標(biāo)題: Titlebook: Atomic Force Microscopy; Methods and Protocol Nuno C. Santos,Filomena A. Carvalho Book 2019 Springer Science+Business Media, LLC, part of S [打印本頁] 作者: Inveigle 時(shí)間: 2025-3-21 16:27
書目名稱Atomic Force Microscopy影響因子(影響力)
書目名稱Atomic Force Microscopy影響因子(影響力)學(xué)科排名
書目名稱Atomic Force Microscopy網(wǎng)絡(luò)公開度
書目名稱Atomic Force Microscopy網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Atomic Force Microscopy被引頻次
書目名稱Atomic Force Microscopy被引頻次學(xué)科排名
書目名稱Atomic Force Microscopy年度引用
書目名稱Atomic Force Microscopy年度引用學(xué)科排名
書目名稱Atomic Force Microscopy讀者反饋
書目名稱Atomic Force Microscopy讀者反饋學(xué)科排名
作者: Lipohypertrophy 時(shí)間: 2025-3-21 20:44 作者: appall 時(shí)間: 2025-3-22 01:09 作者: transient-pain 時(shí)間: 2025-3-22 07:15
Investigating the Nanodomain Organization of Rhodopsin in Native Membranes by Atomic Force Microscop.e., nanodomains), which facilitates their function in cell signaling processes. The visualization of membrane proteins and nanodomains within biological membranes under physiological conditions presents a challenge and is not possible using conventional microscopy methods. Atomic force microscopy (作者: 祖?zhèn)髫?cái)產(chǎn) 時(shí)間: 2025-3-22 12:20
Analysis of DNA–Protein Complexes by Atomic Force Microscopy Imaging: The Case of TRF2–Telomeric DNAe. For 30?years, AFM has been a valuable tool in life sciences to study biological samples in the field of tissue, cellular and molecular imaging, of mechanical properties and of force spectroscopy. Since the early beginnings of the technique, AFM has been extensively exploited as an imaging tool fo作者: Atrium 時(shí)間: 2025-3-22 14:36 作者: Lyme-disease 時(shí)間: 2025-3-22 17:21
Functionalization of AFM Tips and Supports for Molecular Recognition Force Spectroscopy and Recognitected and localized on the sample surface. Moreover, the mechanism of interaction can be studied by force spectroscopy if purified target molecules are linked to an ultra-flat surface, such as mica or silicon (nitride). Rapid imaging of the binding sites and force spectroscopy studies are greatly fa作者: 浪費(fèi)物質(zhì) 時(shí)間: 2025-3-23 00:29 作者: 哪有黃油 時(shí)間: 2025-3-23 02:17
Single-Molecule Force Spectroscopy: Experiments, Analysis, and Simulations. The quantification of mechanical resistance of cells and biomolecules using biophysical methods matured thanks to the development of nanotechnologies such as optical and magnetic tweezers, the biomembrane force probe, and atomic force microscopy (AFM). The quantitative nature of force spectroscopy作者: Mortal 時(shí)間: 2025-3-23 05:37
AFM to Study Pore-Forming Proteinse surface structure and in the force mode it can also be used to test the mechanical properties of the sample. AFM has been successfully applied to study the molecular mechanism of pore-forming proteins on model membranes. It gives information about both the structural reorganization of the membrane作者: Dorsal-Kyphosis 時(shí)間: 2025-3-23 11:47
Imaging and Manipulation of Extracellular Traps by Atomic Force Microscopygen invasion and proliferation. Notwithstanding their protective role, NETs have also been linked to the development of a variety of disorders, including cardiovascular and autoimmune diseases. Since the first reports on NETs in 2004 it has been possible to image NETs by a variety of imaging techniq作者: 返老還童 時(shí)間: 2025-3-23 17:21
Investigation of Bacterial Curli Production and Adhesion Using AFMs for following bacterial attachment to glass surfaces and provide protocols for coupling bacterial cells to AFM tips. Using single microbial cell force spectroscopy in physiological environment, we show methods to probe mechanical parameters and the dissociation of curliated . cells from fibronecti作者: Communal 時(shí)間: 2025-3-23 20:05
Antimicrobial Peptides: Effect on Bacterial Cellsrce spectroscopy tool, has been applied to study their mechanism of action and development. Here, we describe different methods to be applied in the study of AMP effects on bacteria, either by imaging or by force spectroscopy studies, essential to underlie their action and to identify possibly outco作者: 擔(dān)心 時(shí)間: 2025-3-23 23:59
AFM Nanoindentation Experiments on Protein Shells: A Protocolf other protein shells exist. To deepen our knowledge on the structure and function of protein shells, a profound insight into their mechanical properties is required. Nanoindentation measurements with an atomic force microscope (AFM) are increasingly being performed to probe such material propertie作者: Laconic 時(shí)間: 2025-3-24 04:32 作者: Commodious 時(shí)間: 2025-3-24 10:21
