標(biāo)題: Titlebook: Autophagosome and Phagosome; Vojo Deretic Book 2008 Humana Press 2008 Amino acid.Autolysosomes.Autophagy.Fluorescence Microscopy.Organelle [打印本頁] 作者: graphic 時間: 2025-3-21 17:16
書目名稱Autophagosome and Phagosome影響因子(影響力)
書目名稱Autophagosome and Phagosome影響因子(影響力)學(xué)科排名
書目名稱Autophagosome and Phagosome網(wǎng)絡(luò)公開度
書目名稱Autophagosome and Phagosome網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Autophagosome and Phagosome被引頻次
書目名稱Autophagosome and Phagosome被引頻次學(xué)科排名
書目名稱Autophagosome and Phagosome年度引用
書目名稱Autophagosome and Phagosome年度引用學(xué)科排名
書目名稱Autophagosome and Phagosome讀者反饋
書目名稱Autophagosome and Phagosome讀者反饋學(xué)科排名
作者: 不真 時間: 2025-3-21 21:24
Protein Trafficking into Autophagosomes,utophagosomes during the process of autophagy. Our current knowledge of how autophagosomes form is scarce, and further investigation into the role that other subcellular compartments have in this process is needed.作者: 一罵死割除 時間: 2025-3-22 03:25 作者: 正式演說 時間: 2025-3-22 06:41 作者: 鬧劇 時間: 2025-3-22 11:11
https://doi.org/10.1007/978-3-031-02613-3utophagosomes during the process of autophagy. Our current knowledge of how autophagosomes form is scarce, and further investigation into the role that other subcellular compartments have in this process is needed.作者: prick-test 時間: 2025-3-22 13:59 作者: Basilar-Artery 時間: 2025-3-22 17:47
https://doi.org/10.1007/978-94-009-5259-1harbored, ie immature phagosomes, phagolysosomes, autophagosomes, and ER-derived vacuoles, to cite a few, and decipher the molecular mechanisms involved in survival of pathogens within infected host cells.作者: LIEN 時間: 2025-3-22 22:45
Book 2008s provide researchers with several modern techniques for in vitro and in vivo studies of phagosomal organelles. Comprehensive and forward-thinking, Autophagosome and Phagosome offers a valuable guide to both cellular processes while inciting researchers to explore the potentially important connections between the two..作者: Evolve 時間: 2025-3-23 02:00 作者: Priapism 時間: 2025-3-23 09:37
https://doi.org/10.1007/978-3-031-02612-6 starvation-induced autophagic activity, and detecting LC3 by immunoblotting or immunofluorescence has become a reliable method for monitoring autophagy and autophagy-related processes, including autophagic cell death. Here we describe basic protocols to assay for endogenous LC3-II by immunoblotting, immunoprecipitation, and immunofluorescence.作者: 國家明智 時間: 2025-3-23 11:43
https://doi.org/10.1007/978-3-658-16013-5autophagy and its effects on cell function. We also discuss potential pitfalls. Although cell lines are used as model systems, the substances and methods described here can be applied to primary cells and tissues.作者: Opponent 時間: 2025-3-23 14:26
Gravity Field and Dynamics of the Earthquantified in a presentation assay with antigen-specific CD4. T-cell clones. These assays are good measures of autophagosome formation and lysosomal degradation of macroautophagy cargo and therefore are useful for studying regulation of the autophagic pathway under various experimental conditions and physiological perturbations.作者: Fibroid 時間: 2025-3-23 19:55 作者: REIGN 時間: 2025-3-24 01:34
Wolfgang Jacoby Johannes,Peter L. Smilde loaded endosomes and magnetic streptavidin conjugated bead-containing phagosomes. Fusion is quantified using a fluorescence-based detection method that measures the peroxidase activity associated with the beads.作者: 翅膀拍動 時間: 2025-3-24 03:15 作者: 卜聞 時間: 2025-3-24 07:37
LC3 and Autophagy, starvation-induced autophagic activity, and detecting LC3 by immunoblotting or immunofluorescence has become a reliable method for monitoring autophagy and autophagy-related processes, including autophagic cell death. Here we describe basic protocols to assay for endogenous LC3-II by immunoblotting, immunoprecipitation, and immunofluorescence.作者: disparage 時間: 2025-3-24 13:44 作者: 征稅 時間: 2025-3-24 18:20
Localization and MHC Class II Presentation of Antigens Targeted for Macroautophagy,quantified in a presentation assay with antigen-specific CD4. T-cell clones. These assays are good measures of autophagosome formation and lysosomal degradation of macroautophagy cargo and therefore are useful for studying regulation of the autophagic pathway under various experimental conditions and physiological perturbations.作者: 平淡而無味 時間: 2025-3-24 19:47 作者: 使乳化 時間: 2025-3-24 23:44
,In Vitro Phagosome–Endosome Fusion, loaded endosomes and magnetic streptavidin conjugated bead-containing phagosomes. Fusion is quantified using a fluorescence-based detection method that measures the peroxidase activity associated with the beads.作者: Allege 時間: 2025-3-25 06:35 作者: 聲明 時間: 2025-3-25 10:41
