作者: 震驚 時(shí)間: 2025-3-21 23:59
Sozialp?dagogik — Handeln in Widersprüchen?derstand the natural variation for any trait and gene: (1) the phenotypic analysis of Arabidopsis plants grown in field experiments; (2) the analysis of nucleotide diversity and environmental associations for specific genes.作者: 預(yù)知 時(shí)間: 2025-3-22 02:25
Qualifikationsprofil: Diplom-P?dagogInere we describe a non-sterile grafting method involving an insect pin to secure the scion to the rootstock. The scions can be grafted onto epicotyls or hypocotyls of soil-grown Arabidopsis rootstocks at a wide range of developmental stages. This grafting method provides a useful tool to analyze leaf-derived mobile signals in Arabidopsis.作者: Maximize 時(shí)間: 2025-3-22 08:04
Heinz-Hermann Krüger,Winfried Marotzkiassay allows high-throughput screening of new constructs and the study of the localization of a large number of subcellular markers in . seedlings including wild-type, stable over-expressing and mutant lines.作者: 跑過(guò) 時(shí)間: 2025-3-22 11:32 作者: corpus-callosum 時(shí)間: 2025-3-22 15:20 作者: crockery 時(shí)間: 2025-3-22 21:00 作者: 連鎖,連串 時(shí)間: 2025-3-22 21:32 作者: Feigned 時(shí)間: 2025-3-23 04:38 作者: Incorruptible 時(shí)間: 2025-3-23 05:42
Non-sterile Grafting Methods for Arabidopsisere we describe a non-sterile grafting method involving an insect pin to secure the scion to the rootstock. The scions can be grafted onto epicotyls or hypocotyls of soil-grown Arabidopsis rootstocks at a wide range of developmental stages. This grafting method provides a useful tool to analyze leaf-derived mobile signals in Arabidopsis.作者: Countermand 時(shí)間: 2025-3-23 10:27 作者: 太空 時(shí)間: 2025-3-23 17:57
Arabidopsis?Photosynthetic and Heterotrophic Cell Suspension Culturesvitro systems may allow to obtain insights into the unique features of chloroplasts versus non-green plastids, as well as their integration in the structural and metabolic compartmentalization of the plant cell.作者: kindred 時(shí)間: 2025-3-23 19:40
Visualizing and Measuring Single Locus Dynamics in r nuclear processes. Approaches such as gene tagging have been established for living cells in order to detect, track, and analyze the mobility of single loci. In this chapter, we provide an experimental protocol for performing locus tracking in . roots and for characterizing locus mobility behavior via a Mean Square Displacement analysis.作者: Diluge 時(shí)間: 2025-3-24 01:19
Live Imaging of Arabidopsis Leaf and Vegetative Meristem Developmentopsis inflorescence meristem, gaining access to the vegetative meristem for imaging has been considered more difficult. Here we describe a method to dissect young Arabidopsis seedlings in order to obtain a clear view of the vegetative meristem and young leaf primordia using confocal microscopy.作者: Myocyte 時(shí)間: 2025-3-24 06:13 作者: 擔(dān)心 時(shí)間: 2025-3-24 07:45
Book 2021Latest edition, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls...Authoritative and cutting-edge, .Arabidopsis Protocols, Fourth Edition .aims to ensure successful results in the further study of this vital field..作者: 顯赫的人 時(shí)間: 2025-3-24 13:09 作者: Anticoagulant 時(shí)間: 2025-3-24 18:31
Heinz-Hermann Krüger,Winfried Marotzkiassay for protein trafficking and localization study. Further, using GFP-tagged ERES (Endoplasmic Reticulum Exit Site) marker proteins and RFP-tagged Golgi marker as examples, we illustrate the major tools and methods for protein localization analysis using super-resolution imaging.作者: 評(píng)論性 時(shí)間: 2025-3-24 22:21 作者: 鄙視 時(shí)間: 2025-3-25 02:18 作者: 云狀 時(shí)間: 2025-3-25 04:56
