作者: 積習(xí)已深 時(shí)間: 2025-3-21 21:36 作者: 現(xiàn)實(shí) 時(shí)間: 2025-3-22 02:07
Characterization of Innate Immune Signalings Stimulated by Ligands for Pattern Recognition Receptorligands and for detection of innate immune response, that is, IFN and proinflammatory cytokine induction, as assessed by luciferase assay, quantitative RT-PCR (qRT-PCR), and enzyme-linked immunosorbent assay.作者: peak-flow 時(shí)間: 2025-3-22 05:46
In Vivo Cell Transfer Assay to Detect Autoreactive T Cell Subsets,l lymphoid organs. These .CD4 T cells not only induce a variety of autoantibodies but also promote the final differentiation of CD8 T cells into cytotoxic T lymphocytes, resulting in a pathology identical to SLE. We needed to develop a new methodology to isolate this subpopulation of .CD4 T cells. H作者: 微生物 時(shí)間: 2025-3-22 09:32
Bayesian Systems-Based Genetic Association Analysis with Effect Strength Estimation and Omic Wide Ingineering to semantic publication of the results. We overview the construction, visualization, and interpretation of complex hypotheses related to the structural analysis of relevance. Furthermore, we illustrate the use of a dependency model-based relevance measure, which takes into account the str作者: armistice 時(shí)間: 2025-3-22 15:18
Label-Free Imaging of Adipogenesis by Coherent Anti-Stokes Raman Scattering Microscopy,longitudinal analyses of live cells and tissues. CARS provides a coherently emitted signal that is much stronger than the spontaneous Raman scattering. The anti-Stokes signal is blue shifted from the incident wavelength, thus reducing the non-vibrational background present in most biological materia作者: Coma704 時(shí)間: 2025-3-22 19:59
Essentials of Business Analytics, polychromatic flow cytometry is the most common and reliable tool to monitor T cell functions..We describe here the method for detection of immune-exhaustion status of CD8 T cells from human peripheral blood mononuclear cells (PBMCs). By stimulation of PBMCs with PMA/ionomycin for 6 h, more than 1作者: 消音器 時(shí)間: 2025-3-22 22:58
Statistical Methods: Regression Analysisligands and for detection of innate immune response, that is, IFN and proinflammatory cytokine induction, as assessed by luciferase assay, quantitative RT-PCR (qRT-PCR), and enzyme-linked immunosorbent assay.作者: separate 時(shí)間: 2025-3-23 03:24
Antithrombotic and Antiplatelet Therapyl lymphoid organs. These .CD4 T cells not only induce a variety of autoantibodies but also promote the final differentiation of CD8 T cells into cytotoxic T lymphocytes, resulting in a pathology identical to SLE. We needed to develop a new methodology to isolate this subpopulation of .CD4 T cells. H作者: 束縛 時(shí)間: 2025-3-23 06:26
https://doi.org/10.1007/978-1-4471-3708-5ngineering to semantic publication of the results. We overview the construction, visualization, and interpretation of complex hypotheses related to the structural analysis of relevance. Furthermore, we illustrate the use of a dependency model-based relevance measure, which takes into account the str作者: dilute 時(shí)間: 2025-3-23 13:12 作者: ALOFT 時(shí)間: 2025-3-23 16:28 作者: RUPT 時(shí)間: 2025-3-23 18:39
Peeyush Taori,Hemanth Kumar Dasararajuritical roles not only in normal bone homeostasis (remodeling) but also in the pathogenesis of bone-destructive disorders such as osteoporosis and rheumatoid arthritis. However, how the activity of mature osteoclasts is regulated in vivo remains unclear. To answer this question, we recently develope作者: 繼承人 時(shí)間: 2025-3-24 01:48 作者: 原告 時(shí)間: 2025-3-24 03:30
