標題: Titlebook: Antibody Methods and Protocols; Gabriele Proetzel,Hilmar Ebersbach Book 2012 Springer Science+Business Media, LLC 2012 Antibodies.Antibody [打印本頁] 作者: 氣泡 時間: 2025-3-21 16:08
書目名稱Antibody Methods and Protocols影響因子(影響力)
書目名稱Antibody Methods and Protocols影響因子(影響力)學科排名
書目名稱Antibody Methods and Protocols網(wǎng)絡公開度
書目名稱Antibody Methods and Protocols網(wǎng)絡公開度學科排名
書目名稱Antibody Methods and Protocols被引頻次
書目名稱Antibody Methods and Protocols被引頻次學科排名
書目名稱Antibody Methods and Protocols年度引用
書目名稱Antibody Methods and Protocols年度引用學科排名
書目名稱Antibody Methods and Protocols讀者反饋
書目名稱Antibody Methods and Protocols讀者反饋學科排名
作者: 無底 時間: 2025-3-21 21:29 作者: connoisseur 時間: 2025-3-22 01:56 作者: Desert 時間: 2025-3-22 05:25 作者: 佛刊 時間: 2025-3-22 11:34
Methods to Engineer and Identify IgG1 Variants with Improved FcRn Binding or Effector Function,nts through convenience in dosing or increased efficacy. Here we describe protocols for generating Fc-engineered IgG. antibodies and assays to measure Fc receptor binding, antibody dependent cellular cytotoxicity activity, and complement dependent cytotoxicity activity to identify variants with improved FcRn binding or effector function.作者: BIPED 時間: 2025-3-22 16:39
1064-3745 ps on troubleshooting and avoiding known experimental pitfal.The rapidly growing field of antibody research is the result of many advancing technologies allowing current developments to take advantage of molecular engineering to create tailor-made antibodies.? .Antibody Methods and Protocols. attemp作者: Modicum 時間: 2025-3-22 18:51 作者: connoisseur 時間: 2025-3-22 23:40 作者: Canyon 時間: 2025-3-23 04:58
,Sichtbarmachung von Str?mungen, of the development of best in class monoclonal antibodies. Here, a robust surface plasmon resonance-based protocol is presented, which describes a sensitive temperature-dependent kinetic measurement and evaluation method.作者: 品嘗你的人 時間: 2025-3-23 08:23
,Einflu? der Reibung bei umstr?mten K?rpern,herapeutic antibody may thus be modulated according to the class of its constant domains. Depending on the immunoglobulin class, different functional assays will be used in order to evaluate the functional activity of a monoclonal antibody.作者: Gastric 時間: 2025-3-23 12:10 作者: 沉思的魚 時間: 2025-3-23 17:00 作者: 浮雕 時間: 2025-3-23 20:19
Temperature-Dependent Antibody Kinetics as a Tool in Antibody Lead Selection, of the development of best in class monoclonal antibodies. Here, a robust surface plasmon resonance-based protocol is presented, which describes a sensitive temperature-dependent kinetic measurement and evaluation method.作者: Cpr951 時間: 2025-3-24 00:14
Class-Specific Effector Functions of Therapeutic Antibodies,herapeutic antibody may thus be modulated according to the class of its constant domains. Depending on the immunoglobulin class, different functional assays will be used in order to evaluate the functional activity of a monoclonal antibody.作者: GEON 時間: 2025-3-24 04:48 作者: infinite 時間: 2025-3-24 09:54
Einführung in die technische Str?mungslehre for hybridoma generation are discussed and detailed in the following sections: cell fusion for hybridoma generation, antibody screening and characterization, hybridoma subcloning and mAb isotyping, as well as production of mAbs from hybridoma cells.作者: 抗原 時間: 2025-3-24 12:37
Einführung in die technische Str?mungslehreameters, which comprehensively describe the antibody’s kinetic rate profile and its valence mode. The method enables the scientist to rank and finally select rare and outstanding antibodies according to their kinetic signatures.作者: 600 時間: 2025-3-24 18:23 作者: MAIM 時間: 2025-3-24 21:23
Kinetic Screening in the Antibody Development Process,ameters, which comprehensively describe the antibody’s kinetic rate profile and its valence mode. The method enables the scientist to rank and finally select rare and outstanding antibodies according to their kinetic signatures.作者: 哥哥噴涌而出 時間: 2025-3-25 01:23
Book 2012lar engineering to create tailor-made antibodies.? .Antibody Methods and Protocols. attempts to provide insight into the generation of antibodies using in vitro and in vivo approaches, as well as technical aspects for screening, analysis, and modification of antibodies and antibody fragments.? The d作者: Infusion 時間: 2025-3-25 03:40
