標(biāo)題: Titlebook: Applications of Genome Modulation and Editing; Paul John Verma,Huseyin Sumer,Jun Liu Book 2022 The Editor(s) (if applicable) and The Autho [打印本頁(yè)] 作者: 畸齒矯正學(xué) 時(shí)間: 2025-3-21 16:42
書(shū)目名稱(chēng)Applications of Genome Modulation and Editing影響因子(影響力)
書(shū)目名稱(chēng)Applications of Genome Modulation and Editing影響因子(影響力)學(xué)科排名
書(shū)目名稱(chēng)Applications of Genome Modulation and Editing網(wǎng)絡(luò)公開(kāi)度
書(shū)目名稱(chēng)Applications of Genome Modulation and Editing網(wǎng)絡(luò)公開(kāi)度學(xué)科排名
書(shū)目名稱(chēng)Applications of Genome Modulation and Editing被引頻次
書(shū)目名稱(chēng)Applications of Genome Modulation and Editing被引頻次學(xué)科排名
書(shū)目名稱(chēng)Applications of Genome Modulation and Editing年度引用
書(shū)目名稱(chēng)Applications of Genome Modulation and Editing年度引用學(xué)科排名
書(shū)目名稱(chēng)Applications of Genome Modulation and Editing讀者反饋
書(shū)目名稱(chēng)Applications of Genome Modulation and Editing讀者反饋學(xué)科排名
作者: ASSAY 時(shí)間: 2025-3-21 21:22
978-1-0716-2303-9The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines作者: RACE 時(shí)間: 2025-3-22 02:48 作者: forager 時(shí)間: 2025-3-22 05:15 作者: 發(fā)生 時(shí)間: 2025-3-22 08:56 作者: 繞著哥哥問(wèn) 時(shí)間: 2025-3-22 14:36
Gérard Duhaime,Sébastien Lévesqueected into mouse embryos at different preimplantation stages. Subsequently, simple DNA plasmids were used to microinject into the pronuclei of fertilized mouse oocytes and that method became the reference for many years. The isolation of embryonic stem cells together with advances in genetics allowe作者: 乏味 時(shí)間: 2025-3-22 19:38 作者: judicial 時(shí)間: 2025-3-23 00:25 作者: TOXIN 時(shí)間: 2025-3-23 04:04
Gérard Duhaime,Sébastien Lévesquection expendable, the available BAC libraries are a precious source for life science. Their comprising of extended genomic regions provides an ideal basis for creating a large targeting vector. Here, we describe the identification of suitable BACs from their libraries and their verification prior to作者: exceptional 時(shí)間: 2025-3-23 06:17
https://doi.org/10.1007/978-3-031-17299-1 the biotechnology industry. In this chapter, we describe an easy to establish, robust, and practical pipeline that can be used to generate immortalized cell lines, from different tissues, to capture cell linage context and validate the tools required for genome editing and genetic modification. Thi作者: 不確定 時(shí)間: 2025-3-23 13:32 作者: Ointment 時(shí)間: 2025-3-23 16:24
Aboriginal Community Well-Being Indexotherwise be addressed in-vitro. Microinjection of zygotes remains the most common technique to generate GM animals to date. Here, we describe the targeted insertion (knock-in) of transgenes by microinjection of 1-cell or 2-cell stage embryos into the murine Rosa26 safe harbor.作者: 善于 時(shí)間: 2025-3-23 20:15
Absolute Subjective Well-Being (ASWB)d in bovine embryos. This technology allows for the precise and specific activation of single endogenous gene expression and also multiplexed gene expression in a simple fashion. Therefore, CRISPR-on has unique advantages over other activator systems and a wide adaptability for studies in basic and 作者: 減至最低 時(shí)間: 2025-3-24 01:42
Aboriginal Peoples Survey, Canada (APS)ch as tephritid fruit flies. As SIT is highly favored due to it being species-specific and environmentally friendly, there are constant efforts to improve the efficiency and efficacy of this method in particular at low pest densities; one of which is the use of genetically enhanced strains. Developm作者: 軍械庫(kù) 時(shí)間: 2025-3-24 06:00
Absolute Subjective Well-Being (ASWB)volution of parasitism. It is because of the latter that some blowflies such as the New World screwworm and the Australian sheep blowfly are considered major economic pests of livestock. Due to their importance, annotated assembled genomes for several species are now available. Here, we present a de作者: 鳴叫 時(shí)間: 2025-3-24 09:46 作者: 現(xiàn)實(shí) 時(shí)間: 2025-3-24 13:50
Absolute Individual Deprivation in Europe/large-sized animals like sheep have several advantages to study human diseases and medicine. Here, we present a protocol that describes the generation of an otoferlin edited sheep model via CRISPR-assisted single-stranded oligodinucleotide-mediated Homology-Directed Repair (HDR), through direct cyt作者: exophthalmos 時(shí)間: 2025-3-24 16:59
Absolute Subjective Well-Being (ASWB)sease of interest and provide a platform for potential therapeutic testing. Pigs are a better model for human disease than rodents because of their genetic and physiological similarities to humans. However, current methods to generate porcine models are both technically challenging and expensive. Ge作者: 闡明 時(shí)間: 2025-3-24 20:23
