作者: 貝雷帽 時間: 2025-3-21 22:34 作者: V洗浴 時間: 2025-3-22 01:34 作者: Cleave 時間: 2025-3-22 07:38
https://doi.org/10.1007/978-3-662-25909-2anning on individual immobilized antigens (Ags) in order to isolate individual Ag-binding clones. This approach has been successfully applied to numerous purified soluble molecules, yielding high-affinity Abs (.). Selection against almost any soluble Ag is now theoretically feasible.作者: vertebrate 時間: 2025-3-22 10:24 作者: Coma704 時間: 2025-3-22 16:37 作者: 豐富 時間: 2025-3-22 20:42 作者: FLAG 時間: 2025-3-23 00:33 作者: coalition 時間: 2025-3-23 01:34
Selecting Antibodies to Cell-Surface Antigens Using Magnetic Sorting Techniques, is unknown (e.g., a putative stem cell or tumor-specific marker) or because the process of purification destroys its native conformation (e.g., in the case of some integral membrane proteins). In such experimental systems, methods that select phage-displayed Ig directly on intact cell surfaces are required.作者: pericardium 時間: 2025-3-23 07:37 作者: 強制性 時間: 2025-3-23 11:54 作者: 勤勞 時間: 2025-3-23 15:32
Rescue of a Broader Range of Antibody Specificities Using an Epitope-Masking Strategy,ries. The strategy uses antigen (Ag)-specific Fabs or single-chain Abs retrieved by an initial selection of the phage libraries to block the corresponding immunodominant epitope. Reselection of the phage library on the masked Ag refocuses the selection against other epitopes on the Ag (..).作者: GLUT 時間: 2025-3-23 19:55 作者: Hallmark 時間: 2025-3-23 22:15 作者: 砍伐 時間: 2025-3-24 03:08 作者: Aerophagia 時間: 2025-3-24 08:44
Karl Küpfmüller,Gerhard Kohn (o. Professor)resentative display libraries from the encoded proteins for screening. In many species, understanding of the basis to Ig formation is now sufficiently detailed for the application of these methods to MAb isolation.作者: 讓你明白 時間: 2025-3-24 14:07
,Der station?re elektrische Strom,) CA-Hb3, a 50-kDa protein that is recognized by monoclonal antibody (MAb), Hb3 (.), procedures were developed to drive the proliferation of specific B cells from the blood of patients, through exposure to Ag in vitro. This has enabled generation, through phage display, of recombinant human Abs against CA-Hb3.作者: 變白 時間: 2025-3-24 18:26
https://doi.org/10.1007/978-3-662-25911-5r detection of targets such as environmental pollutants or for monitoring the presence of drugs in clinical samples. There are also applications related to biological functions, e.g., Abs directed against signal molecules enhance the study of cell signaling pathways and have potential as candidate therapeutic agents.作者: 相反放置 時間: 2025-3-24 19:03
,Elektromagnetische Ausgleichsvorg?nge,not specific for the target of interest, but which bind to other proteins on the cell surface. Proximity (ProxiMol) selection is a method of selection that enriches the selected population for Abs that bind at or around sites on the cell surface of the target Ag and so reduce the need for labor-intensive screening processes.作者: Serenity 時間: 2025-3-25 01:23 作者: incision 時間: 2025-3-25 05:05 作者: MAG 時間: 2025-3-25 07:54 作者: Cuisine 時間: 2025-3-25 15:33
Proximity-Guided (ProxiMol) Antibody Selection,not specific for the target of interest, but which bind to other proteins on the cell surface. Proximity (ProxiMol) selection is a method of selection that enriches the selected population for Abs that bind at or around sites on the cell surface of the target Ag and so reduce the need for labor-intensive screening processes.作者: needle 時間: 2025-3-25 19:49
https://doi.org/10.1007/978-3-662-36777-3bulin M (IgM) taken from nonimmunized donors. By using this procedure, Abs were recovered prior to encounter with Ag and unscreened for tolerance by the immune system. Indeed, a na?ve library represents a good source of Abs against self, nonimmunogenic, and toxic Ags if the library is sufficiently large and diverse.作者: iodides 時間: 2025-3-25 20:52
Book 20021st editionrs will find similar clarity in the technical guidance offered by the book’s contributors. In meeting our objectives, we have tried to cover the broad scope of the technology and the key areas of library construction, scre- ing, antibody modification, and expression. Of course, the technology cont- 作者: Aboveboard 時間: 2025-3-26 03:31
