標(biāo)題: Titlebook: Antiviral Resistance in Plants; Methods and Protocol Kappei Kobayashi,Masamichi Nishiguchi Book 2019 Springer Science+Business Media, LLC, [打印本頁(yè)] 作者: Strategy 時(shí)間: 2025-3-21 18:39
書(shū)目名稱(chēng)Antiviral Resistance in Plants影響因子(影響力)
書(shū)目名稱(chēng)Antiviral Resistance in Plants影響因子(影響力)學(xué)科排名
書(shū)目名稱(chēng)Antiviral Resistance in Plants網(wǎng)絡(luò)公開(kāi)度
書(shū)目名稱(chēng)Antiviral Resistance in Plants網(wǎng)絡(luò)公開(kāi)度學(xué)科排名
書(shū)目名稱(chēng)Antiviral Resistance in Plants被引頻次
書(shū)目名稱(chēng)Antiviral Resistance in Plants被引頻次學(xué)科排名
書(shū)目名稱(chēng)Antiviral Resistance in Plants年度引用
書(shū)目名稱(chēng)Antiviral Resistance in Plants年度引用學(xué)科排名
書(shū)目名稱(chēng)Antiviral Resistance in Plants讀者反饋
書(shū)目名稱(chēng)Antiviral Resistance in Plants讀者反饋學(xué)科排名
作者: 責(zé)問(wèn) 時(shí)間: 2025-3-21 20:49 作者: Override 時(shí)間: 2025-3-22 02:19 作者: 咯咯笑 時(shí)間: 2025-3-22 05:17 作者: 披肩 時(shí)間: 2025-3-22 12:31
Book 2019thoritative and practical, .Antiviral Resistance in Plants: Methods and Protocols. serves as an ideal guide for researchers working to combat the serious threats plant virus diseases represent for agricultural production and beyond..作者: 吞下 時(shí)間: 2025-3-22 14:18 作者: tic-douloureux 時(shí)間: 2025-3-22 19:05
Shefali Virkar,Gabriela Viale Pereirastriction sites to find out the sites, in which the insertion does not affect the protein function. Epitope tags are inserted into the experimentally selected sites to produce a functional protein with an epitope tag.作者: entrance 時(shí)間: 2025-3-22 23:16 作者: Aggrandize 時(shí)間: 2025-3-23 02:28
https://doi.org/10.1007/978-3-319-44421-5ication of high quality RNA to guidance for library preparation and sequencing strategies. We report a series of free available tools and programs as well as in-house Perl scripts to perform customized sRNA-seq data mining. Previous bioinformatic background is not required, but experience with basic Unix commands is desirable.作者: aptitude 時(shí)間: 2025-3-23 08:02
Lecture Notes in Computer Sciencemids via ., and tested for virus resistance. Actually, the T2 and T3 plants showed resistance to tomato mosaic virus. Here we describe the method to construct RNA silencing-inducing plasmids, to transform tomato plants and to check the introduction of transgenes and virus resistance.作者: 諂媚于性 時(shí)間: 2025-3-23 11:00
Ansgar Mondorf,Maria A. Wimmer,Daniel Reisericiency and distribution in a plant based on immunostaining results. In addition, a practical inoculation method that uses a spray gun to apply attenuated viruses to a large number of seedlings is presented.作者: 虛假 時(shí)間: 2025-3-23 14:01 作者: COM 時(shí)間: 2025-3-23 21:48 作者: 通情達(dá)理 時(shí)間: 2025-3-23 23:11
Computational Workflow for Small RNA Profiling in Virus-Infected Plants,ication of high quality RNA to guidance for library preparation and sequencing strategies. We report a series of free available tools and programs as well as in-house Perl scripts to perform customized sRNA-seq data mining. Previous bioinformatic background is not required, but experience with basic Unix commands is desirable.作者: synovium 時(shí)間: 2025-3-24 03:55 作者: Deference 時(shí)間: 2025-3-24 07:31 作者: yohimbine 時(shí)間: 2025-3-24 13:17
Ida Lindgren,Csaba Csáki,Anneke Zuiderwijkis chapter, we provide a protocol for evaluating the durability of plant virus resistance gene, which comprises the random mutagenesis of a virus gene, the introduction of the mutagenized gene into a virus context with highly efficient inoculation system, and the efficient screening of virus mutants that can overcome or escape a virus resistance.作者: 幾何學(xué)家 時(shí)間: 2025-3-24 15:19
Ida Lindgren,Csaba Csáki,Anneke Zuiderwijkgenomic RNA of positive-strand RNA viruses using the extract of evacuolated protoplasts. We also describe a method for the identification and functional characterization of a plant resistance gene product using this system.作者: Lymphocyte 時(shí)間: 2025-3-24 21:35
