標(biāo)題: Titlebook: Animal Cell Electroporation and Electrofusion Protocols; Jac A. Nickoloff Book 1995 Springer Science+Business Media New York 1995 [打印本頁] 作者: Nutraceutical 時(shí)間: 2025-3-21 19:15
書目名稱Animal Cell Electroporation and Electrofusion Protocols影響因子(影響力)
書目名稱Animal Cell Electroporation and Electrofusion Protocols影響因子(影響力)學(xué)科排名
書目名稱Animal Cell Electroporation and Electrofusion Protocols網(wǎng)絡(luò)公開度
書目名稱Animal Cell Electroporation and Electrofusion Protocols網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Animal Cell Electroporation and Electrofusion Protocols被引頻次
書目名稱Animal Cell Electroporation and Electrofusion Protocols被引頻次學(xué)科排名
書目名稱Animal Cell Electroporation and Electrofusion Protocols年度引用
書目名稱Animal Cell Electroporation and Electrofusion Protocols年度引用學(xué)科排名
書目名稱Animal Cell Electroporation and Electrofusion Protocols讀者反饋
書目名稱Animal Cell Electroporation and Electrofusion Protocols讀者反饋學(xué)科排名
作者: 拋媚眼 時(shí)間: 2025-3-21 22:46
Instrumentationuration, and wave form. In this chapter, the term electro cell manipulation (ECM) shall describe both techniques. ECM is a quite universal technique that can be applied to eggs, sperm, platelets, mammalian cells, plant protoplasts, plant pollen, liposomes, bacteria, fungi, and yeast—generally to any作者: heterogeneous 時(shí)間: 2025-3-22 03:02
The Introduction of Proteins into Mammalian Cells by Electroporationtroporation, or after transfection of cells with expression vectors containing the gene(s) of interest. Unlike most of these other methods, electroporation is a simple, relatively inexpensive, and efficient method for rapidly introducing DNA, small molecules, and proteins into bacterial or mammalian作者: 混合物 時(shí)間: 2025-3-22 05:08
Electroporation of Antigen-Presenting Cells for T-Cell Recognition and Cytotoxic T-Lymphocyte Priminadicating tumor cells (.–.). CTL normally recognize endogenously processed peptide antigens (Ag) complexed to MHC class I molecules (.,.). Immunization with virus-infected antigen-presenting cells (APC) or Ag-expressing transfectants, and even APC osmotically loaded with antigens can elicit CD8. cyt作者: 整體 時(shí)間: 2025-3-22 10:40 作者: 橢圓 時(shí)間: 2025-3-22 13:32 作者: osculate 時(shí)間: 2025-3-22 21:01
Electrotransformation of Chinese Hamster Ovary Cellsy are easy to culture, they grow rapidly without density dependence, and they do not have stringent culture medium or high serum growth requirements. CHO cell lines have a high plating efficiency, and many lines can grow either as a monolayer or in suspension. These features, and techniques for repl作者: SYN 時(shí)間: 2025-3-22 22:16
Electroporation of Rat Pituitary Cellshis technique has also been used for important functional studies in the different GH cell strains: rat pituitary tumor cells that produce prolactin or growth hormone. In most cases, transient-expression experiments have been used to study cellular processes, such as rat growth hormone gene expressi作者: intuition 時(shí)間: 2025-3-23 02:40
Electroporation of Plasmid DNA into Normal Human Fibroblastsoften been used because of their finite life-span. Instead, immortal human cell lines have usually been used as genetic recipients. These cell lines derived from tumors or by transformation with SV40 virus have accumulated numerous genetic changes (.,.), and may in fact contain changes that will aff作者: 衣服 時(shí)間: 2025-3-23 06:01
Electroporation-Mediated Gene Transfer into Hepatocytese been developed that utilize retroviruses (.), polycations (.), liposomes (.), chromosomes (.), reconstituted viral envelopes (.) and other chemical reagents, such as calcium phosphate (.), DEAE-dextran (.), and lipopolyamines (.). DNA also can be transferred into cells by physical means, such as m作者: Exposure 時(shí)間: 2025-3-23 13:33
Electroporation of Human Lymphoblastoid Cellsoducts on DNA replication (.–.), repair (.–.), recombination (.), or gene expression (.–.) can be performed. Alternatively, vectors that specifically respond to a particular enzymatic function, such as homologous recombination (.,.) or mismatch repair (.), can be introduced to determine whether a ce作者: fulcrum 時(shí)間: 2025-3-23 16:36 作者: Incumbent 時(shí)間: 2025-3-23 18:47
Electroporation of Embryonic Stem Cells for Generating Transgenic Mice and Studying In Vitro Differeot until about 10 years ago did the true potential for the genetic manipulation of cells become a reality, with the ability to alter a mammalian gene precisely by targeted homologous recombination (.–.). This powerful tool was first devised for gene therapy approaches, but was quickly adopted for th作者: 精密 時(shí)間: 2025-3-24 01:53 作者: 上釉彩 時(shí)間: 2025-3-24 05:38
