標題: Titlebook: Animal Coronaviruses; Leyi Wang Book 2016 Springer Science+Business Media New York 2016 Coronaviridae.Reverse genetics.Molecular diagnosti [打印本頁] 作者: 側面上下 時間: 2025-3-21 16:10
書目名稱Animal Coronaviruses影響因子(影響力)
書目名稱Animal Coronaviruses影響因子(影響力)學科排名
書目名稱Animal Coronaviruses網(wǎng)絡公開度
書目名稱Animal Coronaviruses網(wǎng)絡公開度學科排名
書目名稱Animal Coronaviruses被引頻次
書目名稱Animal Coronaviruses被引頻次學科排名
書目名稱Animal Coronaviruses年度引用
書目名稱Animal Coronaviruses年度引用學科排名
書目名稱Animal Coronaviruses讀者反饋
書目名稱Animal Coronaviruses讀者反饋學科排名
作者: JUST 時間: 2025-3-21 21:09
Il ventricolo destro e l’arteria polmonareplete genes. Infectious recombinant IBVs are generated in situ following the transfection of vaccinia virus DNA, containing the modified IBV cDNA, into cells infected with a recombinant fowlpox virus expressing T7 DNA dependant RNA polymerase.作者: obsolete 時間: 2025-3-22 04:00
Ecocardiografia per l‘intensivista spike gene (S2) of TCoV. The assay is highly specific and sensitive and can quantitate between 10. and 10. copies/mL of viral genome. It is useful in monitoring the progression of TCoV-induced atrophic enteritis in the turkey flocks.作者: prostatitis 時間: 2025-3-22 07:01
Reverse Genetics of Avian Coronavirus Infectious Bronchitis Virusplete genes. Infectious recombinant IBVs are generated in situ following the transfection of vaccinia virus DNA, containing the modified IBV cDNA, into cells infected with a recombinant fowlpox virus expressing T7 DNA dependant RNA polymerase.作者: entrance 時間: 2025-3-22 09:31 作者: Canary 時間: 2025-3-22 13:51
1949-2448 ntial for reproducible results.Contains key notes and implem.This detailed volume provides diagnosticians and researchers with practical methodologies and approaches to tackle animal coronaviruses. It explores conventional immunohistochemistry, virus neutralization, enzyme-linked immunosorbent assay作者: Palter 時間: 2025-3-22 17:42
Ecocardiografia per l‘intensivistaogalactopyranoside (IPTG). The expressed N protein inclusion body is extracted and purified by chromatography on nickel-agarose column to near homogeneity. The protein recovery can be 10 mg from 100 ml of bacterial culture. The purified N protein is a superior source of TCoV antigen for antibody-capture ELISA for detection of antibodies to TCoV.作者: 表示向前 時間: 2025-3-22 23:07
Cenni di fisica degli ultrasuoni and efficient protocol for total nucleic acids extraction to be used in conventional RT-PCR assay. This is a technique used routinely in our virology laboratory to detect BCoV from stool and nasopharyngeal samples of cattle.作者: 歌唱隊 時間: 2025-3-23 02:59
Ecocardiografia per l‘intensivistance. It is difficult to diagnose FIP, since the viruses cannot be distinguished using serological or virological methods. Recently, genetic techniques, such as RT-PCR, have been conducted for FIP diagnosis. In this chapter, the reliability of RT-PCR and procedures used to determine FCoV infection as part of antemortem FIP diagnosis is described.作者: 坦白 時間: 2025-3-23 07:17 作者: 教唆 時間: 2025-3-23 10:22
Detection of Bovine Coronavirus by Conventional Reverse Transcription Polymerase Chain Reaction and efficient protocol for total nucleic acids extraction to be used in conventional RT-PCR assay. This is a technique used routinely in our virology laboratory to detect BCoV from stool and nasopharyngeal samples of cattle.作者: 遺產 時間: 2025-3-23 14:04 作者: 吞噬 時間: 2025-3-23 20:32 作者: 偽造 時間: 2025-3-24 00:26
PCR Amplification and Sequencing Analysis of Full-Length Turkey Coronavirus Spike Genetocol on PCR amplification and sequencing analysis of full-length TCoV S gene. Such protocol is useful in molecular epidemiology for establishing an effective strategy to control the transmission of TCoV among turkey flocks.作者: Fillet,Filet 時間: 2025-3-24 05:08
