標(biāo)題: Titlebook: Archaea; Methods and Protocol Sébastien Ferreira-Cerca Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license t [打印本頁] 作者: Buren 時間: 2025-3-21 19:44
書目名稱Archaea影響因子(影響力)
書目名稱Archaea影響因子(影響力)學(xué)科排名
書目名稱Archaea網(wǎng)絡(luò)公開度
書目名稱Archaea網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Archaea被引頻次
書目名稱Archaea被引頻次學(xué)科排名
書目名稱Archaea年度引用
書目名稱Archaea年度引用學(xué)科排名
書目名稱Archaea讀者反饋
書目名稱Archaea讀者反饋學(xué)科排名
作者: 諄諄教誨 時間: 2025-3-21 23:44
Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/161163.jpg作者: Haphazard 時間: 2025-3-22 03:42
Ausgangslage und Forschungsdefizitgh in biology. With the methodologies available at this time, they have shown that a single molecule, the 16S ribosomal RNA, could reveal the global organization of the living world. Later on, mining archaeal genomes led to major discoveries in archaeal molecular biology, providing a third model for作者: gene-therapy 時間: 2025-3-22 06:18
John F. Stevenson,John C. Norcrosslable model archaeal genetic systems. We consider the limitations and barriers involved in genetically modifying different archaeal species, the techniques and breakthroughs that have contributed to their tractability, and potential areas for future development.作者: calumniate 時間: 2025-3-22 10:58 作者: SPURN 時間: 2025-3-22 15:02 作者: 偽造者 時間: 2025-3-22 18:47 作者: Minikin 時間: 2025-3-22 21:36 作者: 棲息地 時間: 2025-3-23 02:21
F. Schmitten,K.-H. Schepers,A. Festerlingily of . encompasses mesophilic and thermophilic hydrogenotrophic species. Mesophilic species are found in various natural and anthropogenic environments (e.g., are associated with the microbiome in animals and humans). Thermophilic species can be found in thermally active bogs and warm sulfuric spr作者: Missile 時間: 2025-3-23 07:55
Patricia A. Barton-Gade,Eli V. Olsenection system. . is a crenarchaeal model organism that grows optimally at 75?°C and a pH of 3. These extreme growth conditions cause some difficulties in developing a genetic system. With continuous efforts, versatile genetic tools have been developed for different species from the order of Sulfolob作者: 暖昧關(guān)系 時間: 2025-3-23 11:30
K. Lundstr?m,I. Hansson,G. Bj?rstorp organisms across all three domains of life. Gene function studies in Archaea, particularly for those flourishing in inhospitable natural environments that are anaerobic, usually hot, and acidic, have been a great challenge; however, this situation was recently overturned with the increasing availab作者: deface 時間: 2025-3-23 15:02 作者: 驚奇 時間: 2025-3-23 18:16 作者: flammable 時間: 2025-3-24 00:57
Raymond Bisdorff,Luis C. Dias,Marc Pirlotm, most recently by applying genome-, transcriptome-, proteome-, and metabolome-scale methods. This chapter describes seminal contributions that elaborate on the study of archaeal biology at the systems level.作者: Diverticulitis 時間: 2025-3-24 05:21 作者: 隼鷹 時間: 2025-3-24 09:42 作者: 數(shù)量 時間: 2025-3-24 14:28 作者: contradict 時間: 2025-3-24 16:25 作者: 俗艷 時間: 2025-3-24 21:41
Multi-Dimensional Preference Models,nation of protein synthesis and degradation, referred collectively as protein turnover. Different proteomics techniques have been developed to determine the whole proteome turnover of a cell, but very few have been applied to archaea. In this chapter we describe a heavy isotope multilabeling method 作者: 殺人 時間: 2025-3-25 00:47
Progress and Challenges in Archaeal Genetic Manipulationlable model archaeal genetic systems. We consider the limitations and barriers involved in genetically modifying different archaeal species, the techniques and breakthroughs that have contributed to their tractability, and potential areas for future development.作者: 整頓 時間: 2025-3-25 05:24 作者: COW 時間: 2025-3-25 10:31 作者: Hippocampus 時間: 2025-3-25 15:02 作者: pulmonary-edema 時間: 2025-3-25 18:37
