作者: 包庇 時(shí)間: 2025-3-21 20:52 作者: 發(fā)牢騷 時(shí)間: 2025-3-22 03:26
https://doi.org/10.1007/978-3-642-91345-7ools to assist in the assessment of contamination in ancient DNA data sets. These tools perform standard tests of authenticity of ancient DNA data including detecting the presence of postmortem damage signatures in sequence alignments and quantifying the amount of present-day human contamination.作者: Phenothiazines 時(shí)間: 2025-3-22 05:26
Beth Shapiro,Axel Barlow,André E. R.‘SoaresIncludes cutting-edge, fully updated techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts作者: AWRY 時(shí)間: 2025-3-22 12:33 作者: Demonstrate 時(shí)間: 2025-3-22 16:05
https://doi.org/10.1007/978-94-017-6236-6DNA extracted from keratinous and chitinous materials can be a useful source of genetic information. To effectively liberate the DNA from these materials, buffers containing relatively high levels of DTT, proteinase K, and detergent are recommended, followed by purification using either silica column or organic methods.作者: formula 時(shí)間: 2025-3-22 20:08 作者: calumniate 時(shí)間: 2025-3-22 21:57 作者: 原告 時(shí)間: 2025-3-23 01:36 作者: Melanoma 時(shí)間: 2025-3-23 08:48 作者: Hyaluronic-Acid 時(shí)間: 2025-3-23 09:54 作者: 不持續(xù)就爆 時(shí)間: 2025-3-23 16:55 作者: 地牢 時(shí)間: 2025-3-23 19:53
Role of Drebrin in Synaptic Plasticityof ancient human remains are often complicated by contamination with?present-day human DNA, which can be introduced during excavation and subsequent handling of the specimens. In most cases, the relative abundance of contaminant DNA is much greater than that of the target organism. Here we present t作者: 發(fā)源 時(shí)間: 2025-3-24 01:23
Role of Drebrin in Synaptic Plasticity between samples and substrates but usually constitutes only a small fraction of the total DNA, while the remainder comprises contamination deriving from bacteria, fungi, and other microorganisms and in some cases also modern human DNA. Recently, several protocols have been developed to improve acce作者: arbiter 時(shí)間: 2025-3-24 02:49
https://doi.org/10.1007/978-4-431-56550-5ard tissue matrix is degraded using an EDTA/Proteinase K lysis buffer, and the DNA is purified using spin columns with silica membranes. This method efficiently recovers molecules as short as 35 base-pairs long.作者: 平項(xiàng)山 時(shí)間: 2025-3-24 10:35 作者: 情節(jié)劇 時(shí)間: 2025-3-24 10:57
https://doi.org/10.1007/978-94-015-2902-0etics over Quaternary timescales using ancient DNA (aDNA)-based analyses. A variety of plant tissues, preserved primarily by desiccation and anaerobic waterlogging, have proven to be viable sources of aDNA. Plant tissues are anatomically and chemically diverse and therefore require optimized DNA ext作者: JECT 時(shí)間: 2025-3-24 16:01 作者: Soliloquy 時(shí)間: 2025-3-24 22:20 作者: 制定法律 時(shí)間: 2025-3-25 01:26
https://doi.org/10.1007/978-94-015-2808-5 but such methods are still lacking for other sequencing platforms. Here, we provide a protocol for building genomic libraries from degraded DNA (archival or ancient sample material) for sequencing on the Ion Torrent? high-throughput sequencing platforms. In addition to a reduction in time and cost 作者: Chauvinistic 時(shí)間: 2025-3-25 06:21 作者: prosthesis 時(shí)間: 2025-3-25 10:48 作者: 揮舞 時(shí)間: 2025-3-25 14:18
https://doi.org/10.1007/978-3-658-38167-7taminants, such as DNA from living humans. Additionally, some ancient DNA research projects aim to target specific genomic regions, such as mitochondrial genomes or variable single nucleotide polymorphisms (SNPs). To overcome the challenge of targeting specific fragments of DNA from within a complex作者: 領(lǐng)先 時(shí)間: 2025-3-25 17:00
