標題: Titlebook: Antisense RNA Design, Delivery, and Analysis; Virginia Arechavala-Gomeza,Alejandro Garanto Book‘‘‘‘‘‘‘‘ 2022 The Editor(s) (if applicable) [打印本頁] 作者: Hayes 時間: 2025-3-21 18:11
書目名稱Antisense RNA Design, Delivery, and Analysis影響因子(影響力)
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書目名稱Antisense RNA Design, Delivery, and Analysis網(wǎng)絡(luò)公開度學科排名
書目名稱Antisense RNA Design, Delivery, and Analysis被引頻次
書目名稱Antisense RNA Design, Delivery, and Analysis被引頻次學科排名
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書目名稱Antisense RNA Design, Delivery, and Analysis年度引用學科排名
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書目名稱Antisense RNA Design, Delivery, and Analysis讀者反饋學科排名
作者: gain631 時間: 2025-3-22 00:07 作者: 枯萎將要 時間: 2025-3-22 02:33
2.1.6 References to crystal structure,RNA vaccines against SARS-CoV-2. Another type of nucleic acid therapeutics is antisense oligonucleotides, versatile tools that can be used in multiple ways to target pre-mRNA and mRNA. While some years ago these molecules were just considered a useful research tool and a curiosity in the clinical ma作者: 情感 時間: 2025-3-22 06:32 作者: CRACK 時間: 2025-3-22 12:18
2.1.6 References to crystal structure,essary to specifically upregulate target gene translation. Originally identified in the mouse . (antisense Ubiquitin carboxyl-terminal esterase L1) locus, natural SINEUP molecules are oriented head to head to their sense protein coding, target gene (., in this example). Peculiarly, SINEUP is able to作者: Barter 時間: 2025-3-22 16:37 作者: 愚笨 時間: 2025-3-22 17:29 作者: 增減字母法 時間: 2025-3-23 00:22
2.1.1 List of symbols and abbreviations, gene delivery are positively charged to carry negatively charged oligonucleotides. However, excessive positively charged carriers are cytotoxic. Therefore, the complexed oligonucleotide/nanoparticles should be well-examined before the application. In that manner, agarose gel electrophoresis, which 作者: 合法 時間: 2025-3-23 01:27
2.1.1 List of symbols and abbreviations,gh stability and affinity for target sequences. However, in spite of their neutral charge as compared to natural oligonucleotides or phosphorothioate analogs, they still show little permeability for cellular membranes, highlighting the need for effective cytosolic delivery strategies. In addition, t作者: 協(xié)奏曲 時間: 2025-3-23 08:19 作者: chronology 時間: 2025-3-23 11:31 作者: clarify 時間: 2025-3-23 16:01
2.1.5 References for the introduction,basement membrane zone, C7 secures attachment of the epidermal basal keratinocyte to the papillary dermis by means of anchoring fibril formation. The complete absence of these anchoring fibrils leads to severe blistering of skin and mucosa upon the slightest friction and early mortality. To date, al作者: 頑固 時間: 2025-3-23 18:49
2.1.5 References for the introduction, affect its splicing gives AONs potential use for exon skipping therapies aimed at restoring the dystrophin transcript reading frame for Duchenne muscular dystrophy (DMD) patients. The neutrally charged phosphorodiamidate morpholino oligomers (PMOs) are a stable and relatively nontoxic AON modificat作者: heirloom 時間: 2025-3-24 01:56 作者: Palatial 時間: 2025-3-24 03:06 作者: homeostasis 時間: 2025-3-24 08:35
2.1.1 List of symbols and abbreviations,f canonical pre-mRNA splicing by disease-associated variants can result in genetic disorders. Antisense oligonucleotides (AONs) offer an attractive solution to modulate endogenous gene expression through alteration of pre-mRNA splicing events. Relevant in vitro models are crucial for appropriate eva作者: magnate 時間: 2025-3-24 12:52 作者: 恫嚇 時間: 2025-3-24 18:34
