標題: Titlebook: Autophagy; Methods and Protocol Nicholas Ktistakis,Oliver Florey Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 20 [打印本頁] 作者: 棕櫚等 時間: 2025-3-21 17:03
書目名稱Autophagy影響因子(影響力)
書目名稱Autophagy影響因子(影響力)學科排名
書目名稱Autophagy網(wǎng)絡公開度
書目名稱Autophagy網(wǎng)絡公開度學科排名
書目名稱Autophagy被引頻次
書目名稱Autophagy被引頻次學科排名
書目名稱Autophagy年度引用
書目名稱Autophagy年度引用學科排名
書目名稱Autophagy讀者反饋
書目名稱Autophagy讀者反饋學科排名
作者: Radiculopathy 時間: 2025-3-21 22:36
Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/166834.jpg作者: Hypomania 時間: 2025-3-22 03:27
Structural Studies of Selective Autophagy in Yeastdelivers some vacuolar enzymes into the vacuole selectively and constitutively, is one of the most characterized examples of selective autophagy in budding yeast. Here we summarize the methods of X-ray crystallography, NMR, and other biophysical analyses to study the structural basis of the Cvt pathway.作者: 人類 時間: 2025-3-22 06:17 作者: 不能仁慈 時間: 2025-3-22 10:30 作者: 討人喜歡 時間: 2025-3-22 13:04
Springer Science+Business Media, LLC, part of Springer Nature 2019作者: bioavailability 時間: 2025-3-22 18:08
Lars E. Sj?berg,Mohammad Bagherbandiscribed is the method to induce p62 filament assembly and their visualization by negative stain electron microscopy (EM). In cells, p62 forms large structures termed p62 bodies and has been shown to be aggregation prone. This tendency to aggregate poses problems for expression and purification in vi作者: 體貼 時間: 2025-3-22 23:44
https://doi.org/10.1007/978-3-642-79880-1strategies to modulate these activities. In this chapter we will describe the work packages required to determine the three-dimensional structures of proteins involved in autophagy by using X-ray crystallography or?nuclear magnetic resonance (NMR) spectroscopy. Further we will provide instructions h作者: ungainly 時間: 2025-3-23 01:31 作者: arboretum 時間: 2025-3-23 06:43 作者: Injunction 時間: 2025-3-23 09:42 作者: Feigned 時間: 2025-3-23 16:01
Christopher D Warner,Michael E Mclntyreegradation. The nucleation of this precursor membrane, termed phagophore, involves the coordinated assembly of the Atg1-kinase complex and the recruitment of Atg9 vesicles. The latter represents one important membrane source in order to produce phagophores in vivo. We explain how the process of phag作者: 抓住他投降 時間: 2025-3-23 19:21
Worldwide Synthetic Gravity Tide Parametersmbrane remodeling process. The early steps involve autophagic membrane precursor generation from the intracellular membranes. The intricate protein-membrane interactions underlying autophagic membrane precursor generation have been a focus of attention but yet poorly defined. Here, we summarize the 作者: 小步走路 時間: 2025-3-23 23:37
An Ocean Bottom Absolute Gravity Meterprocessed C-termini are posttranslationally conjugated to a phosphatidylethanolamine moiety, enabling their insertion into the lipid bilayers of both the inner and outer membranes of the forming autophagosomes. The ATG8s bind a diverse selection of proteins including cargo receptors for selective au作者: Petechiae 時間: 2025-3-24 04:29
British Precise Gravity Network 1993s. Maintaining a functional lysosome pool is critical for generating autolysosomes, in which cellular components are degraded and turned over during autophagy. This unit describes methods to visualize ALR in cells. In addition, this unit provides detailed protocols to establish in vitro systems whic作者: 存在主義 時間: 2025-3-24 10:27 作者: CAMEO 時間: 2025-3-24 13:17 作者: Scintillations 時間: 2025-3-24 17:22