1064-3745 bjectives, we have tried to cover the broad scope of the technology and the key areas of library construction, scre- ing, antibody modification, and expression. Of course, the technology cont- 978-0-89603-711-3978-1-59259-240-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 聾子 時間: 2025-3-26 05:16
,Elektromagnetische Ausgleichsvorg?nge, libraries (.,.). This chapter reviews the progress made in this rapidly developing field and discusses a broad range of applications, including the use of large phage Ab libraries to discover novel therapeutic targets and methods for selection of biologically active ligands. Finally, it addresses t作者: 項目 時間: 2025-3-26 09:16 作者: 功多汁水 時間: 2025-3-26 15:47
Systeme mit nichtlinearen Elementen,trials (.). Construction of such scFv libraries from naturally rearranged V genes in a phagemid vector ensures natural diversity in the length of the V. CDR3, a higher number of functional scFvs, and soluble scFv expression without the need for subcloning (.). Furthermore, phage-display technology a作者: expound 時間: 2025-3-26 17:08
,Der station?re elektrische Strom,f individual library members. Thus, a PCAL contains Abs specific for the target poly-Ag, and Abs that crossreact with other poly-Ags (e.g., normal cells). However, the Ag profile of the target poly-Ag differs qualitatively and quantitatively from the Ag profile of any other poly-Ag, enabling the PCA作者: floaters 時間: 2025-3-27 00:18 作者: 情感 時間: 2025-3-27 01:32
Systeme mit nichtlinearen Elementen,ins are nonfunctional (.,.) This problem is not important for a classical enzyme-linked immunosorbant assay (ELISA) mostly because a small fraction of proteins having a native conformation are still detectable. However, this phenomenon can be troublesome for phage Ab library selections because phage作者: 組成 時間: 2025-3-27 08:42 作者: Biguanides 時間: 2025-3-27 10:58
,Allgemeine Vorg?nge in linearen Systemen,s displayed as Fabs (.,.) or scFvs (.) on the surface of filamentous phages (for review, see .). Phage libraries have been constructed using a collection of cloned V. and/or V. fragments that have been assembled in vitro with random complementarity-determining region 3 (CDR3) (synthetic libraries) (作者: 帽子 時間: 2025-3-27 15:18
Overview of Antibody Phage-Display Technology and Its Applications, libraries (.,.). This chapter reviews the progress made in this rapidly developing field and discusses a broad range of applications, including the use of large phage Ab libraries to discover novel therapeutic targets and methods for selection of biologically active ligands. Finally, it addresses t作者: 一致性 時間: 2025-3-27 18:19 作者: subacute 時間: 2025-3-27 23:46 作者: 權(quán)宜之計 時間: 2025-3-28 05:10 作者: Offset 時間: 2025-3-28 07:23
8 The Recovery of Immunoglobulin Sequences from Single Human B Cells by Clonal Expansion, on the clonal expansion of single B cells in which cell-cell interactions (CD40-CD40L), as well as soluble factors, have been shown to be essential. One advantage beyond conventional hybridoma technology is that this method circumvents laborious plating and screening; the advantage compared to phag作者: gospel 時間: 2025-3-28 14:24
Selection of Antibodies Against Biotinylated Antigens,ins are nonfunctional (.,.) This problem is not important for a classical enzyme-linked immunosorbant assay (ELISA) mostly because a small fraction of proteins having a native conformation are still detectable. However, this phenomenon can be troublesome for phage Ab library selections because phage作者: 車床 時間: 2025-3-28 15:39 作者: Ige326 時間: 2025-3-28 20:42 作者: Expand 時間: 2025-3-29 01:15 作者: DEI 時間: 2025-3-29 05:20
Philippa M. O’Brien,Robert AitkenIncludes supplementary material: 作者: Stress 時間: 2025-3-29 09:40 作者: Charade 時間: 2025-3-29 13:08
,Elektromagnetische Ausgleichsvorg?nge, to mimic as closely as possible the desired in vivo activity of the new drug. New library methodologies offer many alternative routes that are at least as powerful as traditional approaches by combining the generation of billions of components with a fast screening or selection procedure to identif作者: 入會 時間: 2025-3-29 18:01
