標(biāo)題: Titlebook: Asthma; Methods and Protocol Magdalena M. Gorska Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license to Spri [打印本頁(yè)] 作者: 贊美 時(shí)間: 2025-3-21 19:03
書目名稱Asthma影響因子(影響力)
書目名稱Asthma影響因子(影響力)學(xué)科排名
書目名稱Asthma網(wǎng)絡(luò)公開(kāi)度
書目名稱Asthma網(wǎng)絡(luò)公開(kāi)度學(xué)科排名
書目名稱Asthma被引頻次
書目名稱Asthma被引頻次學(xué)科排名
書目名稱Asthma年度引用
書目名稱Asthma年度引用學(xué)科排名
書目名稱Asthma讀者反饋
書目名稱Asthma讀者反饋學(xué)科排名
作者: 水土 時(shí)間: 2025-3-21 21:58
Mouse Models of Respiratory Syncytial Virus Infection, a mixed immune response characterized by both Th1-type cells and Th2-type cells. This immune response, along with clinical features such as bronchiolitis, wheezing, and respiratory distress caused by RSV infection, presents similarly to many features of asthma and has led to an investigation into t作者: Intractable 時(shí)間: 2025-3-22 03:36
Modeling Asthma in Mice Using Rhinovirus Infection,his chapter outlines methods for the production of standardized human rhinovirus A1B and mouse infection. The chapter also describes methods to couple infections with allergen (ovalbumin and house dust mite) administrations. The production of the virus involves its amplification, purification, and c作者: 最初 時(shí)間: 2025-3-22 06:10
Mouse Model of Sendai Virus-Induced Lung Disease,nd genus . The virus was named after Sendai, city in Japan, where it was first isolated (Kuroya, Ishida, Yokohama Med Bull 4:217–233, 1953). Antigenically, SeV is closely related to human parainfluenza viruses 1 and 3. SeV is pneumotropic and naturally infects the respiratory tract of rodents. At th作者: 狗舍 時(shí)間: 2025-3-22 10:33
Ozone-Induced Models of Airway Hyperreactivity and Epithelial Injury,perreactivity when exposed to rodents in laboratory settings. Airway hyperreactivity is defined as an exaggerated acute obstructive response of the airways to one or more nonspecific stimuli. Lung permeability is a measure of barrier functions that separate internal and external environments to limi作者: 我正派 時(shí)間: 2025-3-22 13:12
A Novel Nonhuman Primate Model of Nonatopic Asthma, nonhuman primate closely reflect the complexity of human systems and provide an exceptional translational impact for the investigation of the mucosal immune changes in response to environmental exposures. This potential warrants the development of novel models that will clarify the interaction of r作者: 貪婪地吃 時(shí)間: 2025-3-22 20:26
Measurement of Airway Hyperresponsiveness in Mice,sm or new treatments of asthma, mice have been utilized to model the disease. Eosinophilic airway inflammation, allergen specific-IgE, and airway hyperresponsiveness have been characteristic features of allergic asthma (Drake et al. Pulm Ther 5:103–115, 2019). In mouse models, airway hyperresponsive作者: Fierce 時(shí)間: 2025-3-23 00:38 作者: Incise 時(shí)間: 2025-3-23 03:10 作者: 眉毛 時(shí)間: 2025-3-23 06:19 作者: 興奮過(guò)度 時(shí)間: 2025-3-23 13:09
Isolation, Ex Vivo Culture, and Stimulation of Tracheal and Nasal Chemosensory Cells,in the airway epithelium and are equipped with the machinery to generate prostaglandins and acetylcholine. Activation of innate type 2 lymphoid cells and dendritic cells triggered by brush cell-derived mediators skew the immune response in the airway to type 2 inflammation that underlies atopic dise作者: fidelity 時(shí)間: 2025-3-23 14:00
Detection, Isolation, and Functional Studies of Mouse Pulmonary Group 2 Innate Lymphoid Cells,2s, our method utilizes the surface markers CD127 and ST2 to classify a group of type 2 cytokine-producing ILC2s upon activation by the fungal allergen .. Here, we provide our protocol for the detection and isolation of a highly pure population of pulmonary mouse ILCs via flow cytometry and cell sor作者: 令人心醉 時(shí)間: 2025-3-23 21:04 作者: flex336 時(shí)間: 2025-3-24 02:15
Detection of Eosinophils in Tissue Sections by Immunohistochemistry,minantly reside in certain organs, such as the gastrointestinal tract. However, eosinophil numbers increase both in the peripheral blood and tissues during allergic inflammation, parasitic infestation, drug reactions, vasculitides, as well as certain hematopoietic neoplasms. Their presence in tissue作者: macrophage 時(shí)間: 2025-3-24 03:30
