標(biāo)題: Titlebook: Antibody Engineering; Roland Kontermann,Stefan Dübel Book 20011st edition Springer-Verlag Berlin Heidelberg 2001 Amino acid.Antigen.Cell p [打印本頁(yè)] 作者: 新石器時(shí)代 時(shí)間: 2025-3-21 16:46
書目名稱Antibody Engineering影響因子(影響力)
書目名稱Antibody Engineering影響因子(影響力)學(xué)科排名
書目名稱Antibody Engineering網(wǎng)絡(luò)公開度
書目名稱Antibody Engineering網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Antibody Engineering被引頻次
書目名稱Antibody Engineering被引頻次學(xué)科排名
書目名稱Antibody Engineering年度引用
書目名稱Antibody Engineering年度引用學(xué)科排名
書目名稱Antibody Engineering讀者反饋
書目名稱Antibody Engineering讀者反饋學(xué)科排名
作者: MILL 時(shí)間: 2025-3-21 20:41
Construction of scFv Fragments from Hybridoma or Spleen Cells by PCR Assembly et al. 2000), very many hybridomas have been generated, and are continuously being made, which produce monoclonal antibodies with very interesting properties. Even when naive and synthetic libraries may be a source of antibodies, often the immune response of an experimental animal may be of interes作者: 洞穴 時(shí)間: 2025-3-22 03:44
Construction of scFv from Hybridoma by Two-Step Cloning available from hybridoma cell lines. Here, a method is presented to obtain the genetic information for the antigen binding part of the antibody from hybridoma cells, and to assemble it into a functional bacterially expressed fusion protein (scFv fragment). To achieve this, vectors have been constru作者: fidelity 時(shí)間: 2025-3-22 07:18 作者: Dawdle 時(shí)間: 2025-3-22 11:27 作者: 痛打 時(shí)間: 2025-3-22 14:13
Generation of Naive Human Antibody Librariesdes and folded proteins could be displayed on the surface of filamentous bacteriophage (Smith, 1985; Bass et al., 1990). Since the generation of the first human antibodies by phage display (Winter et al., 1994), the technology has developed to the point where large scFv repertoires have been created作者: Exaggerate 時(shí)間: 2025-3-22 17:39
Generation of Antibody Gene Libraries from Seropositive Human Donors however, antibodies to threatening antigens cannot be produced, as in the case of most cancer cells, or they appear too late to control the antigen, as e. g. in the case of intoxication. In this situation, therapeutic antibodies can be applied to help our own immune system. Further, therapeutic ant作者: 乞討 時(shí)間: 2025-3-22 22:34
Generation of Rabbit Immune Librariesat proteins provides an alternative strategy for the rapid generation of monoclonal antigen-binding proteins. Various antibody-displaying phage libraries have been described, which are based on the B cell repertoire of rearranged immunoglobulin genes from spleen and bone marrow of previously immuniz作者: 積習(xí)已深 時(shí)間: 2025-3-23 02:50 作者: monologue 時(shí)間: 2025-3-23 09:09 作者: headlong 時(shí)間: 2025-3-23 13:38
Proximity (ProxiMol) and Step-Back Selectionsnically complex and this results in difficulties in specifically selecting antibodies which bind to a given cell surface molecule without selecting for a host of unwanted specificities in parallel. The desired antigen specificity can in many cases be obtained, but only after an intensive screening p作者: 違抗 時(shí)間: 2025-3-23 15:38 作者: 全能 時(shí)間: 2025-3-23 21:46
Selection of Human Antibody Fragments by Anti-Idiotypic Interaction compared to the background phages or the antigen is not available in a suitable form, resulting in nonspecific adherence of the phages. For this reason researchers used anti-idiotypic antibodies for the panning procedure. These were generated by immunization of rabbits with human IgG Fab (idiotype)作者: SPASM 時(shí)間: 2025-3-23 23:01 作者: 金桌活畫面 時(shí)間: 2025-3-24 06:07 作者: objection 時(shí)間: 2025-3-24 10:14 作者: erythema 時(shí)間: 2025-3-24 11:11
Recombinant Antibodiesle, an immense number of different molecules, bearing different specificities, is required. This diversity is generated by somatic recombination and hypermutagenesis of a set of variant genes. The genetic information for this repertoire of different antibodies is stored in the B-cell pool of our lymphatic system.作者: extinct 時(shí)間: 2025-3-24 18:33 作者: 平淡而無(wú)味 時(shí)間: 2025-3-24 19:52 作者: 遺產(chǎn) 時(shí)間: 2025-3-24 23:23
https://doi.org/10.1007/978-3-662-04605-0Amino acid; Antigen; Cell preparation; Expression; Immunologie; Polymerasekettenreaktion; Zellpreparatione作者: duplicate 時(shí)間: 2025-3-25 04:26 作者: dendrites 時(shí)間: 2025-3-25 08:57
,Opfer und ihre Rechte (§§ 65–70),le, an immense number of different molecules, bearing different specificities, is required. This diversity is generated by somatic recombination and hypermutagenesis of a set of variant genes. The genetic information for this repertoire of different antibodies is stored in the B-cell pool of our lym作者: 相信 時(shí)間: 2025-3-25 13:47 作者: 方舟 時(shí)間: 2025-3-25 19:27 作者: 有惡臭 時(shí)間: 2025-3-25 20:19
,Opfer und ihre Rechte (§§ 65–70),wever, there are a number of potential pitfalls in using V region PCR. Mutations within the 5′ or 3′ ends of the V genes may inhibit primer annealing and so prevent amplification. Another problem is the presence of other V genes within the hybridoma which are preferentially amplified. These arise fo作者: 親屬 時(shí)間: 2025-3-26 02:16