Amino Acid Regulation of Autophagosome Formation,dependent signaling is subject to complex regulation. The mechanism by which amino acids stimulate mTORdependent signaling (and other signaling pathways), and its molecular connection with the autophagic machinery, is still unknown.作者: debunk 時間: 2025-3-25 15:18 作者: Efflorescent 時間: 2025-3-25 17:43 作者: 不合 時間: 2025-3-25 20:38 作者: 希望 時間: 2025-3-26 02:53
Fine Structure of the Autophagosome,opy remains one of the most accurate methods for quantitation of autophagic vacuole accumulation. Therefore, quantitation of autophagic vacuoles by electron microscopy and point counting is also described. Finally, a short description is given for preparation of ultra thin cryosections for immunogold labeling of autophagic vacuoles.作者: thwart 時間: 2025-3-26 07:37 作者: DALLY 時間: 2025-3-26 09:11 作者: 小蟲 時間: 2025-3-26 13:15
https://doi.org/10.1007/978-981-15-5709-5equestering cellular exterior. Both autophagosomes and phagosomes mature into acidified, degradative organelles, termed autolysosomes and phagolysosomes, respectively. The basic role of autophagy as a nutritional process, and that of phagocytosis where applicable, has survived in present-day organis作者: Adrenaline 時間: 2025-3-26 16:59 作者: 虛度 時間: 2025-3-26 21:48
https://doi.org/10.1007/978-3-031-02613-3utilizing fluorescence microscopy to monitor the localization, and therefore the movement, of membrane-associated Atg proteins. We describe here a method that allows visualization of Atg membrane proteins in order to observe their potential source membranes and also to determine the temporal order o作者: gratify 時間: 2025-3-27 04:57 作者: 提名 時間: 2025-3-27 07:44 作者: 含糊 時間: 2025-3-27 11:02
1064-3745 hagosomal organelles. Comprehensive and forward-thinking, Autophagosome and Phagosome offers a valuable guide to both cellular processes while inciting researchers to explore the potentially important connections between the two..978-1-61737-806-5978-1-59745-157-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 易彎曲 時間: 2025-3-27 17:02 作者: gerontocracy 時間: 2025-3-27 19:00 作者: SAGE 時間: 2025-3-28 01:43 作者: 占線 時間: 2025-3-28 05:17
Microautophagy in the Yeast Saccharomyces cerevisiae, microautophagy only could be characterized microscopically. Recent studies provided the possibility to study the process in vitro and have identified the first molecules that are involved in microautophagy作者: 一罵死割除 時間: 2025-3-28 08:32
Analysis of Phosphoinositide Dynamics During Phagocytosis Using Genetically Encoded Fluorescent Biogged probes (biosensors) into cultured macrophages. These biosensors are based on the fusion of phosphoinositide-binding protein domains with fluorescent proteins. Some specifications for live cell imaging of such phosphoinositide-specific probes are also provided.作者: HEW 時間: 2025-3-28 11:20 作者: choleretic 時間: 2025-3-28 16:58
Fine Structure of the Autophagosome,en for the preparation of cells for conventional electron microscopy and for the identification of autophagic vacuoles by morphology. Electron microscopy remains one of the most accurate methods for quantitation of autophagic vacuole accumulation. Therefore, quantitation of autophagic vacuoles by el作者: 免除責(zé)任 時間: 2025-3-28 19:19
Methods for Assessing Autophagy and Autophagic Cell Death,lack signs of apoptosis (type 1 cell death). Here we detail and critically assess a series of methods to promote and inhibit autophagy via pharmacological and genetic manipulations. We also review the techniques currently available to detect autophagy, including transmission electron microscopy, hal作者: 罵人有污點(diǎn) 時間: 2025-3-28 23:52 作者: cringe 時間: 2025-3-29 04:18
Amino Acid Regulation of Autophagosome Formation,. One of these signaling pathways is mTOR-dependent and is activated by amino acids (leucine in particular) in synergy with insulin. Activation of this pathway inhibits autophagy. Because activation of mTOR-mediated signaling also stimulates protein synthesis, it appears that protein synthesis and a作者: 巨大沒有 時間: 2025-3-29 11:11
Autophagic Proteolysis of Long-Lived Proteins in Nonliver Cells,uester cytoplasmic constituents, including macromolecules such as long-lived proteins. Upon fusion of autophagosomes with lysosomes, the engulfed cargo is degraded. The proteolysis of longlived proteins by macroautophagy is a standard, specific measure of autophagic degradation and represents an end作者: 偽造者 時間: 2025-3-29 15:23
Autophagosomes in GFP-LC3 Transgenic Mice,to identify autophagic structures easily and accurately by fluorescent microscopy. The most widely used marker for autophagosome is LC3, a mammalian homolog of Atg8. To analyze autophagy in whole animals, we generated GFP-LC3 transgenic mice and describe here how we determine the occurrence of autop作者: Medicaid 時間: 2025-3-29 17:41