Statistical Methods: Regression Analysislosely related to the pathogenesis of autoimmune and inflammatory diseases. Characterization of the innate immune signalings may provide a pathophysiological insight for better understanding of human diseases. Nucleic acid-mediated activation of pattern recognition receptors triggers the activation 作者: Explicate 時(shí)間: 2025-3-24 07:07 作者: 慷慨援助 時(shí)間: 2025-3-24 10:40
Chronic Pain: Pathophysiology and Mechanismsire. These mice have been extensively used as tools to circumvent the low precursor frequency of naturally occurring endogenous T cells. As such, they have been valuable to study pathways of T-cell development in the thymus. In addition, these mice can also be considered as a valuable source of naiv作者: ACME 時(shí)間: 2025-3-24 18:31 作者: 喃喃訴苦 時(shí)間: 2025-3-24 20:20 作者: ineluctable 時(shí)間: 2025-3-25 00:24
https://doi.org/10.1007/978-90-481-9142-0 These innate-like lymphocytes express semi-invariant T cell receptors, but exert diverse functions and thus are involved in various types of immune responses. As iNKT cells and MAIT cells are abundant in human peripheral blood, these cells may hold important physiological roles, and thus it is desi作者: 混合,攙雜 時(shí)間: 2025-3-25 04:15
Ted Eastlund,Martell K. Winters induce disease in normal mice not having a genetic disposition to autoimmunity. We describe here a novel and completely reproducible experimental technique that can induce systemic autoimmunity or systemic lupus erythematosus (SLE) in mice otherwise not prone to spontaneous autoimmune disease. This作者: MILL 時(shí)間: 2025-3-25 09:19
Storage, Processing and Preservationonnective tissue disorder that affects particularly the skin. SSc is characterized by vasculopathy, immune disturbances, and fibrosis. Expression of each of the three pathologic features varies among SSc patients leading to disease heterogeneity and variable organ manifestations. Several animal mode作者: GOAT 時(shí)間: 2025-3-25 12:56
https://doi.org/10.1007/978-90-481-9142-0ug treatment, are urgently needed to select adequate drugs for patients. Gene mutation status, protein expression levels in a biopsy, and serum proteins are often used as biomarkers. One of the methods to screen for protein biomarkers involves quantitative proteomic approaches using mass spectrometr作者: 澄清 時(shí)間: 2025-3-25 16:36
Dynamic and open population modelsg on their abnormal quantitative phenotypes in relation to the loss of self-tolerance will lead to the identification of polymorphic genes that play pivotal roles in the genetic predisposition to autoimmunity. In this chapter, we first overview the basic concepts in the statistical genetics and then作者: Abnormal 時(shí)間: 2025-3-25 22:39 作者: 壕溝 時(shí)間: 2025-3-26 01:50 作者: 徹底明白 時(shí)間: 2025-3-26 06:59
D. L. Borchers,S. T. Buckland,W. Zucchinial stromal cells (MSCs) offer an attractive and convenient alternative to conventional fixative based lipid dyes such as Oil Red O and Sudan Red, fluorescent labels such as LipidTOX, and more indirect methods such as qRT-PCR analyses of specific adipocyte differentiation markers such as peroxisome P作者: Bronchial-Tubes 時(shí)間: 2025-3-26 12:08
https://doi.org/10.1007/978-1-4939-0404-4autoimmune disease; microRNAs; mouse models; rheumatoid arthritis; transgene techniques作者: 大罵 時(shí)間: 2025-3-26 15:09
978-1-4939-5393-6Springer Science+Business Media New York 2014作者: obsolete 時(shí)間: 2025-3-26 17:22
Shunichi ShiozawaIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: 釘牢 時(shí)間: 2025-3-27 00:25 作者: Cumulus 時(shí)間: 2025-3-27 02:51 作者: 豐富 時(shí)間: 2025-3-27 08:22 作者: 衍生 時(shí)間: 2025-3-27 12:35 作者: LEVY 時(shí)間: 2025-3-27 15:35
