標(biāo)題: Titlebook: Apoptosis and Cancer; Methods and Protocol Hugo Barcenilla,David Diaz Book 2022 The Editor(s) (if applicable) and The Author(s), under excl [打印本頁] 作者: 猛烈抨擊 時間: 2025-3-21 18:36
書目名稱Apoptosis and Cancer影響因子(影響力)
書目名稱Apoptosis and Cancer影響因子(影響力)學(xué)科排名
書目名稱Apoptosis and Cancer網(wǎng)絡(luò)公開度
書目名稱Apoptosis and Cancer網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Apoptosis and Cancer被引頻次
書目名稱Apoptosis and Cancer被引頻次學(xué)科排名
書目名稱Apoptosis and Cancer年度引用
書目名稱Apoptosis and Cancer年度引用學(xué)科排名
書目名稱Apoptosis and Cancer讀者反饋
書目名稱Apoptosis and Cancer讀者反饋學(xué)科排名
作者: 易怒 時間: 2025-3-21 20:43 作者: Cholagogue 時間: 2025-3-22 03:25
Book 2022, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and practical, .Apoptosis and Cancer: Methods and Protocols. is a valuable resource and practical guide for both novice and expert researchers looking into the “meaning of death.”.作者: Axon895 時間: 2025-3-22 06:58
1064-3745 ation advice from the experts.This volume discusses methods used in the fields of molecular and cellular biology for detecting and studying cell death, especially in cancer and cancer therapy. Chapters in this book cover topics such as non-destructive, real-time Annexin V apoptosis assay; assessment作者: 專橫 時間: 2025-3-22 11:06
F. Fehér,H. Hecht,W. Herr,A. Schneidern on cancer spheroids for the discovery of drugs that could be used in combination with radiotherapy. Device fabrication, preparation, and seeding are also covered. Cell death arising following treatment is detected and characterized according to spheroid size, colony formation assays, and flow cytometry analysis of apoptotic marker annexin V.作者: ANTE 時間: 2025-3-22 14:09
https://doi.org/10.1007/978-3-662-30593-5lysis. The method gives comparable results to established fluorescence markers in several different applications and is, in principle, compatible with any culture format. Given its simplicity, accessibility, and inexpensive nature, the method can complement or provide an alternative to other methods for apoptosis detection.作者: ACME 時間: 2025-3-22 20:39 作者: KEGEL 時間: 2025-3-22 21:49
Apoptosis Detection by Quantification of Cell Debris in Bright-Field Microscopy Images,lysis. The method gives comparable results to established fluorescence markers in several different applications and is, in principle, compatible with any culture format. Given its simplicity, accessibility, and inexpensive nature, the method can complement or provide an alternative to other methods for apoptosis detection.作者: 蝕刻術(shù) 時間: 2025-3-23 01:46 作者: Palliation 時間: 2025-3-23 08:02 作者: Vital-Signs 時間: 2025-3-23 10:38 作者: Accolade 時間: 2025-3-23 16:58 作者: 防銹 時間: 2025-3-23 18:31
Cell Cycle Analysis of ER Stress and Autophagy, or specifically reticulophagy occurs. However, LC3B, PERK, protein misfolding, and changes in ER mass (reticulophagy) can also be measured in a cell cycle-dependent manner by flow cytometry and the use of antibodies, protein misfolding, and ER tracking fluorescent probes.作者: 休息 時間: 2025-3-23 23:51
Simultaneous Detection of Inflammasome Activation and Membrane Damage During Pyroptosis,ly detect two hallmarks of inflammasome-mediated pyroptosis. Using a fluorescently tagged inflammasome adaptor protein (ASC-Citrine) and membrane-impermeable nuclear dyes, we can track inflammasome formation and plasma membrane disruption over time in the same cell population.作者: Expiration 時間: 2025-3-24 03:56 作者: 墻壁 時間: 2025-3-24 09:02
Ueber die Bedingungen des Gleichgewichts,tic (cell death) protein machinery at a single-cell level. We provide a comprehensive overview of a tailor-made set of cell survival/death antibodies, ideal staining conditions, and high-dimensional data analysis.作者: 監(jiān)禁 時間: 2025-3-24 11:44 作者: 笨拙的我 時間: 2025-3-24 17:32
Bausteinorientierte Synthesestrategie,s chapter, we provide a detailed protocol for performing CyTOF phosphoflow in human whole blood, using cytokines and other stimuli. Barcoding and combining of multiple samples and other techniques to reduce batch effects and provide optimal comparability between samples/stimulations are also described.作者: 一個攪動不安 時間: 2025-3-24 21:53 作者: Pericarditis 時間: 2025-3-25 01:37 作者: 敲詐 時間: 2025-3-25 05:56