Curvilinear Coordinates and General Tensors,oach can be extended to reveal ultrastructural details in three dimensions. The resolution of electron microscopy is needed when membrane contact sites and tubular connections between organelles are studied.作者: 表狀態(tài) 時間: 2025-3-24 22:48 作者: 搜集 時間: 2025-3-25 01:12 作者: anarchist 時間: 2025-3-25 04:45 作者: 出血 時間: 2025-3-25 09:37
,Einstein’s Law of Gravitation, in neurons are only beginning to be understood. To address this fundamental gap in knowledge, we have established several key methodologies for live-cell imaging and quantitative analysis of autophagy in primary hippocampal neurons. Using these methods, we have defined compartment-specific dynamics作者: Fillet,Filet 時間: 2025-3-25 12:41 作者: Sigmoidoscopy 時間: 2025-3-25 18:58 作者: flaggy 時間: 2025-3-25 19:59 作者: JECT 時間: 2025-3-26 03:18 作者: CANON 時間: 2025-3-26 05:49 作者: Pessary 時間: 2025-3-26 11:35
Formation of Autophagosomes Coincides with Relaxation of Membrane Curvaturerecursors and report that the formation of autophagosomes is associated with a strong relaxation of membrane curvature energy. This effect we find for a wide range of membrane asymmetries. It is especially strong for small distances between both autophagosomal membranes, as observed in vivo. We quan作者: Obstacle 時間: 2025-3-26 13:37 作者: 高度 時間: 2025-3-26 17:10 作者: 偽造者 時間: 2025-3-26 23:12 作者: RAFF 時間: 2025-3-27 02:41
G. Boedecker,I. Marson,H.-G. Wenzelrecursors and report that the formation of autophagosomes is associated with a strong relaxation of membrane curvature energy. This effect we find for a wide range of membrane asymmetries. It is especially strong for small distances between both autophagosomal membranes, as observed in vivo. We quan作者: BLANK 時間: 2025-3-27 06:25
,Experimental Tests of Einstein’s Theory, imaging-based autophagy assessment methodologies can be applied to these neurons, thereby providing a detailed account of the application of imaging-based autophagy assays to human iPSC-derived mDANs.作者: invulnerable 時間: 2025-3-27 11:19
1064-3745 ation advice from the experts.This volume details a comprehensive and extensive set of protocols for the study of autophagy .in vitro. and .in vivo.. Chapters focus on mammals, various model organisms, and provide protocols for the study of autophagy-related processes outside of the canonical autoph作者: agglomerate 時間: 2025-3-27 17:12 作者: GENRE 時間: 2025-3-27 17:53 作者: 是剝皮 時間: 2025-3-27 22:11 作者: 殺人 時間: 2025-3-28 04:58
,Einstein’s Law of Gravitation,gosomes. To do so, we set up three cameras on one microscope to be able to acquire three colors at the same time. Here we describe the setup using a Yokogawa spinning disk confocal microscope (CSU-W1) with Andor TuCam system attaching 3?×?Zyla 4.2 CMOS cameras (Andor) and detail the method for?acquiring live images.作者: Dna262 時間: 2025-3-28 07:59
Structural Studies of Autophagy-Related Proteinsproteins involved in autophagy by using X-ray crystallography or?nuclear magnetic resonance (NMR) spectroscopy. Further we will provide instructions how to perform a molecular dynamics (MD) simulation using GABARAP as example protein.作者: 被告 時間: 2025-3-28 14:28
Cell-Free Reconstitution of Autophagic Membrane Formationmbrane interactions underlying autophagic membrane precursor generation have been a focus of attention but yet poorly defined. Here, we summarize the procedure of a cell-free system we have established to dissect the molecular mechanism of early autophagic membrane generation.作者: 免費 時間: 2025-3-28 17:17 作者: 為現(xiàn)場 時間: 2025-3-28 19:45