https://doi.org/10.1007/978-1-0716-2010-6RNA therapeutics; Antisense technology; Therapeutic design; Oligonucleotides; Model systems; Oligonucleot作者: 恫嚇 時間: 2025-3-24 21:41 作者: 集聚成團 時間: 2025-3-25 00:45 作者: 耕種 時間: 2025-3-25 06:47 作者: LUDE 時間: 2025-3-25 07:37 作者: 腐蝕 時間: 2025-3-25 13:49
2.1.1 List of symbols and abbreviations, we demonstrate that quantitative dot blots in plate format are a better option to determine the absolute contents of a given protein in less than 48?h. The method was optimized for the detection of the Muscleblind-like 1 protein in patient-derived myoblasts treated with a collection of more than 100 experimental oligonucleotides.作者: 防水 時間: 2025-3-25 19:49 作者: FELON 時間: 2025-3-25 23:23 作者: senile-dementia 時間: 2025-3-26 00:18 作者: 流動性 時間: 2025-3-26 07:17
Design of Bifunctional Antisense Oligonucleotides for Exon Inclusionously published are listed. We also present methodology of assessing the effects of TOES on exon inclusion in fibroblasts cultured from a SMA patient. The effects of TOES on . exon 7 splicing were validated at RNA level by PCR and quantitative real-time PCR, and at protein level by western blotting.作者: hieroglyphic 時間: 2025-3-26 12:13
Evaluation of Exon Skipping and Dystrophin Restoration in In Vitro Models of Duchenne Muscular Dystrn this evaluation and describe in detail the protocols routinely followed at our institution, one to evaluate the efficacy of skipping at RNA level (nested PCR) and the other the restoration of protein expression (myoblot), which provide good results using equipment largely available to most research laboratories.作者: floodgate 時間: 2025-3-26 15:58 作者: oblique 時間: 2025-3-26 18:01 作者: 聯(lián)想記憶 時間: 2025-3-26 22:36 作者: disrupt 時間: 2025-3-27 04:00
https://doi.org/10.1007/BFb0111518A development and other applications that have by and large utilized the same modifications. We also point out the pitfalls of the use of nucleic acids as drugs, such as their unwanted interactions with pattern recognition receptors, which can be mitigated by chemical modification or used as immunotherapeutic agents.作者: Modicum 時間: 2025-3-27 07:07
Introduction and History of the Chemistry of Nucleic Acids TherapeuticsA development and other applications that have by and large utilized the same modifications. We also point out the pitfalls of the use of nucleic acids as drugs, such as their unwanted interactions with pattern recognition receptors, which can be mitigated by chemical modification or used as immunotherapeutic agents.作者: 墻壁 時間: 2025-3-27 11:42 作者: ALERT 時間: 2025-3-27 15:39 作者: FIR 時間: 2025-3-27 21:24 作者: RACE 時間: 2025-3-28 01:54 作者: neutrophils 時間: 2025-3-28 04:23 作者: 農(nóng)學 時間: 2025-3-28 08:38 作者: inculpate 時間: 2025-3-28 13:08
Development and Use of Cellular Systems to Assess and Correct Splicing Defectsribe how to engineer splicing vectors, validate the reliability and reproducibility of alternative cellular systems, assess pre-mRNA splicing defects involved in IRD, and finally correct those by using antisense oligonucleotide-based strategies.作者: STENT 時間: 2025-3-28 15:24 作者: observatory 時間: 2025-3-28 21:35
Introduction and History of the Chemistry of Nucleic Acids Therapeuticsapeutics focusing in particular on antisense oligonucleotide analogues carrying modifications in the backbone and sugar. Brief mention is made of siRNA development and other applications that have by and large utilized the same modifications. We also point out the pitfalls of the use of nucleic acid作者: 橡子 時間: 2025-3-29 00:11
