標題: Titlebook: Auditory and Vestibular Research; Methods and Protocol Bernd Sokolowski Book 2009 Humana Press 2009 cells.gene expression.gene transfer.gen [打印本頁] 作者: 不要提吃飯 時間: 2025-3-21 19:18
書目名稱Auditory and Vestibular Research影響因子(影響力)
書目名稱Auditory and Vestibular Research影響因子(影響力)學(xué)科排名
書目名稱Auditory and Vestibular Research網(wǎng)絡(luò)公開度
書目名稱Auditory and Vestibular Research網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Auditory and Vestibular Research被引頻次
書目名稱Auditory and Vestibular Research被引頻次學(xué)科排名
書目名稱Auditory and Vestibular Research年度引用
書目名稱Auditory and Vestibular Research年度引用學(xué)科排名
書目名稱Auditory and Vestibular Research讀者反饋
書目名稱Auditory and Vestibular Research讀者反饋學(xué)科排名
作者: 寬宏大量 時間: 2025-3-21 23:10 作者: 減震 時間: 2025-3-22 00:31
The Practical Use of Cre and loxP Technologies in Mouse Auditory Researchoxed mice to inactivate genes of their interest specifically in inner ear hair cells. We provide detailed protocols for the use of these techniques and reagents. These considerations and protocols can be easily applied to other cell types in the inner ear and other parts of the auditory pathways. Be作者: Lamina 時間: 2025-3-22 06:54 作者: Humble 時間: 2025-3-22 09:02
Molecular Biology of Vestibular Schwannomasy to successfully treat vestibular schwannomas. Additionally, the signaling pathways affected by merlin, the product of the . gene, are becoming better understood. .-transgenic and knockout mice as well as vestibular schwannoma xenograft models are now ready for novel therapeutic testing. Hopefully,作者: 不朽中國 時間: 2025-3-22 15:41 作者: 翻動 時間: 2025-3-22 17:23 作者: pulmonary-edema 時間: 2025-3-22 23:04 作者: 泛濫 時間: 2025-3-23 03:02 作者: LEVY 時間: 2025-3-23 05:51 作者: plasma-cells 時間: 2025-3-23 10:28 作者: 蝕刻術(shù) 時間: 2025-3-23 17:05 作者: 紋章 時間: 2025-3-23 21:29 作者: asthma 時間: 2025-3-23 22:18 作者: 動作謎 時間: 2025-3-24 05:36 作者: ACE-inhibitor 時間: 2025-3-24 07:07
https://doi.org/10.1007/978-1-4614-5532-5ic mutational analysis of identified residues, leading to the identification of functionally important residues and/or domains in membrane proteins, in the absence of structural information apart from the primary amino acid sequence. This approach is potentially useful in the context of the auditory作者: APEX 時間: 2025-3-24 11:01
Book 2009 be regained partly through the use of a bionic device, known as a cochlear implant. Presently, this device and the auditory brainstem implant are the only ones to prosthetically replace brain function. With the advent of molecular biology tools, such as RT-PCR, the auditory and vestibular ?elds hav作者: 人類學(xué)家 時間: 2025-3-24 15:32 作者: 高歌 時間: 2025-3-24 21:50
Gaming Media and Social Effects require the refinement and adaptation of in situ hybridization methods. The present chapter provides a detailed in situ hybridization protocol adapted to frozen tissue sections collected from adult and neonatal stages of the vertebrate cochlea.作者: 擋泥板 時間: 2025-3-25 01:58 作者: Catheter 時間: 2025-3-25 05:21 作者: 尋找 時間: 2025-3-25 09:02 作者: Eclampsia 時間: 2025-3-25 14:15
In the Beginning was Game Semantics?his chapter provides a detailed description and critical evaluation of the methodology used to transfect cDNA expression constructs, including green fluorescent protein (GFP) tagged full-length cDNAs of myosin XVa, whirlin, and β-actin, into cultured inner ear sensory epithelia using the Bio-Rad Helios? Gene Gun.作者: 男生如果明白 時間: 2025-3-25 16:48 作者: 獨裁政府 時間: 2025-3-25 22:44
Helios? Gene Gun–Mediated Transfection of the Inner Ear Sensory Epitheliumhis chapter provides a detailed description and critical evaluation of the methodology used to transfect cDNA expression constructs, including green fluorescent protein (GFP) tagged full-length cDNAs of myosin XVa, whirlin, and β-actin, into cultured inner ear sensory epithelia using the Bio-Rad Helios? Gene Gun.作者: 單調(diào)女 時間: 2025-3-26 01:23
Electroporation-Mediated Gene Transfer to the Developing Mouse Inner Earsented. Expression plasmid encoding a gene of interest is microinjected through the uterine wall into the lumen of the otocyst and electroporated into otic epithelial progenitor cells. Downstream analysis of the transfected embryonic or postnatal inner ear is then conducted to gain insight into gene function.作者: 不可思議 時間: 2025-3-26 07:19
1064-3745 croscopic imaging.Clearly describes the protocols necessary Hearing is a sensory modality critical to both language and cognitive development. In its absence, and without sensory input through another modality, such as the manual/visual modality of sign language, cognitive and language development c作者: N斯巴達人 時間: 2025-3-26 08:36
Wildlife Overpopulation Controlcharacterization of isolates’ genetic relatedness, yet they differ in the types of studies for which they are most useful. Consequently, knowledge of both techniques is important for laboratories intending to study the molecular epidemiology of otitis media–associated bacterial pathogens.作者: Musculoskeletal 時間: 2025-3-26 15:33
Gamete/Embryo-Fetal Origins of Tumours,shears off bundles and leaves them in agarose blocks. With care in the dissection and in clean-up of the isolated bundles, contamination from cell bodies can be kept to a minimum. Isolated bundles can be analyzed by a variety of techniques, including immunocytochemistry, SDS-PAGE, immunoblotting, and mass spectrometry.作者: 小說 時間: 2025-3-26 19:47 作者: 無禮回復(fù) 時間: 2025-3-26 21:27 作者: outset 時間: 2025-3-27 04:16