Three-Color Simultaneous Live Imaging of Autophagy-Related Structuresgosomes. To do so, we set up three cameras on one microscope to be able to acquire three colors at the same time. Here we describe the setup using a Yokogawa spinning disk confocal microscope (CSU-W1) with Andor TuCam system attaching 3?×?Zyla 4.2 CMOS cameras (Andor) and detail the method for?acquiring live images.作者: 宴會 時間: 2025-3-29 01:52 作者: 保守 時間: 2025-3-29 04:30
Correlative Live-Cell Imaging and Super-Resolution Microscopy of Autophagyes, relocated, and imaged at super resolution by direct stochastic optical reconstruction microscopy. This chapter provides an easy-to-follow protocol along with practical tips and background information to help set up and perform an experiment.作者: negotiable 時間: 2025-3-29 10:47 作者: 群居動物 時間: 2025-3-29 11:56
Book 2019rious model organisms, and provide protocols for the study of autophagy-related processes outside of the canonical autophagy pathways. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary mate作者: 失望未來 時間: 2025-3-29 17:14 作者: 使高興 時間: 2025-3-29 23:26 作者: critic 時間: 2025-3-30 00:45 作者: Increment 時間: 2025-3-30 07:01
Curvilinear Coordinates and General Tensors,es, relocated, and imaged at super resolution by direct stochastic optical reconstruction microscopy. This chapter provides an easy-to-follow protocol along with practical tips and background information to help set up and perform an experiment.作者: RAG 時間: 2025-3-30 11:08 作者: angina-pectoris 時間: 2025-3-30 14:59 作者: 歡樂中國 時間: 2025-3-30 18:42
An Ocean Bottom Absolute Gravity Meter identifying putative LIR motifs in a whole protein sequence using peptide arrays generated by SPOT synthesis on nitrocellulose membranes. The use of two-dimensional peptide arrays allows for further identification of specific residues critical for LIR binding.作者: 時代錯誤 時間: 2025-3-30 21:28
Curvilinear Coordinates and General Tensors,and isolation membrane-associated tubules (IMATs). These methods can be easily applied to cultured mammalian cells for conventional and cutting-edge EM analyses, leading to a better understanding of ultrastructural details in autophagosome formation.作者: 灰姑娘 時間: 2025-3-31 02:41 作者: 泥土謙卑 時間: 2025-3-31 05:06
Recombinant Expression, Purification, and Assembly of p62 Filaments, as well as the preparation of negative stain EM grids to visualize the filaments. In vitro formed p62 filaments can be used to study receptor cargo interactions in minimal reconstituted autophagy model systems.作者: 絆住 時間: 2025-3-31 12:49
Biophysical Studies of LC3 Family Proteinsvesicles, (b) ultracentrifugation and fluorescence methods for assaying protein binding to membranes, and (c) procedures for assessing vesicle–vesicle aggregation and fusion. The latter include methods for intervesicular total lipid mixing, mixing of lipids in the vesicle inner monolayers, and aqueous contents mixing.作者: 易受刺激 時間: 2025-3-31 13:30 作者: 組成 時間: 2025-3-31 18:11
Improved Electron Microscopy Fixation Methods for Tracking Autophagy-Associated Membranes in Cultureand isolation membrane-associated tubules (IMATs). These methods can be easily applied to cultured mammalian cells for conventional and cutting-edge EM analyses, leading to a better understanding of ultrastructural details in autophagosome formation.作者: 潛伏期 時間: 2025-3-31 22:18
Methods for Imaging Autophagosome Dynamics in Primary Neuronsority of autophagosomes in dendrites are stationary, while some exhibit bidirectional movement. These studies establish an initial road map for autophagosome dynamics in each compartment of the neuron and set the stage for a more detailed understanding of neuronal autophagy in stress and disease.作者: 宣誓書 時間: 2025-4-1 04:22 作者: THE 時間: 2025-4-1 06:34 作者: obnoxious 時間: 2025-4-1 12:28