1064-3745 expertsThis detailed book explores strategies that have been developed to combat plant virus infection. Beginning with a section on techniques for identifying and studying the virus resistance gene involved in plant innate immunity, the volume continues by delving into techniques related to novel m作者: guardianship 時(shí)間: 2025-3-25 00:50 作者: neutralize 時(shí)間: 2025-3-25 04:13
Marcelo Fornazin,Luiz Antonio Joiags-CaM (rCML) in . and other Solanaceae plants, including potato (.). To analyze the functions of each rCML, mutations were introduced into potato rCMLs using the CRISPR/Cas9 system. Here, we describe our protocol of the CRISPR/Cas9-mediated targeted mutagenesis in stably transformed potato plants.作者: 破布 時(shí)間: 2025-3-25 08:03 作者: BUOY 時(shí)間: 2025-3-25 12:37
CRISPR/Cas9-Mediated Editing of Genes Encoding rgs-CaM-like Proteins in Transgenic Potato Plants,gs-CaM (rCML) in . and other Solanaceae plants, including potato (.). To analyze the functions of each rCML, mutations were introduced into potato rCMLs using the CRISPR/Cas9 system. Here, we describe our protocol of the CRISPR/Cas9-mediated targeted mutagenesis in stably transformed potato plants.作者: Junction 時(shí)間: 2025-3-25 17:46 作者: incontinence 時(shí)間: 2025-3-25 23:01 作者: 隱士 時(shí)間: 2025-3-26 02:58
Hong Joo Lee,Minsik Lee,Habin Leeolved have been genetically or biochemically identified in .. One powerful tool to dissect antiviral immune systems involves a reverse genetic approach that analyzes . mutant lines with impaired antiviral defense responses. In particular, to better understand the signaling networks involved in the r作者: 商議 時(shí)間: 2025-3-26 05:51
Hanne H?glund Rydén,Sara Hofmann,Guri Vernenisms has been extensively promoted by virus-induced gene silencing (VIGS) technique, which decreases the transcriptional level of the gene of interest via RNA silencing machinery triggered by the partial gene fragment inserted into a viral vector. This technique has contributed to addressing the fu作者: Gobble 時(shí)間: 2025-3-26 11:38
Ida Lindgren,Csaba Csáki,Anneke Zuiderwijk viruses often results in the breakdown of virus resistance in plants, which leads to a disastrous outcome in agricultural production. Although studies have analyzed the nature of plant virus resistance breakdown, it is still difficult to foresee the breakdown of a given virus resistance gene. In th作者: convert 時(shí)間: 2025-3-26 13:54
Ida Lindgren,Csaba Csáki,Anneke Zuiderwijknt tissues or cells. Thus, with a few exceptions, plant cell extracts are contaminated by vacuole-derived lytic enzymes and unsuitable for biochemical analyses. Here, we describe a method for removing the vacuoles from intact tobacco BY-2 protoplasts and for cell-free translation and replication of 作者: 舞蹈編排 時(shí)間: 2025-3-26 18:27 作者: monochromatic 時(shí)間: 2025-3-26 22:53 作者: garrulous 時(shí)間: 2025-3-27 02:42 作者: nauseate 時(shí)間: 2025-3-27 05:33 作者: 個(gè)人長(zhǎng)篇演說(shuō) 時(shí)間: 2025-3-27 11:30
https://doi.org/10.1007/978-3-319-44421-5ns in plants. Our workflow is primarily focused on the analysis of sRNA populations derived from known or previously undescribed viruses infecting host plants. Furthermore, we provide an additional pipeline to examine host-specific endogenous sRNAs activated or specifically expressed during viral in作者: 歸功于 時(shí)間: 2025-3-27 14:05 作者: antenna 時(shí)間: 2025-3-27 21:04
Lecture Notes in Computer Sciencehave been broadly used to confer antiviral resistance in plants. However, methods for designing, synthesizing and functionally analyzing antiviral artificial sRNAs have not been optimized for time and cost-effectiveness and high-throughput applicability since recently. Here we present a systematic m作者: 路標(biāo) 時(shí)間: 2025-3-27 22:49 作者: spinal-stenosis 時(shí)間: 2025-3-28 04:55 作者: Fortuitous 時(shí)間: 2025-3-28 09:28
