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標題: Titlebook: Antibody Usage in the Lab; Laura Caponi,Paola Migliorini Book 1999 Springer-Verlag Berlin Heidelberg 1999 Antibody.Antigen.ELISA.Immunoflu [打印本頁]

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Springer Lab Manualshttp://image.papertrans.cn/a/image/158480.jpg
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Planung und Betrieb hydraulischer Anlagen,ELISA (Enzyme-Linked ImmunoSorbent Assay) is a well-known and widely used laboratory technique that is able to measure the ligands present in small amounts in biological samples. ELISA combines a high degree of sensitivity and specificity with a detection system based on a colorimetric reaction; it can also provide quantitative results.
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Hans Jürgen Matthies,Karl Theodor Reniuscal studies. The availability of these tools has facilitated the localisation of various molecules in tissues and subcellular structures using immunocytochemical techniques. Immunocytochemistry is based on the ability of antibodies to bind a tissue antigen that can subsequently be revealed using direct or indirect techniques.
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Hans Jürgen Matthies,Karl Theodor Reniusty of these methods has increased because they are precise, sensitive and relatively inexpensive. In fact, antibodies have the ability to recognize their ligands with high specificity and can also be labeled fairly easily without significant loss of binding activity. Thanks to these properties, they
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,Elemente und Ger?te zur Energieübertragung,s, it is called Western blotting. Different techniques can be used to separate the proteins contained in a sample, such as SDS-PAGE (sodium dodecyl sulphate polyacrylamide electrophoresis, see Appendix 2), which is based on differences in the molecular weight of proteins, or isoelectrofocusing, whic
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https://doi.org/10.1007/978-3-8351-9240-9s procedure is based on the separation of the antibody-bound antigen from unbound antigens by means of reagents specific for the Fc portion of the immunoglobulins, usually protein A or protein G. Different preparations of unsolubilised protein A or G are commercially available. The matrix they are c
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Hans Jürgen Matthies,Karl Theodor Reniuscal studies. The availability of these tools has facilitated the localisation of various molecules in tissues and subcellular structures using immunocytochemical techniques. Immunocytochemistry is based on the ability of antibodies to bind a tissue antigen that can subsequently be revealed using dir
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978-3-662-03944-1Springer-Verlag Berlin Heidelberg 1999
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Immunohistochemistry,cal studies. The availability of these tools has facilitated the localisation of various molecules in tissues and subcellular structures using immunocytochemical techniques. Immunocytochemistry is based on the ability of antibodies to bind a tissue antigen that can subsequently be revealed using direct or indirect techniques.
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f each technique and sample protocols plus a troubleshooting guide. Attention is focused on the various aspects of the protocols described thus providing the reader with the maximum possible information on each technique.978-3-662-03944-1978-3-662-03942-7
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Introduction,ty of these methods has increased because they are precise, sensitive and relatively inexpensive. In fact, antibodies have the ability to recognize their ligands with high specificity and can also be labeled fairly easily without significant loss of binding activity. Thanks to these properties, they
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Antibody Specificity analyzed by Peptides synthesized on Cellulose Membranes,es have been developed for this purpose, including immunoblotting and ELISA (see Chapters 2 and 3), but one of the major limitations to the broader application of these techniques is the availability of synthetic peptides. In fact, while more and more laboratories now have their own automated peptid
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Book 1999he topics are discussed from a practical point of view, describing the theoretical basis of each technique and sample protocols plus a troubleshooting guide. Attention is focused on the various aspects of the protocols described thus providing the reader with the maximum possible information on each technique.
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https://doi.org/10.1007/978-3-8351-9240-9unoglobulins, usually protein A or protein G. Different preparations of unsolubilised protein A or G are commercially available. The matrix they are coupled to is usually sepharose, agarose or acrylic resins.
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,Elemente und Ger?te zur Energieübertragung,ght, being of different wavelengths, can be separated using optical filters. This is a sensitive technique since a positive signal is detected against a negative background. Furthermore, the ability to detect fluorescence simultaneously from two, three or four compounds fluorescing at different wavelengths allows multi-parametric analysis.
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Immunoblotting,lphate polyacrylamide electrophoresis, see Appendix 2), which is based on differences in the molecular weight of proteins, or isoelectrofocusing, which is based on differences in their isoelectric points. The separated antigens can then be transferred from the gel to a membrane for immunological characterisation.
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Antibody Specificity analyzed by Peptides synthesized on Cellulose Membranes,plication of these techniques is the availability of synthetic peptides. In fact, while more and more laboratories now have their own automated peptide synthesiser, the lack of a large number of synthetic peptides for epitope mapping experiments still constitutes a limiting step in many immunological laboratories.
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Hans Jürgen Matthies,Karl Theodor Reniusse techniques less hazardous to both users and the environment. Finally, the specificity and the high affinity or avidity of antibody binding make it an extremely sensitive technique for the detection of minute amounts of antigens.
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Introduction,se techniques less hazardous to both users and the environment. Finally, the specificity and the high affinity or avidity of antibody binding make it an extremely sensitive technique for the detection of minute amounts of antigens.
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