作者: 沖突 時間: 2025-3-21 22:02 作者: 知識 時間: 2025-3-22 03:33
Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/160592.jpg作者: esthetician 時間: 2025-3-22 06:57
Erziehungsziel "Selbstst?ndigkeit"ia that can be employed include soil, commercial greenhouse mixes, vermrcuhte, and other relatively inert media watered wtth nutrient solutions, as well as agar. This chapter focuses on growth of plants on so11 in various envrromnental settings and especrally m growth chambers and greenhouses. Harve作者: 不可知論 時間: 2025-3-22 12:43 作者: –scent 時間: 2025-3-22 15:03
Erziehungsziel "Selbstst?ndigkeit"are mamtamed for mapping analysis, and potentially useful new mutants are not lost m the early fragile stages of screening. . 1s prone to many of the pests that commonly infect greenhouses and growthrooms (.,.). These can cause severe damage to the plants and, m some cases, can kill them. In general作者: 取之不竭 時間: 2025-3-22 17:03
Konzepte konstruktivistischen Denkensed (.). Cell suspensions are used for generating large amounts of cells for quantitative or qualitative analysis of growth responses and metabolism of novel chemicals, as well as for studies of cell cycle under standard conditions (.,.). In addition, cell suspensions serve as an ideal material for t作者: 抑制 時間: 2025-3-22 22:06
Erziehungsziel "Selbstst?ndigkeit"Alternatively, plants can be regenerated from unorganized callus tissues derived from different explants by dedifferentiation induced by exogenous growth regulators. Plant regeneration from call1 is possible by . organogenesis or somatic embryogenesis. Callus cultures also facilitate the amphficatio作者: Arb853 時間: 2025-3-23 03:45
Erziehungsziel "Selbstst?ndigkeit" problems in achieving a high frequency of sustainable division of protoplasts in liquid culture. The problem can be alleviated to a certain extent by embedding mesophyll protoplasts m calcium algmate (.). We have developed a technique allowing the culture of protoplasts derived from roots or cell s作者: Malaise 時間: 2025-3-23 08:54 作者: 光明正大 時間: 2025-3-23 10:18 作者: INTER 時間: 2025-3-23 17:31
Problemstellung und Vorgehensweise isolation are often designed to produce large amounts of DNA of high molecular weight with sufficient purity. Since the polymerase chain reaction (PCR) 1s the method of choice in modern plant genetic analyses such as map-based breeding and positional gene clonmg, many recently developed protocols a作者: 氣候 時間: 2025-3-23 19:24
Erziehungsziel "Selbstst?ndigkeit"iving tissues generally do not allow recovery of DNA fragments larger than 50‐100 kb m size. In recent years, technical improvements have made possible to purify and analyze large DNA molecules in vitro. Techniques such as pulsed‐field agarose gel electrophoresis (PFGE) (.) or cloning m yeast artifi作者: yohimbine 時間: 2025-3-24 00:19 作者: grovel 時間: 2025-3-24 02:44
Problemstellung und Vorgehensweise DNA (mtDNA). A prerequisite to purify mtDNA with little contamination of nuclear and also of plastid DNA is the isolatton of mitochondrta from cells. Mitochondria from green plants are generally dtfticult to purify because of the low amount of these organelles and the high number of chloroplasts m 作者: 樂器演奏者 時間: 2025-3-24 06:38
Konzepte konstruktivistischen Denkensure that bridges the interface between the experimental manipulation of the hvmg system, and the subsequent analysis of effects through molecular techniques. . is an ideal system for such analyses. Large quantities of plant material are easily obtained, from which mrlligram quantities of total RNA m作者: adduction 時間: 2025-3-24 13:05 作者: 膠狀 時間: 2025-3-24 15:56
Ausgangslage und theoretische Grundlagen,andbook of genetics. It is only owing to the specific (reproductive) biology of a species that sometimes a certain analytical procedure is favored over another one For . the fact that it is a self-fertilizing species and that it has relatively small flowers are important in this respect.作者: DECRY 時間: 2025-3-24 20:34 作者: 想象 時間: 2025-3-24 23:52
Arabidopsis Protocols978-1-59259-268-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Exclude 時間: 2025-3-25 04:31 作者: 出沒 時間: 2025-3-25 09:37
Ausgangslage und theoretische Grundlagen,andbook of genetics. It is only owing to the specific (reproductive) biology of a species that sometimes a certain analytical procedure is favored over another one For . the fact that it is a self-fertilizing species and that it has relatively small flowers are important in this respect.作者: CANT 時間: 2025-3-25 12:01