María Elena López Reyes,Rikke Magnussenrises a bisegmented, single-stranded RNA genome (RNA1 and RNA2) and three different capsid proteins (Vp25, Vp20, and Vp24). The virus can experimentally infect a broad range of plants including, not only model plants (. and . species) but also economically important crops such as cucumber, soybean, 作者: lesion 時(shí)間: 2025-3-28 11:25 作者: LUMEN 時(shí)間: 2025-3-28 14:50
Ulf Melin,Karin Axelsson,Truls L?fstedtnse (HR). Such .-mediated transient gene expression methods have contributed to the identification of new plant . genes and facilitated the analysis of their functions. Here we describe a model method, by which several tobamovirus . genes from Solanaceous plants have been successfully identified and characterized molecularly.作者: AVANT 時(shí)間: 2025-3-28 22:41
Kappei Kobayashi,Masamichi NishiguchiIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts作者: diskitis 時(shí)間: 2025-3-29 02:18
Methods in Molecular Biologyhttp://image.papertrans.cn/a/image/158710.jpg作者: RLS898 時(shí)間: 2025-3-29 03:59
Identification and Functional Analysis of NB-LRR-Type Virus Resistance Genes: Overview and Functionnse (HR). Such .-mediated transient gene expression methods have contributed to the identification of new plant . genes and facilitated the analysis of their functions. Here we describe a model method, by which several tobamovirus . genes from Solanaceous plants have been successfully identified and characterized molecularly.作者: 火車(chē)車(chē)輪 時(shí)間: 2025-3-29 07:49 作者: 陰謀 時(shí)間: 2025-3-29 14:08 作者: 小溪 時(shí)間: 2025-3-29 18:36
Hanne H?glund Rydén,Sara Hofmann,Guri Verne but is not associated with any severe symptoms. Using this method together with inoculation of a challenge virus and transient overexpression of a viral elicitor or another host signaling component by agroinfiltration, as well as measurement of hallmarks of antiviral responses, we can address the r作者: figurine 時(shí)間: 2025-3-29 20:52
https://doi.org/10.1007/978-3-319-44421-5ide broad, durable viral resistance, as it can target conserved regions of the viral genome and can also be customized to emerging viral variants. Moreover, this method can be used in many crop species, as it requires little or no knowledge of the host plant’s genome.作者: Brain-Waves 時(shí)間: 2025-3-30 01:13
Catherine G. Mkude,Maria A. Wimmerf CMV-?2b, preparation of the mutant plants for virus inoculation, identification and characterization of . mutants, and cloning of the genes responsible for the mutant phenotype by either the genetic linkage to T-DNA insertion or a mapping-by-sequencing approach.作者: angina-pectoris 時(shí)間: 2025-3-30 05:57
Lecture Notes in Computer Science molecular analysis of virus accumulation in plant tissues. This approach is aimed to easily identify artificial sRNAs with high antiviral activity that could be expressed in transgenic plants for highly durable antiviral resistance.作者: giggle 時(shí)間: 2025-3-30 10:28
María Elena López Reyes,Rikke Magnussenartial genome sequence of pathogenic viruses display a high degree of cross-protection against the challenge inoculation of the corresponding pathogenic viruses. Treatment effects can also be expected in virus-infected plants by subsequent inoculation with ALSV vaccine.作者: RAG 時(shí)間: 2025-3-30 14:11
1064-3745 lls.?.Authoritative and practical, .Antiviral Resistance in Plants: Methods and Protocols. serves as an ideal guide for researchers working to combat the serious threats plant virus diseases represent for agricultural production and beyond..978-1-4939-9637-7978-1-4939-9635-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 發(fā)展 時(shí)間: 2025-3-30 17:59 作者: Perineum 時(shí)間: 2025-3-30 22:35
