標(biāo)題: Titlebook: Antiviral Resistance in Plants; Methods and Protocol John M. Watson,Ming-Bo Wang Book 2012 Springer Science+Business Media, LLC 2012 Antivi [打印本頁(yè)] 作者: osteomalacia 時(shí)間: 2025-3-21 18:25
書目名稱Antiviral Resistance in Plants影響因子(影響力)
書目名稱Antiviral Resistance in Plants影響因子(影響力)學(xué)科排名
書目名稱Antiviral Resistance in Plants網(wǎng)絡(luò)公開度
書目名稱Antiviral Resistance in Plants網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Antiviral Resistance in Plants被引頻次
書目名稱Antiviral Resistance in Plants被引頻次學(xué)科排名
書目名稱Antiviral Resistance in Plants年度引用
書目名稱Antiviral Resistance in Plants年度引用學(xué)科排名
書目名稱Antiviral Resistance in Plants讀者反饋
書目名稱Antiviral Resistance in Plants讀者反饋學(xué)科排名
作者: Folklore 時(shí)間: 2025-3-21 21:59 作者: lobster 時(shí)間: 2025-3-22 00:51 作者: commute 時(shí)間: 2025-3-22 07:59 作者: 彎曲道理 時(shí)間: 2025-3-22 12:16 作者: 感激小女 時(shí)間: 2025-3-22 14:19
Strategies for Viral Cross Protection in Plants,the mild virus strain to establish MSCP. To investigate interference among virus strains and the plant host at an early stage of MSCP at a subcellular level, we developed a rapid micro-extraction method for the preparation of total nucleic acid (TNA), combined with other molecular methods, to monito作者: artifice 時(shí)間: 2025-3-22 18:33
Plant Viroids: Isolation, Characterization/Detection, and Analysis,red approximately 10 years later, and much effort has subsequently been devoted to simplifying the sample preparation procedure and identifying group-specific primer pairs. This chapter describes four simple, easy-to-follow protocols—two involving PAGE and two others based on enzymatic amplification作者: Paleontology 時(shí)間: 2025-3-23 00:43 作者: 注意到 時(shí)間: 2025-3-23 05:04
Peter Kuhn,Matthias Buchinger,Dian Balta gene regulation systems. From the very beginning, plant research has had a major impact on our understanding of RNAi. The purpose of this chapter is to provide an historical perspective and overview on the discovery, characterization, and applications of RNAi in plants.作者: Semblance 時(shí)間: 2025-3-23 07:17
Lecture Notes in Computer Scienceornamental and plant biotechnology, vol. III, 2006). Four distinct RNA silencing pathways have been recognized in plants, albeit with considerable conservation of the molecular components. These pathways are directed by various small RNA species, including microRNAs (miRNAs), .-acting small interfer作者: 摘要記錄 時(shí)間: 2025-3-23 10:49 作者: 嘲笑 時(shí)間: 2025-3-23 17:41
Hans J. Scholl,Eric E. Holdemanthe mild virus strain to establish MSCP. To investigate interference among virus strains and the plant host at an early stage of MSCP at a subcellular level, we developed a rapid micro-extraction method for the preparation of total nucleic acid (TNA), combined with other molecular methods, to monito作者: 老人病學(xué) 時(shí)間: 2025-3-23 19:24
Towards Interoperable Open Statistical Datared approximately 10 years later, and much effort has subsequently been devoted to simplifying the sample preparation procedure and identifying group-specific primer pairs. This chapter describes four simple, easy-to-follow protocols—two involving PAGE and two others based on enzymatic amplification作者: Derogate 時(shí)間: 2025-3-24 00:18
Creating Local Government Innovatione assignment of functions to specific genes. The strategy involves inserting short sequences of target soybean genes into the viral genome to generate recombinant vectors. Soybean plants are then inoculated with the recombinant vectors, assessed for BPMV propagation and silencing of the target genes作者: Habituate 時(shí)間: 2025-3-24 05:08 作者: ineptitude 時(shí)間: 2025-3-24 09:46 作者: 免除責(zé)任 時(shí)間: 2025-3-24 12:19 作者: 譏笑 時(shí)間: 2025-3-24 15:41