Book 2019e preparation, data acquisition and processing, and avoiding technical problems. Divided into two sections, chapters guide readers through image artifacts, process and quantitatively analyze AFM images, lipid bilayers, image DNA-protein complexes, AFM cell topography, single-molecule force spectrosc作者: Oversee 時(shí)間: 2025-3-24 11:10
1064-3745 experts.This book aims to provide examples of applications of atomic force microscopy (AFM) using biological samples, showing different methods for AFM sample preparation, data acquisition and processing, and avoiding technical problems. Divided into two sections, chapters guide readers through imag作者: Generator 時(shí)間: 2025-3-24 16:01
Francesca A. Lisi,Umberto Stracciace spectroscopy in physiological environment, we show methods to probe mechanical parameters and the dissociation of curliated . cells from fibronectin surfaces by quantifying Young’s modulus, unbinding forces, and de-adhesion works.作者: instill 時(shí)間: 2025-3-24 22:44 作者: 赦免 時(shí)間: 2025-3-24 23:55 作者: 催眠藥 時(shí)間: 2025-3-25 05:45 作者: floaters 時(shí)間: 2025-3-25 07:58 作者: 競選運(yùn)動(dòng) 時(shí)間: 2025-3-25 12:53 作者: heart-murmur 時(shí)間: 2025-3-25 16:40
https://doi.org/10.1007/978-3-319-26656-5 surface and the changes in the force required for membrane piercing upon incubation with this special type of proteins. Here we describe robust protocols to investigate the effect of pore-forming proteins in supported lipid bilayers.作者: Limpid 時(shí)間: 2025-3-25 20:26
Artifacts and Practical Issues in Atomic Force Microscopyn many cases, training in AFM is inadequate. In this chapter we show examples of common artifacts in AFM and describe, where possible, how to overcome them. Other practical issues important for best practice in AFM operation, such as noise reduction and data processing, are also discussed.作者: 中子 時(shí)間: 2025-3-26 03:06
Imaging Artificial Membranes Using High-Speed Atomic Force MicroscopyIn this context, we have developed new protocols adapted for HS-AFM to form supported lipid bilayers on small mica disks using the vesicle fusion or Langmuir-Blodgett methods. In this chapter we describe in detail the protocols to fabricate supported artificial bilayers as well as the main guidelines for HS-AFM imaging of such samples.作者: ALE 時(shí)間: 2025-3-26 06:59 作者: 貪婪地吃 時(shí)間: 2025-3-26 11:05
Ligand-Receptor Binding on Cell Membrane: Dynamic Force Spectroscopy Applicationss, disease pathogenesis, and mechanism of drugs. Here we describe an example of applying single-molecule dynamic force spectroscopy to study the binding of epidermal growth factor (EGF) to its receptor EGFR, as well as the effect of two clinical drugs, Pertuzumab and Trastuzumab, on the interaction of EGF and EGFR.作者: electrolyte 時(shí)間: 2025-3-26 15:56 作者: Respond 時(shí)間: 2025-3-26 20:04
https://doi.org/10.1007/978-3-7908-1835-2roteins. Herein, a method for analyzing a complex formed by a telomeric DNA sequence wrapped around the TRF2 binding protein is presented. The described procedure could be applied to the study of any type of DNA–protein complex.作者: 使入迷 時(shí)間: 2025-3-27 00:19 作者: 頌揚(yáng)國家 時(shí)間: 2025-3-27 02:27 作者: innovation 時(shí)間: 2025-3-27 08:13
AFM Nanoindentation Experiments on Protein Shells: A Protocoln, AFM imaging and nanoindentation, and the subsequent data analysis of the force–distance curves. Whereas AFM can be operated in air and in liquid, the described methods are for probing single particles in liquid to enable studies in close to physiological environments.作者: Veneer 時(shí)間: 2025-3-27 10:48
Francisco Herrera,José Luis Verdegayere we simply quantify the % of domain area, number of domains, and morphology, and quantify how many images must be taken to obtain reliable statistics. We then look at dynamics, describing the methods we use to study the nanometer scale motion of the domain perimeter as observed using Fast Scan AFM, and hence extract a quantitative line tension.作者: 輕推 時(shí)間: 2025-3-27 16:48
Studies in Fuzziness and Soft Computinget of methods by which a variety of proteins, oligonucleotides, or small molecules can be tethered to silicon (nitride) tips or to mica. Methods are included which afford site-specific and oriented coupling of the sensor molecules.作者: constitute 時(shí)間: 2025-3-27 20:03 作者: 半圓鑿 時(shí)間: 2025-3-27 23:26
Functionalization of AFM Tips and Supports for Molecular Recognition Force Spectroscopy and Recognitet of methods by which a variety of proteins, oligonucleotides, or small molecules can be tethered to silicon (nitride) tips or to mica. Methods are included which afford site-specific and oriented coupling of the sensor molecules.作者: Barrister 時(shí)間: 2025-3-28 04:28 作者: medium 時(shí)間: 2025-3-28 09:50
