of autophagic flux using a dual lentiviral reporter system that generates a fusion HiBiT-GFP-LC3B protein suitable for generating stable cell lines.作者: extinct 時(shí)間: 2025-3-29 14:21 作者: Inordinate 時(shí)間: 2025-3-29 16:33
Induction and Detection of Mitophagydamaged mitochondria for selective autophagic recognition. Mitophagy impairment is linked to various pathologies; thus, removal of malfunctioning or even harmful mitochondria is vital to cellular physiology. Here, we describe methods that can be applied to the investigation of mitophagy.作者: Ornithologist 時(shí)間: 2025-3-29 20:46
Book 2022includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols..?.Authoritative and cutting-edge,?.Autophagy and Cancer: Methods and Protocols .aims to be a?useful practical guide to researches to help further their study in this field.?.作者: 灰姑娘 時(shí)間: 2025-3-30 01:11 作者: 機(jī)制 時(shí)間: 2025-3-30 06:10
Isolation of Autophagy Competent Lysosomes from Cancer Cells by Differential Large-Scale Multilayerel changes by comparative quantitative measurement. This method has been used to enrich lysosomes from a variety of cancer cells with activated chaperone-mediated autophagy, but can be optimized for other cell lines and tissues for multiple autophagy-induced conditions.作者: BLINK 時(shí)間: 2025-3-30 08:59 作者: 協(xié)迫 時(shí)間: 2025-3-30 14:47 作者: judicial 時(shí)間: 2025-3-30 18:23
Isolation of Autophagy Competent Lysosomes from Cancer Cells by Differential Large-Scale Multilayerelysosomal content, allowing specific lysosomal investigations induced by autophagy. In this protocol chapter, we describe detailed practical instructions and advices for an efficacious lysosomal enrichment and isolation procedure by differential multilayered density gradient centrifugations using hu作者: Narcissist 時(shí)間: 2025-3-30 23:26 作者: Debate 時(shí)間: 2025-3-31 01:51
Modified LC3 Dot Quantification Methoddiscovered for autophagy. At the same time, precise monitoring of autophagy has become important, and western blotting and fluorescence microscopy of the marker protein LC3 is?widely used for this purpose. Here, we describe a modification of the widely used method, number of LC3 dots per cell. This 作者: 單調(diào)女 時(shí)間: 2025-3-31 05:54
A Quantitative Flow Cytometry –Based Method for Autophagy Detection Across the Cell Cycle detection and quantification of various cellular markers in live or fixed cells. Here, a flow cytometry–based assay to characterize autophagy across the cell cycle is described. This method is based on selective plasma membrane permeabilization with digitonin and extraction of membrane-unbound LC3 作者: 喃喃而言 時(shí)間: 2025-3-31 11:09 作者: Lipoma 時(shí)間: 2025-3-31 15:17 作者: ANTE 時(shí)間: 2025-3-31 18:48
Detection of Nuclear Biomarkers for Chromosomal Instabilityenetic material during cell division. Detection of mitotic errors such as misaligned chromosomes or chromosomal bridges (also known as lagging chromosomes) is challenging as it requires the analysis and manual discrimination of chromosomal aberrations in mitotic cells by molecular techniques. In int作者: Exhilarate 時(shí)間: 2025-3-31 22:46
Analysis of Autophagic Vesicles in Mitotic Cellsre commonly used in immunofluorescence and biochemistry assays to evaluate the status of autophagy in adherent cells. During mitosis, cells undergo important morphological changes which alter the position of the central plane, therefore the imaging of dividing cells has to be specifically designed.