Archaea978-1-0716-2445-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: BILE 時間: 2025-3-25 21:50
John F. Stevenson,John C. Norcrosslable model archaeal genetic systems. We consider the limitations and barriers involved in genetically modifying different archaeal species, the techniques and breakthroughs that have contributed to their tractability, and potential areas for future development.作者: sparse 時間: 2025-3-26 03:46 作者: 光亮 時間: 2025-3-26 06:19
https://doi.org/10.1007/978-1-4615-1593-7rtion mutant library in this crenarchaeal model. We envision that the traditional transposon-based forward mutagenesis screening paired with next generation sequencing will greatly speed up the exploration of archaeal genomic features.作者: Ornithologist 時間: 2025-3-26 10:51
https://doi.org/10.1007/0-387-31099-1ng library from culture harvesting, footprint isolation via ultracentrifugation, gel-based size fractionation, and footprint sequencing for a model halophilic archaeon, .. This approach has revealed the first global view of translation in the archaea.作者: 大笑 時間: 2025-3-26 12:51
Transposon Insertion Mutagenesis in Hyperthermophilic Crenarchaeon rtion mutant library in this crenarchaeal model. We envision that the traditional transposon-based forward mutagenesis screening paired with next generation sequencing will greatly speed up the exploration of archaeal genomic features.作者: minimal 時間: 2025-3-26 17:06
Conversion of Ribosome-Protected mRNA to DNA and Deep Sequencing for Ribosome Profiling in ng library from culture harvesting, footprint isolation via ultracentrifugation, gel-based size fractionation, and footprint sequencing for a model halophilic archaeon, .. This approach has revealed the first global view of translation in the archaea.作者: 不能平靜 時間: 2025-3-26 21:25
Genetic Methods and Construction of Chromosomal Mutations in Methanogenic Archaearoduction of the . gene on a plasmid leads to a selectable loss of 8-ADP resistance after a homologous recombination event (pop-in). A subsequent pop-out event restores the 8-ADP resistance and can generate chromosomal mutants with frequencies of about 50%.作者: Overdose 時間: 2025-3-27 03:15 作者: 糾纏,纏繞 時間: 2025-3-27 08:08
Proteome Turnover Analysis in , by a Heavy Isotope Multilabeling Approachthat allowed the successful analysis of relative protein synthesis and degradation rates on the proteome scale of the halophilic archaeon .. This method combines .N and .C isotope metabolic labeling with high-resolution mass spectrometry and data analysis tools (QuPE web-based platform) and could be applied to different archaea.作者: LINE 時間: 2025-3-27 11:46
Assessment for conservation and protection,article gives a step-by-step introduction on how to use the implemented system for any gene of interest in . .. The concept of CRISPRi described here for . can be transferred to any other archaeon, that is genetically tractable and has an endogenous CRISPR-Cas I systems.作者: habitat 時間: 2025-3-27 15:29
F. Schmitten,K.-H. Schepers,A. Festerlingonal DNA transfer from . to . ΔH with shuttle-vector plasmid DNA, which allows the genetic manipulation of this microbe, and provides a basis for the development of further genetic methods for this and potentially other representatives of Methanobacteriales.作者: 事情 時間: 2025-3-27 20:03 作者: ENDOW 時間: 2025-3-27 23:08 作者: nugatory 時間: 2025-3-28 05:03 作者: 注射器 時間: 2025-3-28 08:32 作者: BILK 時間: 2025-3-28 10:37 作者: FAWN 時間: 2025-3-28 16:06 作者: sulcus 時間: 2025-3-28 20:58
Book 2022 and step-by-step, readily reproducible protocols..Authoritative and cutting-edge, .Archaea: Methods and Protocols. aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field..作者: 褲子 時間: 2025-3-28 23:39
