標(biāo)題: Titlebook: Animal Cell Technology: From Target to Market; Proceedings of the 1 Elisabeth Lindner-Olsson,Nathalie Chatzissavidou,E Conference proceedin [打印本頁] 作者: HEMI 時(shí)間: 2025-3-21 16:48
書目名稱Animal Cell Technology: From Target to Market影響因子(影響力)
書目名稱Animal Cell Technology: From Target to Market影響因子(影響力)學(xué)科排名
書目名稱Animal Cell Technology: From Target to Market網(wǎng)絡(luò)公開度
書目名稱Animal Cell Technology: From Target to Market網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Animal Cell Technology: From Target to Market被引頻次
書目名稱Animal Cell Technology: From Target to Market被引頻次學(xué)科排名
書目名稱Animal Cell Technology: From Target to Market年度引用
書目名稱Animal Cell Technology: From Target to Market年度引用學(xué)科排名
書目名稱Animal Cell Technology: From Target to Market讀者反饋
書目名稱Animal Cell Technology: From Target to Market讀者反饋學(xué)科排名
作者: CBC471 時(shí)間: 2025-3-21 23:17 作者: 圖表證明 時(shí)間: 2025-3-22 01:23
Fermented Milk, Kefram-Kefir Enhances Glucose Uptake into Insulin-Responsive Muscle Cellscorrelated to the excessive production of reactive oxygen species (ROS). Then, we attempted to evaluate whether fermented milk, Kefram-Kefir known as an antioxidant reduces the cellular ROS levels and can stimulate the glucose uptake in L6 skeletal muscle cells. The insulin-responsive differentiated作者: 聾子 時(shí)間: 2025-3-22 07:28
Neurite Outgrowth Promoting Substance from a Marine Alga, ,ctor (NGF) because it can respond to NGF by extending neurites and differentiating into sympathetic neurone-like phenotype (.). A subline of PC 12 cells (PC12D cells) was found to be capable of responding to NGF in a faster rate. They only take 24–48 h to extend neurite after being exposed to NGF (.作者: cortisol 時(shí)間: 2025-3-22 11:27
Neutralization of the Biological Activity of Glycosylated and Non-Glycosylated hGM-CSF by Monoclonalecovery after cancer chemoterapy. Nevertheless, there is increasing evidence of spontaneous human anti-GM-CSF antibodies associated with chronic infections, autoimmune diseases or simply their occurrence in normal sera that could compromise the clinical efficacy of the therapy. The specificity of th作者: 顛簸下上 時(shí)間: 2025-3-22 14:12
High Cell Density Cultivation of Mast Cells in Fluidized-Bed and Fixed-Bed Bioreactorss investigated. Due to the higher mechanical stress in the fluidized-bed and the weak adhesion forces of mast cells, a long-term immobilization in the fluidized-bed was not possible, shown by dielectric spectroscopy. In a fixed-bed bioreactor a continuous fermentation was performed, with a steady-st作者: Spartan 時(shí)間: 2025-3-22 17:05
Over-Expression of Protein Kinase Bα Enhances Recombinant Protein Expression in Transient Systemseasingly acute. Transient expression of recombinant protein using COS-1, CV-1 and 293 cells is widely used to address this need. To improve the robustness of host cells for transient expression, the effect of over-expression of Protein Kinase Bα has been explored. In this report we demonstrate that 作者: Libido 時(shí)間: 2025-3-22 23:43 作者: Lumbar-Spine 時(shí)間: 2025-3-23 01:55
Application of Alphavirus Vectors in Drug Discoveryed in overexpression of topologically different recombinant proteins in a broad range of mammalian host cells. The same replication-deficient alphavirus particles have been subjected to . gene delivery studies. These features have made alphavirus vectors attractive for various approaches in drug dis作者: configuration 時(shí)間: 2025-3-23 07:25
