標(biāo)題: Titlebook: Antibody Engineering; Methods and Protocol Damien Nevoltris,Patrick Chames Book 2018Latest edition Springer Science+Business Media, LLC, pa [打印本頁] 作者: 懇求 時間: 2025-3-21 19:47
書目名稱Antibody Engineering影響因子(影響力)
書目名稱Antibody Engineering影響因子(影響力)學(xué)科排名
書目名稱Antibody Engineering網(wǎng)絡(luò)公開度
書目名稱Antibody Engineering網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Antibody Engineering被引頻次
書目名稱Antibody Engineering被引頻次學(xué)科排名
書目名稱Antibody Engineering年度引用
書目名稱Antibody Engineering年度引用學(xué)科排名
書目名稱Antibody Engineering讀者反饋
書目名稱Antibody Engineering讀者反饋學(xué)科排名
作者: 國家明智 時間: 2025-3-21 23:23
https://doi.org/10.1007/978-3-662-02149-1gen encounter by somatically mutating their genome sequences. The end result is a series of antibodies that display higher affinities and specificities to specific antigens. This process is called affinity maturation. Recent improvements in computer hardware and modeling algorithms now enable the ra作者: 憤慨點吧 時間: 2025-3-22 01:35 作者: Ordeal 時間: 2025-3-22 05:56 作者: 用不完 時間: 2025-3-22 11:05
Einführung in die organische Chemiedescribe a simple protocol to make large and diverse libraries based on a single or a limited number of frameworks. The approach is flexible enough to be used with any antibody format, either single-chain (scFv, VHH) or multi-chain (Fv, Fab, (Fab′)2), and to target in a single step the six complemen作者: Modicum 時間: 2025-3-22 14:10 作者: 序曲 時間: 2025-3-22 18:59 作者: 陳腐思想 時間: 2025-3-22 21:37
Einführung in die organische Chemie a tedious multistep process that involves the generation of heavy chain as well as light chain display plasmids in different haploid yeast strains followed by yeast mating. Here, we present a focused one-step Golden Gate cloning approach for the generation of yeast surface display Fab libraries tha作者: 陶醉 時間: 2025-3-23 04:13
Stickstoffhaltige Verbindungen,tic tool development. In many case, the protein of interest, or a relevant portion of this protein such as its extracellular domain, is available as purified protein. In such cases, phage display on purified antigen is an easy and fast way to select antibody fragment able to efficiently bind this an作者: 歌劇等 時間: 2025-3-23 07:19
Einführung in die organische Chemiesystem. Approximately 38% of the entire genome codes for plasma membrane proteins; however the discovery and development of antibody binders to such targets are a technical challenge. Methods to raise binders against such targets by cloning and expressing soluble extracellular regions have been met 作者: 線 時間: 2025-3-23 12:00
Stickstoffhaltige Verbindungen,lex structures. Such structures have been shown to form in cells in a dynamic manner. Monoclonal antibodies against such structures represent key tools to study the biological functions of these structures. Here we provide protocols for the generation of antibody fragments against structured DNA usi作者: chalice 時間: 2025-3-23 15:22
Sauerstoffhaltige Verbindungen (II),quired the development of new platforms of discovery. Yeast display is one of the main methods of in vitro display technology with phage display. Yeast display has been chosen by numerous groups to refine both affinity and specificity of antibodies because it enables fine discrimination between muta作者: 持久 時間: 2025-3-23 19:09 作者: Interlocking 時間: 2025-3-23 23:17
Einführung in die organische Chemieolecules, in vitro selection using cell-free display technologies such as cDNA display is useful and powerful because of their huge libraries and robust stability. We provide technical details for in vitro selection of single-domain antibodies using cDNA display.作者: Thyroiditis 時間: 2025-3-24 03:15 作者: FECT 時間: 2025-3-24 09:25
Damien Nevoltris,Patrick ChamesIncludes cutting-edge techniques for the study of antibody engineering.Provides step-by-step detail essential for reproducible results.Contains updated key implementation advice from the experts作者: prostatitis 時間: 2025-3-24 10:53
