標題: Titlebook: Arbuscular Mycorrhizal Fungi; Methods and Protocol Nuria Ferrol,Luisa Lanfranco Book 2020 Springer Science+Business Media, LLC, part of Spr [打印本頁] 作者: 撕成碎片 時間: 2025-3-21 16:43
書目名稱Arbuscular Mycorrhizal Fungi影響因子(影響力)
書目名稱Arbuscular Mycorrhizal Fungi影響因子(影響力)學科排名
書目名稱Arbuscular Mycorrhizal Fungi網(wǎng)絡公開度
書目名稱Arbuscular Mycorrhizal Fungi網(wǎng)絡公開度學科排名
書目名稱Arbuscular Mycorrhizal Fungi被引頻次
書目名稱Arbuscular Mycorrhizal Fungi被引頻次學科排名
書目名稱Arbuscular Mycorrhizal Fungi年度引用
書目名稱Arbuscular Mycorrhizal Fungi年度引用學科排名
書目名稱Arbuscular Mycorrhizal Fungi讀者反饋
書目名稱Arbuscular Mycorrhizal Fungi讀者反饋學科排名
作者: 不透明性 時間: 2025-3-21 20:43
Guardianship and Conservatorship,l images of both overall intraradical fungal colonization pattern and fine morphology, in AM root sections of .. The procedure is based on tissue clearing, fluorescent staining of fungal cell walls with fluorescein isothiocyanate-conjugated wheat germ agglutinin (FITC-WGA), and the combined counters作者: 健談的人 時間: 2025-3-22 00:27
Competence to Consent to Treatment,data generated by the sequencing run, with several crucial steps (e.g., library trimming, reads mapping, normalization, and differential expression calculation) which can strongly affect the final results. Here, we propose a standard workflow for de novo transcriptome assembly and differential expre作者: Perennial長期的 時間: 2025-3-22 06:17
A Whole-Plant Culture Method to Study Structural and Functional Traits of Extraradical Mycelium,as and densities of ERM showed wide ranges of variation, and its length ranged from 9.7?±?2.0 to 48.8?±?9.9?m per plant, depending on host and AMF identity. Across the different plant-AMF combinations tested, the whole-plant system produced 2.0?±?0.6 to 5.3?±?0.3?mg of ERM fresh biomass per plant pe作者: Console 時間: 2025-3-22 09:15
Fluorescent Staining of Arbuscular Mycorrhizal Structures Using Wheat Germ Agglutinin (WGA) and Prol images of both overall intraradical fungal colonization pattern and fine morphology, in AM root sections of .. The procedure is based on tissue clearing, fluorescent staining of fungal cell walls with fluorescein isothiocyanate-conjugated wheat germ agglutinin (FITC-WGA), and the combined counters作者: 克制 時間: 2025-3-22 16:10 作者: STYX 時間: 2025-3-22 17:59 作者: vasospasm 時間: 2025-3-22 22:49
Isolation and Culture of Arbuscular Mycorrhizal Fungi from Field Samples,gal inoculant in substrate-based manufacturing. In addition, critical parameters are presented for the optimal production of arbuscular mycorrhizal fungal inoculum. All the steps of the process are enlisted: from choosing the source of inoculum, its production, scaling, sustaining quality control, to shelf life.作者: Mettle 時間: 2025-3-23 04:06
High-Throughput DNA Sequence-Based Analysis of AMF Communities, high-throughput sequencing through Illumina MiSeq platform of two DNA target regions: small subunit (SSU) and internal transcribed spacer (ITS). The protocol can apply to both soil and root AMF communities.作者: phytochemicals 時間: 2025-3-23 06:24 作者: GRAVE 時間: 2025-3-23 11:19 作者: 上下連貫 時間: 2025-3-23 16:40 作者: 世俗 時間: 2025-3-23 19:34 作者: 隱士 時間: 2025-3-24 01:41
https://doi.org/10.1007/b106006 in the sample pool is identified for, later, match their presence in the different samples contained in the experiment. A final AM fungal operational taxonomic unit x sample presence–absence matrix is obtained, which allows the subsequent multivariate statistical analysis of the experimental results.作者: 財主 時間: 2025-3-24 03:38
Ernest S. Kahane,William McCookia. In this protocol, we describe the combination of extra-radical mycelium growth systems with gel-based extraction of fungal peptides amenable for shotgun protein profiling, which allows gaining information about the extra-radical proteome.作者: 等級的上升 時間: 2025-3-24 07:55