Alexander van Deursen,Jan van DijkThe goal of this chapter is to describe in simple terms how the use of ordinary differential equation (ODE) modeling, in conjunction with experimentation, can be utilized to improve our understanding of the dynamics of gene silencing and virus resistance in plants.作者: nerve-sparing 時(shí)間: 2025-3-24 23:04 作者: 導(dǎo)師 時(shí)間: 2025-3-24 23:17 作者: HUMID 時(shí)間: 2025-3-25 05:16 作者: 愛得痛了 時(shí)間: 2025-3-25 11:11
Peter Kuhn,Matthias Buchinger,Dian Baltare it was called post-transcriptional gene silencing, PTGS) RNAi is a gene down-regulation mechanism since demonstrated to exist in all eukaryotes. In RNAi, small RNAs (of about 21–24 nucleotides) function to guide specific effector proteins (members of the Argonaute protein family) to a target nucl作者: 精美食品 時(shí)間: 2025-3-25 14:20 作者: 就職 時(shí)間: 2025-3-25 16:33
Hans J. Scholl,Eric E. Holdemane molecular immune mechanism against invading viruses. The antiviral RNAi pathway is initiated with the generation of virus-derived short-interfering RNAs (siRNAs) that are used for subsequent sequence-specific recognition and degradation of the cognate viral RNA molecules. As an efficient counter-d作者: 負(fù)擔(dān) 時(shí)間: 2025-3-25 21:29
Peter Kuhn,Matthias Buchinger,Dian Baltaus that has had its genome modified to include a sequence from the host gene to be silenced. DNAβ and DNA1 are satellite and single-stranded DNA molecules associated with begomoviruses (family .). We converted DNAβ and DNA1 into gene-silencing vectors. The VIGS vectors can induce silencing efficient作者: Preamble 時(shí)間: 2025-3-26 03:22
Hans J. Scholl,Eric E. Holdemanmage caused by a severe challenge strain/isolate of the same virus. Mild strain cross protection (MSCP) has been used extensively to control losses caused by a few major virus diseases in some parts of the world. So far, none of the many proposed mechanisms can fully explain the intact process of MS作者: 陰謀小團(tuán)體 時(shí)間: 2025-3-26 07:41
Stijn Wouters,Marijn Janssen,Joep Crompvoetsrigger the host plant’s RNA-silencing machinery and ultimately become the target of RNA silencing. PTGS is, therefore, an attractive endogenous process that can be exploited to study gene function. One of the most efficient approaches of initiating PTGS is through virus-induced gene silencing (VIGS)作者: medieval 時(shí)間: 2025-3-26 08:30 作者: Guileless 時(shí)間: 2025-3-26 15:00 作者: jovial 時(shí)間: 2025-3-26 19:11 作者: Munificent 時(shí)間: 2025-3-26 23:58 作者: brother 時(shí)間: 2025-3-27 05:00 作者: Foreknowledge 時(shí)間: 2025-3-27 08:18
Panos Panagiotopoulos,Frances Bowenng that leads to high-level virus resistance is triggered by virus-specific double-stranded (ds)RNA. The most efficient means to produce such dsRNA in transgenic plants is the introduction and expression of hairpin (hp) RNA constructs. Successful induction of the RNA silencing pathway is witnessed b作者: Soliloquy 時(shí)間: 2025-3-27 11:48 作者: brassy 時(shí)間: 2025-3-27 14:34
Lecture Notes in Computer Science three methods for extracting small RNA from cells: a general method, one plant specific and a third particular to conifers. Further, protocols are given for the analysis and quantification of small RNAs using polyacrylamide gel-based approaches. A native streptavidin gel-shift assay, useful for mea作者: 不容置疑 時(shí)間: 2025-3-27 21:34
Florian Evéquoz,Hugo Castanheirohas been associated with the synthesis of short interfering RNAs (siRNAs), which are essential regulators of genomic integrity and plant viral defense. The multiple gene copies, and functional diversities, of the plant RDRPs raise the question of whether their intrinsic properties differ. This chapt作者: 顛簸地移動(dòng) 時(shí)間: 2025-3-28 01:40 作者: 大炮 時(shí)間: 2025-3-28 02:31 作者: guardianship 時(shí)間: 2025-3-28 07:57 作者: chemoprevention 時(shí)間: 2025-3-28 10:56 作者: Irascible 時(shí)間: 2025-3-28 16:15
