作者: Frisky 時(shí)間: 2025-3-21 21:27
Adaptation of Mammalian Cells to Growth in Serum-Free Medialtivation in suspension culture, without serum, and at high cell densities. Such phenotypes are useful for the production of recombinant proteins and monoclonal antibodies for therapeutic or diagnostic applications. Emphasis will be placed on Chinese hamster ovary (CHO) cells due to their widespread作者: Palatial 時(shí)間: 2025-3-22 01:10 作者: OGLE 時(shí)間: 2025-3-22 04:56
Optimizing Gene Expression in Mammalian Cellsalian cells are essential to perform such modifications correctly, and efficient methods have been established to transduce or transfect them, based on either viral or synthetic vectors, or combinations of both. Several parameters affect the efficiency of expression of the transgene, such as the nat作者: 身心疲憊 時(shí)間: 2025-3-22 09:02 作者: 嘮叨 時(shí)間: 2025-3-22 16:15
Immortalization Strategies for Mammalian Cellsescence. However, when the control mechanisms which limit life span are modified cells can become immortalized i.e. have an unlimited growth potential (.). One such way of subverting these control mechanisms is to express an immortalizing oncogene in normal cells. These proteins override the normal 作者: alabaster 時(shí)間: 2025-3-22 19:01 作者: 變量 時(shí)間: 2025-3-22 23:36
Cell Bank Preparation and Characterizationerties of a cell line or of a product derived from it, it is, therefore, necessary to cryopreserve and characterize a reference stock of cells. Thereafter, throughout a research, development, or manufacturing program cell cultures are discarded after a defined passage interval and the cryopreserved 作者: Gyrate 時(shí)間: 2025-3-23 01:39 作者: right-atrium 時(shí)間: 2025-3-23 09:17
Cell Counting and Viability Measurementst method is by cell counting at regular intervals. In routine cultures this would be performed once a day, which corresponds to the approximate doubling time of mammalian cells during the exponential growth phase. This would establish an overall growth profile of a culture. More frequent counts woul作者: Blemish 時(shí)間: 2025-3-23 09:53
Monitoring Animal Cell Growth and Productivity by Flow Cytometryanalyzed to provide information on the relationship between them. Fluorescently labeled cells can give quantitative data on specific target molecules and their distribution in the cell population. Moreover, subpopulations of cells with specific attributes can be discriminated and physically sorted f作者: critic 時(shí)間: 2025-3-23 14:14 作者: 殘忍 時(shí)間: 2025-3-23 18:14
Nuclear Magnetic Resonance Methods of Monitoring Cell Metabolismsmall organic molecules. The advent of Fourier transform NMR, coupled with the development of superconducting magnets with higher field strengths, opened the technique to a variety of biological and clinical applications. NMR is now a proven technique for monitoring metabolism in diverse systems—iso作者: 下垂 時(shí)間: 2025-3-24 01:02
Culturing Animal Cells in Fluidized Bed Reactorsestablished technique and has been successfully used from laboratory to production scale in stirred tank reactors. However, as the loading of the microcarriers is raised to increase cell density further, adequate mixing of the culture environment by stirring becomes problematic. To achieve high load作者: Antimicrobial 時(shí)間: 2025-3-24 04:08
GPI-Anchored Fusion Proteinse group of proteins is uniquely anchored to the plasma membrane by the covalent attachment of a complex phospolipid anchor to the carboxyl terminus of the protein. This glycosylphosphatidyl inositol (GPI) anchor consists of a hydrophobic phospholipid, phosphatidylinositol, which attaches the carboxy作者: 四指套 時(shí)間: 2025-3-24 08:59
Cytogenetic Characterization of Recombinant Cellsression vector have been integrated, cellular resistance to methotrexate (MTX), a folate analog, is mediated. MTX can therefore be used to select for subclones that have amplified the transfected plasmid sequences to very high copy numbers.作者: aquatic 時(shí)間: 2025-3-24 11:22
DNA Fingerprinting and Characterization of Animal Cellsact, turned out to be subclones of the HeLa cell line. Thus, it is important that all workers using cell lines be aware of this problem and be able to take steps to avoid the use of bogus cell lines This chapter is intended to give the reader an overview of some of the more popular DNA identificatio作者: Fissure 時(shí)間: 2025-3-24 15:26 作者: Frenetic 時(shí)間: 2025-3-24 21:22 作者: Inscrutable 時(shí)間: 2025-3-24 23:25 作者: Clinch 時(shí)間: 2025-3-25 04:59 作者: conformity 時(shí)間: 2025-3-25 09:02
https://doi.org/10.1057/9781137377388act, turned out to be subclones of the HeLa cell line. Thus, it is important that all workers using cell lines be aware of this problem and be able to take steps to avoid the use of bogus cell lines This chapter is intended to give the reader an overview of some of the more popular DNA identificatio作者: 售穴 時(shí)間: 2025-3-25 13:17
