作者: STAT 時(shí)間: 2025-3-21 23:08
1.4.2 General features of the data,infectious fungi. These methods include multilocus enzyme electrophoresis, restriction fragment-length polymorphism using complex probes, random amplification of polymorphic DNA, and multilocus sequence typing, which are described in this chapter.作者: AORTA 時(shí)間: 2025-3-22 00:29 作者: 抒情短詩(shī) 時(shí)間: 2025-3-22 05:51
1.4.2 General features of the data, a specific gene to drug resistance is to examine its inactivation in a resistant isolate and to analyze the effect of the mutation on the resistance phenotype. The generation of knockout mutants in the diploid yeast . requires two rounds of gene replacement to inactivate both alleles of a target ge作者: 為敵 時(shí)間: 2025-3-22 12:18 作者: 協(xié)迫 時(shí)間: 2025-3-22 15:59
https://doi.org/10.1007/978-1-4899-1579-5teomics for the study of this fungal pathogen. Proteome analysis includes the separation and isolation of proteins by two-dimensional polyacrylamide gel electrophoresis and subsequent protein identification by peptide mass fingerprinting using matrix-assisted laser desorption ionization time-of-flig作者: Myocyte 時(shí)間: 2025-3-22 20:36
Multiple Inelastic Electron Scatteringferent from their free-floating planktonic counterparts, including their recalcitrance to antimicrobial agents. Consequently, . biofilm-associated infections are difficult to treat and to fully eradicate with standard antifungal therapy. Here, we describe a simple, fast, inexpensive and highly repro作者: Ganglion-Cyst 時(shí)間: 2025-3-23 00:16 作者: 津貼 時(shí)間: 2025-3-23 02:51 作者: 商店街 時(shí)間: 2025-3-23 05:46 作者: 系列 時(shí)間: 2025-3-23 12:43
Mechanics of Elastic and Inelastic Solids that are being diagnosed in severly immunocompromised hosts. In addition, opportunistic fungal infections resistant to antifungal drugs have become increasingly common, and the armamentarium for treatment remains limited. A possible approach to overcoming these problems is to combine antifungal dru作者: Popcorn 時(shí)間: 2025-3-23 15:15
https://doi.org/10.1007/978-94-009-6866-0suspension. In vitro, this effect might simulate the in vivo situation when the concentration of the drug falls to less than the minimum inhibitory concentration values and could be another tool, together with the classic in vitro susceptibility tests, to optimize the interaction of drugs-fungi. In 作者: declamation 時(shí)間: 2025-3-23 18:12
Random media and wave propagation, have been developed and may interact with both polymorphonuclear and mononuclear phagocytes against blastoconidia and pseudohyphae of . spp. Blastoconidia are predominantly destroyed by phagocytosis, whereas pseudohyphal killing is initiated after the attachment of phagocytes to the cell wall of th作者: 猛烈責(zé)罵 時(shí)間: 2025-3-24 01:43 作者: 新字 時(shí)間: 2025-3-24 04:03 作者: 擔(dān)心 時(shí)間: 2025-3-24 10:01
https://doi.org/10.1385/1592599435cytokine; drug; drug discovery; fungal infection; immunology; immunotherapy; infection; infections; molecula作者: 比賽用背帶 時(shí)間: 2025-3-24 11:32 作者: Resistance 時(shí)間: 2025-3-24 17:55
Deoxyribonucleic Acid Fingerprinting Methods for , Speciesinfectious fungi. These methods include multilocus enzyme electrophoresis, restriction fragment-length polymorphism using complex probes, random amplification of polymorphic DNA, and multilocus sequence typing, which are described in this chapter.作者: HAUNT 時(shí)間: 2025-3-24 22:55 作者: Eviction 時(shí)間: 2025-3-25 01:06
1.4.2 General features of the data,mmercial or commercial tests. Fungicidal testing includes either in vitro methods, such as time-kill or minimum fungicidal testing methods, or animal models. This chapter provides background information for utilizing and evaluating results obtained from antifungal susceptibility testing methods.作者: labyrinth 時(shí)間: 2025-3-25 04:09 作者: Crumple 時(shí)間: 2025-3-25 08:08
Erika J. Ernst,P. David RogersIncludes supplementary material: 作者: 發(fā)電機(jī) 時(shí)間: 2025-3-25 14:20
Methods in Molecular Medicinehttp://image.papertrans.cn/a/image/158553.jpg作者: 初學(xué)者 時(shí)間: 2025-3-25 19:38 作者: 出處 時(shí)間: 2025-3-25 22:37 作者: semiskilled 時(shí)間: 2025-3-26 00:33 作者: Infuriate 時(shí)間: 2025-3-26 05:08 作者: perpetual 時(shí)間: 2025-3-26 11:56
1543-1894 evaluating both new and existing antifungal drugs, and for understanding the host response and immunotherapy of such agents. The protocols follow the successful Methods in Molecular Medicine? series format, each offering step-by-step laboratory instructions, an introduction outlining the principle b作者: 熒光 時(shí)間: 2025-3-26 14:11 作者: 古文字學(xué) 時(shí)間: 2025-3-26 20:08