Book 2022 access to the molecular and cellular biology characterization of these fascinating organisms. Chapters are divided into five parts detailing available genetic tools, molecular and cellular biology methods, strategies to study the ecophysiology of archaea, and classroom protocol. Each main thematic 作者: 木訥 時間: 2025-3-29 04:29
Current Topics in Veterinary Medicineroduction of the . gene on a plasmid leads to a selectable loss of 8-ADP resistance after a homologous recombination event (pop-in). A subsequent pop-out event restores the 8-ADP resistance and can generate chromosomal mutants with frequencies of about 50%.作者: Modicum 時間: 2025-3-29 10:00
Patricia A. Barton-Gade,Eli V. Olsenales. In this chapter, we describe the methods for the available genetic tools in . including a (1) transformation method, (2) pop in/pop out strategy to generate markerless deletion mutants and (3) a plasmid-based expression system.作者: GUILT 時間: 2025-3-29 14:49
Multi-Dimensional Preference Models,that allowed the successful analysis of relative protein synthesis and degradation rates on the proteome scale of the halophilic archaeon .. This method combines .N and .C isotope metabolic labeling with high-resolution mass spectrometry and data analysis tools (QuPE web-based platform) and could be applied to different archaea.作者: paragon 時間: 2025-3-29 18:56 作者: HARP 時間: 2025-3-29 21:14
Assessment for conservation and protection,n into the genome or through retention of autonomously replicating plasmids. The robust genetic system developed in . offers a variety of selectable and counterselectable markers for complex, accurate and iterative genetic manipulations offering greater flexibility to probe gene function in vivo.作者: LATHE 時間: 2025-3-30 02:24
https://doi.org/10.1007/0-387-31099-1s of only a few have been characterized experimentally. Here, we describe how to build sRNA sequencing libraries using size-selected total RNA in the model archaeon, ., to provide a tool to further characterize sRNAs in archaea.作者: sulcus 時間: 2025-3-30 04:13 作者: 逃避現(xiàn)實 時間: 2025-3-30 08:15 作者: 狂怒 時間: 2025-3-30 13:27 作者: Alopecia-Areata 時間: 2025-3-30 19:11 作者: Panther 時間: 2025-3-30 22:16 作者: Adulterate 時間: 2025-3-31 02:43
ChIP-Seq Occupancy Mapping of the Archaeal Transcription Machineryof many established archaeal model organisms necessitates further adaptations. This chapter describes a version of ChIP-seq adapted for the basal transcription machinery of thermophilic archaea and some modifications to the data analysis.作者: 匍匐 時間: 2025-3-31 05:38 作者: 解凍 時間: 2025-3-31 11:56 作者: 說明 時間: 2025-3-31 17:15
https://doi.org/10.1007/978-1-4615-1593-7es found in the CRISPR arrays, effectively suppress unspecific DNA and RNA activities of the CRISPR-Cas complexes. Here we describe the use of endogenous type III CRISPR-Cas systems in two model organisms of Crenarchaeota, . and ..作者: Ingest 時間: 2025-3-31 20:54
Alexis Tsoukiàs,Herimandimbiniaina Ralijaonaof many established archaeal model organisms necessitates further adaptations. This chapter describes a version of ChIP-seq adapted for the basal transcription machinery of thermophilic archaea and some modifications to the data analysis.作者: Acetaminophen 時間: 2025-3-31 23:32
1064-3745 eshooting and known pitfalls, and step-by-step, readily reproducible protocols..Authoritative and cutting-edge, .Archaea: Methods and Protocols. aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field..978-1-0716-2447-0978-1-0716-2445-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 狂亂 時間: 2025-4-1 04:32
Archaea: A Goldmine for Molecular Biologists and Evolutionists,gh in biology. With the methodologies available at this time, they have shown that a single molecule, the 16S ribosomal RNA, could reveal the global organization of the living world. Later on, mining archaeal genomes led to major discoveries in archaeal molecular biology, providing a third model for