Intravital Multiphoton Microscopy for Dissecting Cellular Dynamics in Arthritic Inflammation and Boritical roles not only in normal bone homeostasis (remodeling) but also in the pathogenesis of bone-destructive disorders such as osteoporosis and rheumatoid arthritis. However, how the activity of mature osteoclasts is regulated in vivo remains unclear. To answer this question, we recently develope作者: cluster 時(shí)間: 2025-3-27 21:18
Monitoring Multifunctionality of Immune-Exhausted CD8 T Cells in Cancer Patients,es not always imply that cancers and/or pathogens are efficiently eliminated in the body. Concerning this point, the recent studies suggest the concept of immune exhaustion of CD8 T cells, characterized by their decreased production of IL-2, TNFα, and IFNγ even after antigen stimulation. Thus, conti作者: LAPSE 時(shí)間: 2025-3-28 00:53 作者: Armory 時(shí)間: 2025-3-28 05:30
Principles for the Use of In Vivo Transgene Techniques: Overview and an Introductory Practical Guidtional transgenic mice have the limitation that constitutive expression of a transgene from the embryonic stage may affect the normal development of the mice or cause compensating effects. To overcome these disadvantages, tetracycline-controlled transgenic mice, which can express target gene product作者: Cholesterol 時(shí)間: 2025-3-28 09:32
Unraveling Autoimmunity with the Adoptive Transfer of T Cells from TCR-Transgenic Mice,ire. These mice have been extensively used as tools to circumvent the low precursor frequency of naturally occurring endogenous T cells. As such, they have been valuable to study pathways of T-cell development in the thymus. In addition, these mice can also be considered as a valuable source of naiv作者: Thyroid-Gland 時(shí)間: 2025-3-28 10:34
In Vivo Cell Transfer Assay to Detect Autoreactive T Cell Subsets,are less widely employed. In our studies on systemic lupus erythematosus (SLE), we have identified a novel T cell subtype termed “autoantibody-inducing CD4 T cells” (.CD4 T cell) that is responsible for the development of autoimmunity. In order to identify and isolate these cells, we developed a new作者: Optimum 時(shí)間: 2025-3-28 18:20 作者: Sleep-Paralysis 時(shí)間: 2025-3-28 19:22 作者: Trypsin 時(shí)間: 2025-3-29 02:57
Induction of , Autoimmune Disease in Normal Mice upon Repeated Immunization with Antigen, induce disease in normal mice not having a genetic disposition to autoimmunity. We describe here a novel and completely reproducible experimental technique that can induce systemic autoimmunity or systemic lupus erythematosus (SLE) in mice otherwise not prone to spontaneous autoimmune disease. This作者: Somber 時(shí)間: 2025-3-29 04:39 作者: Adornment 時(shí)間: 2025-3-29 07:33
Screening for Novel Serum Biomarker for Monitoring Disease Activity in Rheumatoid Arthritis Using iug treatment, are urgently needed to select adequate drugs for patients. Gene mutation status, protein expression levels in a biopsy, and serum proteins are often used as biomarkers. One of the methods to screen for protein biomarkers involves quantitative proteomic approaches using mass spectrometr作者: scotoma 時(shí)間: 2025-3-29 13:34
Genome-Wide Genetic Study in Autoimmune Disease-Prone Mice,g on their abnormal quantitative phenotypes in relation to the loss of self-tolerance will lead to the identification of polymorphic genes that play pivotal roles in the genetic predisposition to autoimmunity. In this chapter, we first overview the basic concepts in the statistical genetics and then作者: 間諜活動(dòng) 時(shí)間: 2025-3-29 17:53 作者: Suggestions 時(shí)間: 2025-3-29 22:19
Sample Processing, Protocol, and Statistical Analysis of the Time-of-Flight Secondary Ion Mass Speced to distinguish healthy and osteoarthritic human cartilage. This chapter discusses ToF-SIMS principle and instrumentation including the three modes of analysis in ToF-SIMS. ToF-SIMS sets certain requirements for the samples to be analyzed; for example, the samples have to be vacuum compatible. Acc作者: 閹割 時(shí)間: 2025-3-30 00:54