Analysis of Autophagosome Membrane Cycling by Fluorescence Microscopy,y, and certain cancers. Although nearly 30 autophagy-related (.) genes have been identified and characterized, the molecular mechanisms of this process are only partially understood. One aspect of the pathway that has been intensely studied is the identity of the membrane source for newly formed aut作者: BRAND 時間: 2025-3-29 23:44
Protein Trafficking into Autophagosomes,ted, to later secretory compartments, such as the trans-Golgi network (TGN) or endosome. These two subcellular compartments are closely linked through extensive protein trafficking, in both an anterograde and a retrograde direction. These compartments are likely to be important in the formation of a作者: 忍受 時間: 2025-3-30 03:00 作者: FUME 時間: 2025-3-30 07:10
Molecular Links Between Autophagy and Apoptosis,s have been found between apoptosis and autophagy where inducers of apoptosis also induce autophagy and vice versa. In some cases, autophagy delays the onset of apoptosis and thus prolongs life although it may also promote apoptosis and other forms of cell death. It is thus of great biological and m作者: 厚臉皮 時間: 2025-3-30 08:20
Clearance of Mutant Aggregate-Prone Proteins by Autophagy,s or proteinopathies. We have shown that autophagy, a cytosolic, non-specific bulk degradation system, is an important clearance route for many cytosolic toxic, aggregate-prone proteins, like mutant huntingtin and mutant .-synucleins. Induction of autophagy enhances the clearance of both soluble and作者: 半球 時間: 2025-3-30 14:25
Localization and MHC Class II Presentation of Antigens Targeted for Macroautophagy,MHC class II presentation of potential vaccine antigens, we have developed a method to target antigens for autophagic degradation via fusion to the Atg8/LC3 protein: Atg8/LC3 is specifically incorporated into autophagosomes via coupling to phosphatidylethanolamine, and subsequently degraded in MHC c作者: 恩惠 時間: 2025-3-30 17:41 作者: 鳴叫 時間: 2025-3-30 22:44 作者: surmount 時間: 2025-3-31 03:29 作者: 名次后綴 時間: 2025-3-31 08:55 作者: 咯咯笑 時間: 2025-3-31 11:39 作者: 討人喜歡 時間: 2025-3-31 17:18 作者: 編輯才信任 時間: 2025-3-31 19:47
https://doi.org/10.1007/978-981-15-5709-5terior, respectively. In their most primordial form, both processes are involved in cellular metabolism and feeding, supplying cells with externally obtained particulate nutrients or using portions of cell’s own cytoplasm to generate essential nutrients and energy at times of starvation. Although au作者: craving 時間: 2025-4-1 00:10 作者: Spina-Bifida 時間: 2025-4-1 02:04 作者: 發(fā)怨言 時間: 2025-4-1 06:15 作者: 手勢 時間: 2025-4-1 13:06
https://doi.org/10.1007/978-3-031-23770-6. One of these signaling pathways is mTOR-dependent and is activated by amino acids (leucine in particular) in synergy with insulin. Activation of this pathway inhibits autophagy. Because activation of mTOR-mediated signaling also stimulates protein synthesis, it appears that protein synthesis and a作者: paradigm 時間: 2025-4-1 17:57
Field Theory of Linearised Gravity,uester cytoplasmic constituents, including macromolecules such as long-lived proteins. Upon fusion of autophagosomes with lysosomes, the engulfed cargo is degraded. The proteolysis of longlived proteins by macroautophagy is a standard, specific measure of autophagic degradation and represents an end作者: 法律 時間: 2025-4-1 19:36
https://doi.org/10.1007/978-3-031-02613-3to identify autophagic structures easily and accurately by fluorescent microscopy. The most widely used marker for autophagosome is LC3, a mammalian homolog of Atg8. To analyze autophagy in whole animals, we generated GFP-LC3 transgenic mice and describe here how we determine the occurrence of autop作者: 他很靈活 時間: 2025-4-2 01:16
https://doi.org/10.1007/978-3-031-02613-3y, and certain cancers. Although nearly 30 autophagy-related (.) genes have been identified and characterized, the molecular mechanisms of this process are only partially understood. One aspect of the pathway that has been intensely studied is the identity of the membrane source for newly formed aut作者: optional 時間: 2025-4-2 03:52 作者: Indurate 時間: 2025-4-2 07:35
,Indirekter Nachweis: Bin?rpulsare,ostat system, in which ceramide promotes cell death and growth arrest, and S1P induces proliferation and maintains cell survival. As macroautophagy is a lysosomal catabolic mechanism involved in determining the duration of the lifetime of cells, we raised the question of its regulation by sphingolip