Book 19981st editionin every area of plant biology, have served to mobilize resources and orient much new research towards the plant field, where the special biological features of Arabidopsis have further focused attention on this useful species. The high level of interest in Arabidopsis has worked as a catalyst and, 作者: scrutiny 時間: 2025-3-25 17:43
Sterile Techniques in Arabidopsisnd catabolism of the compound by other organisms. Aside from the ease of screening, Petri plate screens also have the advantage that retesting and genetic analysts of subsequent generations can be done under exactly the same condmons as the original screen.作者: AIL 時間: 2025-3-25 23:18 作者: 細(xì)查 時間: 2025-3-26 01:19 作者: Cleave 時間: 2025-3-26 05:18
Chloroplast DNA Isolationhloroplast DNA because intact chloroplasts have a nonporous envelope. In the second method, the intact chloroplasts are banded in a sucrose— or a percoll—density—gradient, while the nuclei pellet. Banded chloroplasts are carefully removed. Intact chloroplasts, obtained by either a DNase method or a 作者: Badger 時間: 2025-3-26 12:05
12 Purification of Mitochondrial DNA from Green Tissues of Arabidopsisltures are available. Furthermore, tissue cultures are not always the ideal source for mvestigations of mitochondrial genome structure, since frequent reorganizattons have been reported for such tissue cultures, which distort the inplanta structure of these genomes (.,.).作者: sclera 時間: 2025-3-26 14:57
Preparation of RNAalso spawned variations, most notably a single‐step method that ehmmates an ultracentrifugation step (3). Although this method may compare favorably with the procedures presented herein with regard to technical simplicity, our protocol consistently results m high yields of intact RNA of a quality su作者: 吹牛需要藝術(shù) 時間: 2025-3-26 17:24 作者: BRACE 時間: 2025-3-26 23:22
Erziehungsziel "Selbstst?ndigkeit"nd catabolism of the compound by other organisms. Aside from the ease of screening, Petri plate screens also have the advantage that retesting and genetic analysts of subsequent generations can be done under exactly the same condmons as the original screen.作者: 棲息地 時間: 2025-3-27 02:41
Konzepte konstruktivistischen Denkensopszs yield well proliferating cell suspensions capable of morphogenesis m liquid medium. The regeneration capability and genetic stability of suspension cells, however, decrease by the length of culture period Therefore, it is recommended to use newly initiated cell suspensions when the application作者: Systemic 時間: 2025-3-27 09:03
Erziehungsziel "Selbstst?ndigkeit"atron, can also be obtained from cell suspension cultures, excluding any limitatton of available starting material. Because the methods may have to be tailored to the needs of different experiments, here we describe protocols for the rsolatlon and culture of protoplasts from leaf mesophyll tissue (.作者: Breach 時間: 2025-3-27 11:38
Erziehungsziel "Selbstst?ndigkeit"hloroplast DNA because intact chloroplasts have a nonporous envelope. In the second method, the intact chloroplasts are banded in a sucrose— or a percoll—density—gradient, while the nuclei pellet. Banded chloroplasts are carefully removed. Intact chloroplasts, obtained by either a DNase method or a 作者: 炸壞 時間: 2025-3-27 15:27
Problemstellung und Vorgehensweiseltures are available. Furthermore, tissue cultures are not always the ideal source for mvestigations of mitochondrial genome structure, since frequent reorganizattons have been reported for such tissue cultures, which distort the inplanta structure of these genomes (.,.).作者: ABHOR 時間: 2025-3-27 20:48
Konzepte konstruktivistischen Denkensalso spawned variations, most notably a single‐step method that ehmmates an ultracentrifugation step (3). Although this method may compare favorably with the procedures presented herein with regard to technical simplicity, our protocol consistently results m high yields of intact RNA of a quality su作者: 開花期女 時間: 2025-3-28 00:06
https://doi.org/10.1007/978-3-8349-8472-2 mdividuals actually treated with the mutagen are referred to as the M. generation. The progeny derived from the self-fertilization of these individuals are referred to as the M. generation. The M. generation is the first generation following mutagenesis in which homozygous recessive mutations can b作者: 秘方藥 時間: 2025-3-28 05:10