1064-3745 ry protocols, and tips on troubleshooting and avoiding known pitfalls..?.Authoritative and easy to use, .Antibody Methods and Protocols. provides a broad and useful background to support ongoing efforts by novi978-1-4939-5930-3978-1-61779-931-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: ciliary-body 時間: 2025-3-25 10:51 作者: Irascible 時間: 2025-3-25 13:24
Antigen Presentation for the Generation of Binding Molecules,nding molecules. Moreover, it is also necessary to consider the expression host and any posttranslational modifications of target proteins. The increasing demand to target more complex antigens, for instance, receptors and ion channels, is leading to the development of alternative procedures to pres作者: nonplus 時間: 2025-3-25 16:43 作者: incredulity 時間: 2025-3-25 22:06 作者: Mri485 時間: 2025-3-26 03:24
PEGylation of Antibody Fragments for Half-Life Extension,e hinge cysteine or to C-terminal cysteines involved in interchain disulfide linkage of the heavy and light chain. Hence, protocols for mono-PEGylation of Fab via free cysteine in the hinge region and di-PEGylation of Fab via interchain disulfide bridge are provided in this chapter.作者: 比目魚 時間: 2025-3-26 08:20
Bispecific Antibody Derivatives Based on Full-Length IgG Formats,tibody formats that can be prepared in high yields and high homogeneity using conventional expression and purification techniques. Especially, recent development of IgG-fusions with disulfide-stabilized Fv fragments and of CrossMab-technologies facilitates the generation of bispecific antibodies wit作者: malapropism 時間: 2025-3-26 10:20 作者: lanugo 時間: 2025-3-26 12:59
,Sichtbarmachung von Str?mungen,nding molecules. Moreover, it is also necessary to consider the expression host and any posttranslational modifications of target proteins. The increasing demand to target more complex antigens, for instance, receptors and ion channels, is leading to the development of alternative procedures to pres作者: 山間窄路 時間: 2025-3-26 17:18
Einführung in die technische Str?mungslehre functions. In some instances, where there is a need for in vivo functional assays of a single antibody with a known specificity, it might be of interest to transiently express that gene in mice by in vivo gene transfer. This approach allows a rapid functional assay. More commonly, mice are used to 作者: CAMEO 時間: 2025-3-26 21:25
https://doi.org/10.1007/978-3-642-91495-9cribes two different mass spectrometry based methods used for analyses of the carbohydrate moieties attached to the Fc-part of human IgG1. In the first approach, the glycans are released from the antibody by endoglycosidase (Peptide N Glycosidase F) digestion and monitored by matrix-assisted laser d作者: 高射炮 時間: 2025-3-27 04:17
Einführung in die technische Str?mungslehree hinge cysteine or to C-terminal cysteines involved in interchain disulfide linkage of the heavy and light chain. Hence, protocols for mono-PEGylation of Fab via free cysteine in the hinge region and di-PEGylation of Fab via interchain disulfide bridge are provided in this chapter.作者: 漂亮才會豪華 時間: 2025-3-27 06:49
Einführung in die technische Str?mungslehretibody formats that can be prepared in high yields and high homogeneity using conventional expression and purification techniques. Especially, recent development of IgG-fusions with disulfide-stabilized Fv fragments and of CrossMab-technologies facilitates the generation of bispecific antibodies wit作者: 偏狂癥 時間: 2025-3-27 12:11
https://doi.org/10.1007/978-3-642-91496-6y of IgG–citrine derivatives. Because IgG–citrine fusions are stable and retain biophysical properties of IgGs, they can be expressed and purified in the same manner as regular antibodies. IgG–citrine fusions not only retain the binding properties (affinity and specificity) of antibodies but also co作者: ABIDE 時間: 2025-3-27 17:29
Antigen Presentation for the Generation of Binding Molecules,olds from in vivo and in vitro sources. These methods encompass robust techniques including immunization and hybridoma technology or phage display and also more laborious and novel approaches including ribosome display or B-cell immortalization. All methodologies are dependent upon proper antigen pr作者: gangrene 時間: 2025-3-27 19:19 作者: 謙虛的人 時間: 2025-3-28 00:05