Evaluating Corporate Financial Performances for the detection of fungal transcripts. This includes chemical fixation, subsequent embedding in a suitable medium, sectioning and pretreatment of sections, the hybridization procedure itself, as well as the immunological detection of RNA-RNA hybrids.作者: 性別 時間: 2025-3-24 12:04
Detection of Arbuscular Mycorrhizal Fungal Gene Expression by In Situ Hybridization,s for the detection of fungal transcripts. This includes chemical fixation, subsequent embedding in a suitable medium, sectioning and pretreatment of sections, the hybridization procedure itself, as well as the immunological detection of RNA-RNA hybrids.作者: savage 時間: 2025-3-24 17:50 作者: Acumen 時間: 2025-3-24 21:56
Guardianship and Conservatorship,quent microscopic visualization, such as the application of enzyme-based stains, NADPH formation, and also nucleus staining. Some modified methods for the extraction of mycelium from the soil are also presented.作者: 黃瓜 時間: 2025-3-24 23:59
Not Guilty by Reason of Insanity,for each experimental system and is therefore covered by general recommendations. Subsequently, specific steps are described for the validation of the assay using a standard dilution series, the determination of appropriate dilutions of DNA extracts from roots, and the quantification of the gene copy numbers in samples including calculations.作者: 音的強弱 時間: 2025-3-25 04:51 作者: albuminuria 時間: 2025-3-25 07:51 作者: 鞭子 時間: 2025-3-25 11:51 作者: CORE 時間: 2025-3-25 17:24
Arbuscular Mycorrhizal Fungal Gene Expression Analysis by Real-Time PCR,ect identification of differentially expressed genes, appropriate controls are required in order to minimize nonspecific variations associated with intrinsic technical variability. In this chapter, we recommend a number of tips to use qRT-PCR analysis in mycorrhizal roots and fungal mycelium.作者: 絕種 時間: 2025-3-25 21:30 作者: Fibrinogen 時間: 2025-3-26 00:51 作者: Concrete 時間: 2025-3-26 05:03
https://doi.org/10.1007/b106006 to particular requirements, such as the type of root material or the reduction of toxic products. In addition, we also summarize some of the most common ways to quantify arbuscular mycorrhizal colonization.作者: 合法 時間: 2025-3-26 11:39
Level Four and Beyond: An Roi Model this purpose. Here we present the yeast methods required for the functional analysis of AM fungal genes, including protocols for yeast transformation, heterologous gene expression, functional complementation assays, preparation of yeast extracts, and subcellular localization of the encoded protein.作者: 線 時間: 2025-3-26 15:31
https://doi.org/10.1007/978-94-011-4850-4ing with plants, including the fungi of the arbuscular mycorrhizal symbiosis. Here, we present a protocol developed in our laboratory to downregulate genes from the obligate symbiont . in symbiosis with . plants.作者: Spinal-Tap 時間: 2025-3-26 19:50
Quality of Life and Longer Term Outcomes of . with these viral RNAs results in reconstitution of the virus in the plant, which triggers silencing of the fungal gene. Here, we describe not only the methods but also several tips for successful application of virus-induced gene silencing to AM fungi.作者: Gratuitous 時間: 2025-3-27 00:55 作者: 史前 時間: 2025-3-27 01:59
Functional Analysis of Arbuscular Mycorrhizal Fungal Genes in Yeast, this purpose. Here we present the yeast methods required for the functional analysis of AM fungal genes, including protocols for yeast transformation, heterologous gene expression, functional complementation assays, preparation of yeast extracts, and subcellular localization of the encoded protein.作者: Antigen 時間: 2025-3-27 07:42 作者: 誘拐 時間: 2025-3-27 12:16
Application of Virus-Induced Gene Silencing to Arbuscular Mycorrhizal Fungi, of . with these viral RNAs results in reconstitution of the virus in the plant, which triggers silencing of the fungal gene. Here, we describe not only the methods but also several tips for successful application of virus-induced gene silencing to AM fungi.作者: CLAM 時間: 2025-3-27 14:22