Einführung in die neue Strafprozessordnungs displayed on the surface of filamentous phage. Phagemid vectors are used for placing randomly paired light (L) and heavy (H) chain coding regions under transcriptional control of . The L (or H) chain coding region is fused in-frame with the phage gene, .g., coding for a truncated version of the ph作者: obstinate 時(shí)間: 2025-3-26 06:37 作者: Ringworm 時(shí)間: 2025-3-26 12:30 作者: FLIT 時(shí)間: 2025-3-26 13:39 作者: 清楚說(shuō)話 時(shí)間: 2025-3-26 18:50
,Ausschlie?ung und Befangenheit (§§ 43–47), from phage display libraries (Marks et al., 1991; Harrison et al., 1996; for review see Winter et al., 1994). The protocol shown here describes selections from a phagemid library (Vaughan et al., 1996) (see also Chapter 6) based on phagemid vectors such as pCANTAB6 (McCafferty et al., 1994). In the作者: 新奇 時(shí)間: 2025-3-27 00:05
Einführung in die neue Strafprozessordnunges, microtiter plate well, immunotubes, Chapter 9). This straightforward method is easy to perform and has been shown to be very successful for a diverse set of antigens (for review see Winter et al. 1994). However, phage-antibody selections on some proteins and especially on peptides are not always作者: aptitude 時(shí)間: 2025-3-27 04:55 作者: Polydipsia 時(shí)間: 2025-3-27 07:05 作者: 褲子 時(shí)間: 2025-3-27 12:37 作者: preservative 時(shí)間: 2025-3-27 14:48
,Opfer und ihre Rechte (§§ 65–70),in organisms [1]. The efficacy of the ectopic antibody expression has been already demonstrated in several cases and its use has potential applications to disease therapy and in functional genomic studies.作者: 斜 時(shí)間: 2025-3-27 18:39 作者: 歌劇等 時(shí)間: 2025-3-27 23:00 作者: Nefarious 時(shí)間: 2025-3-28 02:34
,Opfer und ihre Rechte (§§ 65–70),in organisms [1]. The efficacy of the ectopic antibody expression has been already demonstrated in several cases and its use has potential applications to disease therapy and in functional genomic studies.作者: 自然環(huán)境 時(shí)間: 2025-3-28 07:23 作者: SSRIS 時(shí)間: 2025-3-28 14:18
Springer Lab Manualshttp://image.papertrans.cn/a/image/158468.jpg作者: Freeze 時(shí)間: 2025-3-28 16:10 作者: 刺耳的聲音 時(shí)間: 2025-3-28 22:23
s with detailed instructions.Professional antibody engineersInterest in recombinant antibody technologies has rapidly increased because of the wide range of possible applications in therapy and diagnosis, especially in cancer treatment. The possibility of generating human antibodies that are not acc作者: 男生戴手銬 時(shí)間: 2025-3-29 01:41
Einführung in die neue Strafprozessordnungas e. g. in the case of intoxication. In this situation, therapeutic antibodies can be applied to help our own immune system. Further, therapeutic antibodies against self-antigens can be employed to modulate targets not related to an immune challenge, e. g. to interfere with endogenous regulation processes.作者: dissent 時(shí)間: 2025-3-29 07:07
Generation of Antibody Gene Libraries from Seropositive Human Donorsas e. g. in the case of intoxication. In this situation, therapeutic antibodies can be applied to help our own immune system. Further, therapeutic antibodies against self-antigens can be employed to modulate targets not related to an immune challenge, e. g. to interfere with endogenous regulation processes.作者: SOW 時(shí)間: 2025-3-29 08:53
Einführung in die neue Strafprozessordnungcted which combine the two variable regions (Vh and Vl) with a peptide linker to yield an scFv fragment. The genetic information for Vh and Vl is amplified from hybridoma cells using the polymerase chain reaction (PCR) with antibody specific primers.作者: Infant 時(shí)間: 2025-3-29 15:21 作者: 感染 時(shí)間: 2025-3-29 18:03
Einführung in die neue Strafprozessordnung-type pIII proteins. Enrichment of Fab phages with affinity for a specific antigen is then carried out by successive rounds of affinity purification using antigen-coated microtiter wells, immunotubes or plastic beads followed by reinfection of . cells with the eluted bound phages (1-6). An outline of the method is illustrated in Figure 1.作者: 構(gòu)成 時(shí)間: 2025-3-29 21:04
https://doi.org/10.1007/3-211-28109-6en. The numerous reports about the isolation of functional antibody fragments from combinatorial libraries prepared from peripheral blood lymphocytes from immunized or non-immunized human donors exemplify the general applicability of this approach (see Chapter 6, Chapter 7).作者: 過(guò)多 時(shí)間: 2025-3-30 02:56 作者: 環(huán)形 時(shí)間: 2025-3-30 04:53 作者: Flirtatious 時(shí)間: 2025-3-30 10:27 作者: 縮影 時(shí)間: 2025-3-30 16:11
Proximity (ProxiMol) and Step-Back Selections surface antigen alleviating the necessity for labour intensive screening programmes. This selection regime greatly narrows the scope of the selection and increases the probability of selecting antibodies of interest.作者: Lobotomy 時(shí)間: 2025-3-30 16:39