Elemente der Technologischen Mechanikly detect two hallmarks of inflammasome-mediated pyroptosis. Using a fluorescently tagged inflammasome adaptor protein (ASC-Citrine) and membrane-impermeable nuclear dyes, we can track inflammasome formation and plasma membrane disruption over time in the same cell population.作者: neutral-posture 時間: 2025-3-25 10:46
Apoptosis and Cancer978-1-0716-2553-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 馬賽克 時間: 2025-3-25 15:19
https://doi.org/10.1007/978-3-662-40293-1calization of these interactions. PLISA can be used to quantify autophagy flux and can as well be adapted to assess global autophagy (SQSTM1/P62-LC3B interaction) or specific autophagy, such as mitophagy (NIX-LC3B). Here, we describe a step-by-step method to monitor autophagy using PLISA in adherent cancer cells.作者: 誤傳 時間: 2025-3-25 17:52 作者: 昏睡中 時間: 2025-3-25 21:06
Methods in Molecular Biologyhttp://image.papertrans.cn/a/image/159003.jpg作者: OATH 時間: 2025-3-26 00:21
https://doi.org/10.1007/978-3-662-30593-5 real-time functionality allows for temporal resolution of apoptotic and cell death responses during the test exposure and obviates the need for onerous sample preparation and time course protocols associated with other annexin V methods. Further, this technique is eminently accessible to a wide ran作者: 慎重 時間: 2025-3-26 06:09 作者: dermatomyositis 時間: 2025-3-26 12:15 作者: Small-Intestine 時間: 2025-3-26 16:25
Elemente der Siebenten Hauptgruppe Iapoptotic cells displaying a specific linage antigen (LAg) within a population of cells that remain unfragmented and retain the expression of the LAg. However, this approach has two major limitations. Firstly, apoptotic cells fragment into apoptotic bodies that later disintegrate. Secondly, apoptoti作者: 相容 時間: 2025-3-26 18:57
Elemente der Siebenten Hauptgruppe IIDuring efferocytosis, phagocytes are recruited to the site/activated by “find me” signals released from apoptotic cells, precisely identify apoptotic cells by the recognition of “eat me” signals on the apoptotic cell surface, and engulf the apoptotic cells to prevent secondary necrosis and inflammat作者: constitutional 時間: 2025-3-26 23:53
Elemente der Siebenten Hauptgruppe IIemploying a predefined form of active signaling without the release of soluble cytoplasmic contents to the external environment. Compared to apoptosis, necrosis is a nonspecific form of sudden cell death in response to an invasive external stimulus which in turn is devoid of active programmed intrac作者: PATRI 時間: 2025-3-27 02:43 作者: 音的強(qiáng)弱 時間: 2025-3-27 07:06
Ueber die Bedingungen des Gleichgewichts,easurement of >50 protein moieties on the surface and inside the cell. The power of CyTOF lies in the application of purpose-built panels of antibody probes that resolve features of key biological processes in a cell. Here, we describe this technology’s use to profile changes in the intrinsic apopto作者: 爭論 時間: 2025-3-27 09:53
,Am Molekülgerüst orientierte Bindungss?tze, of technologies that would facilitate feasible and relatively cheap profiling of such a number of cells with an antibody-based approach led us to the development of a high-throughput cytometry-based platform for surface profiling. We coupled the fluorescent cell barcoding with preexisting, commerci作者: 整潔 時間: 2025-3-27 16:43
Bausteinorientierte Synthesestrategie, Extension of this technique to mass cytometry (cytometry by time-of-flight or CyTOF) allows many more cell phenotypes and signaling nodes to be interrogated in parallel. The use of fresh whole blood is ideal for capturing the in vivo signaling state of all leukocytes, including granulocytes. In thi作者: 隱士 時間: 2025-3-27 20:47
Elemente der Technologischen Mechanikey roles in the pathogenesis of cancer and autoimmune diseases through multiple mechanisms including the formation of neutrophil extracellular traps (NETs). Further research to expand the understanding of neutrophils’ role in health and diseases is limited by lack of techniques to quantify neutrophi作者: cogent 時間: 2025-3-28 01:05