1064-3745 ological features of Arabidopsis have further focused attention on this useful species. The high level of interest in Arabidopsis has worked as a catalyst and, 978-1-59259-268-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: CLAN 時間: 2025-3-28 07:29 作者: 合唱隊 時間: 2025-3-28 14:24 作者: 表示向前 時間: 2025-3-28 15:21 作者: 最后一個 時間: 2025-3-28 18:52 作者: Postulate 時間: 2025-3-28 23:39
Callus Culture and RegenerationAlternatively, plants can be regenerated from unorganized callus tissues derived from different explants by dedifferentiation induced by exogenous growth regulators. Plant regeneration from call1 is possible by . organogenesis or somatic embryogenesis. Callus cultures also facilitate the amphficatio作者: 誘使 時間: 2025-3-29 04:51 作者: Schlemms-Canal 時間: 2025-3-29 07:14 作者: Goblet-Cells 時間: 2025-3-29 11:31
Purification of Mitochondria from Arabidopsisdes most of the nonphotosynthetrc energy required m the cell. Mitochondria contain their own genome(s), but encode only a small number of vita1 gene products. Simrlar plastids, the majority of the organellar proteins are nuclear encoded, and imported into these organelles.作者: patella 時間: 2025-3-29 17:18
Preparation of DNA from , isolation are often designed to produce large amounts of DNA of high molecular weight with sufficient purity. Since the polymerase chain reaction (PCR) 1s the method of choice in modern plant genetic analyses such as map-based breeding and positional gene clonmg, many recently developed protocols a作者: jettison 時間: 2025-3-29 22:31 作者: micronized 時間: 2025-3-30 01:24
Chloroplast DNA Isolationatory procedure for isolating chloroplast DNA from . with minimal nuclear or mitochondrial DNA contamination. In general, a first step in isolating chloroplast DNA is the homogenization of the plant material followed by a filtration step to remove large-sized cell debris and cell fragments. The filt作者: Rustproof 時間: 2025-3-30 05:43
12 Purification of Mitochondrial DNA from Green Tissues of Arabidopsis DNA (mtDNA). A prerequisite to purify mtDNA with little contamination of nuclear and also of plastid DNA is the isolatton of mitochondrta from cells. Mitochondria from green plants are generally dtfticult to purify because of the low amount of these organelles and the high number of chloroplasts m 作者: MERIT 時間: 2025-3-30 08:41 作者: 剛毅 時間: 2025-3-30 12:50 作者: 不滿分子 時間: 2025-3-30 19:11 作者: aqueduct 時間: 2025-3-30 23:43 作者: Itinerant 時間: 2025-3-31 04:06
Book 19981st editionies, its short generation time, and the thousands of seeds produced by each plant attracted the interest of geneticists. In the middle of the century, this plant had already gained a place in several genetics laboratories both in Europe and the United States, being widely used in mutagenesis experim作者: DEAWL 時間: 2025-3-31 08:23
Karl Nikolaus Renner,Rebecca Wessinghagewever, despite the small size of chromosomes, the rapid advances in chromosome preparation methods, fluorescent microscopy, and staining since 1990 mean that analysis IS now straightforward (see ...–.).作者: Mitigate 時間: 2025-3-31 12:28 作者: 榮幸 時間: 2025-3-31 17:03 作者: Deadpan 時間: 2025-3-31 19:51 作者: Gorilla 時間: 2025-3-31 22:45
Preparation of Physiologically Active Chloroplasts from Arabidopsismatic machinery involved m fatty acid biosynthesis and fatty acid incorporation into chloroplast lipids In some plant species, like spinach or pea, highly active chloroplasts can be obtained by followmg several simple rules:作者: Gourmet 時間: 2025-4-1 02:00 作者: 揮舞 時間: 2025-4-1 06:01
Erziehungsziel "Selbstst?ndigkeit"physical mapping of complex genomes like Arabidopsis (.,.). Physical mapping of eukaryotic genomes generally involves genome reconstruction using ordered clones from large‐insert genomic libraries m YACs. Several Arabidopsis YAC libraries are available (.-.).作者: 受傷 時間: 2025-4-1 12:53 作者: Concerto 時間: 2025-4-1 15:33 作者: 跳脫衣舞的人 時間: 2025-4-1 19:39 作者: 哭得清醒了 時間: 2025-4-2 00:09 作者: OVER 時間: 2025-4-2 05:52 作者: 品嘗你的人 時間: 2025-4-2 09:50
Growth of Plants and Preservation of Seedsll as agar. This chapter focuses on growth of plants on so11 in various envrromnental settings and especrally m growth chambers and greenhouses. Harvestmg, seed quaky, and seed preservation are also considered.
