作者: 證明無罪 時間: 2025-3-21 22:55
https://doi.org/10.1007/978-3-662-06810-6 density were used to induce aggregate formation. Optical sectioning with confocal microscopy revealed that non-viable cells were predominantly localized in the interior, especially in large sized aggregates. These results indicate that growth kinetics may differ in aggregates of different size. The作者: MURKY 時間: 2025-3-22 02:26
Peter Holleczek,Birgit Vogel-Heusere have established culture conditions for selective formation of floating spheroids on polylysine-coated surfaces. Also, suitable surface characteristics for stable reattachment and for high expression of functions of spheroids re-attached were investigated.作者: 灰姑娘 時間: 2025-3-22 06:53 作者: Adj異類的 時間: 2025-3-22 08:59 作者: 食物 時間: 2025-3-22 13:28 作者: Kidney-Failure 時間: 2025-3-22 18:07 作者: peritonitis 時間: 2025-3-22 21:32 作者: 幾何學家 時間: 2025-3-23 02:26 作者: 萬靈丹 時間: 2025-3-23 08:59
,Ein/Ausgabe für Fortgeschrittene,nhibited by serum proteins such as albumin. Adsorption of serum proteins clearly plays an important role in cell-solid surface phenomena. However, they make difficult our understanding of these problems.作者: Jargon 時間: 2025-3-23 10:55 作者: HEED 時間: 2025-3-23 15:28 作者: 祖?zhèn)髫敭a(chǎn) 時間: 2025-3-23 18:09 作者: Arthropathy 時間: 2025-3-23 23:46
Effect of Protein Adsorption on Cell Attachment on Solid Surfacesnhibited by serum proteins such as albumin. Adsorption of serum proteins clearly plays an important role in cell-solid surface phenomena. However, they make difficult our understanding of these problems.作者: 解凍 時間: 2025-3-24 06:21 作者: 鈍劍 時間: 2025-3-24 07:44
Friedrich Gierlinger,Tobias Lausbergd other properties are distributed unevenly within the aggregates and are affected by the size of the aggregates. Thus, the variation in growth characteristics in aggregates of different size can conceivably lead to oscillations in cell concentration in a perfusion bioreactor. This phenomenon was analyzed using Monte Carlo simulation techniques.作者: Femish 時間: 2025-3-24 14:10 作者: ANTH 時間: 2025-3-24 17:11
al section deals with thebiological properties of proteins produced by engineered animal cells.All those involved in the culture of animal cells will find thisvolume invaluable. .978-94-010-5263-4978-94-011-2844-5作者: formula 時間: 2025-3-24 22:58 作者: ARCH 時間: 2025-3-24 23:19
Conference proceedings 1992ifferent cell culture systems are examined andinvestigations into the factors influencing cell growth andproductivity are presented. A special section deals with thebiological properties of proteins produced by engineered animal cells.All those involved in the culture of animal cells will find thisv作者: judiciousness 時間: 2025-3-25 06:53 作者: Aboveboard 時間: 2025-3-25 07:41 作者: STANT 時間: 2025-3-25 12:26
Jan-Gerrit Jaeger,Dietmar Tutschecies. We demonstrate the stability of fingerprints of cell lines during repeated sub culture, mycoplasma infection (with two species) and high level antibiotic treatment. This data contributes to the validation of DNA fingerprinting in cell technology and promotes its use for routine quality control of cell stocks of diverse species of origin.作者: Dysarthria 時間: 2025-3-25 19:40
,Ein/Ausgabe für Fortgeschrittene,improved their attachment and subsequent growth. Poor attachment of some cell types has been impeding their growth on these microcarriers. An increase in charge density to improve cell attachment may facilitate the cultivation of these cells on macroporous gelatin microcarriers.作者: 食物 時間: 2025-3-25 22:43
https://doi.org/10.1007/978-3-322-84846-8f hepatocyte/spheroid immobilized in the PUF packed-bed were higher than those of usual hepatocyte/monolayer culture in petri dish, and these activities were maintained for 26 days. These results indicated that the PUF/spheroid packed-bed culture system provided a promising prototype of hybrid type artificial liver support system.作者: neutralize 時間: 2025-3-26 02:13
Klaus Hengsbach,Reinhard Langmannhollow fiber bioreactors. Another important design issue is the maintenance of oxygen supply as these reactors are scaled-up for commercial production. Improved design and operating strategies are being developed to both decrease polarization of cell growth and increase hollow fiber bioreactor productivities.作者: synovitis 時間: 2025-3-26 04:34 作者: 同步信息 時間: 2025-3-26 12:16 作者: 拒絕 時間: 2025-3-26 13:37 作者: Projection 時間: 2025-3-26 17:13 作者: 玉米 時間: 2025-3-27 00:27 作者: Noisome 時間: 2025-3-27 04:01
Engineering of Ultrafiltration Hollow Fiber Bioreactors for Mammalian Cell Protein Productionhollow fiber bioreactors. Another important design issue is the maintenance of oxygen supply as these reactors are scaled-up for commercial production. Improved design and operating strategies are being developed to both decrease polarization of cell growth and increase hollow fiber bioreactor productivities.作者: Arb853 時間: 2025-3-27 06:44
Detection of Mammalian Cell Attachment and Lysis on a Metal Surface Using a Piezoelectric Sensorthe sensor. The piezoelectric sensor was successfully applied to detect cell lysis caused by infection of Vesicular Stomatitis Virus. Such a sensor is potentially useful in the detection of cytopathic and stimulatory effects on cell growth.作者: Coronation 時間: 2025-3-27 09:58 作者: Mindfulness 時間: 2025-3-27 15:21 作者: Engulf 時間: 2025-3-27 20:12
Virus Removal and Inactivation in Process Validationric membrane. The necessary condition for employing filters with a polymeric membrane in the purification process is that the filter should be “validatable”. The definition of the validatable membrane is proposed as the membrane which has the reproducibility and the predictability of virus removal. 作者: Adjourn 時間: 2025-3-27 23:09 作者: OTHER 時間: 2025-3-28 05:35