Absolute Individual Deprivation in Europe Physiol Biochem 121(5):194–205, 2015; Habiela et al. J Gen Virol 95 (Pt 11):2329–2345, 2014) have been proven to be a useful tool for understanding the mechanism behind the phenotypes and fundamental and applied research in livestock. A single base pair deletion of prolactin receptor (.) has an imp作者: 迅速飛過(guò) 時(shí)間: 2025-3-25 01:58 作者: 容易懂得 時(shí)間: 2025-3-25 05:29
Aboriginal Community Well-Being Indexotherwise be addressed in-vitro. Microinjection of zygotes remains the most common technique to generate GM animals to date. Here, we describe the targeted insertion (knock-in) of transgenes by microinjection of 1-cell or 2-cell stage embryos into the murine Rosa26 safe harbor.作者: 完全 時(shí)間: 2025-3-25 07:33
Book 2022ction outcomes. Chapters guide readers through gene regulation, editing, screening of cell lines, genome editing, and an overview of the tools for efficient genome editing including; ZFNs, TALENs, and CRISPR. Written in the format of the highly successful .Methods in Molecular Biology .series, each 作者: ANN 時(shí)間: 2025-3-25 12:33 作者: 盟軍 時(shí)間: 2025-3-25 18:44
Gérard Duhaime,Sébastien Lévesqueasis for creating a large targeting vector. Here, we describe the identification of suitable BACs from their libraries and their verification prior to manipulation. Further, protocols for modifying BAC, confirming the desired modification and the preparation of transfection into mammalian cells are given.作者: dilute 時(shí)間: 2025-3-25 22:03
https://doi.org/10.1007/978-3-031-17299-1ed cell lines, from different tissues, to capture cell linage context and validate the tools required for genome editing and genetic modification. This pipeline serves as a reference for similar approaches for gene interrogation in other species.作者: Heart-Rate 時(shí)間: 2025-3-26 00:16
Aboriginal and Torres Strait Islanderenotypic effects in host cells. Here, we describe the strategy for targeting the AAVS1 site with an inducible Neurogenin-2 (Ngn2) donor template by CRISPR/Cas9 in hiPSCs, which facilitates generation of an inducible cell line that can rapidly and homogenously differentiate into excitatory neurons.作者: infinite 時(shí)間: 2025-3-26 06:20 作者: 評(píng)論性 時(shí)間: 2025-3-26 08:35
Immortalised Cas9-expressing Cell lines for Gene interrogationed cell lines, from different tissues, to capture cell linage context and validate the tools required for genome editing and genetic modification. This pipeline serves as a reference for similar approaches for gene interrogation in other species.作者: Coordinate 時(shí)間: 2025-3-26 14:18
Targeting the AAVS1 Site by CRISPR/Cas9 with an Inducible Transgene Cassette for the Neuronal Differenotypic effects in host cells. Here, we describe the strategy for targeting the AAVS1 site with an inducible Neurogenin-2 (Ngn2) donor template by CRISPR/Cas9 in hiPSCs, which facilitates generation of an inducible cell line that can rapidly and homogenously differentiate into excitatory neurons.作者: Blatant 時(shí)間: 2025-3-26 17:11
https://doi.org/10.1007/978-3-031-17299-1ngle isothermal reaction. We demonstrate an application of this approach to generate a stably transfected pluripotent stem cell line that can be induced to express a transcription factor transgene and rapidly differentiate into neurons in a single step.作者: STALE 時(shí)間: 2025-3-26 21:15
Aboriginal Peoples Survey, Canada (APS)nomic modification of non-model organisms. Here, we describe the manual microinjection of CRISPR/Cas9 reagents into tephritid pest . (Queensland fruit fly) embryos to introduce ideal traits as well as the molecular methods used to detect successful mutagenesis.作者: frugal 時(shí)間: 2025-3-27 03:14
Efficient Generation of Stable Cell Lines with Inducible Neuronal Transgene Expression Using the pigngle isothermal reaction. We demonstrate an application of this approach to generate a stably transfected pluripotent stem cell line that can be induced to express a transcription factor transgene and rapidly differentiate into neurons in a single step.作者: GONG 時(shí)間: 2025-3-27 05:19
CRISPR/Cas9 Mutagenesis to Generate Novel Traits in , for Sterile Insect Techniquenomic modification of non-model organisms. Here, we describe the manual microinjection of CRISPR/Cas9 reagents into tephritid pest . (Queensland fruit fly) embryos to introduce ideal traits as well as the molecular methods used to detect successful mutagenesis.作者: 發(fā)微光 時(shí)間: 2025-3-27 10:50