Ecocardiografia per l‘intensivistaethod uses chicken infectious bronchitis coronavirus (IBV) as the coating antigen based on antigenic cross-reactivity between TCoV and IBV. The other method relies on a recombinant TCoV nucleocapsid protein. Both methods are useful for serological diagnosis of TCoV infection in the turkey flocks.作者: 沙文主義 時間: 2025-3-24 08:15
https://doi.org/10.1007/978-88-470-1384-1tocol on PCR amplification and sequencing analysis of full-length TCoV S gene. Such protocol is useful in molecular epidemiology for establishing an effective strategy to control the transmission of TCoV among turkey flocks.作者: Generic-Drug 時間: 2025-3-24 14:43 作者: braggadocio 時間: 2025-3-24 18:16 作者: 破布 時間: 2025-3-24 21:17
Ecocardiografia per l‘intensivistatained for TCoV. In contrast, one PCR product of 356 bp corresponding to a fragment of N gene is obtained for IBV strains and one PCR product of 568 bp corresponding to a fragment of S gene is obtained for BCoV.作者: Pert敏捷 時間: 2025-3-25 01:54
Virus Neutralization Assay for Turkey Coronavirus Infectionum that can inhibit TCoV infection in 50 % of embryonated turkey eggs. Virus neutralization assay for TCoV is useful to monitor the immune status of turkey flocks infected with TCoV for the control of the disease.作者: 滔滔不絕地講 時間: 2025-3-25 06:19 作者: Inkling 時間: 2025-3-25 09:08
A Multiplex Polymerase Chain Reaction for Differential Detection of Turkey Coronavirus from Chicken tained for TCoV. In contrast, one PCR product of 356 bp corresponding to a fragment of N gene is obtained for IBV strains and one PCR product of 568 bp corresponding to a fragment of S gene is obtained for BCoV.作者: 誘惑 時間: 2025-3-25 15:08 作者: 我要威脅 時間: 2025-3-25 19:54
1949-2448 ve, .Animal Coronaviruses. serves as an ideal reference for researchers examining a wide variety of coronavirus species in the .Coronaviridae..978-1-4939-8044-4978-1-4939-3414-0Series ISSN 1949-2448 Series E-ISSN 1949-2456 作者: 愛國者 時間: 2025-3-25 22:58
Immunohistochemical Staining for Detection of Porcine Epidemic Diarrhea Virus in Tissuesas alkaline phosphatase, also gives rise to significant advantages in defining the correlation of viral antigens with histopathologic lesions. PEDV antigens in frozen tissues are visualized as green staining in the cytoplasm of infected cells by fluorescent dyes conjugated with antibodies when activ作者: 吊胃口 時間: 2025-3-26 01:05
An RT-PCR Assay for Detection of Infectious Bronchitis Coronavirus Serotypesostic laboratories. In this chapter, we describe a one-step RT-PCR which can be used for detecting most of IBV serotypes in the IBV-infected allantoic fluid and has been used routinely in our laboratories for detection of IBVs.作者: 全面 時間: 2025-3-26 07:31 作者: disparage 時間: 2025-3-26 09:26 作者: interrogate 時間: 2025-3-26 14:35
Leyi WangIncludes cutting-edge methods and protocols for the study of coronaviruses in domestic animals and poultry.Provides step-by-step detail essential for reproducible results.Contains key notes and implem作者: Cuisine 時間: 2025-3-26 16:47
Springer Protocols Handbookshttp://image.papertrans.cn/a/image/157579.jpg作者: 消息靈通 時間: 2025-3-26 22:39
https://doi.org/10.1007/978-1-4939-3414-0Coronaviridae; Reverse genetics; Molecular diagnostics; Next generation sequencing; Virology; Immunohisto作者: 慢慢流出 時間: 2025-3-27 02:20 作者: EVICT 時間: 2025-3-27 08:03 作者: Perineum 時間: 2025-3-27 11:25 作者: 打擊 時間: 2025-3-27 16:51
Ecocardiografia per l‘intensivistal-time RT-PCRs have been developed for viral identification. In this chapter we describe a one-step real-time RT-PCR that has been routinely used in our laboratory for ECoV detection from fecal and respiratory samples.作者: folliculitis 時間: 2025-3-27 19:04 作者: installment 時間: 2025-3-28 00:45 作者: 來就得意 時間: 2025-3-28 02:07 作者: modest 時間: 2025-3-28 09:20