https://doi.org/10.1007/978-3-7908-1835-2y visualize the packing of rhodopsin in native retinal membranes and the quantitative analysis of AFM images is beginning to reveal insights about the nanodomain organization of rhodopsin in the membrane. In this report, we outline procedures for imaging rhodopsin nanodomains by AFM and the quantita作者: Astigmatism 時(shí)間: 2025-3-28 11:02 作者: PALSY 時(shí)間: 2025-3-28 17:53
Jér?me Fortin,Adam Kasperski,Pawe? Zielińskiinformation on the mechanical properties of NETs, and factors that may influence them. The fact that NETs are a particularly adhesive network of filaments poses a considerable technical challenge for contact probe methods and can limit advances involving either imaging or manipulation of NETs by AFM作者: AWRY 時(shí)間: 2025-3-28 21:37 作者: conflate 時(shí)間: 2025-3-28 23:01
1064-3745 ubleshooting and avoiding known pitfalls..Authoritative and cutting-edge,?.Atomic Force Microscopy: Methods and Protocols.?is useful for researchers at diffe978-1-4939-9407-6978-1-4939-8894-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 確保 時(shí)間: 2025-3-29 04:27
Investigating the Nanodomain Organization of Rhodopsin in Native Membranes by Atomic Force Microscopy visualize the packing of rhodopsin in native retinal membranes and the quantitative analysis of AFM images is beginning to reveal insights about the nanodomain organization of rhodopsin in the membrane. In this report, we outline procedures for imaging rhodopsin nanodomains by AFM and the quantita作者: grotto 時(shí)間: 2025-3-29 11:02
Single-Molecule Force Spectroscopy: Experiments, Analysis, and Simulationsng sample preparation, measurements, and analysis and interpretation of the resulting dynamic force spectrum?in terms of available theories. Next, we present the background for molecular dynamics (MD) simulations focusing on steered molecular dynamics (SMD) and the importance of bridging computation作者: antidepressant 時(shí)間: 2025-3-29 14:45 作者: photopsia 時(shí)間: 2025-3-29 16:01
Structural and Mechanical Characterization of Viruses with AFMprotein cages, ranging from imaging to spectroscopic methodologies devoted for extracting physical information, such as mechanical and electrostatic properties. We also explain how a convenient combination of AFM and fluorescence methodologies entails monitoring genome release from individual viral 作者: nocturia 時(shí)間: 2025-3-29 20:12 作者: scrutiny 時(shí)間: 2025-3-30 03:33 作者: Callus 時(shí)間: 2025-3-30 05:56 作者: BABY 時(shí)間: 2025-3-30 11:42 作者: 門閂 時(shí)間: 2025-3-30 13:06 作者: 仇恨 時(shí)間: 2025-3-30 16:53 作者: prosthesis 時(shí)間: 2025-3-30 20:47 作者: diskitis 時(shí)間: 2025-3-31 00:52
Studies in Fuzziness and Soft Computingbased single-molecule dynamic force spectroscopy represents one of the most powerful techniques available to directly investigate ligand-receptor recognition under physiological conditions without considerable disruption to cells. It provides important information for research on biological processe作者: 狂熱文化 時(shí)間: 2025-3-31 09:00
Real-Time Fuzzy Logic Controllers,. The quantification of mechanical resistance of cells and biomolecules using biophysical methods matured thanks to the development of nanotechnologies such as optical and magnetic tweezers, the biomembrane force probe, and atomic force microscopy (AFM). The quantitative nature of force spectroscopy作者: 老人病學(xué) 時(shí)間: 2025-3-31 09:56
https://doi.org/10.1007/978-3-319-26656-5e surface structure and in the force mode it can also be used to test the mechanical properties of the sample. AFM has been successfully applied to study the molecular mechanism of pore-forming proteins on model membranes. It gives information about both the structural reorganization of the membrane作者: ANIM 時(shí)間: 2025-3-31 14:36
Jér?me Fortin,Adam Kasperski,Pawe? Zielińskigen invasion and proliferation. Notwithstanding their protective role, NETs have also been linked to the development of a variety of disorders, including cardiovascular and autoimmune diseases. Since the first reports on NETs in 2004 it has been possible to image NETs by a variety of imaging techniq作者: 使糾纏 時(shí)間: 2025-3-31 19:16
Francesca A. Lisi,Umberto Straccias for following bacterial attachment to glass surfaces and provide protocols for coupling bacterial cells to AFM tips. Using single microbial cell force spectroscopy in physiological environment, we show methods to probe mechanical parameters and the dissociation of curliated . cells from fibronecti作者: 浪蕩子 時(shí)間: 2025-4-1 01:10