Isolation and Culture of Arbuscular Mycorrhizal Fungi from Field Samples,t techniques to isolate and produce large-scale arbuscular mycorrhizal fungi-based inoculum, being soil, inert substrate, and in vitro culture techniques among the most used by different biofertilizer producers. This chapter describes an active operating method to isolate and produce large-scale fun作者: 低能兒 時間: 2025-3-27 18:42 作者: 漂白 時間: 2025-3-27 22:55
Histochemical Staining and Quantification of Arbuscular Mycorrhizal Fungal Colonization, Here we describe a standardized method for staining of AMF in colonized roots, and we provide possible modifications to adjust the protocol according to particular requirements, such as the type of root material or the reduction of toxic products. In addition, we also summarize some of the most com作者: Thyroid-Gland 時間: 2025-3-28 05:21
Fluorescent Staining of Arbuscular Mycorrhizal Structures Using Wheat Germ Agglutinin (WGA) and Protercellular and intracellular spaces, leading to the coexistence of diverse intraradical structures, such as hyphae, coils, arbuscules, and vesicles. In addition, the relative abundance of intercellular and intracellular fungal structures is highly dependent on root anatomy and the combination of pl作者: 影響 時間: 2025-3-28 08:56
Visualization of Arbuscular Mycorrhizal Fungal Extraradical Hyphae and Spores Vitality and Activityd water uptake and as propagules that allow the perpetuation of the AM symbiosis over time, respectively. However, the attention of scientific literature is usually more focused on root colonization than on the study of the extraradical components of AM fungi, especially their vital, active, or func作者: 商議 時間: 2025-3-28 13:54 作者: 抗體 時間: 2025-3-28 15:00 作者: 懦夫 時間: 2025-3-28 19:52
High-Throughput DNA Sequence-Based Analysis of AMF Communities,utrition and water supply, soil structure, and plant resistance to pathogens. Investigating AMF presence and distribution at small and large scales is critical. Therefore, research requires standard protocols to be easily implemented. In this chapter, we describe a workflow for AMF identification by作者: instill 時間: 2025-3-28 23:58
Analysis of Arbuscular Mycorrhizal Fungal Communities by Terminal Restriction Fragment Length Polymm root or soil material DNA extraction. The low taxonomic diversity of arbuscular mycorrhizal (AM) fungi makes this technique a cheap and adequate method for fingerprinting their communities. Here, I describe the TRFLP database approach, a version of the technique in which the AM fungal taxa present作者: 朦朧 時間: 2025-3-29 06:31
Bioinformatic Methods for the Analysis of High-Throughput RNA Sequencing in Arbuscular Mycorrhizal is focused on protein-coding RNAs, and results in large datasets of reads, or portion of sequenced mRNA that can be assembled back to the original transcripts to reconstruct a virtual gene catalog. Studies on the biology of arbuscular mycorrhizal fungi (AMF) often took great advantage of mRNA-seq, 作者: Ambiguous 時間: 2025-3-29 07:32 作者: EXPEL 時間: 2025-3-29 11:43
Laser Microdissection as a Useful Tool to Study Gene Expression in Plant and Fungal Partners in AM ions during plant–microbe interactions. In this chapter, protocols to apply the LMD approach to study plant and fungal transcript profiles in different cell-type populations from arbuscular mycorrhizal (AM) roots are described in detail, starting from the biological material preparation to gene expr作者: Keratin 時間: 2025-3-29 16:18