Cenni di fisica degli ultrasuonine TCoV-specific antibodies are critical to evaluate previous exposure to TCoV in the turkey flocks and differentiate serotypes from different isolates or strains. A specific virus neutralization assay using embryonated turkey eggs and immunofluorescent antibody assay for determining TCoV-specific n作者: Cardioversion 時間: 2025-3-28 12:45 作者: 埋葬 時間: 2025-3-28 15:46 作者: 哥哥噴涌而出 時間: 2025-3-28 19:23
Il ventricolo destro e l’arteria polmonarehe IBV genome is inserted into the vaccinia virus genome under the control of a T7 promoter sequence. Vaccinia virus as a vector for the full-length IBV cDNA has the advantage that modifications can be introduced into the IBV cDNA using homologous recombination, a method frequently used to insert an作者: 表主動 時間: 2025-3-29 01:40
Il ventricolo destro e l’arteria polmonareaordinary large size of the coronaviral genome. The very first reverse genetic system for feline coronaviruses (FCoVs) was established in the early 2000s; the respective approach exclusively enabled the manipulation of the 3′-third of the viral genome. Later on, vaccinia virus- and bacterial artific作者: 俗艷 時間: 2025-3-29 04:42
Ecocardiografia per l‘intensivistal-time RT-PCRs have been developed for viral identification. In this chapter we describe a one-step real-time RT-PCR that has been routinely used in our laboratory for ECoV detection from fecal and respiratory samples.作者: jaunty 時間: 2025-3-29 09:08 作者: nitric-oxide 時間: 2025-3-29 12:44
Cenni di fisica degli ultrasuonid pigs’ feces is critical for controlling the disease outbreaks. The real-time RT-PCR assay described in this chapter can quickly detect the presence of TGEV in fecal samples with high sensitivity and specificity.作者: bonnet 時間: 2025-3-29 17:05
Ecocardiografia per l‘intensivistae poultry production. Since IBV was first described in 1936, many serotypes and variants of IBV have been isolated worldwide. IBV isolates are capable of infecting a large range of epithelial surfaces of the chicken, involving the respiratory, renal, and reproductive systems; however, the clinical s作者: allergen 時間: 2025-3-29 20:37 作者: promote 時間: 2025-3-30 03:11 作者: Trigger-Point 時間: 2025-3-30 04:59
https://doi.org/10.1007/978-88-470-1384-1g and understanding genetic relationships among different TCoV isolates or strains is important for controlling the disease. Using two-step RT-PCR assays that amplify the full length of TCoV spike (S) gene, TCoV isolates can be sequenced, analyzed, and genotyped. Described in this chapter is the pro作者: 保留 時間: 2025-3-30 10:58
Ecocardiografia per l‘intensivista clinical significance; however, a mutation of this avirulent virus (feline enteric coronavirus) to a virulent type (FIP virus) can lead to FIP incidence. It is difficult to diagnose FIP, since the viruses cannot be distinguished using serological or virological methods. Recently, genetic techniques作者: Resistance 時間: 2025-3-30 13:19
Animal Coronaviruses: A Brief Introductionimal species including domestic and wild animals. In this chapter, we provide a brief review on animal CoVs by discussing their receptor, host specificity, reverse genetics, and emerging and re-emerging porcine CoVs.作者: instill 時間: 2025-3-30 17:59 作者: 不舒服 時間: 2025-3-30 21:48
Real-Time Reverse Transcription Polymerase Chain Reaction for Rapid Detection of Transmissible Gastrd pigs’ feces is critical for controlling the disease outbreaks. The real-time RT-PCR assay described in this chapter can quickly detect the presence of TGEV in fecal samples with high sensitivity and specificity.作者: Alveoli 時間: 2025-3-31 04:03
