作者: 印第安人 時間: 2025-3-21 21:25 作者: ECG769 時間: 2025-3-22 01:32 作者: 公司 時間: 2025-3-22 05:21
Intracellular Nucleotide Pools for Optimizing Product-Oriented Transient Transfection of Hek293 Cell transfection efficiency of about 80 % was achieved. The NTP/U ratio has been shown to detect the age of the cell line and to monitor the transfection process. It revealed a high energy level and an exponential growth as best prerequisite for obtaining a high productivity in transiently transfected HEK293 cells in suspension culture.作者: –scent 時間: 2025-3-22 09:21 作者: aggravate 時間: 2025-3-22 12:57
Conference proceedings 2005 that the emerging technologies in the area of genomics, proteomics and other "-omics"-type disciplines will provide key technological assets to increase knowledge and open new horizons in animal cell technology. During the meeting a variety of top-class emerging technologies were presented together作者: 淡紫色花 時間: 2025-3-22 18:54
Conducting a Cardiac Ultrasound Examinationn (GFP), as a marker-protein, fused to the C-terminal portion of ASB. In addition the original ASB signal sequence was included in order to provide correct folding and targeting. The effect of the leader sequence of plasmids and glycosylation was investigated in terms of product location and quality. Transfections were performed in COS cells.作者: 共和國 時間: 2025-3-22 21:26 作者: 使腐爛 時間: 2025-3-23 03:45 作者: 阻擋 時間: 2025-3-23 06:12
Expression Profiling Analysis of Sodium Butyrate-Induced Chinese Hamster Ovary Cells in Defined Medire achieved. However, these agents have the ultimate effect of blocking cell proliferation, and eventually inducing apoptosis in treated cells. We have undertaken an expression profiling approach to identify the genes up and down-regulated in induction conditions.作者: Meager 時間: 2025-3-23 11:38 作者: 深陷 時間: 2025-3-23 15:15 作者: 小卒 時間: 2025-3-23 21:42
Effect of Osmotic Pressure on Gs-Ns0 Expression System and hypo-osmotic cultures. Further differences have been identified in calcium accumulation, metabolism of glucose, glutamate and lactate. Additionally it is shown that there are over 600 genes involved in ion transport, accumulation of osmolytes, cell cycle distribution, proliferation, cytoskeleta作者: 松馳 時間: 2025-3-23 23:29 作者: 逗它小傻瓜 時間: 2025-3-24 04:42 作者: defile 時間: 2025-3-24 06:39 作者: giggle 時間: 2025-3-24 14:29
Analysis of Multiple Apoptosis Parameters Using a Microfluidic Chip-Based Systemndary antibody. Importantly, the small microfluidic chip-based system used here requires only 20,000 cells per sample for the flow cytometric analysis and allows for on-chip optimization of the staining..Results obtained with the microfluidic chips showed good correlation with data obtained using a 作者: Insul島 時間: 2025-3-24 17:08 作者: Rebate 時間: 2025-3-24 23:02 作者: Binge-Drinking 時間: 2025-3-25 00:12
Engineering , (High-Five?) Insect Cells to Express a Cytosolic Pyruvate Carboxylase Enzyme Improves longed time, a major advantage in high cell density cultures especially when combined with stable insect cell lines. These changes in the metabolism were further evaluated in 3L-bioreactor studies, under controlled conditions. The implications of these changes to the operation conditions have been e作者: IST 時間: 2025-3-25 05:53 作者: conformity 時間: 2025-3-25 08:47 作者: 艱苦地移動 時間: 2025-3-25 12:17
The Functional Competence of Animal Cells: Anal Ysis of the Secretory Pathway作者: Mendicant 時間: 2025-3-25 17:06
Correlation of Intracellular Nucleotide Pools to Amino Acid Concentrations in Culture Media by the A作者: Mri485 時間: 2025-3-25 21:45
Antiapoptotic Activity of Synthetic and Nnatural Peptides作者: 新義 時間: 2025-3-26 02:14 作者: PET-scan 時間: 2025-3-26 04:35
Machine Controls and Optimizing the Imagesn of a co-transfected reporter gene. These results demonstrate that epigenetic factors do play a role in stable and transient transgene expression and that molecular approaches can be developed to relieve the negative effects of these factors on recombinant protein expression in mammalian cells.作者: adj憂郁的 時間: 2025-3-26 10:04