The Use of , Vectors in Combination with a Preadapted Serum Free, Suspension Cell Line Allows for Rafficient expression can be derived from a single copy of an integrated gene site resulting in a higher percentage of cells expressing the marker gene in the selected pool in comparison to standard non-UCOE containing vectors. This, in combination with the utilization of a serum free, suspension adap作者: 華而不實(shí) 時(shí)間: 2025-3-23 10:06
Overexpression of a Transcription Factor to Maximize Glycoprotein Production in an Inducible Expressession system (Ko et al 1989, Gu et al., 1996) in mouse L cells. MMTV-LTR is activated by glucocorticoid hormones through the interaction of the activated hormone-receptor complex with glucocorticoid response element (GRE) (.). When MMTV promoter is stably introduced into host cells, the long termin作者: Instrumental 時(shí)間: 2025-3-23 14:19
Successful Transfection of Partially Purified DNA in the HEK293EBNA Cell Line for Transient Expressiaminants are widely regarded to decrease transfection efficiency or induce cytotoxic effects, (.), (.), (.). Purifying sufficient plasmid DNA is one of the major time consuming and often costly steps prior to performing transfection. This problem is of course increased further when one performs larg作者: 剝削 時(shí)間: 2025-3-23 18:31
Capillary-Aided Cell Cloningnique has been shown to permit the resolution of a mixed cell culture to give monoclonal colonies. The high probability of monoclonality obtained using this technique means that only one round of cloning is required, resulting in time and cost savings in cell line development for therapeutic protein作者: 遺留之物 時(shí)間: 2025-3-23 23:29 作者: mighty 時(shí)間: 2025-3-24 04:43
Eco-Stats: Data Analysis in Ecologycopy..We demonstrate that transient transfection can be done at the 100-liter scale, thus providing a new tool to produce hundreds of milligrams or even gram amounts of recombinant protein. A key advantage of large scale TGE lies in its speed. In the presented case, the entire production process for作者: BANAL 時(shí)間: 2025-3-24 07:47
New Frontiers in Translation Studiesexpression of cDNA libraries and the use of vector for anfisense and ribozyme applications. Preliminary studies in animal models have indicated that alphaviruses are potentially attracdve for gene therapy applications.作者: Indolent 時(shí)間: 2025-3-24 12:42
https://doi.org/10.1007/978-981-15-2260-4rotocol which is generic for the parent cell line. The increased efficiency obtained with the UCOE vector significantly reduces the number of transfectants which need to be screened in order to obtain a high productivity subclone..The transfection of a standard host cell line, which is preadapted to作者: initiate 時(shí)間: 2025-3-24 17:17
https://doi.org/10.1007/978-94-007-0369-8cruitment of the transcriptional factors is reported to be dynamic and not static (.). The bound hormone-receptor complex is disengaged from its binding site through an ATP dependent chromatin remodeling process (.), causing the inactivation of transcription of downstream genes. In order to continuo作者: commune 時(shí)間: 2025-3-24 21:17 作者: Cumulus 時(shí)間: 2025-3-25 00:46 作者: 現(xiàn)實(shí) 時(shí)間: 2025-3-25 04:28 作者: 吼叫 時(shí)間: 2025-3-25 08:49 作者: Ventilator 時(shí)間: 2025-3-25 12:37
Overexpression of a Transcription Factor to Maximize Glycoprotein Production in an Inducible Expresscruitment of the transcriptional factors is reported to be dynamic and not static (.). The bound hormone-receptor complex is disengaged from its binding site through an ATP dependent chromatin remodeling process (.), causing the inactivation of transcription of downstream genes. In order to continuo作者: Tidious 時(shí)間: 2025-3-25 15:55 作者: 座右銘 時(shí)間: 2025-3-25 22:19