Antisense RNA Therapeutics: A Brief OverviewRNA vaccines against SARS-CoV-2. Another type of nucleic acid therapeutics is antisense oligonucleotides, versatile tools that can be used in multiple ways to target pre-mRNA and mRNA. While some years ago these molecules were just considered a useful research tool and a curiosity in the clinical ma作者: 拔出 時間: 2025-3-29 06:57 作者: arthrodesis 時間: 2025-3-29 07:47
Design and Delivery of SINEUP: A New Modular Tool to Increase Protein Translationessary to specifically upregulate target gene translation. Originally identified in the mouse . (antisense Ubiquitin carboxyl-terminal esterase L1) locus, natural SINEUP molecules are oriented head to head to their sense protein coding, target gene (., in this example). Peculiarly, SINEUP is able to作者: 小丑 時間: 2025-3-29 11:40
How to Design U1 snRNA Molecules for Splicing RescueRNA splicing requires the correct identification of a number of .-acting elements in an ordered fashion. By disrupting the complementarity of the 5′ss with the endogenous small nuclear RNA U1 (U1 snRNA), the key component of the spliceosomal U1 ribonucleoprotein, 5′ss mutations may result in exon sk作者: 口味 時間: 2025-3-29 19:00
Conjugation of Nucleic Acids and Drugs to Gold Nanoparticleslization into the cell and prevent their degradation. In addition, engineered AuNPs can be employed as sensors of a variety of biomarkers, where the electronic and optical properties of the AuNPs are exploited for a convenient, easy, and fast read out. However, in all these applications, a key step 作者: Assault 時間: 2025-3-29 23:28
Determination of Optimum Ratio of Cationic Polymers and Small Interfering RNA with Agarose Gel Retar gene delivery are positively charged to carry negatively charged oligonucleotides. However, excessive positively charged carriers are cytotoxic. Therefore, the complexed oligonucleotide/nanoparticles should be well-examined before the application. In that manner, agarose gel electrophoresis, which 作者: Graduated 時間: 2025-3-29 23:57 作者: BILE 時間: 2025-3-30 06:26 作者: entail 時間: 2025-3-30 09:14 作者: 烤架 時間: 2025-3-30 12:36 作者: Bucket 時間: 2025-3-30 19:00
In Vitro Delivery of PMOs in Myoblasts by Electroporation affect its splicing gives AONs potential use for exon skipping therapies aimed at restoring the dystrophin transcript reading frame for Duchenne muscular dystrophy (DMD) patients. The neutrally charged phosphorodiamidate morpholino oligomers (PMOs) are a stable and relatively nontoxic AON modificat作者: affluent 時間: 2025-3-31 00:00 作者: 并入 時間: 2025-3-31 02:38
Evaluation of Exon Skipping and Dystrophin Restoration in In Vitro Models of Duchenne Muscular Dystre muscular dystrophy, but many more are in development targeting an array of different . exons. Preclinical screening of the new oligonucleotide sequences is routinely performed using patient-derived cell cultures, and evaluation of their efficacy may be performed at RNA and/or protein level. While 作者: 你正派 時間: 2025-3-31 08:55
Generation of Human iPSC-Derived Myotubes to Investigate RNA-Based Therapies In Vitrof canonical pre-mRNA splicing by disease-associated variants can result in genetic disorders. Antisense oligonucleotides (AONs) offer an attractive solution to modulate endogenous gene expression through alteration of pre-mRNA splicing events. Relevant in vitro models are crucial for appropriate eva作者: LAIR 時間: 2025-3-31 12:02 作者: 神圣在玷污 時間: 2025-3-31 16:28
2.1.6 References to crystal structure,RNA, synthetic SINEUP molecules have been developed by creating a specific BD for the gene of interest and placing it upstream the invSINEB2 ED. Synthetic SINEUP is thus a novel molecular tool that potentially may be used for any industrial or biomedical application to enhance protein production, al