Targeting Eosinophils in Mouse Models of Asthma,sthma and models of allergic airway inflammation. Their role in the pathophysiology of asthma and other diseases can be studied using mouse models in which eosinophils are depleted. This review article focuses on two main approaches for depleting eosinophils in vivo, antibody-mediated and genetic mo作者: conceal 時(shí)間: 2025-3-24 08:58
Detection and Isolation of Airway Mast Cell Subsets in Mouse and Human, inflammation. While mast cell progenitors can be found in peripheral blood, phenotypically mature mast cells can only be found within peripheral tissues. Here, we describe optimized tissue digestion protocols for obtaining mast cells from the murine lung and from human sinus tissue. Following tissu作者: 挫敗 時(shí)間: 2025-3-24 12:10 作者: 精美食品 時(shí)間: 2025-3-24 15:12
Isolation and Analysis of Macrophage Subsets from the Mouse and Human Lung, homeostasis. During inflammation, these are joined by monocyte-derived recruited macrophages. Flow cytometry can be used to identify and purify lung macrophage subsets. Here, we describe methods for identifying and isolating macrophages from bronchoalveolar lavage and digested lung tissues from mou作者: 甜瓜 時(shí)間: 2025-3-24 21:50
Mass Cytometry Analysis of Whole Blood Response to an Allergen,An advantage over fluorescence cytometry is the relative lack of background, which provides excellent resolution for detection of phosphoproteins and quantification of cell signaling. We have applied mass cytometry to the analysis of whole blood staining after ex vivo stimulation with peanut allerge作者: 地名表 時(shí)間: 2025-3-24 23:29 作者: 關(guān)節(jié)炎 時(shí)間: 2025-3-25 04:36
Book 2022falls, and step-by-step, readily reproducible protocols...?..Authoritative and cutting-edge, .Asthma: Methods and Protocols. aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field. .作者: Migratory 時(shí)間: 2025-3-25 10:44
Formation and Early Growth of Business Websnism(s) by which a respiratory viral infection translates into postviral airway disease in mice. This chapter primarily describes the protocols we use to infect mice in vivo, assay viral replication, and assess outcomes in the lungs of the host.作者: brassy 時(shí)間: 2025-3-25 13:41
Towards a Theory of Business Web Growth,ity can be defined using in vitro exposures to airway epithelial cells. This chapter will focus on methods of generating O. and then exposing rodents and cultured epithelial cells in laboratory settings.作者: 蔑視 時(shí)間: 2025-3-25 18:58
https://doi.org/10.1007/978-1-4899-6707-7 of whole blood for mass cytometry analysis that is currently in use in multicenter clinical trials. The protocol can be easily adopted to analyze blood leukocytes in other diseases, including asthma.作者: 推測(cè) 時(shí)間: 2025-3-25 20:21 作者: 鋪?zhàn)?nbsp; 時(shí)間: 2025-3-26 02:18 作者: 高深莫測(cè) 時(shí)間: 2025-3-26 05:12
Mass Cytometry Analysis of Whole Blood Response to an Allergen, of whole blood for mass cytometry analysis that is currently in use in multicenter clinical trials. The protocol can be easily adopted to analyze blood leukocytes in other diseases, including asthma.作者: Crumple 時(shí)間: 2025-3-26 09:35
Towards a Theory of Business Web Growth,lending mechanistic insight into severe RSV infection. Here we present several materials and methods used for propagating and purifying RSV, infecting mice with RSV, and analyzing samples from RSV-infected mice.作者: Freeze 時(shí)間: 2025-3-26 13:43
Towards a Theory of Business Web Growth,n primate models of asthma can be spontaneous, induced, or experimentally manipulated by various exposures. Here we describe a model of exacerbation of airway hyperreactivity induced by exposure to an air pollutant, ozone, in a cohort of young adult asthmatic rhesus macaques.作者: Parley 時(shí)間: 2025-3-26 20:40 作者: Ablation 時(shí)間: 2025-3-26 21:48