Alaa Mabrouk Salem Omar,Partho P. Senguptason with known mouse genes indicates only a moderate degree of homology. The results represent a significant step toward large-scale gene expression profiling for industrial mammalian cell culture processes.作者: Preserve 時間: 2025-3-26 13:55
The Role of Stress in Valvular Heart Diseaseral immune response, multiply presented IgA glycans bind pathogenic bacteria and specific glycoforms of IgG can, when clustered, activate the complement pathway through engaging the mannose binding lectin. The recognition of an unusual cluster of mannose residues on the HIV gp120 envelope glycoprote作者: IRATE 時間: 2025-3-26 20:12 作者: intellect 時間: 2025-3-26 22:06 作者: 偏見 時間: 2025-3-27 01:12
Principles of Hemodynamic Assessmentstudy shows the potential of the proteomics approach in understanding intracellular and physiological changes in cells and seeking a better insight into possible environmental or genetic manipulation approaches for increasing foreign protein production in rCHO cells.作者: Gerontology 時間: 2025-3-27 07:42 作者: 走調 時間: 2025-3-27 13:10 作者: 商議 時間: 2025-3-27 15:51
Hakimeh Sadeghian,Zahra Savand-Roomiongly affected by osmotic conditions. However, because hyper- and hypo-osmotic pressure suppresses cell growth, the enhanced final product concentration of the culture is not observed. Therefore by understanding the basic cellular processes of a GS-NS0 mammalian cell culture system would not only as作者: Radiation 時間: 2025-3-27 20:38
Machine Controls and Optimizing the Imagesre we demonstrate the feasibility of using molecular approaches to overcome the potentially negative effects of these factors. Chinese hamster ovary cells (CHO) were co-transfected with the CELO adenovirus Gam1 gene and either the enhanced green fluorescent protein (EGFP) gene or the IgG heavy and l作者: 盡忠 時間: 2025-3-28 00:33
Alaa Mabrouk Salem Omar,Partho P. Senguptaar basis for these traits at the genomic level is not well understood. A better understanding of key genetic alterations related to these traits will greatly facilitate the development of new cell lines and new processes. To advance this goal, we are constructing a CHO cDNA microarray. A phage libra作者: 帶來的感覺 時間: 2025-3-28 04:51
The Role of Stress in Valvular Heart Disease(CDG). In these diseases, where sugars may be entirely absent or incorrectly processed, many proteins are affected [c1] such that their biological functions are compromised. This disorder gives rise to a wide range of severe clinical conditions, including some that involve the immune system. Most of作者: STYX 時間: 2025-3-28 06:43 作者: 擴大 時間: 2025-3-28 13:25
Clinical Echocardiography Prosthetic Valves A readout system capable of two-color fluorescence detection was used to run disposable glass chips with various analytical functionalities. The chips contain an interconnected network of microchannels that permits either electrophoretic separations of biomolecules or simple flow cytometric analysi作者: 阻擋 時間: 2025-3-28 17:22 作者: incisive 時間: 2025-3-28 21:58 作者: COWER 時間: 2025-3-29 00:36 作者: 兵團 時間: 2025-3-29 07:04 作者: 改良 時間: 2025-3-29 08:22
Cor Pulmonale and Pulmonary Hypertensioning to increase the glycolytic flux into the TCA. This work summarises the effect of yeast pyruvate carboxylase 2 (PYC2) expressed in the cytoplasm of different host systems: BHK-21, CHO-K1, CHO-DG44, HEK293 and BTI-Tn-5B1-4 (High Five?) cells.作者: hemophilia 時間: 2025-3-29 13:22
Three-Dimensional Echocardiographyactate in the medium seems to increase its accumulation by the cells and inhibits cell growth. At the end of culture, after glucose exhaustion from the medium, High-Five? cells are able to use the produced lactate as a carbon source.作者: 整潔漂亮 時間: 2025-3-29 16:14