Phage Display,ntages over traditional ways of antibody generation such as mouse hybridoma techniques. While there are various possibilities to conduct phage display, selection of antibodies via solution panning is an elegant way to circumvent conformation changes of antigen, which may arise when performing pannin作者: mediocrity 時間: 2025-3-28 02:13 作者: promote 時間: 2025-3-28 06:37
Ribosome Display,ins. This technology exploits cell-free translation to achieve coupling of phenotype and genotype by the production of stabilized ribosome complexes, whereby translated protein and their cognate mRNA remain attached to the ribosome. The . S30 extract for in vitro display of an mRNA library has prove作者: Legend 時間: 2025-3-28 13:19 作者: 鳴叫 時間: 2025-3-28 14:46
The Application of Transgenic Mice for Therapeutic Antibody Discovery,ion (FDA). Since then, a further seven such antibodies have been approved. In this chapter, we discuss how transgenic mice technologies can provide a powerful platform for creating human therapeutic antibodies.作者: calorie 時間: 2025-3-28 22:15
Production of Human or Humanized Antibodies in Mice,fined immunization protocols. Such an approach allows optimal in vivo affinity maturation of the humoral response. In addition, high-affinity antibodies arising in this context can readily be further characterized and produced as monoclonals after immortalizing and selecting specific antibody-produc作者: 和諧 時間: 2025-3-29 02:12 作者: FLING 時間: 2025-3-29 04:41 作者: 枯萎將要 時間: 2025-3-29 09:32 作者: 冷漠 時間: 2025-3-29 14:28 作者: 錯 時間: 2025-3-29 17:59
Cloning, Expression, and Purification of Monoclonal Antibodies in scFv-Fc Format,v-Fc expression cassette into a mammalian expression vector followed by transient transfection of mammalian cells and purification by protein A affinity chromatography. The protocol is intended for applications in basic and preclinical research that require rapid access to milligram amounts of prote作者: 一起平行 時間: 2025-3-29 19:46
PEGylation of Antibody Fragments for Half-Life Extension,antages, including higher mobility and tissue penetration, ability to bind antigen monovalently and lack of fragment crystallizable (Fc) region-mediated functions such as antibody-dependent cell mediated cytotoxicity (ADCC) or complement-dependent cytotoxicity (CDC). The main drawback for the use of作者: cluster 時間: 2025-3-30 01:02 作者: 解開 時間: 2025-3-30 06:28 作者: 過多 時間: 2025-3-30 08:19 作者: Adulate 時間: 2025-3-30 12:45 作者: 卡死偷電 時間: 2025-3-30 20:25
Antibody Methods and Protocols978-1-61779-931-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 不要嚴酷 時間: 2025-3-30 23:10
,Sichtbarmachung von Str?mungen,s for their subsequent selection by phage display. Although it can be applied to the mining of both human na?ve and immune antibody repertoires, the procedure is primarily intended for the generation of fully human monoclonal antibodies from patients with endogenous antibody responses of interest and limited availability of clinical specimens.作者: 胎兒 時間: 2025-3-31 02:39
Einführung in die technische Str?mungslehreion (FDA). Since then, a further seven such antibodies have been approved. In this chapter, we discuss how transgenic mice technologies can provide a powerful platform for creating human therapeutic antibodies.作者: 安心地散步 時間: 2025-3-31 05:42 作者: 臭名昭著 時間: 2025-3-31 09:12 作者: certain 時間: 2025-3-31 17:01 作者: PTCA635 時間: 2025-3-31 20:15 作者: 思鄉(xiāng)病 時間: 2025-3-31 22:19
Selection of Human Fab Libraries by Phage Display,This protocol describes the selection of human antibody libraries in Fab format by phage display. It includes panning against immobilized antigens, biotinylated antigens in solution, and cell surface antigens.作者: 性學院 時間: 2025-4-1 03:53
Generation of Human Fab Libraries for Phage Display,s for their subsequent selection by phage display. Although it can be applied to the mining of both human na?ve and immune antibody repertoires, the procedure is primarily intended for the generation of fully human monoclonal antibodies from patients with endogenous antibody responses of interest and limited availability of clinical specimens.作者: 極端的正確性 時間: 2025-4-1 07:52
The Application of Transgenic Mice for Therapeutic Antibody Discovery,ion (FDA). Since then, a further seven such antibodies have been approved. In this chapter, we discuss how transgenic mice technologies can provide a powerful platform for creating human therapeutic antibodies.作者: 同音 時間: 2025-4-1 11:57 作者: Occupation 時間: 2025-4-1 18:03 作者: 表示向前 時間: 2025-4-1 19:45