Pre - Planetary Disks and Planet Formationcing Lin. population that lacks surface expression of canonical CRTH2 but expresses CD30 and TNFR2. Herein, we describe a detailed protocol for isolation, staining, and analysis of the conventional Lin.CRTH2.IL7Ra. and the non-conventional Lin.CD30.TNFR2. ILC2 populations.作者: 量被毀壞 時(shí)間: 2025-3-27 02:37 作者: 為敵 時(shí)間: 2025-3-27 08:03 作者: Assemble 時(shí)間: 2025-3-27 12:09
A Novel Nonhuman Primate Model of Nonatopic Asthma,n primate models of asthma can be spontaneous, induced, or experimentally manipulated by various exposures. Here we describe a model of exacerbation of airway hyperreactivity induced by exposure to an air pollutant, ozone, in a cohort of young adult asthmatic rhesus macaques.作者: 培養(yǎng) 時(shí)間: 2025-3-27 16:08
Human Bronchial Epithelial Cell Culture Models for Cigarette Smoke and Vaping Studies,ple who smoke, vape, or both. Here, we will describe methods to collect, expand, and culture human airway epithelial cells from endobronchial brushings and expose these cells cultured at the air–liquid interface to cigarette smoke or electronic cigarette vapor.作者: Hypomania 時(shí)間: 2025-3-27 18:59 作者: prostate-gland 時(shí)間: 2025-3-28 01:39 作者: menopause 時(shí)間: 2025-3-28 05:29
Towards a Theory of Business Web Growth,ods that are commonly used in mechanistic studies of asthma in mice. These methods include tracheotomy and bronchoalveolar lavage, cytospin and morphologic analysis of bronchoalveolar lavage cells, and lung harvest and digestion for generation of single-cell suspension.作者: entitle 時(shí)間: 2025-3-28 07:22
Formation and Early Growth of Business Websoncentration. In order to standardize the concentrated RV stock, a plaque assay on HeLa cells is outlined as a method of calibrating infectivity. Once the number of plaque-forming units is determined, the standardized virus is used for mouse infection.作者: temperate 時(shí)間: 2025-3-28 12:22
David N. Schramm,Katherine Freese stimulation of PCLSs with immune mediators such as pro-inflammatory tumor necrosis factor α (TNF-α). These models are useful to investigate mechanisms of virally induced asthma exacerbations and study modes of action and efficacy of antiviral and/or anti-inflammatory drugs.作者: 有危險(xiǎn) 時(shí)間: 2025-3-28 15:46
https://doi.org/10.1007/978-1-4615-1883-9e digestion, mast cells can be identified and sorted by flow cytometry using antibodies against defined surface markers. We also provide protocol for intracellular protease immunostaining, allowing for further characterization of mast cells.作者: figure 時(shí)間: 2025-3-28 22:31
1064-3745 ation advice from the experts.This volume details a collection of laboratory protocols to study asthma in mice and humans. Chapters cover animal models of asthma, methods to measure asthma-related molecules, protocols to detect, isolate, culture and stimulate cells that contribute to asthma in mice 作者: Osmosis 時(shí)間: 2025-3-29 02:24
Pancakes, Hot Gas and Microwave Distortionsary disease; mast cell-mediated airway contractility and inflammation; modulation of airway cells following pathogen exposure; and consequences of environmental toxicant exposure. Here we describe the generation of human precision-cut lung slices (hPCLS) and measurement of contraction and relaxation of small airways within the slices.作者: Archipelago 時(shí)間: 2025-3-29 06:31
https://doi.org/10.1007/978-94-009-3037-7n .. Here, we provide our protocol for the detection and isolation of a highly pure population of pulmonary mouse ILCs via flow cytometry and cell sorting. We also describe the methods for in vitro stimulation to assess the functionality of ILC2s.作者: 華而不實(shí) 時(shí)間: 2025-3-29 10:57
https://doi.org/10.1007/978-1-4615-1883-9which eosinophils are depleted. This review article focuses on two main approaches for depleting eosinophils in vivo, antibody-mediated and genetic models. Specific antibodies and genetic models are reviewed, along with their strengths and weaknesses.作者: GUMP 時(shí)間: 2025-3-29 13:30