Transient Expression of Fluorescent Fusion Proteins in , Protoplastsassay for protein trafficking and localization study. Further, using GFP-tagged ERES (Endoplasmic Reticulum Exit Site) marker proteins and RFP-tagged Golgi marker as examples, we illustrate the major tools and methods for protein localization analysis using super-resolution imaging.作者: Deject 時(shí)間: 2025-3-25 08:49 作者: 語(yǔ)言學(xué) 時(shí)間: 2025-3-25 13:30
Using Tripartite Split-sfGFP for the Study of Membrane Protein–Protein Interactionshighly expressed and accessible to the compartments of interaction. Here we describe a protocol for using the ?-Estradiol-inducible tripartite split-sfGFP assay for side-by-side analyses of in vivo PPI along with in situ subcellular localization of fusion proteins in agroinfiltrated . leaves.作者: 使服水土 時(shí)間: 2025-3-25 15:55
Barbara Friebertsh?user,Margret Kraulfication enrichment for sets of genes, and exploring protein–protein and protein–DNA interactions in Arabidopsis. We will also cover additional tools that allow integration of data from several sources for improved hypothesis generation.作者: 清晰 時(shí)間: 2025-3-26 00:01 作者: hieroglyphic 時(shí)間: 2025-3-26 00:30
Bioinformatic Tools in , Researchfication enrichment for sets of genes, and exploring protein–protein and protein–DNA interactions in Arabidopsis. We will also cover additional tools that allow integration of data from several sources for improved hypothesis generation.作者: 使殘廢 時(shí)間: 2025-3-26 07:00 作者: 絕緣 時(shí)間: 2025-3-26 11:59 作者: 散開 時(shí)間: 2025-3-26 12:57 作者: 拋物線 時(shí)間: 2025-3-26 19:15 作者: 不發(fā)音 時(shí)間: 2025-3-27 00:11 作者: 叢林 時(shí)間: 2025-3-27 01:46
1064-3745 ..Authoritative and cutting-edge, .Arabidopsis Protocols, Fourth Edition .aims to ensure successful results in the further study of this vital field..978-1-0716-0882-1978-1-0716-0880-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: DENT 時(shí)間: 2025-3-27 05:54
Hans-Uwe Otto,Thomas Rauschenbachlarge amount of plant material. The importance of controlling, observing, and recording growth conditions is emphasized and appropriate equipment for monitoring these conditions is listed. Proper conditions for seed harvest and preservation, as well as seed quality control procedures, are also descr作者: intrude 時(shí)間: 2025-3-27 09:38
P?dagogische Arbeit in Organisationenem. Specifically, the steps of target gene selection, sgRNA design, vector construction, transformation, and analysis of transgenic lines are described. The protocol is potentially adaptable to perform genome editing in other plant species such as rice.作者: 珍奇 時(shí)間: 2025-3-27 17:06
https://doi.org/10.1007/978-3-658-05203-4ration with other types of information to overcome the limited statistical power of GWAS. This chapter describes the stepwise procedure of GWAS in . focusing on data analysis processes including preprocessing of genotype and phenotype data, statistical analysis to identify phenotype-associated chrom作者: Bronchial-Tubes 時(shí)間: 2025-3-27 19:54
https://doi.org/10.1007/978-3-531-18738-9which updates work last reviewed in 2014 [Galbraith DW (2014) Flow cytometry and sorting in Arabidopsis. In: Sanchez Serrano JJ, Salinas J (eds) Arabidopsis Protocols, 3rd ed. Methods in molecular biology, vol 1062. Humana Press, Totowa, pp 509–537]. describes the application of techniques of flow c作者: CANDY 時(shí)間: 2025-3-28 01:43
Arabidopsis Protocols978-1-0716-0880-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 偽造 時(shí)間: 2025-3-28 02:06
https://doi.org/10.1007/978-1-0716-0880-7Arabidopsis related model species; Bioinformatics; CRISPR-Cas; RNA sequencing; transcriptomics作者: 行乞 時(shí)間: 2025-3-28 09:32 作者: cornucopia 時(shí)間: 2025-3-28 11:59