Elemente der Technologischen Mechanikt in electrophilic metabolites which in turn induce protein carbonylations. Identification of specific carbonylated proteins and sites in ferroptotic cells will be of great significance for understanding the mechanism and discovering potential biomarkers for this new cell death. The protocol describ作者: Dysarthria 時間: 2025-3-28 05:13 作者: Choreography 時間: 2025-3-28 06:46 作者: 認(rèn)識 時間: 2025-3-28 13:47 作者: FLINT 時間: 2025-3-28 16:46 作者: Detoxification 時間: 2025-3-28 22:35
https://doi.org/10.1007/978-1-0716-2553-8Live-Content Imaging; Cell Imaging; microfluidic flow cytometry (μFCM); cell lysates; epithelial cells s作者: 小鹿 時間: 2025-3-29 00:16
978-1-0716-2555-2The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines作者: 糾纏 時間: 2025-3-29 03:20
Proximity Ligation in Situ Assay to Monitor Autophagy-Related Protein Interactions and Autophagy incalization of these interactions. PLISA can be used to quantify autophagy flux and can as well be adapted to assess global autophagy (SQSTM1/P62-LC3B interaction) or specific autophagy, such as mitophagy (NIX-LC3B). Here, we describe a step-by-step method to monitor autophagy using PLISA in adherent cancer cells.作者: 眨眼 時間: 2025-3-29 07:42 作者: 刺激 時間: 2025-3-29 14:11 作者: 安撫 時間: 2025-3-29 15:59 作者: LIMN 時間: 2025-3-29 20:16
Accurate Enumeration of Apoptotic Cancer Cells Using Flow Cytometry,apoptotic cells displaying a specific linage antigen (LAg) within a population of cells that remain unfragmented and retain the expression of the LAg. However, this approach has two major limitations. Firstly, apoptotic cells fragment into apoptotic bodies that later disintegrate. Secondly, apoptoti作者: 柏樹 時間: 2025-3-30 02:57 作者: 有組織 時間: 2025-3-30 04:18
Quantitative Analysis of Apoptosis and Necrosis in Live Cells Using Flow Cytometry,employing a predefined form of active signaling without the release of soluble cytoplasmic contents to the external environment. Compared to apoptosis, necrosis is a nonspecific form of sudden cell death in response to an invasive external stimulus which in turn is devoid of active programmed intrac作者: 儲備 時間: 2025-3-30 10:08 作者: pulmonary 時間: 2025-3-30 14:22 作者: CODE 時間: 2025-3-30 18:58
High-Throughput, Parallel Flow Cytometry Screening of Hundreds of Cell Surface Antigens Using Fluor of technologies that would facilitate feasible and relatively cheap profiling of such a number of cells with an antibody-based approach led us to the development of a high-throughput cytometry-based platform for surface profiling. We coupled the fluorescent cell barcoding with preexisting, commerci作者: 過濾 時間: 2025-3-30 22:10
Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood, Extension of this technique to mass cytometry (cytometry by time-of-flight or CyTOF) allows many more cell phenotypes and signaling nodes to be interrogated in parallel. The use of fresh whole blood is ideal for capturing the in vivo signaling state of all leukocytes, including granulocytes. In thi作者: 不知疲倦 時間: 2025-3-31 02:08
Quantification of Neutrophils Undergoing NET Formation and Distinguishing Mechanisms of Neutrophil ey roles in the pathogenesis of cancer and autoimmune diseases through multiple mechanisms including the formation of neutrophil extracellular traps (NETs). Further research to expand the understanding of neutrophils’ role in health and diseases is limited by lack of techniques to quantify neutrophi作者: 固執(zhí)點(diǎn)好 時間: 2025-3-31 08:03 作者: IRK 時間: 2025-3-31 13:11
Cell Cycle Analysis of ER Stress and Autophagy,tress sensor signaling proteins PERK (protein kinase R-like endoplasmic reticulum kinase), IRE1, and ATF6 which initiate protein refolding and elongation of the ER until ER homeostasis is returned. If the misfolding of proteins is increased, then ER stress is maintained, and microautophagy of the ER作者: 音樂會 時間: 2025-3-31 17:13
Proximity Ligation in Situ Assay to Monitor Autophagy-Related Protein Interactions and Autophagy incalization of these interactions. PLISA can be used to quantify autophagy flux and can as well be adapted to assess global autophagy (SQSTM1/P62-LC3B interaction) or specific autophagy, such as mitophagy (NIX-LC3B). Here, we describe a step-by-step method to monitor autophagy using PLISA in adherent