Alteration of Cell Surface Glycoproteins through Differentiation from a Human Histiocytic Lymphoma Cline, U-M was examined. U-M cells were agglutinated by lectins such as concanavalin A (Con A), phytohemogglutinin (PHA-L) and jacalin, while U-937 cells were not, indicating that U-M cells had N-acetylgalactosamine residue at the terminus of complex types of N-linked glycoproteins and O-linked sialo作者: 紅潤 時間: 2025-3-28 07:35
Removability of Virus Particles and Permeability of Protein from Cell Culture Medium Using Cuprammonrmeability of particles and molecules. Gold particles (GP) for model particles and actual viruses were employed. Dead end filtration using BMM was carried out under constant transmembrane pressure (TMP) of 200mmHg. Linear relationships between logarithmic rejection coefficient φ and particle size fo作者: molest 時間: 2025-3-28 13:21 作者: 食品室 時間: 2025-3-28 18:37 作者: 妨礙 時間: 2025-3-28 22:44 作者: Misnomer 時間: 2025-3-28 23:16
Characterization of Cell Growth and Improvement of Attachment Kinetics on Macroporous Microcarriersused to “optically section” microcarriers and visualize the distribution of cells within them. In most microcarriers, cells were found to populate majority of the region close to the surface and some regions in the interior. However, a significant portion of the interior was not occupied. HepG2 cell作者: PHON 時間: 2025-3-29 07:02 作者: Amnesty 時間: 2025-3-29 10:03 作者: 影響帶來 時間: 2025-3-29 12:16
Selective Formation and Immobilization by Re-Attachment of Floating Spheroids of Rat Hepatocytesf the cells have many clinical and industrial applications, including an artificial liver. Adult rat hepatocytes inoculated on positively-charged surfaces were reported to form spheroidal aggregates (spheroids), and they expressed higher hepatic functions in the longer term as compared to monolayer 作者: Innovative 時間: 2025-3-29 17:56
Engineering of Ultrafiltration Hollow Fiber Bioreactors for Mammalian Cell Protein Productiontion for months at high productivities. However, the heterogeneous nature of these bioreactors requires special considerations for proper design and operation. Downstream polarization of both cell growth and high molecular weight proteins occurs as a result of Starling flow in the immobilized cell r作者: Countermand 時間: 2025-3-29 20:14
Production of hEPO by Recombinant C127 Cells using Hollow Fiber Culture Systemls in collagen gel. The EPO productivity in this improved system was six times as high as that without collagen gel. The properties of the extended cells after 5 months culture weren’t different from those of the master bank’s cells. It was shown that this immobilizing technology is suitable for the作者: 過分自信 時間: 2025-3-30 02:45
Oscillatory Behavior in Continuous Aggregate Cultures of Mammalian Cellsf settling is affected by the size of the aggregates. Using confocal microscopy and thin sectioning techniques, we have demonstrated that viability and other properties are distributed unevenly within the aggregates and are affected by the size of the aggregates. Thus, the variation in growth charac作者: 知道 時間: 2025-3-30 04:39 作者: 植物群 時間: 2025-3-30 11:55 作者: 長矛 時間: 2025-3-30 13:47
https://doi.org/10.1007/978-94-011-2844-5bioprocess engineering; cell; cell culture; protein; proteins; system作者: JOT 時間: 2025-3-30 19:48 作者: initiate 時間: 2025-3-30 23:31 作者: Communicate 時間: 2025-3-31 04:37
Production of hEPO by Recombinant C127 Cells using Hollow Fiber Culture Systemls in collagen gel. The EPO productivity in this improved system was six times as high as that without collagen gel. The properties of the extended cells after 5 months culture weren’t different from those of the master bank’s cells. It was shown that this immobilizing technology is suitable for the production of biopharmaceuticals.作者: exceptional 時間: 2025-3-31 05:30
Strategies for Economic GMP Production of Mammalian Cell Expressed Proteinsd posttranslational modification at high fidelity. Unfortunately, mammalian cell culture is more expensive than bacterial or yeast fermentation. It is therefore important to identify cost reduction possibilities in the manufacture of biopharmaceuticals that require production in mammalian host cells.作者: 小口啜飲 時間: 2025-3-31 10:38
Conference proceedings 1992ifferent cell culture systems are examined andinvestigations into the factors influencing cell growth andproductivity are presented. A special section deals with thebiological properties of proteins produced by engineered animal cells.All those involved in the culture of animal cells will find thisvolume invaluable. .作者: 詞匯 時間: 2025-3-31 16:26
Anthropologische Untersuchungen,ubject area. It concludes that there is yet need to develop more refined and explicit conceptual models of a qualitative nature in order that available mathematical techniques can be applied with enhanced benefit for the advancement of the technology.作者: Dislocation 時間: 2025-3-31 19:32 作者: 走路左晃右晃 時間: 2025-4-1 00:29 作者: locus-ceruleus 時間: 2025-4-1 03:06
Jan-Gerrit Jaeger,Dietmar Tutschtogenetics. While fingerprinting does not identify the species of origin it does give specific identification of a cell line. As an alternative, single locus probes can be useful in distinguishing very closely related cell lines. However, they lack the capacity of multilocus DNA fingerprinting where作者: CRAFT 時間: 2025-4-1 06:12 作者: Barrister 時間: 2025-4-1 14:12 作者: –FER 時間: 2025-4-1 14:29