Detection of Arbuscular Mycorrhizal Fungal Gene Expression by In Situ Hybridization,s. In particular, to capture the dynamic of the interaction, cell-specific methods for gene expression analysis are required. In situ hybridization is a technique that allows to determine the location of transcript accumulation within tissues, being of special interest for these fungi that cannot be作者: negotiable 時間: 2025-3-29 22:01
Functional Analysis of Arbuscular Mycorrhizal Fungal Genes in Yeast,ason, a heterologous system has been traditionally used for functional analysis of AM fungal genes, being the budding yeast . an organism suitable for this purpose. Here we present the yeast methods required for the functional analysis of AM fungal genes, including protocols for yeast transformation作者: clarify 時間: 2025-3-30 00:18
Recovery of Extra-Radical Fungal Peptides Amenable for Shotgun Protein Profiling in Arbuscular Mycohe occupation of different niches, and dispersal of propagules, but also has strong influences upon biogeochemical cycling. By providing a valuable overview of expression changes of most proteins, shotgun proteomics can help decipher key metabolic pathways involved in the functioning of fungal mycel作者: cardiopulmonary 時間: 2025-3-30 06:55
Host-Induced Gene Silencing of Arbuscular Mycorrhizal Fungal Genes via ,-Mediated Root Transformatihat are not amenable or recalcitrant to genetic modifications. This method has been particularly used for oomycetes and for filamentous fungi interacting with plants, including the fungi of the arbuscular mycorrhizal symbiosis. Here, we present a protocol developed in our laboratory to downregulate 作者: 珍奇 時間: 2025-3-30 12:10
Application of Virus-Induced Gene Silencing to Arbuscular Mycorrhizal Fungi,ite of CMV2-A1 vector developed from RNA2 of . Y strain, and the RNA2, together with RNA1 and RNA3 of the virus, are in vitro-transcribed. Inoculation of . with these viral RNAs results in reconstitution of the virus in the plant, which triggers silencing of the fungal gene. Here, we describe not on作者: Accrue 時間: 2025-3-30 16:24
Nuria Ferrol,Luisa LanfrancoIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts作者: nuclear-tests 時間: 2025-3-30 17:06
Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/161148.jpg作者: stress-response 時間: 2025-3-30 22:03 作者: ONYM 時間: 2025-3-31 04:24 作者: Presbycusis 時間: 2025-3-31 05:37 作者: CANDY 時間: 2025-3-31 09:41
https://doi.org/10.1007/978-3-319-45225-8lium (ERM) produced by different arbuscular mycorrhizal fungi (AMF). In this system, a host plant germling is inoculated with AMF to establish mycorrhizal symbiosis, and, after colonization, newly formed extraradical hyphae and spores are removed. Then the mycorrhizal root system is wrapped in a nyl作者: HILAR 時間: 2025-3-31 15:08 作者: Lucubrate 時間: 2025-3-31 18:09
Guardianship and Conservatorship,tercellular and intracellular spaces, leading to the coexistence of diverse intraradical structures, such as hyphae, coils, arbuscules, and vesicles. In addition, the relative abundance of intercellular and intracellular fungal structures is highly dependent on root anatomy and the combination of pl作者: dendrites 時間: 2025-3-31 22:05 作者: Hot-Flash 時間: 2025-4-1 02:10 作者: 群居男女 時間: 2025-4-1 08:48 作者: 包庇 時間: 2025-4-1 11:45
Not Guilty by Reason of Insanity,utrition and water supply, soil structure, and plant resistance to pathogens. Investigating AMF presence and distribution at small and large scales is critical. Therefore, research requires standard protocols to be easily implemented. In this chapter, we describe a workflow for AMF identification by作者: 漂浮 時間: 2025-4-1 15:42 作者: 哥哥噴涌而出 時間: 2025-4-1 22:08
Competence to Consent to Treatment, is focused on protein-coding RNAs, and results in large datasets of reads, or portion of sequenced mRNA that can be assembled back to the original transcripts to reconstruct a virtual gene catalog. Studies on the biology of arbuscular mycorrhizal fungi (AMF) often took great advantage of mRNA-seq,