CRISPR-on for Endogenous Activation of , Expression in Bovine Embryosapplied science, such as cell reprogramming and cell fate differentiation for regenerative medicine..In this chapter, we describe the materials and methods of the CRISPR-on system for activation of the endogenous SMARCA4 expression in bovine embryos作者: 闖入 時(shí)間: 2025-3-27 16:26
CRISPR/Cas9 Genome Editing in the New World Screwworm and Australian Sheep Blowflytailed guide for using the . Cas9 RNA-guided nuclease to efficiently generate both knockout and knock-in mutations in screwworm and sheep blowfly. These methods should accelerate genetic investigations in these and other closely related species and lead to a better understanding of the roles of selected genes in blowfly development and behavior.作者: 自負(fù)的人 時(shí)間: 2025-3-27 18:05
Targeted Gene Editing in Porcine Germ Cellsrmline genetic modification through gene edited spermatogonia provides an effective alternative to how these models are developed. Here, we report an improved technique of gene editing in spermatogonia of pigs using CRISPR-Cas9 to generate different edits that reflect the genotypes of human diseases.作者: Ingenuity 時(shí)間: 2025-3-28 00:15
Book 2022by-step, readily reproducible protocols. ..Authoritative and cutting-edge, .Applications of Genome Modulation and Editing .aims to be a useful and practical guide for researchers to commence or advance their study in this field. .作者: Bouquet 時(shí)間: 2025-3-28 04:12 作者: 畸形 時(shí)間: 2025-3-28 08:19 作者: 經(jīng)典 時(shí)間: 2025-3-28 11:20
Absolute Individual Deprivation in Europeair (HDR) in mouse zygotes. The mouse model carrying the identical . mutation in slick cattle may provide a useful animal model to study the pathway of thermoregulation and the mechanism of heat-tolerance in the livestock.作者: 寬度 時(shí)間: 2025-3-28 15:03 作者: 寬容 時(shí)間: 2025-3-28 21:21
Generation of a Human Deafness Sheep Model Using the CRISPR/Cas System first time an . KI model in sheep with 17.8% edited lambs showing indel mutations, and 61.5% of them bearing knock-in mutations by HDR. The reported method establishes the bases to produce a deafness model to test novel therapies in human disorders related to . mutations.作者: thrombosis 時(shí)間: 2025-3-29 01:44 作者: 暴行 時(shí)間: 2025-3-29 04:28 作者: Urgency 時(shí)間: 2025-3-29 09:29
Absolute Subjective Well-Being (ASWB)tailed guide for using the . Cas9 RNA-guided nuclease to efficiently generate both knockout and knock-in mutations in screwworm and sheep blowfly. These methods should accelerate genetic investigations in these and other closely related species and lead to a better understanding of the roles of selected genes in blowfly development and behavior.作者: 易彎曲 時(shí)間: 2025-3-29 11:53 作者: inconceivable 時(shí)間: 2025-3-29 16:37
Historical DNA Manipulation Overviewected into mouse embryos at different preimplantation stages. Subsequently, simple DNA plasmids were used to microinject into the pronuclei of fertilized mouse oocytes and that method became the reference for many years. The isolation of embryonic stem cells together with advances in genetics allowe作者: endarterectomy 時(shí)間: 2025-3-29 21:45 作者: 注意 時(shí)間: 2025-3-30 02:58 作者: 狼群 時(shí)間: 2025-3-30 07:41
Modifying Bacterial Artificial Chromosomes for Extended Genome Modificationction expendable, the available BAC libraries are a precious source for life science. Their comprising of extended genomic regions provides an ideal basis for creating a large targeting vector. Here, we describe the identification of suitable BACs from their libraries and their verification prior to作者: Oafishness 時(shí)間: 2025-3-30 08:24 作者: 陪審團(tuán)每個(gè)人 時(shí)間: 2025-3-30 14:07 作者: 青春期 時(shí)間: 2025-3-30 18:51
Microinjection of Zygotes for CRISPR/Cas9-Mediated Insertion of Transgenes into the Murine Rosa26 Saotherwise be addressed in-vitro. Microinjection of zygotes remains the most common technique to generate GM animals to date. Here, we describe the targeted insertion (knock-in) of transgenes by microinjection of 1-cell or 2-cell stage embryos into the murine Rosa26 safe harbor.作者: 膠水 時(shí)間: 2025-3-30 22:45 作者: 一加就噴出 時(shí)間: 2025-3-31 00:56
CRISPR/Cas9 Mutagenesis to Generate Novel Traits in , for Sterile Insect Techniquech as tephritid fruit flies. As SIT is highly favored due to it being species-specific and environmentally friendly, there are constant efforts to improve the efficiency and efficacy of this method in particular at low pest densities; one of which is the use of genetically enhanced strains. Developm
