作者: Dawdle 時(shí)間: 2025-3-21 21:47
Fictional Worlds and the Moral Imaginationer with a randomized expression platform followed by in vivo selection and screening. Here, we describe an in vivo selection and screening technique to discover artificial riboswitches in .. . cells that is based on TEV protease–FRET substrate reporter system.作者: BROW 時(shí)間: 2025-3-22 02:03
Fictional Worlds and the Moral Imaginationescribe a library screening strategy for efficient creation of ON riboswitches of . of .. An ON riboswitch of . is then integrated with the . promoter, generating a hybrid device to achieve portable sequential OFF-and-ON gene regulation.作者: 祖?zhèn)?nbsp; 時(shí)間: 2025-3-22 05:08 作者: 極肥胖 時(shí)間: 2025-3-22 08:56 作者: 一夫一妻制 時(shí)間: 2025-3-22 16:14 作者: frivolous 時(shí)間: 2025-3-22 18:33
FRET-Based Optical Assay for Selection of Artificial Riboswitches,er with a randomized expression platform followed by in vivo selection and screening. Here, we describe an in vivo selection and screening technique to discover artificial riboswitches in .. . cells that is based on TEV protease–FRET substrate reporter system.作者: Exploit 時(shí)間: 2025-3-23 01:05
Portable Two-Way Riboswitches: Design and Engineering,escribe a library screening strategy for efficient creation of ON riboswitches of . of .. An ON riboswitch of . is then integrated with the . promoter, generating a hybrid device to achieve portable sequential OFF-and-ON gene regulation.作者: DAFT 時(shí)間: 2025-3-23 04:26
Engineering Protein-Responsive mRNA Switch in Mammalian Cells,ruct a genetic circuit. Insertion of a protein binding RNA sequence in the 5′ UTR of mRNA would allow for the generation of a protein-responsive RNA switch. Here we describe the design principle of the switch and methods for tuning and analyzing its translational activity in mammalian cells.作者: harmony 時(shí)間: 2025-3-23 08:29 作者: 團(tuán)結(jié) 時(shí)間: 2025-3-23 12:14
Engineered Riboswitch as a Gene-Regulatory Platform for Reducing Antibiotic Resistance,tch containing aptazyme is constructed in . to regulate the expression of β-lactamase through small molecule–aptamer interactions, which sharply reduces the antibiotic resistance of the engineered bacteria.作者: 停止償付 時(shí)間: 2025-3-23 16:33
Identification of RNA Aptamers Against Recombinant Proteins with a Hexa-Histidine Tag,rs against hexa-histidine-tagged proteins. For the efficient enrichment of higher affinity aptamers, the selection stringency should be gradually increased. Undesired RNA species that bind to affinity resins can be eliminated from the pool by using a negative selection step and alternating different types of resins.作者: 喚起 時(shí)間: 2025-3-23 21:32 作者: 說(shuō)不出 時(shí)間: 2025-3-23 23:23 作者: FUME 時(shí)間: 2025-3-24 06:07
Guanine-Tethered Antisense Oligonucleotides as Synthetic Riboregulators,y of using G-ASs to modulate RNA conformation may allow control of RNA function by inducing biologically important quadruplex structures. This approach to manipulate quadruplex structures using G-ASs may expand the strategies for regulating RNA structures and the functions of short oligonucleotide riboregulators.作者: craving 時(shí)間: 2025-3-24 08:37
Book 2014cules.. Artificial Riboswitches: Methods and Protocols. focuses on the state-of-the-art methods developed in recent years for creating artificial riboswitches, therefore this volume could be regarded as a collection of recipes for the gene circuit elements in synthetic biology and metabolic engineer作者: arsenal 時(shí)間: 2025-3-24 13:21 作者: vascular 時(shí)間: 2025-3-24 17:26
Charlie Kuppens,Catherine M. Robbhich is easily available for the design of other ligand-dependent riboswitches, by introducing a new region (a modulator sequence: MS) in addition to the two basic regions. A facile method for preparing arbitrary molecule-dependent riboswitches based on the foundational riboswitch is also presented.作者: Defraud 時(shí)間: 2025-3-24 21:04 作者: Fibrin 時(shí)間: 2025-3-25 00:11
,Development of Photoswitchable RNA Aptamer–Ligand Complexes,moiety that undergoes photoisomerization through light irradiation. Furthermore, we explain a procedure for surface plasmon resonance assay to detect photoswitchable association and dissociation of RNA–ligand complex on gold surface.作者: 冥想后 時(shí)間: 2025-3-25 06:37
Rational Design of Artificial ON-Riboswitches,hich is easily available for the design of other ligand-dependent riboswitches, by introducing a new region (a modulator sequence: MS) in addition to the two basic regions. A facile method for preparing arbitrary molecule-dependent riboswitches based on the foundational riboswitch is also presented.作者: 百靈鳥(niǎo) 時(shí)間: 2025-3-25 11:03