Inferior and Superior Venae Cavaet quality. In this work we have investigated the metabolism of these cells with respect to the utilization of glucose and glutamine and we have used a metabolic engineering approach to overcome this problem demonstrating the successful culture of these cells at high cell densities. The cells have be作者: Affluence 時間: 2025-3-29 21:35
https://doi.org/10.1007/978-1-84882-027-2The growth of a Sf9 cell line was studied in each of these media, and IPL-41 showed the best results. Nevertheless, this medium contains various carbohydrates as carbon and energy sources, and this fact supposes a complication for the monitoring of the culture. In order to make possible the control 作者: Gentry 時間: 2025-3-30 03:57
Univentricular Heart (Singe Ventricle),idoma cell line (KB 26.5). Our results indicate that the over-expression of cellular . and viral . (from Epstein Barr virus positive cell lines) and . (from Kaposi sarcoma tissues) genes under apoptosis triggering conditions (i. e., glutamine depletion) not only delays cell death process but also al作者: 不可知論 時間: 2025-3-30 06:08
ESACT Proceedingshttp://image.papertrans.cn/a/image/157561.jpg作者: 疏忽 時間: 2025-3-30 09:09
Cor Pulmonale and Pulmonary Hypertensioning to increase the glycolytic flux into the TCA. This work summarises the effect of yeast pyruvate carboxylase 2 (PYC2) expressed in the cytoplasm of different host systems: BHK-21, CHO-K1, CHO-DG44, HEK293 and BTI-Tn-5B1-4 (High Five?) cells.作者: STRIA 時間: 2025-3-30 16:23
Three-Dimensional Echocardiographyactate in the medium seems to increase its accumulation by the cells and inhibits cell growth. At the end of culture, after glucose exhaustion from the medium, High-Five? cells are able to use the produced lactate as a carbon source.作者: 銼屑 時間: 2025-3-30 16:46 作者: 離開 時間: 2025-3-30 23:37
Environmental Effects of Lactate on High-Five? Insect Cell Metabolismactate in the medium seems to increase its accumulation by the cells and inhibits cell growth. At the end of culture, after glucose exhaustion from the medium, High-Five? cells are able to use the produced lactate as a carbon source.作者: 新字 時間: 2025-3-31 01:13 作者: 小卒 時間: 2025-3-31 05:46
Springer Science+Business Media B.V. 2005作者: 倔強不能 時間: 2025-3-31 10:19 作者: MUMP 時間: 2025-3-31 16:25 作者: CAMEO 時間: 2025-3-31 19:22
Genomic Exploration on Chinese Hamster Ovary Cellsar basis for these traits at the genomic level is not well understood. A better understanding of key genetic alterations related to these traits will greatly facilitate the development of new cell lines and new processes. To advance this goal, we are constructing a CHO cDNA microarray. A phage libra作者: ARBOR 時間: 2025-3-31 22:09
Roles for Glycosylation in Receptor-Ligand Interactions in the Immune System(CDG). In these diseases, where sugars may be entirely absent or incorrectly processed, many proteins are affected [c1] such that their biological functions are compromised. This disorder gives rise to a wide range of severe clinical conditions, including some that involve the immune system. Most of作者: 清真寺 時間: 2025-4-1 01:57 作者: BRIDE 時間: 2025-4-1 07:51 作者: 有雜色 時間: 2025-4-1 12:45 作者: HUMP 時間: 2025-4-1 16:41
Proteome Analysis of Recombinant CHO Cells Under Hyperosmotic Stresso-dimensional electrophoresis and mass spectrometry, a proteome profile of rCHO cells comprising 23 identified proteins was established. Based on this proteome profile, we found 3 proteins of which expression levels were significantly changed at 450 mOsm/kg. Compared to the results at 300 mOsm/kg, t作者: finite 時間: 2025-4-1 20:15
Expression Profiling Analysis of Sodium Butyrate-Induced Chinese Hamster Ovary Cells in Defined Medi the desired product to even higher levels. A typical strategy employed by the biotech industry is to express the protein of interest in Chinese Hamster Ovary (CHO) cells stably transfected with the gene. Where even higher levels of expression are desired, inducing agents such as sodium butyrate or