Elisabeth Lindner-Olsson,Nathalie Chatzissavidou,E作者: 索賠 時(shí)間: 2025-3-26 00:59 作者: 勾引 時(shí)間: 2025-3-26 05:43
Eco-Socialism For Now and the Futuren augmented the uptake of glucose in L6 skeletal muscle cells both in the presence and absence of insulin stimulation. Estimation of intracellular ROS level by fluorescent dye DCFH-DA revealed that the water-soluble fraction of Kefram-Kefir reduced the intracellular ROS level on both undifferentiated and differentiated L6 cells.作者: 小教堂 時(shí)間: 2025-3-26 11:35
Destabilization of Green Fluorescent Protein by Substitution of Its Amino-Terminal Residues the role of the proteins N-terminal residue, which determines the proteins half-life. The set of destabilizing amino-terminal residues is organized hierarchically (.), with Arg (R) and Phe (F) generating maximum destabilization.作者: 飾帶 時(shí)間: 2025-3-26 12:40
Fermented Milk, Kefram-Kefir Enhances Glucose Uptake into Insulin-Responsive Muscle Cellsn augmented the uptake of glucose in L6 skeletal muscle cells both in the presence and absence of insulin stimulation. Estimation of intracellular ROS level by fluorescent dye DCFH-DA revealed that the water-soluble fraction of Kefram-Kefir reduced the intracellular ROS level on both undifferentiated and differentiated L6 cells.作者: prick-test 時(shí)間: 2025-3-26 20:52
,The Historical Context — Key Trends,ls (PC12D cells) was found to be capable of responding to NGF in a faster rate. They only take 24–48 h to extend neurite after being exposed to NGF (.). Accordingly, PC12D cells are ideal for rapid screening of large quantity of samples for NGF-potentiating substance.作者: 結(jié)果 時(shí)間: 2025-3-26 22:40 作者: Hamper 時(shí)間: 2025-3-27 03:46
Eco-Stats: Data Analysis in Ecologyness of host cells for transient expression, the effect of over-expression of Protein Kinase Bα has been explored. In this report we demonstrate that over-expression of PKBα can improve transient recombinant protein expression 40% to >200% depending on the protein being expressed and the cell line used.作者: 未完成 時(shí)間: 2025-3-27 06:07
Eco-Urbanism and the South East Asian Cityg this technique means that only one round of cloning is required, resulting in time and cost savings in cell line development for therapeutic protein production. This technique has been used to clone hybridoma, CHO and NS0 cell lines.作者: Aerate 時(shí)間: 2025-3-27 10:16
Neurite Outgrowth Promoting Substance from a Marine Alga, ,ls (PC12D cells) was found to be capable of responding to NGF in a faster rate. They only take 24–48 h to extend neurite after being exposed to NGF (.). Accordingly, PC12D cells are ideal for rapid screening of large quantity of samples for NGF-potentiating substance.作者: 濕潤 時(shí)間: 2025-3-27 15:47 作者: 漂白 時(shí)間: 2025-3-27 18:24 作者: 廣口瓶 時(shí)間: 2025-3-27 22:23
Capillary-Aided Cell Cloningg this technique means that only one round of cloning is required, resulting in time and cost savings in cell line development for therapeutic protein production. This technique has been used to clone hybridoma, CHO and NS0 cell lines.作者: 不容置疑 時(shí)間: 2025-3-28 04:25
Anti-Tumor Activity of Noguchi Catalyser 21?, a Mineral Water Containing Natural Leaf Soilhe production of anti-tumor cytokines. RT-PCR analysis revealed that the transcription of IL-12 p40 subunit was significantly enhanced in murine macrophage cell line J774-1, RAW264 and BALB/c mouse-derived peritoneal cavity macrophage. This suggests that enhancement of the tumor immune systems via IL-12 production may be enhanced by Catalyser.作者: STENT 時(shí)間: 2025-3-28 07:26