Anwendungen bei Vielteilchensystemen,-extracted “contaminant” DNA from other organisms. One approach to mitigate this challenge is to perform hybridization-based capture of target genomic regions using DNA or RNA baits. Such baits are designed to have high sequence similarity to the target genomic regions and can reduce the off-target 作者: 新陳代謝 時(shí)間: 2025-3-25 21:56 作者: microscopic 時(shí)間: 2025-3-26 03:38
Vektorkugelfunktionen und Ihre Anwendungen,rred to as environmental DNA (eDNA) or sedimentary ancient DNA (sedaDNA), can be recovered from both present-day and ancient soils, fecal samples, bodies of water and lake cores, and even air. While eDNA is a potentially useful record of past and present biodiversity, several challenges complicate d作者: 地名詞典 時(shí)間: 2025-3-26 05:21 作者: 廣大 時(shí)間: 2025-3-26 12:18
https://doi.org/10.1007/978-3-642-91345-7ocess and analyze the sheer amounts of data generated by current aDNA studies and in biomedical research in general. This chapter is intended as a practical guide to the assembly of ancient mitochondrial genomes, directly from genomic DNA-derived next-generation sequencing (NGS) data, specifically i作者: Debark 時(shí)間: 2025-3-26 13:25 作者: 磨坊 時(shí)間: 2025-3-26 19:37
Hiroyuki Yamazaki,Tomoaki Shirao extraction protocols may need to be optimized for the recovery of DNA from sediments, which may contain inhibitors. Here we describe procedures for subsampling both nonfrozen and frozen sediment cores, and we describe an efficient method for ancient DNA extraction from such samples.作者: atopic-rhinitis 時(shí)間: 2025-3-27 00:38
https://doi.org/10.1007/978-94-017-6236-6 are not suitable for aDNA or other types of damaged DNA. Here, we outline a protocol for HTS library preparation that is optimized for ancient DNA. We report the library conversion rate for a range of input template and adapter concentrations. Our results show that the protocol performs at a high efficiency.作者: Soliloquy 時(shí)間: 2025-3-27 05:11 作者: 破布 時(shí)間: 2025-3-27 08:07
Sampling and Extraction of Ancient DNA from Sediments, extraction protocols may need to be optimized for the recovery of DNA from sediments, which may contain inhibitors. Here we describe procedures for subsampling both nonfrozen and frozen sediment cores, and we describe an efficient method for ancient DNA extraction from such samples.作者: licence 時(shí)間: 2025-3-27 13:05 作者: thwart 時(shí)間: 2025-3-27 16:34 作者: 植物茂盛 時(shí)間: 2025-3-27 21:41 作者: hematuria 時(shí)間: 2025-3-28 01:49 作者: 溺愛(ài) 時(shí)間: 2025-3-28 03:33 作者: 種植,培養(yǎng) 時(shí)間: 2025-3-28 09:58
Hiroyuki Yamazaki,Tomoaki Shirao working with ancient samples, including providing guidelines for setting up a new ancient DNA laboratory. We also discuss steps that can be taken at the sample collection and preparation stage to minimize the potential for contamination of ancient DNA experiments with exogenous sources of DNA.作者: 女歌星 時(shí)間: 2025-3-28 11:54
Role of Drebrin in Synaptic Plasticityeach) pretreatment to destroy contaminant DNA that may be bound or otherwise attached to the surface of bone/tooth powder. The second, less destructive pretreatment uses a phosphate buffer to release surface-bound DNA.作者: reception 時(shí)間: 2025-3-28 16:50 作者: alliance 時(shí)間: 2025-3-28 19:58 作者: placebo-effect 時(shí)間: 2025-3-29 02:45
Anwendungen bei Vielteilchensystemen,edium-sized target loci, such as mitochondrial genomes, from ancient DNA extracts. The protocol that we present builds on previously published work by introducing improvements that improve recovery of short DNA fragments while minimizing the cost and duration of the experiment.作者: Occlusion 時(shí)間: 2025-3-29 05:09