Natural Products and Antifungal Drug Discoveryd drug prototypes, but also for probes of fungal biology. In this chapter, whole-cell screening methods targeted for natural products are illustrated, including general microplate-based screening, bioautography, and mode of action studies, including the use of genetically altered fungal strains now available commercially.作者: athlete’s-foot 時(shí)間: 2025-3-26 22:04 作者: medieval 時(shí)間: 2025-3-27 04:52
Jan Awrejcewicz,Vadim A. Kryskoner. Other models are selected to more closely mimic clinical infections. The following chapter will be limited to the description of a murine model of disseminated candidiasis in which in vitro results, pharmacokinetic properties, and a microbiological in vivo end point is used in the assessment of a wide variety of antifungal compounds.作者: arthroscopy 時(shí)間: 2025-3-27 07:56 作者: 雄偉 時(shí)間: 2025-3-27 10:30 作者: 罐里有戒指 時(shí)間: 2025-3-27 15:46
1.4.2 General features of the data,e .-specific repetitive DNA element .. Molecular typing with . permits highly reliable discrimination of unrelated strains to ascertain that serial isolates recovered from individual patients indeed represent the same . strain.作者: cortisol 時(shí)間: 2025-3-27 21:24 作者: Hectic 時(shí)間: 2025-3-27 22:48 作者: FLIT 時(shí)間: 2025-3-28 04:31
Mechanics of Elastic and Inelastic Solidsapter, the three most frequently used metholodologies are described: checkerboard, E-test, and time-kill curves. The description of each technique and intrepretaion of the results are addressed in detail.作者: 從屬 時(shí)間: 2025-3-28 10:18 作者: eustachian-tube 時(shí)間: 2025-3-28 12:37
Fingerprinting of , Isolatese .-specific repetitive DNA element .. Molecular typing with . permits highly reliable discrimination of unrelated strains to ascertain that serial isolates recovered from individual patients indeed represent the same . strain.作者: ICLE 時(shí)間: 2025-3-28 15:32 作者: 并置 時(shí)間: 2025-3-28 19:09 作者: 閑蕩 時(shí)間: 2025-3-28 23:32 作者: 龍蝦 時(shí)間: 2025-3-29 03:03 作者: 木質(zhì) 時(shí)間: 2025-3-29 08:21
Book 2005 drugs, and for understanding the host response and immunotherapy of such agents. The protocols follow the successful Methods in Molecular Medicine? series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary作者: 糾纏 時(shí)間: 2025-3-29 14:49 作者: Fortify 時(shí)間: 2025-3-29 19:14 作者: 敵手 時(shí)間: 2025-3-29 20:04 作者: MELON 時(shí)間: 2025-3-30 00:33
Evaluation of Immunotherapy in Invasive Candidiasise organism and subsequent extracellular release of oxidative and nonoxidative metabolites. An array of methods to evaluate phagocytosis of blastoconidia, oxidative burst, expression of cytokines and chemokines in response to . spp., as well as candidacidal activity are described in this chapter.作者: 領(lǐng)帶 時(shí)間: 2025-3-30 05:19 作者: GULLY 時(shí)間: 2025-3-30 11:24 作者: Oscillate 時(shí)間: 2025-3-30 13:56 作者: Jubilation 時(shí)間: 2025-3-30 18:09 作者: 偶像 時(shí)間: 2025-3-30 23:24 作者: 四海為家的人 時(shí)間: 2025-3-31 03:48 作者: 全能 時(shí)間: 2025-3-31 07:03
Fingerprinting of , Isolatesed example is the development of resistance to the widely used antifungal agent fluconazole in the yeast .. The availability of matched series of clinical isolates representing the same strain in which drug resistance developed over time has provided opportunities to detect cellular alterations that作者: 變態(tài) 時(shí)間: 2025-3-31 09:20 作者: 染色體 時(shí)間: 2025-3-31 14:51
Application of Deoxyribonucleic Acid Microarray Analysis to the Study of Azole Antifungal Resistance key genes involved in such clinical problems as the acquisition of high-level resistance to azole antifungal agents. Here, we outline in detail the methodologies utilized in our laboratory to culture clinical isolates of ., isolate ribonucleic acid from such cultures, synthesize labeled complimenta作者: 無(wú)效 時(shí)間: 2025-3-31 19:07 作者: Decimate 時(shí)間: 2025-3-31 22:48
Techniques for Antifungal Susceptibility Testing of ,ferent from their free-floating planktonic counterparts, including their recalcitrance to antimicrobial agents. Consequently, . biofilm-associated infections are difficult to treat and to fully eradicate with standard antifungal therapy. Here, we describe a simple, fast, inexpensive and highly repro