Effect of Cis-Located Human Satellite DNA on Electroporation Efficiency genes containing appropriate expression signals will, in most cases, be expressed both prior to and after integration. However, stable expression of the transfected genes is not always observed (.,.). One reason this is believed to occur is because the site of integration of the foreign DNA is virt作者: 聰明 時(shí)間: 2025-3-24 07:38 作者: 殺菌劑 時(shí)間: 2025-3-24 14:12
Stable Integration of Vectors at High Copy Number for High-Level Expression in Animal Cellsl to the study and the therapeutic utility of a wide variety of proteins. . expression systems are useful for many proteins. However, large complex proteins are not expressed efficiently in bacteria. Also, bacterial expression cannot be used for eukaryotic proteins that require glycosylation for the作者: Lumbar-Spine 時(shí)間: 2025-3-24 16:32 作者: 逗留 時(shí)間: 2025-3-24 19:43
Gordian Schudt,Olga Dolnik,Stephan Beckerully under otherwise mild conditions; and to insert membrane macromolecules into the cell membrane itself. Only recently has the exploration of in vivo electroporation for use with intact tissue begun. Several possible applications have been identified, viz. combined electroporation and anticancer d作者: genesis 時(shí)間: 2025-3-25 02:43 作者: coagulation 時(shí)間: 2025-3-25 07:15 作者: cutlery 時(shí)間: 2025-3-25 10:39
Echinoderms of the Cuban Archipelago,that cells are exquisitely sensitive to the composition of the electroporation medium (.–.) and that an electroporation medium that resembles the composition of the cytoplasm would probably enhance the viability of the cells after electroporation.作者: epidermis 時(shí)間: 2025-3-25 14:21
Electroporation Theoryully under otherwise mild conditions; and to insert membrane macromolecules into the cell membrane itself. Only recently has the exploration of in vivo electroporation for use with intact tissue begun. Several possible applications have been identified, viz. combined electroporation and anticancer d作者: squander 時(shí)間: 2025-3-25 19:44
Electroporation of Adherent Cells , for the Introduction of Nonpermeant Molecules adherent cells, it is imperative to deliver the pulse while the cells are attached to their solid substratum. This has been achieved using a variety of methods (.,.). In this chapter, we describe a technique where cells are grown and electroporated on a glass surface coated with electrically conduc作者: 小說 時(shí)間: 2025-3-25 23:12
Electrotransformation of Chinese Hamster Ovary CellsA damage and repair, recombination, cellular transformation, and protein compartmentalization. Many of these studies have utilized transfection technology to introduce selectable vectors and genes into CHO cell lines.作者: 綁架 時(shí)間: 2025-3-26 02:09
Electrotransfection with “Intracellular” Bufferthat cells are exquisitely sensitive to the composition of the electroporation medium (.–.) and that an electroporation medium that resembles the composition of the cytoplasm would probably enhance the viability of the cells after electroporation.作者: 刺穿 時(shí)間: 2025-3-26 07:09 作者: Spinal-Tap 時(shí)間: 2025-3-26 09:30 作者: 小母馬 時(shí)間: 2025-3-26 13:32
Animal Cell Electroporation and Electrofusion Protocols作者: 平躺 時(shí)間: 2025-3-26 17:14 作者: 殺蟲劑 時(shí)間: 2025-3-26 22:54
Electroporation of Embryonic Stem Cells for Generating Transgenic Mice and Studying In Vitro Differee transfection (.) and genetic disruption of genes in embryonic stem (ES) cell lines to produce transgenic animals (.,.). Currently, the most common cell line used is the mouse ES cell, but ES cells have recently been derived for other species (rabbit, rat, and hamster) (.–.).作者: Conspiracy 時(shí)間: 2025-3-27 01:44
Effect of Cis-Located Human Satellite DNA on Electroporation Efficiencyually random with respect to both the exogenous DNA and the particular human chromosome (.). Therefore, it can be expected that not only the expression, but also the stability and copy number of the transfected gene will depend on the integration sites and chromosomal flanking sequences.作者: Myelin 時(shí)間: 2025-3-27 06:44 作者: 夸張 時(shí)間: 2025-3-27 09:29 作者: 變異 時(shí)間: 2025-3-27 16:24 作者: 魔鬼在游行 時(shí)間: 2025-3-27 20:07
Hans Dieter Klenk,Werner Slenczka cells (.–.). Electroporation has the advantage over other techniques in that many cells can be treated simultaneously and one or more compounds or macromolecules, such as proteins, can be introduced into cells at the same time (..).作者: Accord 時(shí)間: 2025-3-28 01:50 作者: GULF 時(shí)間: 2025-3-28 03:25
P. Flammang,R. Santos,D. Haesaertsually random with respect to both the exogenous DNA and the particular human chromosome (.). Therefore, it can be expected that not only the expression, but also the stability and copy number of the transfected gene will depend on the integration sites and chromosomal flanking sequences.作者: 蒸發(fā) 時(shí)間: 2025-3-28 08:40