Sarah Dunlop,Sandra Fraley,Lyn Beazleymacrophage subsets. Here, we describe methods for identifying and isolating macrophages from bronchoalveolar lavage and digested lung tissues from mouse and human. We also describe basic staining for flow cytometry analysis of different macrophage subsets.作者: 修剪過(guò)的樹(shù)籬 時(shí)間: 2025-3-29 18:30 作者: Generator 時(shí)間: 2025-3-29 21:03
Detection, Isolation, and Functional Studies of Mouse Pulmonary Group 2 Innate Lymphoid Cells,n .. Here, we provide our protocol for the detection and isolation of a highly pure population of pulmonary mouse ILCs via flow cytometry and cell sorting. We also describe the methods for in vitro stimulation to assess the functionality of ILC2s.作者: AVOW 時(shí)間: 2025-3-30 02:12
Targeting Eosinophils in Mouse Models of Asthma,which eosinophils are depleted. This review article focuses on two main approaches for depleting eosinophils in vivo, antibody-mediated and genetic models. Specific antibodies and genetic models are reviewed, along with their strengths and weaknesses.作者: 討好美人 時(shí)間: 2025-3-30 07:38
Isolation and Analysis of Macrophage Subsets from the Mouse and Human Lung,macrophage subsets. Here, we describe methods for identifying and isolating macrophages from bronchoalveolar lavage and digested lung tissues from mouse and human. We also describe basic staining for flow cytometry analysis of different macrophage subsets.作者: Observe 時(shí)間: 2025-3-30 11:48 作者: hyperuricemia 時(shí)間: 2025-3-30 13:37
Modeling Asthma in Mice Using Rhinovirus Infection,oncentration. In order to standardize the concentrated RV stock, a plaque assay on HeLa cells is outlined as a method of calibrating infectivity. Once the number of plaque-forming units is determined, the standardized virus is used for mouse infection.作者: DIKE 時(shí)間: 2025-3-30 19:07 作者: 較早 時(shí)間: 2025-3-30 22:17 作者: 雜役 時(shí)間: 2025-3-31 02:35 作者: 神圣不可 時(shí)間: 2025-3-31 06:15
Towards a Theory of Business Web Growth, a mixed immune response characterized by both Th1-type cells and Th2-type cells. This immune response, along with clinical features such as bronchiolitis, wheezing, and respiratory distress caused by RSV infection, presents similarly to many features of asthma and has led to an investigation into t作者: MAPLE 時(shí)間: 2025-3-31 09:55 作者: Polydipsia 時(shí)間: 2025-3-31 13:51 作者: Somber 時(shí)間: 2025-3-31 19:35
Towards a Theory of Business Web Growth,perreactivity when exposed to rodents in laboratory settings. Airway hyperreactivity is defined as an exaggerated acute obstructive response of the airways to one or more nonspecific stimuli. Lung permeability is a measure of barrier functions that separate internal and external environments to limi作者: Entropion 時(shí)間: 2025-3-31 21:59
Towards a Theory of Business Web Growth, nonhuman primate closely reflect the complexity of human systems and provide an exceptional translational impact for the investigation of the mucosal immune changes in response to environmental exposures. This potential warrants the development of novel models that will clarify the interaction of r作者: conference 時(shí)間: 2025-4-1 04:07
https://doi.org/10.1007/b138404sm or new treatments of asthma, mice have been utilized to model the disease. Eosinophilic airway inflammation, allergen specific-IgE, and airway hyperresponsiveness have been characteristic features of allergic asthma (Drake et al. Pulm Ther 5:103–115, 2019). In mouse models, airway hyperresponsive作者: 狼群 時(shí)間: 2025-4-1 06:52
Pancakes, Hot Gas and Microwave Distortionsntraction and relaxation; the pulmonary immune response in the context of inflammatory diseases of the lung like asthma and chronic obstructive pulmonary disease; mast cell-mediated airway contractility and inflammation; modulation of airway cells following pathogen exposure; and consequences of env作者: tackle 時(shí)間: 2025-4-1 10:35
David N. Schramm,Katherine Freesee we describe the infection of human PCLSs with the human influenza virus. We then provide methods to quantify the virus and reveal its localization within infected PCLSs and study consequences of infection, including cell death and production of cytokines, chemokine, and mucus. We also describe the作者: SUGAR 時(shí)間: 2025-4-1 15:27
