作者: OTTER 時(shí)間: 2025-3-21 22:24 作者: 無彈性 時(shí)間: 2025-3-22 03:28
Ralf P?rtnerIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: 天空 時(shí)間: 2025-3-22 08:09 作者: 小步舞 時(shí)間: 2025-3-22 10:12 作者: irreparable 時(shí)間: 2025-3-22 15:02 作者: 保守黨 時(shí)間: 2025-3-22 18:12 作者: CANDY 時(shí)間: 2025-3-22 21:32 作者: PATRI 時(shí)間: 2025-3-23 02:26
https://doi.org/10.1007/978-1-349-22299-5edge of cell requirements increased steadily. In the beginning, animal-sourced components were the key to growth. Nowadays state-of-the-art media do not contain any animal or naturally sourced components. The compositions are based on scientific awareness of the needs of the cells. The result is hig作者: originality 時(shí)間: 2025-3-23 06:29
https://doi.org/10.1007/978-1-349-22299-5epletion of important medium components and results in improved culture longevity and high cell and product yields. To take maximum advantage of these effects, it is important to optimize the fed-batch process for each application. In this chapter, a simple strategy for fed-batch optimization is des作者: extract 時(shí)間: 2025-3-23 12:33
https://doi.org/10.1007/978-1-349-22299-5 supplements are crucial to protein productivity, medium optimization can be labor intensive and time-consuming. In this chapter, we describe some basic concepts in medium development and introduce a rational and rapid workflow to screen and optimize media and feeds. The major goal of medium screeni作者: Fluctuate 時(shí)間: 2025-3-23 17:48 作者: acclimate 時(shí)間: 2025-3-23 19:40 作者: CRUMB 時(shí)間: 2025-3-24 02:15
https://doi.org/10.1007/978-3-319-77069-7ility in a bioprocess include microscopic counting, electronic particle counting, image analysis, in situ biomass monitoring, and dieletrophoretic cytometry. These methods work most simply when a fixed volume sample can be taken from a suspension culture. Manual microscopic counting is laborious but作者: RUPT 時(shí)間: 2025-3-24 04:53 作者: BAIL 時(shí)間: 2025-3-24 06:56 作者: DEBT 時(shí)間: 2025-3-24 11:02 作者: 轉(zhuǎn)向 時(shí)間: 2025-3-24 16:09
Eastern Europe in the Postwar Worldton fingerprint of each molecule. Here we describe a protocol for exometabolome analysis of mammalian cells using this technique, including sample preparation, spectra acquisition, and integration. The potential of this technique is exemplified by application to cultures of a Chinese hamster ovary (作者: transplantation 時(shí)間: 2025-3-24 22:20
Animal Cell Biotechnology978-1-62703-733-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 吹牛大王 時(shí)間: 2025-3-24 23:28 作者: 偽造 時(shí)間: 2025-3-25 04:59 作者: 苦澀 時(shí)間: 2025-3-25 07:31
Kevin McDermott,Vítězslav Sommerand fed-batch systems. A simplified network of mammalian cell metabolism is used to illustrate the flux estimation procedure, and the steps leading up the consistency index determination are presented. If gross measurement errors are detected, a technique for determining the source of gross measurem作者: Asperity 時(shí)間: 2025-3-25 14:38
Book 2014Latest editiontivation techniques, and Part V describes special applications, including vaccine production, baculovirus protein expression, chromatographic techniques for downstream as well as membrane techniques for virus separation. Written in the successful .Methods in Molecular Biology. series format, chapter作者: Flat-Feet 時(shí)間: 2025-3-25 18:57 作者: insular 時(shí)間: 2025-3-25 23:25 作者: 集中營 時(shí)間: 2025-3-26 01:36 作者: Commemorate 時(shí)間: 2025-3-26 04:28 作者: 卡死偷電 時(shí)間: 2025-3-26 10:45 作者: Indict 時(shí)間: 2025-3-26 16:28 作者: 一個(gè)姐姐 時(shí)間: 2025-3-26 20:45 作者: deceive 時(shí)間: 2025-3-27 00:48 作者: cushion 時(shí)間: 2025-3-27 03:04 作者: 兩棲動(dòng)物 時(shí)間: 2025-3-27 09:12 作者: Arb853 時(shí)間: 2025-3-27 09:44 作者: 支柱 時(shí)間: 2025-3-27 17:39
Screening and Optimization of Chemically Defined Media and Feeds with Integrated and Statistical App supplements are crucial to protein productivity, medium optimization can be labor intensive and time-consuming. In this chapter, we describe some basic concepts in medium development and introduce a rational and rapid workflow to screen and optimize media and feeds. The major goal of medium screeni作者: 小歌劇 時(shí)間: 2025-3-27 20:08
Evaluation of Solid and Porous Microcarriers for Cell Growth and Production of Recombinant Proteinsferation, and therefore improving both cell and product yields obtained in these cultures. The establishment of a successful microcarrier culture depends on many factors, such as the type of microcarrier, the cells, and the culture conditions. In this chapter, the basic steps required for the evalua作者: Nebulizer 時(shí)間: 2025-3-27 23:31
Microbioreactors and Scale-Down Models: Growth of CHO Cells Using the Pall Micro24 MicroReactor Systlopment. Many such devices have been reported in the literature, but only a small number are available commercially. Microbioreactors range in complexity from simple plate-based systems to complex automated parallel bioreactors designed to enable the meaningful scale-down of conventional bioprocesse作者: 沙發(fā) 時(shí)間: 2025-3-28 03:56