Methods in Molecular Biologyhttp://image.papertrans.cn/a/image/158471.jpg作者: antiquated 時間: 2025-3-24 16:39 作者: Interregnum 時間: 2025-3-24 22:09 作者: 襲擊 時間: 2025-3-24 23:10 作者: 我說不重要 時間: 2025-3-25 05:44
Einführung in die organische Chemiegen, as well as methods for the amplification and sequencing of B cell receptor (BCR) heavy and light chain genes. Detailed protocols are provided for transient mammalian expression in a hexahistidine-tagged Fab format, purification by immobilized metal affinity chromatography (IMAC), and affinity determination by BioLayer interferometry (BLI).作者: Obsessed 時間: 2025-3-25 11:28 作者: IRATE 時間: 2025-3-25 13:31
978-1-4939-9353-6Springer Science+Business Media, LLC, part of Springer Nature 2018作者: SOBER 時間: 2025-3-25 17:19
Selection of Antibody Fragments Against Structured DNA by Phage Displaylex structures. Such structures have been shown to form in cells in a dynamic manner. Monoclonal antibodies against such structures represent key tools to study the biological functions of these structures. Here we provide protocols for the generation of antibody fragments against structured DNA using phage display selections.作者: 疏遠天際 時間: 2025-3-25 22:04 作者: 小樣他閑聊 時間: 2025-3-26 01:26 作者: paroxysm 時間: 2025-3-26 05:25 作者: MAUVE 時間: 2025-3-26 10:18
Antibody Design and Humanization via In Silico Modelingzation failures. Recently, there has been advent of homology modelor structure-guided antibody humanization. Instead of humanization based on linear sequence, this approach takes into account the tertiary structure and fold of the mouse antibody. A mouse homology model of the fragment variable?is cr作者: 泥沼 時間: 2025-3-26 14:03
Use of IMGT? Databases and Tools for Antibody Engineering and Humanizationerence for immunogenetics and immunoinformatics, and its standards are particularly useful for antibody engineering and humanization. IMGT. databases for antibody nucleotide sequences and genes include IMGT/LIGM-DB and IMGT/GENE-DB, respectively, and nucleotide sequence analysis is performed by the 作者: Erythropoietin 時間: 2025-3-26 18:56 作者: Laconic 時間: 2025-3-26 23:59 作者: enfeeble 時間: 2025-3-27 01:50 作者: capsaicin 時間: 2025-3-27 07:41 作者: Endoscope 時間: 2025-3-27 10:56 作者: FLIP 時間: 2025-3-27 17:41
Sauerstoffhaltige Verbindungen (I),specificity but also for stability and catalytic activity. High-affinity binders with new target specificity can be obtained from highly diverse libraries in only a few selection rounds. In this protocol, the selection from the library and the process of affinity maturation and off-rate selection ar作者: indemnify 時間: 2025-3-27 20:01
Chemische Grundgesetze und Grundbegriffe, This protocol is based on many different sets of oligonucleotide primers and multistep electroporation and ligation reactions..This advanced method can be adopted by any molecular biology laboratory to generate a na?ve library for use in isolating single-chain fragment variables against specific targets.作者: 夸張 時間: 2025-3-28 01:15 作者: Morbid 時間: 2025-3-28 05:51
1064-3745 ins updated key implementation advice from the expertsThis detailed new edition provides complete and easy access to a variety of antibody engineering techniques.? The volume explores topics such as the generation of native, synthetic, or immune antibody libraries, the selection of lead candidates v作者: 充滿人 時間: 2025-3-28 08:59
Einführung in die organische Chemie be used with any antibody format, either single-chain (scFv, VHH) or multi-chain (Fv, Fab, (Fab′)2), and to target in a single step the six complementarity-determining regions—or any other part—of the antibody molecule. Using this protocol, libraries larger than 10. can be constructed in a single week.作者: 來自于 時間: 2025-3-28 12:59
Construction of Synthetic Antibody Libraries be used with any antibody format, either single-chain (scFv, VHH) or multi-chain (Fv, Fab, (Fab′)2), and to target in a single step the six complementarity-determining regions—or any other part—of the antibody molecule. Using this protocol, libraries larger than 10. can be constructed in a single week.作者: Etymology 時間: 2025-3-28 17:26 作者: Synthesize 時間: 2025-3-28 22:05