P. Flammang,R. Santos,D. Haesaertsir activity. Some established animal cell lines can be used as production hosts since they are easily transfected, can grow to high cell densities, and can be propagated in low-serum or serum-free medium.作者: 嚴(yán)重傷害 時(shí)間: 2025-3-28 11:53 作者: RAG 時(shí)間: 2025-3-28 18:17
1064-3745 broad range of biological studies, from biochemistry and biophysics to molecular biology, cell biology, and whole animal stud- ies. Gene transfer technology in particular will continue to play an essential role in studies aimed at improving our understanding of the relationships between the gene st作者: 芭蕾舞女演員 時(shí)間: 2025-3-28 21:18
Gordian Schudt,Olga Dolnik,Stephan Beckerhat can be applied to eggs, sperm, platelets, mammalian cells, plant protoplasts, plant pollen, liposomes, bacteria, fungi, and yeast—generally to any vesicle surrounded by a membrane. The term “cells” will be used representatively for any of the vesicles to be manipulated unless specific requirements dictate otherwise.作者: cornucopia 時(shí)間: 2025-3-29 01:19
The Biology and Ecology of , Speciesr growth hormone. In most cases, transient-expression experiments have been used to study cellular processes, such as rat growth hormone gene expression (.), prolactin promotor activity (.–.), and the transcription regulatory properties of hormones, such as thyroid hormones and retinoic acid (.–.).作者: 財(cái)政 時(shí)間: 2025-3-29 04:46
https://doi.org/10.1007/978-3-319-18156-1respond to a particular enzymatic function, such as homologous recombination (.,.) or mismatch repair (.), can be introduced to determine whether a cell type of interest is positive or negative for that function.作者: faction 時(shí)間: 2025-3-29 09:23 作者: 晚間 時(shí)間: 2025-3-29 13:42
Electroporation of Rat Pituitary Cellsr growth hormone. In most cases, transient-expression experiments have been used to study cellular processes, such as rat growth hormone gene expression (.), prolactin promotor activity (.–.), and the transcription regulatory properties of hormones, such as thyroid hormones and retinoic acid (.–.).作者: frozen-shoulder 時(shí)間: 2025-3-29 16:47 作者: eulogize 時(shí)間: 2025-3-29 21:55 作者: dilute 時(shí)間: 2025-3-30 03:02
The Naming and Classification of , Species can readily be used for stable transfection experiments. However, before initiating an experiment, the life-span of the recipient cell strains should be characterized, and electroporation should not be performed on cells with insufficient proliferative potential remaining.作者: 我不怕犧牲 時(shí)間: 2025-3-30 07:04
The Biology and Ecology of , Speciesapparatus and are technically difficult, whereas others, such as protoplast fusion, retroviral vectors, and liposome fusion, require time-consuming biochemical manipulations. Electroporation, which circumvents most of these problems, has emerged as an effective tool for the transfection of eukaryotic cells in suspensions (for a review, . ref. .).作者: 黑豹 時(shí)間: 2025-3-30 10:01
P. Flammang,R. Santos,D. Haesaertson of transfection by electroporation with a calorimetric or photometric reporter gene assay meets these requirements, and provides a rapid and sensitive method for the study of genetic regulatory elements in many types of cells.作者: Infinitesimal 時(shí)間: 2025-3-30 13:37 作者: daredevil 時(shí)間: 2025-3-30 19:06
Electroporation of Plasmid DNA into Normal Human Fibroblasts can readily be used for stable transfection experiments. However, before initiating an experiment, the life-span of the recipient cell strains should be characterized, and electroporation should not be performed on cells with insufficient proliferative potential remaining.作者: 誘拐 時(shí)間: 2025-3-30 22:16 作者: Deject 時(shí)間: 2025-3-31 01:50 作者: 壟斷 時(shí)間: 2025-3-31 07:18 作者: Aerate 時(shí)間: 2025-3-31 10:43
https://doi.org/10.1007/978-3-319-18156-1croinjection is the only proven route for gene transfer in livestock, the production of transgenic animals is still problematic and inefficient (.), and importantly, microinjection may impair the development of the embryos (.).作者: 有角 時(shí)間: 2025-3-31 14:53 作者: 是剝皮 時(shí)間: 2025-3-31 19:24
The Use of Electroporated Bovine Spermatozoa to Transfer Foreign DNA into Oocytescroinjection is the only proven route for gene transfer in livestock, the production of transgenic animals is still problematic and inefficient (.), and importantly, microinjection may impair the development of the embryos (.).作者: CURB 時(shí)間: 2025-3-31 22:06
978-1-4899-4061-2Springer Science+Business Media New York 1995