Eastern Practices and Nordic Bodiesrally utilized the .P, .C, .H, and .N nuclei (.,.). The basic principles of NMR spectroscopy, which are beyond the scope of this chapter, have been described extensively elsewhere (for example, ...). This chapter will focus on various NMR-based approaches for studying metabolism in cultured mammalia作者: delusion 時(shí)間: 2025-3-25 18:11 作者: 外面 時(shí)間: 2025-3-25 20:01 作者: expansive 時(shí)間: 2025-3-26 01:22 作者: 注意 時(shí)間: 2025-3-26 05:11
Book 19991st editiondvances that have occurred in both the science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in genetically manipulatinganimal cells, optimizing their growth in defined media (particularly at large-scale), avoiding contamination, and in t作者: 詞根詞綴法 時(shí)間: 2025-3-26 10:14
Book 19991st editionalization and cell fusion; and techniques for the study of cell growth, viability, metabolism, and productivity. Animal Cell Biotechnology constitutes a comprehensive manual of state-of-the-art techniques for setting up a cell culture laboratory, maintaining cell lines, and optimizing critical parameters for cell culture. .作者: breadth 時(shí)間: 2025-3-26 16:00
Industrialisation in Eastern Nigeria negative regulators of cell division. Upon being bound by SV40 large T antigen, p53 and Rb are inactivated and the cells have an unlimited life span. There are a number of good reviews on immortalization (.–.) that deal in greater detail with biology involved in the process which is beyond the scope of this review.作者: arthrodesis 時(shí)間: 2025-3-26 19:40 作者: 冒號(hào) 時(shí)間: 2025-3-26 21:06
Immortalization Strategies for Mammalian Cells negative regulators of cell division. Upon being bound by SV40 large T antigen, p53 and Rb are inactivated and the cells have an unlimited life span. There are a number of good reviews on immortalization (.–.) that deal in greater detail with biology involved in the process which is beyond the scope of this review.作者: Conscientious 時(shí)間: 2025-3-27 01:24
Cell Bank Preparation and Characterizationl bank system. This consists of a master cell bank (MCB) of 50–400 ampoules and manufacturer’s working cell banks (WCB) each derived in an identical manner from one ampoule of the MCB (.). In this chapter the methods of preparation, storage, and characterization of such cell banks are described.作者: epidermis 時(shí)間: 2025-3-27 06:04 作者: 蓋他為秘密 時(shí)間: 2025-3-27 09:54
Eastern Practices and Nordic Bodiesare a number of common features, such as cell shrinkage, externalization of phosphatidylserine, nuclear condensation, nuclear fragmentation, and degradation of the DNA into nucleosomal fragments (.–.).作者: ANT 時(shí)間: 2025-3-27 16:18
1940-6061 he major advances that have occurred in both the science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in genetically manipulatinganimal cells, optimizing their growth in defined media (particularly at large-scale), avoiding contamination作者: ablate 時(shí)間: 2025-3-27 18:48 作者: 有特色 時(shí)間: 2025-3-27 23:53 作者: Dictation 時(shí)間: 2025-3-28 05:42 作者: AVOW 時(shí)間: 2025-3-28 07:52 作者: BLAZE 時(shí)間: 2025-3-28 14:25
Eastern Practices and Nordic Bodiesocarriers is raised to increase cell density further, adequate mixing of the culture environment by stirring becomes problematic. To achieve high loading of microcarriers, alternative means of obtaining mass transfer of oxygen, nutrients, and so forth are required.作者: 侵略 時(shí)間: 2025-3-28 18:40
Adaptation of Mammalian Cells to Growth in Serum-Free Mediamonoclonal antibodies for therapeutic or diagnostic applications. Emphasis will be placed on Chinese hamster ovary (CHO) cells due to their widespread use throughout the biotechnology industry. However, the general principles of cell adaptation can be applied to other animal cells, including baby hamster kidney (BHK) and hybridoma cells.作者: 偶像 時(shí)間: 2025-3-28 20:45 作者: 邊緣 時(shí)間: 2025-3-29 00:12 作者: emission 時(shí)間: 2025-3-29 05:32
Cell Counting and Viability Measurementsng time of mammalian cells during the exponential growth phase. This would establish an overall growth profile of a culture. More frequent counts would be required to follow more subtle changes that may, for example, be associated with the cell growth cycle.作者: cylinder 時(shí)間: 2025-3-29 09:47
Culturing Animal Cells in Fluidized Bed Reactorsocarriers is raised to increase cell density further, adequate mixing of the culture environment by stirring becomes problematic. To achieve high loading of microcarriers, alternative means of obtaining mass transfer of oxygen, nutrients, and so forth are required.作者: epinephrine 時(shí)間: 2025-3-29 13:27
https://doi.org/10.1007/978-1-349-00666-3arameters can be optimized to increase gene expression. An approach based on transfection of mammalian cells with DNA consisting of multiple copies of the gene of interest is described in this chapter.作者: Cognizance 時(shí)間: 2025-3-29 17:34 作者: 共同時(shí)代 時(shí)間: 2025-3-29 21:52 作者: intellect 時(shí)間: 2025-3-30 01:23 作者: 商店街 時(shí)間: 2025-3-30 05:53 作者: NOVA 時(shí)間: 2025-3-30 12:05 作者: deface 時(shí)間: 2025-3-30 13:20 作者: allergen 時(shí)間: 2025-3-30 17:26 作者: Indent 時(shí)間: 2025-3-30 20:46 作者: Adherent 時(shí)間: 2025-3-31 01:08 作者: crease 時(shí)間: 2025-3-31 07:17