RNA Silencing and Antiviral Defense in Plants,. Since the publication of landmark papers in 1998 (Fire et al., Nature 391:806–811, 1998; Waterhouse et al., Proc Natl Acad Sci U S A 95:13959–13964, 1998), intense research efforts have resulted in much progress from the speculation of Mello and colleagues that “the mechanisms underlying RNA inter作者: BILE 時(shí)間: 2025-3-28 20:13
Biological Chemistry of Virus-Encoded Suppressors of RNA Silencing: An Overview,e molecular immune mechanism against invading viruses. The antiviral RNAi pathway is initiated with the generation of virus-derived short-interfering RNAs (siRNAs) that are used for subsequent sequence-specific recognition and degradation of the cognate viral RNA molecules. As an efficient counter-d作者: 就職 時(shí)間: 2025-3-29 00:51 作者: 閃光東本 時(shí)間: 2025-3-29 06:01 作者: nocturia 時(shí)間: 2025-3-29 08:01 作者: Amendment 時(shí)間: 2025-3-29 12:57 作者: CROAK 時(shí)間: 2025-3-29 17:46 作者: PAC 時(shí)間: 2025-3-29 23:08
Generation of Plant Small RNA cDNA Libraries for High-Throughput Sequencing,in plants. Conventional cloning and sequencing approaches have identified hundreds of miRNAs and a large number of siRNAs, but are no longer the best choices for identification of new miRNAs which are generally expressed at low abundance. The development of next-generation sequencing technologies ha作者: Stricture 時(shí)間: 2025-3-30 03:11
Isolation and Detection of Small RNAs from Plant Tissues,l processes. The two main classes of sRNA, the small-interfering RNA (siRNA) and microRNA (miRNA) classes, are well documented and several experimental approaches have been developed to allow for their routine isolation and detection from plant tissues. Here, we describe the current methods used for作者: 有偏見 時(shí)間: 2025-3-30 05:10 作者: Hay-Fever 時(shí)間: 2025-3-30 09:19
Use of Hairpin RNA Constructs for Engineering Plant Virus Resistance,ng that leads to high-level virus resistance is triggered by virus-specific double-stranded (ds)RNA. The most efficient means to produce such dsRNA in transgenic plants is the introduction and expression of hairpin (hp) RNA constructs. Successful induction of the RNA silencing pathway is witnessed b作者: 凹處 時(shí)間: 2025-3-30 15:08 作者: 大都市 時(shí)間: 2025-3-30 17:08 作者: 法官 時(shí)間: 2025-3-30 22:34 作者: 破譯密碼 時(shí)間: 2025-3-31 03:49 作者: agnostic 時(shí)間: 2025-3-31 07:15 作者: ear-canal 時(shí)間: 2025-3-31 11:59
Virus-Induced Gene Silencing in Soybean,d genomic tools are available for studies in the crop plant soybean, investigations related to gene function have been lacking. This is largely due to the fact that rapid functional genomics tools have been unavailable for application in soybean. This constraint was recently alleviated when a novel 作者: 嚴(yán)峻考驗(yàn) 時(shí)間: 2025-3-31 15:36
Book 2012ed to the vital discovery of double-stranded RNA (dsRNA)-induced gene silencing or RNA interference (RNAi), which has since revolutionized research on plant-virus interactions. In .Antiviral Resistance in Plants: Methods and Protocols., expert researchers in the field detail many of the methods whic作者: 突變 時(shí)間: 2025-3-31 19:49 作者: Estrogen 時(shí)間: 2025-4-1 00:26
Long Wang,Justus Bross,Christoph Meinelan agroinfiltration assay and grafting experiments, which were used for the characterization of . 50 kDa movement protein as a gene silencing suppressor. This protocol should allow the investigator to characterize putative plant virus-encoded gene silencing suppressors.作者: 使?jié)M足 時(shí)間: 2025-4-1 05:55 作者: Instinctive 時(shí)間: 2025-4-1 09:55