Construction of Ligand-Responsive MicroRNAs that Operate Through Inhibition of Drosha Processing,, the engineered microRNAs can be readily modified to target different sequences and to bind different ligands. Individual miRNAs also can be incorporated into the same transcript for tunable, multi-gene silencing.作者: 種子 時(shí)間: 2025-3-25 14:03
Plato’s , as an Ethical Performancers against hexa-histidine-tagged proteins. For the efficient enrichment of higher affinity aptamers, the selection stringency should be gradually increased. Undesired RNA species that bind to affinity resins can be eliminated from the pool by using a negative selection step and alternating different types of resins.作者: 陰謀小團(tuán)體 時(shí)間: 2025-3-25 16:50 作者: Vital-Signs 時(shí)間: 2025-3-25 22:03 作者: Robust 時(shí)間: 2025-3-26 02:22
Charlie Kuppens,Catherine M. Robby of using G-ASs to modulate RNA conformation may allow control of RNA function by inducing biologically important quadruplex structures. This approach to manipulate quadruplex structures using G-ASs may expand the strategies for regulating RNA structures and the functions of short oligonucleotide riboregulators.作者: callous 時(shí)間: 2025-3-26 04:19
Extrahuman Transcendence in Oscar Wilde’s quence with potential binding affinity from a pool of random sequences and designed a protocol for RNA 3D structure prediction. The proposed approach significantly reduces the RNA sequence search space, thus accelerating the experimental screening and selection of high-affinity aptamers.作者: 農(nóng)學(xué) 時(shí)間: 2025-3-26 11:45 作者: 腐爛 時(shí)間: 2025-3-26 14:49 作者: magnanimity 時(shí)間: 2025-3-26 20:33
Dual-Selection for Evolution of In Vivo Functional Aptazymes as Riboswitch Parts,phenylalanine and a hammerhead ribozyme. Because this method uses selection, it does not rely on sequence-specific design and thus should be generalizable for the generation of in vivo operational aptazymes that respond to any targeted molecules.作者: 滲透 時(shí)間: 2025-3-26 20:58 作者: Pageant 時(shí)間: 2025-3-27 03:54 作者: inscribe 時(shí)間: 2025-3-27 06:56
Extrahuman Transcendence in Oscar Wilde’s ools have a simple stem–loop structure. We present here a computational approach for designing a starting library of RNA sequences with increased formation of complex structural motifs and enhanced affinity to a desired target molecule. Our approach consists of two steps: (1) generation of RNA seque作者: 1分開(kāi) 時(shí)間: 2025-3-27 09:33 作者: Arable 時(shí)間: 2025-3-27 16:21 作者: yohimbine 時(shí)間: 2025-3-27 20:12
Plato’s , as an Ethical Performancedesigned and engineered using existing RNA aptamers that target proteins. Aptamers are generated via an iterative selection–amplification process, known as systematic evolution of ligands by exponential enrichment (SELEX). This chapter describes a SELEX procedure for the identification of RNA aptame作者: ULCER 時(shí)間: 2025-3-28 00:10
Fictional Worlds and the Moral Imagination their utility, methods are required to create custom riboswitches de novo or to modify existing riboswitches to suit specific experimental needs. This chapter describes such a method, which exploits fluorescence-activated cell sorting (FACS) to quickly and efficiently sort through large libraries o作者: 偽造 時(shí)間: 2025-3-28 06:01
Fictional Worlds and the Moral Imaginationseful tools to reprogram cellular behavior for different applications. A new synthetic riboswitch can be created by linking an in vitro-selected aptamer with a randomized expression platform followed by in vivo selection and screening. Here, we describe an in vivo selection and screening technique t作者: intellect 時(shí)間: 2025-3-28 09:08 作者: 同義聯(lián)想法 時(shí)間: 2025-3-28 11:23 作者: Deject 時(shí)間: 2025-3-28 16:47
Fictional Worlds and the Moral Imaginationibraries are constructed by randomizing the nucleotides that potentially comprise an expression platform and fused to the hybrid selection/screening marker .–.. Iterative ON and OFF selections are performed under appropriate conditions that favor the survival or the growth of the cells harboring the作者: choroid 時(shí)間: 2025-3-28 22:43 作者: 使成核 時(shí)間: 2025-3-28 22:53 作者: instill 時(shí)間: 2025-3-29 07:04 作者: TAG 時(shí)間: 2025-3-29 10:47