,Der station?re elektrische Strom, surface of the filamentous phage particle, have been widely used for the isolation of antibodies (Abs) with specificity for haptens, foreign antigens (Ags), and self Ags. Immune Fab libraries, in which lymphoid tissue from individuals who, perhaps because of disease, have mounted an immune response作者: 沉積物 時間: 2025-3-29 22:01 作者: BLA 時間: 2025-3-30 00:22
Karl Küpfmüller,Gerhard Kohn (o. Professor)unology. This can be explained by the lack of appropriate myeloma lines or transforming viruses for species outside this select group and the instability of heterohydridoma cell lines generated with, for example, murine myeloma lines (.). The advent of Ab phage-display technology offers a solution t作者: 無節(jié)奏 時間: 2025-3-30 04:03
https://doi.org/10.1007/978-3-662-36777-3ge panels of antigens (Ags). Na?ve libraries have been generated by tapping the natural primary (unselected) immune repertoire via cloning of Abs that recognize a variety of Ags (.,.). The rearranged V genes were amplified with the polymerase chain reaction (PCR) from B-cell mRNAs encoding immunoglo作者: Hyaluronic-Acid 時間: 2025-3-30 08:34 作者: 贊成你 時間: 2025-3-30 14:04 作者: Magnificent 時間: 2025-3-30 16:42
,Der station?re elektrische Strom,ers to virtually any antigen (.) (.). However, it is unlikely that the heavy (.) and light (.) chains of the Abs obtained from these libraries resemble original in vivo pairings. In certain autoimmune diseases and immunological processes, such as B-cell tolerance, these V. and V. combinations can be作者: Clumsy 時間: 2025-3-30 21:27
,Allgemeine Vorg?nge in linearen Systemen,he libraries that have been produced vary considerably, from small libraries of 10. up to large libraries>10.. Often an Ab with the desired specificity exists at low frequencies in the recombinant Ab repertoire. It is therefore necessary to have an effective technique for the enrichment and identifi作者: 松雞 時間: 2025-3-31 02:01
Systeme mit nichtlinearen Elementen,g., Petri dishes, microtiter plate wells, and immunotubes). This straightforward method is easy to perform and has been shown to be successful for a diverse set of Ags (for review, ..). However, phage Ab selections on some proteins and especially on peptides are not always successful, which is often作者: commodity 時間: 2025-3-31 07:58 作者: 混合 時間: 2025-3-31 10:10
https://doi.org/10.1007/978-3-662-25911-5al antibodies (MAbs) against a plethora of viral antigens (Ag) (.-.). However, sometimes the isolation of a given Ab can be particularly difficult because of the predominance of some epitopes, and, in the case of impure Ags, because the protein or compound of interest is present in a low amount. Man作者: anarchist 時間: 2025-3-31 16:34
,Ausgleichsvorg?nge in linearen Systemen,al antibodies (MAbs) with desired specificity (.,.). However, following selection of such libraries, certain epitope specificities may dominate the cloned Abs because, for example, the epitope is immunodominant or because Abs to that epitope have a selective advantage in the panning process arising 作者: ULCER 時間: 2025-3-31 21:29 作者: 通知 時間: 2025-4-1 00:27
https://doi.org/10.1007/978-3-662-25912-2 as bone marrow, lymph node, or peripheral blood lymphocytes from patients with various diseases, or from healthy individuals (.,.). Many human MAb Fabs or scFvs specific for viral pathogens, self antigens (Ags), or nonself Ags have been isolated by phage display system. This technology is expected 作者: ABIDE 時間: 2025-4-1 02:29 作者: construct 時間: 2025-4-1 09:47 作者: 歪曲道理 時間: 2025-4-1 14:03 作者: chemical-peel 時間: 2025-4-1 17:36
,Allgemeine Vorg?nge in linearen Systemen,pecific immunotherapy of malignant diseases. For example, hybridoma technology has been successfully used to identify TAA recognized by mouse monoclonal Abs (for review, ..). The SEREX methodology, i.e., serological analysis of recombinant cDNA expression libraries prepared from malignant cells, has作者: 使入迷 時間: 2025-4-1 19:10