Monitoring Cell Growth, Viability, and Apoptosisility in a bioprocess include microscopic counting, electronic particle counting, image analysis, in situ biomass monitoring, and dieletrophoretic cytometry. These methods work most simply when a fixed volume sample can be taken from a suspension culture. Manual microscopic counting is laborious but作者: Distribution 時(shí)間: 2025-3-28 06:59 作者: 怪物 時(shí)間: 2025-3-28 13:43
Quenching Methods for the Analysis of Intracellular Metabolites adherent cells is achieved by application of hot air after removal of the supernatant by suction. For suspension cultures, the addition of excess ice-cold saline results in a rapid inactivation of metabolism and significant dilution of extracellular metabolites. Medium carryover is prevented by rin作者: Notify 時(shí)間: 2025-3-28 17:15
NMR Methods for Metabolomics of Mammalian Cell Culture Bioreactorsroscopy has played an important role, largely because it requires minimal sample preparation, does not require chromatographic separation, and is quantitative. The concentrations of large numbers of small molecules in the extracellular media or within the cells themselves can be measured directly on作者: 藝術(shù) 時(shí)間: 2025-3-28 20:57 作者: ENDOW 時(shí)間: 2025-3-29 01:18 作者: 祖?zhèn)?nbsp; 時(shí)間: 2025-3-29 06:17
https://doi.org/10.1007/978-3-319-77069-7nds on many factors, such as the type of microcarrier, the cells, and the culture conditions. In this chapter, the basic steps required for the evaluation and optimization of a microcarrier culture for the purpose of production of recombinant proteins are described, for both solid and porous microcarriers.作者: Vulnerable 時(shí)間: 2025-3-29 09:15
Eastern Europe in the Postwar Worldparation, spectra acquisition, and integration. The potential of this technique is exemplified by application to cultures of a Chinese hamster ovary (CHO) cell line. The average error associated to this method is under 3% and the limit of quantification for all metabolites analyzed is below 180 μM.作者: innate 時(shí)間: 2025-3-29 12:10
Evaluation of Solid and Porous Microcarriers for Cell Growth and Production of Recombinant Proteinsnds on many factors, such as the type of microcarrier, the cells, and the culture conditions. In this chapter, the basic steps required for the evaluation and optimization of a microcarrier culture for the purpose of production of recombinant proteins are described, for both solid and porous microcarriers.作者: LAY 時(shí)間: 2025-3-29 17:53
1H-NMR Protocol for Exometabolome Analysis of Cultured Mammalian Cellsparation, spectra acquisition, and integration. The potential of this technique is exemplified by application to cultures of a Chinese hamster ovary (CHO) cell line. The average error associated to this method is under 3% and the limit of quantification for all metabolites analyzed is below 180 μM.作者: Motilin 時(shí)間: 2025-3-29 23:25 作者: VAN 時(shí)間: 2025-3-30 02:22
https://doi.org/10.1007/978-1-349-22299-5cribed, consisting of the development of a feed medium based on spent media analysis and the establishment of a feeding strategy that consists of adding variable volumes of feed media at specific intervals, after off-line measurement of the concentration of a reference nutrient.作者: patriarch 時(shí)間: 2025-3-30 06:38 作者: Cholecystokinin 時(shí)間: 2025-3-30 11:59
High-Throughput Synchronization of Mammalian Cell Cultures by Spiral Microfluidicsze fractionation in a spiral microfluidic channel. Protocols for the synchronization of primary cells such as mesenchymal stem cells, and immortal cell lines such as Chinese hamster ovarian cells (CHO-CD36) and HeLa cells are provided as examples.作者: 跑過 時(shí)間: 2025-3-30 13:13
Feed Optimization in Fed-Batch Culturecribed, consisting of the development of a feed medium based on spent media analysis and the establishment of a feeding strategy that consists of adding variable volumes of feed media at specific intervals, after off-line measurement of the concentration of a reference nutrient.作者: POINT 時(shí)間: 2025-3-30 18:19 作者: 猛然一拉 時(shí)間: 2025-3-31 00:01 作者: antenna 時(shí)間: 2025-3-31 04:46
https://doi.org/10.1007/978-1-349-27069-9ction. Cell immobilization on microcarriers is a scalable option to circumvent cell concentration by centrifugation and subsequent dilution or perfusion. Furthermore microcarrier-based cultivation offers a simple solution for medium exchange which allows to maintain cultures during a production period of several weeks.作者: 敲竹杠 時(shí)間: 2025-3-31 05:58 作者: 滔滔不絕的人 時(shí)間: 2025-3-31 10:43
https://doi.org/10.1007/978-1-349-22299-5ribed. The composition is based on public available formulations and information based on the work of many scientists printed in numerous papers and manuscripts. The method shall help beginners to design their own medium, although some knowledge of biochemistry and animal cells is still required.作者: 持續(xù) 時(shí)間: 2025-3-31 16:02
The Impact of the Prague Spring on the USSR,ng and control of temperature, pH, and dissolved oxygen. Inoculation, sampling, and feed additions are carried out manually in a Biological Safety Cabinet. In this chapter we describe the use of the Micro24 system to carry out screening or process development experiments with CHO cells.