Hans-Uwe Otto,Thomas Rauschenbachce Center (ABRC) has collected and developed a series of best-practice protocols, some of which are presented in this chapter. Arabidopsis can be grown in a variety of locations, growth media, and environmental conditions. Some mutant genotypes, natural accessions, and Arabidopsis relatives require 作者: 失望未來(lái) 時(shí)間: 2025-3-28 15:34 作者: 跟隨 時(shí)間: 2025-3-28 22:13
Sozialp?dagogik — Handeln in Widersprüchen?mong and within natural populations. Specially, the recent availability of large worldwide collections of accessions, together with their full genome sequences, has triggered the study of Arabidopsis natural variation. In this chapter, we describe two protocols that exploit these new resources to un作者: 諷刺 時(shí)間: 2025-3-29 02:01
Qualifikationsprofil: Diplom-P?dagogInnt of the mobile signals. The mutant collection of Arabidopsis offers background-free living materials for examining the transport of mobile signals .. In the past few years, many grafting methods have been developed to overcome the limitations of rosette-type growth and small size in Arabidopsis. H作者: SEEK 時(shí)間: 2025-3-29 04:05 作者: photopsia 時(shí)間: 2025-3-29 10:27
Heinz-Hermann Krüger,Winfried Marotzkiansient transformation assay with five-day-old seedlings using .-mediated vacuum infiltration. Three days after infiltration of the . containing an expression vector for a fluorescent marker of interest, cotyledon cells expressing the fluorescent protein can be imaged in a confocal microscope. This 作者: aquatic 時(shí)間: 2025-3-29 12:19
Heinz-Hermann Krüger,Winfried Marotzkion and dynamics in plant cells. Recently, super-resolution imaging techniques such as N-SIM (Nikon, Structured Illumination Microscopy) are widely used in cell biology study, allowing cell biologists to obtain unattainable details and relationships of cell structures and functions by conventional co作者: groggy 時(shí)間: 2025-3-29 19:15 作者: 擴(kuò)張 時(shí)間: 2025-3-29 22:50
https://doi.org/10.1007/978-3-658-05203-4animals, and plants. Since most phenotypes of plant species are complex traits regulated by many genes and their functional interactions, GWAS are increasing in popularity for genetic dissections of plant phenotypes. For the reference plant, ., detailed information on genetic variations became avail作者: 水槽 時(shí)間: 2025-3-30 00:28
https://doi.org/10.1007/978-3-658-05203-4now clear that chromatin proteins and the chromatin fiber itself are in fact very dynamic. For instance, the packaging of the DNA into the nucleus requires an extraordinary degree of compaction but this should be achieved without compromising the accessibility to the transcription machinery and othe作者: BAIL 時(shí)間: 2025-3-30 04:21 作者: COUCH 時(shí)間: 2025-3-30 10:15 作者: mendacity 時(shí)間: 2025-3-30 14:11 作者: 吼叫 時(shí)間: 2025-3-30 19:07
Susann Fegter,Fabian Kessl,Daniel Wranatemporal dynamics of cellular regulators. Fluorescent protein (FP)-based biosensors are extensively employed due to their high selectivity and low invasiveness. A variety of FP-based biosensors have been engineered and applied in plant research to visualize dynamic changes in pH, redox state, concen作者: 半球 時(shí)間: 2025-3-31 00:13 作者: Exposure 時(shí)間: 2025-3-31 03:45
Cathleen Grunert,Karin Bock,Wolfgang Schr?er/freeze-substitution methods with other imaging techniques such as correlative light and electron microscopy (CLEM), electron tomography (ET), and immunogold-labeling provides unique opportunities to understand structural changes associated with cellular processes. This chapter presents the main ste作者: Innocence 時(shí)間: 2025-3-31 07:15
Jose J. Sanchez-Serrano,Julio SalinasIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: Ligament 時(shí)間: 2025-3-31 13:07 作者: 捐助 時(shí)間: 2025-3-31 16:40