High Cell Density Cultivation of Mast Cells in Fluidized-Bed and Fixed-Bed Bioreactorsate cell density 38 times higher than in suspension batch cultivation. The immobihzed ceUs were continuously proHferating, analogous to the exponential growth phase in batch fermentation, proven by cell cycle analysis. An interleukin-3 concentration of 2 ng/ml has been found to be sufficient to support optimal cell growth.作者: 濕潤 時(shí)間: 2025-3-28 13:06
Successful Transfection of Partially Purified DNA in the HEK293EBNA Cell Line for Transient Expressie-scale transient transfection for rapid recombinant protein production (.). The transfectability of a DNA extract containing considerable host cell contaminants was assessed, using the calcium phosphate method (.) and HEK293 cells. Impure plasmid DNA proved to be as transfectable as pure preparations in this widely used cell line.作者: accessory 時(shí)間: 2025-3-28 18:02 作者: inscribe 時(shí)間: 2025-3-28 21:07
An Important Equivalence Resultate cell density 38 times higher than in suspension batch cultivation. The immobihzed ceUs were continuously proHferating, analogous to the exponential growth phase in batch fermentation, proven by cell cycle analysis. An interleukin-3 concentration of 2 ng/ml has been found to be sufficient to support optimal cell growth.作者: Panacea 時(shí)間: 2025-3-29 02:58 作者: Transfusion 時(shí)間: 2025-3-29 06:32
Overview: 978-94-010-3897-3978-94-010-0369-8作者: 揉雜 時(shí)間: 2025-3-29 09:55
https://doi.org/10.1007/978-94-010-0369-8Expression; development; drug discovery; gene therapy; metabolism; physiology; protein; regulation作者: 斑駁 時(shí)間: 2025-3-29 12:57 作者: intention 時(shí)間: 2025-3-29 18:58 作者: Anguish 時(shí)間: 2025-3-29 22:02
https://doi.org/10.1007/978-3-319-93680-2 However, this stability limits its apphcation in studies which require rapid reporter turnover for example studies of transient kinetics. Here a shortened half-life would be beneficial in accurately measuring the kinetics of transient mRNA transcription from regulated promoters. In previous work Li作者: 用不完 時(shí)間: 2025-3-30 01:00 作者: emulsify 時(shí)間: 2025-3-30 06:24
Eco-Socialism For Now and the Futurecorrelated to the excessive production of reactive oxygen species (ROS). Then, we attempted to evaluate whether fermented milk, Kefram-Kefir known as an antioxidant reduces the cellular ROS levels and can stimulate the glucose uptake in L6 skeletal muscle cells. The insulin-responsive differentiated作者: 范圍廣 時(shí)間: 2025-3-30 11:57
,The Historical Context — Key Trends,ctor (NGF) because it can respond to NGF by extending neurites and differentiating into sympathetic neurone-like phenotype (.). A subline of PC 12 cells (PC12D cells) was found to be capable of responding to NGF in a faster rate. They only take 24–48 h to extend neurite after being exposed to NGF (.作者: 分解 時(shí)間: 2025-3-30 13:55
Eco-Stats: Data Analysis in Ecologyecovery after cancer chemoterapy. Nevertheless, there is increasing evidence of spontaneous human anti-GM-CSF antibodies associated with chronic infections, autoimmune diseases or simply their occurrence in normal sera that could compromise the clinical efficacy of the therapy. The specificity of th作者: 虛度 時(shí)間: 2025-3-30 20:02 作者: 江湖郎中 時(shí)間: 2025-3-30 22:41 作者: HIKE 時(shí)間: 2025-3-31 03:11 作者: 道學(xué)氣 時(shí)間: 2025-3-31 08:49
New Frontiers in Translation Studiesed in overexpression of topologically different recombinant proteins in a broad range of mammalian host cells. The same replication-deficient alphavirus particles have been subjected to . gene delivery studies. These features have made alphavirus vectors attractive for various approaches in drug dis作者: Preamble 時(shí)間: 2025-3-31 09:51