https://doi.org/10.1007/978-3-031-52026-6nthetic mRNA switches that modulate translation of a target gene of interest in response to an intracellular protein could be a key regulator to construct a genetic circuit. Insertion of a protein binding RNA sequence in the 5′ UTR of mRNA would allow for the generation of a protein-responsive RNA s作者: 圖畫(huà)文字 時(shí)間: 2025-3-29 12:48 作者: FUSC 時(shí)間: 2025-3-29 16:00
Culture, Recognition, and Poetry, for the control of gene expression in vivo, called artificial riboswitches. In this chapter, we describe how in vitro selection can be used to create an allosteric ribozyme that senses bacterial second messenger cyclic-di-GMP (c-di-GMP). A hammerhead ribozyme was joined to a natural c-di-GMP class 作者: 儀式 時(shí)間: 2025-3-29 20:37 作者: llibretto 時(shí)間: 2025-3-30 02:50
,‘Keynotes’ of Decadence, 1894–95,ors and diagnostic devices will become more important in the future to allow for noninvasive determination of protein, ion, or small molecule metabolite levels. In recent years synthetic RNA-based switches have been developed to act as signal transducers to convert a binding event of a small molecul作者: deciduous 時(shí)間: 2025-3-30 05:06
https://doi.org/10.1057/9780230504004c health problem. Enzymatic activation of antibiotics though the production of β-lactamase is one of the main mechanisms causing AR. Synthetic riboswitch containing aptazyme is constructed in . to regulate the expression of β-lactamase through small molecule–aptamer interactions, which sharply reduc作者: Indent 時(shí)間: 2025-3-30 09:14
Fictions of Disease in Early Modern Englandir utility in both fundamental genetic studies and as therapeutic agents. Rendering individual microRNAs responsive to endogenous or exogenously applied molecules (or ligands) can improve the stringency of silencing and can mediate autonomous control. This chapter describes the construction of ligan作者: 季雨 時(shí)間: 2025-3-30 16:04 作者: 陳腐的人 時(shí)間: 2025-3-30 18:44
Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/162721.jpg作者: 慷慨援助 時(shí)間: 2025-3-30 22:28 作者: 預(yù)測(cè) 時(shí)間: 2025-3-31 04:34
978-1-4939-6330-0Springer Science+Business Media New York 2014作者: Outmoded 時(shí)間: 2025-3-31 05:29
In Vitro Selection of RNA Aptamers for a Small-Molecule Dye, that can bind strongly and selectively to their target are feasible to be used for obtaining more potent artificial riboswitches. In this chapter, we describe tips and notes for in vitro selection of RNA aptamers targeting synthetic small molecules.作者: goodwill 時(shí)間: 2025-3-31 10:58 作者: 可以任性 時(shí)間: 2025-3-31 14:25
Charlie Kuppens,Catherine M. Robbompleted only by liquid handling, facilitating the parallel and continuous operations of multiple selection projects. This automation-liable platform for genetic selection of functional switches has potential applications for development of RNA-based biosensors, expression controllers, and their int作者: 沒(méi)有準(zhǔn)備 時(shí)間: 2025-3-31 17:45 作者: 消散 時(shí)間: 2025-4-1 00:45 作者: MAZE 時(shí)間: 2025-4-1 04:33
Generation of Orthogonally Selective Bacterial Riboswitches by Targeted Mutagenesis and In Vivo Scrnd library to identify functional riboswitch–ligand combinations. Promising riboswitch–ligand pairs are then further characterized both in vivo and in vitro. Using this method, a series of artificial riboswitches can be generated that are versatile synthetic biology tools for use in protein producti作者: 退出可食用 時(shí)間: 2025-4-1 07:37
Nucleotide Kinase-Based Selection System for Genetic Switches,ompleted only by liquid handling, facilitating the parallel and continuous operations of multiple selection projects. This automation-liable platform for genetic selection of functional switches has potential applications for development of RNA-based biosensors, expression controllers, and their int作者: 神圣不可 時(shí)間: 2025-4-1 12:30
,Measuring Riboswitch Activity In Vitro and in Artificial Cells with Purified Transcription–Translatd system can be easily reconstituted within compartments to build artificial, cellular mimics with sensing capability, protocols are provided for building sense-response systems within water-in-oil emulsion compartments and lipid vesicles. Only standard laboratory equipment and commercially availabl作者: 舊石器 時(shí)間: 2025-4-1 15:41 作者: 反感 時(shí)間: 2025-4-1 21:42
1064-3745 reproducible protocols, and notes on troubleshooting and avoiding known pitfalls..Authoritative and easily accessible, .Artificial Riboswitches: Methods and Protocols. seeks to serve not only bioengineers who 978-1-4939-6330-0978-1-62703-755-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 采納 時(shí)間: 2025-4-1 23:15 作者: 大方不好 時(shí)間: 2025-4-2 04:19