Book 2009 such as the manual/visual modality of sign language, cognitive and language development can be severely impaired in the earliest formative years of a child. In its endeavor to d- cover the mechanisms underlying audition, the ?eld of auditory science has provided rich comparative physiological studi作者: Prologue 時間: 2025-3-27 05:43
Janet E. Alton,J. D. A. Widdowsonitable for microarray analysis of inner ear organs, and for cloning of large transcripts, such as those for ion channels. Genetic sequences cloned with these procedures can be used for transient transfection of . kidney cell lines with GFP fusion constructs.作者: 小丑 時間: 2025-3-27 09:57
https://doi.org/10.1007/978-981-287-518-1ereby a library of DNA tags was developed to allow clonal relationships to be confirmed by PCR amplification and sequencing of the tag in dispersed clonal progeny. By varying the site and time of viral delivery, these methods are appropriate to study cell lineages in other tissues of the developing chicken.作者: acetylcholine 時間: 2025-3-27 15:00 作者: paleolithic 時間: 2025-3-27 21:37 作者: 破譯密碼 時間: 2025-3-28 00:53 作者: 駭人 時間: 2025-3-28 02:42
The Use of 2-D Gels to Identify Novel Protein–Protein Interactions in the Cochlea without . knowledge of which one(s) might be relevant. Here, we report the methods by which membrane proteins are isolated from cochlear tissues and prepared for identification by mass spectrometry techniques.作者: 難解 時間: 2025-3-28 07:18
RNA Isolation from Xenopus Inner Ear Sensory Endorgans for Transcriptional Profiling and Molecular Cor experimental manipulation during animal development. However, many procedures for analyzing gene expression in the peripheral auditory and vestibular systems mandate the ability to isolate intact RNA from inner ear tissue. Methods presented here facilitate preparation of high quality inner ear RN作者: Vasoconstrictor 時間: 2025-3-28 11:40
Synthesis of Biotin-Labeled RNA for Gene Expression Measurements Using Oligonucleotide Arraysmeasurements of gene expression as part of carefully designed experiments will continue to further our understanding of audition and have the potential to open up new avenues of research. This chapter describes a reliable protocol to prepare high-quality biotin-labeled RNA target, specifically for o作者: Ascribe 時間: 2025-3-28 18:23 作者: 豐滿中國 時間: 2025-3-28 21:22
Lineage Analysis of Inner Ear Cells Using Genomic Tags for Clonal Identificationmigrational boundaries of individual clones within the inner ear. This chapter details the basic methods for performing lineage analysis of the inner ear using replication-defective retroviral vectors in chicken embryos. Protocols are provided for generating avian retroviruses pseudotyped with vesic作者: GRAVE 時間: 2025-3-29 01:39 作者: 辭職 時間: 2025-3-29 06:52 作者: 懲罰 時間: 2025-3-29 07:43 作者: ALERT 時間: 2025-3-29 12:38
Electroporation-Mediated Gene Transfer to the Developing Mouse Inner Eardges cinch together to establish a fluid-filled sac called the otic vesicle or otocyst. The progenitor cells lining the otocyst lumen will give rise to sensory and non-sensory cells of the inner ear. These formative stages of inner ear development are initiated during the first week of postimplantat作者: Vasodilation 時間: 2025-3-29 18:00
Isolation of Sphere-Forming Stem Cells from the Mouse Inner Earhearing loss as a result of either ototoxic insult or the aging process. Coincidently, with this inability to regenerate lost hair cells, the adult cochlea does not appear to harbor cells with a proliferative capacity that could serve as progenitor cells for lost cells. In contrast, adult mammalian 作者: 冷峻 時間: 2025-3-29 22:53
Molecular Biology of Vestibular Schwannomasr schwannoma research is the development of new treatment options; however, such options have not yet been established. A fundamental understanding of the underlying molecular events leading to tumor formation began when mutations in the . tumor suppressor gene were identified in vestibular schwanno作者: 保全 時間: 2025-3-30 01:11 作者: 假 時間: 2025-3-30 06:28 作者: 使激動 時間: 2025-3-30 10:18 作者: 織物 時間: 2025-3-30 12:46 作者: 國家明智 時間: 2025-3-30 18:36 作者: 6Applepolish 時間: 2025-3-30 21:37
The Use of 2-D Gels to Identify Novel Protein–Protein Interactions in the Cochleaies of protein-protein interactions have gained from the development of techniques and technologies such as immunoprecipitation, preparative two-dimensional (2-D) gel electrophoresis for peptide mass fingerprinting (PMF), using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) m作者: 走調(diào) 時間: 2025-3-31 02:38 作者: BRIEF 時間: 2025-3-31 08:18 作者: progestogen 時間: 2025-3-31 10:28
Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/165032.jpg作者: 假 時間: 2025-3-31 15:43 作者: indicate 時間: 2025-3-31 21:24
Janet E. Alton,J. D. A. Widdowsonmeasurements of gene expression as part of carefully designed experiments will continue to further our understanding of audition and have the potential to open up new avenues of research. This chapter describes a reliable protocol to prepare high-quality biotin-labeled RNA target, specifically for o