Label-Free Imaging of Adipogenesis by Coherent Anti-Stokes Raman Scattering Microscopy,al stromal cells (MSCs) offer an attractive and convenient alternative to conventional fixative based lipid dyes such as Oil Red O and Sudan Red, fluorescent labels such as LipidTOX, and more indirect methods such as qRT-PCR analyses of specific adipocyte differentiation markers such as peroxisome P作者: 神秘 時(shí)間: 2025-3-30 07:43
Book 2014Latest edition arthritis and related conditions. A compendium of leaders in the field contribute chapters that cover practical research methods such as the intravital multiphoton microscopy technique, techniques for evaluating exhausted CD8 T cell and for studying nucleic acid sensors and their effects, methods f作者: abracadabra 時(shí)間: 2025-3-30 09:33
1064-3745 ation advice from the experts.Arthritis Research: Methods and Protocols, Second Edition .expands upon the. .first edition to present new and current techniques for the research of arthritis and related conditions. A compendium of leaders in the field contribute chapters that cover practical research作者: Rejuvenate 時(shí)間: 2025-3-30 12:47 作者: TEM 時(shí)間: 2025-3-30 19:38 作者: Ischemia 時(shí)間: 2025-3-30 22:15
Dynamic and open population modelsivotal roles in the genetic predisposition to autoimmunity. In this chapter, we first overview the basic concepts in the statistical genetics and then provide guides to genotyping microsatellite DNA markers and to quantitative trait loci mapping using a MAPMAKER program.作者: 虛弱的神經(jīng) 時(shí)間: 2025-3-31 03:20 作者: Stable-Angina 時(shí)間: 2025-3-31 05:30
Induction of , Autoimmune Disease in Normal Mice upon Repeated Immunization with Antigen,hnique that can induce systemic autoimmunity or systemic lupus erythematosus (SLE) in mice otherwise not prone to spontaneous autoimmune disease. This protocol involves the repeated immunization of mice with the same antigen. This rather simple technique enables us to perform exact and quantitative in vivo animal experiments with great accuracy.作者: Biguanides 時(shí)間: 2025-3-31 09:12
Genome-Wide Genetic Study in Autoimmune Disease-Prone Mice,ivotal roles in the genetic predisposition to autoimmunity. In this chapter, we first overview the basic concepts in the statistical genetics and then provide guides to genotyping microsatellite DNA markers and to quantitative trait loci mapping using a MAPMAKER program.作者: Obsequious 時(shí)間: 2025-3-31 13:24 作者: 解凍 時(shí)間: 2025-3-31 21:07 作者: 鐵砧 時(shí)間: 2025-4-1 00:41 作者: 喚起 時(shí)間: 2025-4-1 04:20
Mouse Model of Experimental Dermal Fibrosis: The Bleomycin-Induced Dermal Fibrosis,ls of SSc are available; however, some models display inflammation followed by fibrosis, whether some others primarily mimic autonomous fibroblast activation. Here, we describe the mouse model of bleomycin-induced dermal fibrosis, which mimics early and inflammatory stages of SSc, and is widely used in SSc research.作者: nocturia 時(shí)間: 2025-4-1 08:48
D. L. Borchers,S. T. Buckland,W. Zucchiniix for the ToF-SIMS are presented. Multivariate analysis of the ToF-SIMS data and the necessary data preprocessing steps (peak selection, data normalization, mean-centering, and scaling and transformation) are discussed in this chapter.作者: 搖晃 時(shí)間: 2025-4-1 10:32
Sample Processing, Protocol, and Statistical Analysis of the Time-of-Flight Secondary Ion Mass Specix for the ToF-SIMS are presented. Multivariate analysis of the ToF-SIMS data and the necessary data preprocessing steps (peak selection, data normalization, mean-centering, and scaling and transformation) are discussed in this chapter.作者: Aromatic 時(shí)間: 2025-4-1 15:55