https://doi.org/10.1007/978-3-662-02149-1y describe computational methods for antibody affinity maturation, focusing on methods for sampling antibody conformations and for scoring designed antibody variants. We also discuss lessons learned from the successful computer-aided design of antibodies.作者: insecticide 時間: 2025-3-29 02:15 作者: 負擔(dān) 時間: 2025-3-29 06:44 作者: ETHER 時間: 2025-3-29 08:37 作者: 引起痛苦 時間: 2025-3-29 12:46 作者: stroke 時間: 2025-3-29 19:23
Display Technologies for Generation of Ig Single Variable Domains in vivo matured antigen-specific clones with comprehensive functionalities. In this chapter, we describe a detailed protocol for construction of VHH immune libraries and phage display selection against antigens in their native conformation.作者: 鉆孔 時間: 2025-3-29 20:19
Phage Display and Selections on Purified Antigensowing protocols describe the selection of phage antibody on purified antigen adsorbed on plastic, i.e., panning, or a selection in solution, using a biotinylated antigen as well as the corresponding screening produces, and give hints on the advantage and drawbacks of each approach.作者: 不能和解 時間: 2025-3-30 00:26
Selection of Antibody Fragments by Yeast Display repertoire more fully than using phage. This chapter gives an updated overview of the available systems of yeast display platforms and libraries, followed up by technical descriptions of selection methods of antibody fragments by yeast display.作者: Favorable 時間: 2025-3-30 07:57
Construction of Human Na?ve Antibody Gene Libraries This protocol is based on many different sets of oligonucleotide primers and multistep electroporation and ligation reactions..This advanced method can be adopted by any molecular biology laboratory to generate a na?ve library for use in isolating single-chain fragment variables against specific targets.作者: 大吃大喝 時間: 2025-3-30 11:54 作者: cushion 時間: 2025-3-30 15:39
Book 2018Latest editiondily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.?.Authoritative and up-to-date, .Antibody Engineering: Methods and Protocols, Third Edition. presents the reader with an extensive toolbox to create the powerful molecules of tomorrow..作者: 暴露他抗議 時間: 2025-3-30 19:00 作者: 否決 時間: 2025-3-31 00:31 作者: 爭吵加 時間: 2025-3-31 04:40 作者: 主動 時間: 2025-3-31 07:32
Antibody Affinity Maturation by Computational Designgen encounter by somatically mutating their genome sequences. The end result is a series of antibodies that display higher affinities and specificities to specific antigens. This process is called affinity maturation. Recent improvements in computer hardware and modeling algorithms now enable the ra作者: 不容置疑 時間: 2025-3-31 10:02
Use of IMGT? Databases and Tools for Antibody Engineering and Humanizationnage the huge diversity of the antigen receptors, immunoglobulins (IG) or antibodies, and T cell receptors (TR). The founding of IMGT. marked the advent of immunoinformatics, which emerged at the interface between immunogenetics and bioinformatics. Standardized sequence and structure analysis of ant作者: 徹底明白 時間: 2025-3-31 13:27
Construction of Human Na?ve Antibody Gene Librariesens. Therefore, optimizing the construction of a large library is essential for the selection of high-affinity reactive fragments. Here, we report a highly efficient method for the construction of a human na?ve antibody gene library for the selection of antibodies as single-chain variable fragments.作者: 樹木中 時間: 2025-3-31 19:41 作者: Fabric 時間: 2025-3-31 22:17
Construction of Histidine-Enriched Shark IgNAR Variable Domain Antibody Libraries for the Isolation n binding in case of IgNAR antibodies is mediated by a single variable domain (vNAR). Due to their inherent beneficial biophysical properties, such as small size and high thermal stability combined with a high specificity and affinity to their target antigens, vNAR domains emerged as promising tools