Umkopplung: 6,- und 9,-Symbole,of data from large sample sets of poorly preserved specimens, such as fossil remains, forensic samples, and museum specimens. The approach is cost-effective, rapid, and applicable to forensics, population genetics, and phylogenetic research questions.作者: formula 時(shí)間: 2025-3-29 10:12
https://doi.org/10.1007/978-3-642-91345-7reconstruct the mitochondrial genome of a woolly mammoth deposited ~45,000?years ago. We introduce key software tools and outline general strategies for mitogenome assembly, including the critical quality assessment of assembly results without a reference genome.作者: 壓碎 時(shí)間: 2025-3-29 12:17 作者: 原諒 時(shí)間: 2025-3-29 15:44 作者: Connotation 時(shí)間: 2025-3-29 23:07
Pretreatment: Improving Endogenous Ancient DNA Yields Using a Simple Enzymatic Predigestion Step,or multiple washes with weak sodium phosphate or sodium hypochlorite (bleach) solutions, as described in Chapter .. Here, we present an alternative, less aggressive pretreatment protocol that uses a brief predigestion step in an EDTA-based lysis buffer to increase the endogenous fraction prior to extraction.作者: 有毒 時(shí)間: 2025-3-30 01:28 作者: 癡呆 時(shí)間: 2025-3-30 04:37
Application of Solid-State Capture for the Retrieval of Small-to-Medium Sized Target Loci from Anciedium-sized target loci, such as mitochondrial genomes, from ancient DNA extracts. The protocol that we present builds on previously published work by introducing improvements that improve recovery of short DNA fragments while minimizing the cost and duration of the experiment.作者: inconceivable 時(shí)間: 2025-3-30 08:56 作者: enumaerate 時(shí)間: 2025-3-30 12:36 作者: Abduct 時(shí)間: 2025-3-30 18:14
Book 2019Latest editionancient samples for DNA extraction, the DNA extraction itself, and transforming extracted ancient DNA molecules for sequencing library preparation. The volume also examines the analysis of high-throughput sequencing data recovered from ancient specimens, which, because of the degraded nature of anci作者: MUTED 時(shí)間: 2025-3-30 20:57
Extraction of Ancient DNA from Plant Remains,raction approaches. Here, we describe a plant DNA isolation protocol that performs well in most contexts. We include recommendations for optimization to retain the very short DNA fragments that are expected to be preserved in degraded tissues.作者: 流浪 時(shí)間: 2025-3-31 04:50
Sequencing Library Preparation from Degraded Samples for Non-illumina Sequencing Platforms,in comparison to commercial kits, this protocol removes purification steps prior to library amplification, an important consideration for work involving historical samples. Libraries prepared using this method are appropriate for either shotgun sequencing or enrichment-based downstream approaches.作者: Fluctuate 時(shí)間: 2025-3-31 05:31 作者: 犬儒主義者 時(shí)間: 2025-3-31 12:37
Book 2019Latest edition cutting-edge, .Ancient DNA: Methods and Protocols, Second Edition. aims to serve both experts and beginners by presenting protocols in a manner that makes them easily accessible for everyday use in the lab..作者: 本土 時(shí)間: 2025-3-31 17:04
https://doi.org/10.1007/978-94-017-7137-5tion of repetitive regions in the DNA library; and (3) performing in vitro transcription of the repetitive region-depleted DNA library to generate biotinylated RNA baits. Where applicable, we include notes and recommendations based on our own experiences.作者: 溝通 時(shí)間: 2025-3-31 19:04
Vektorkugelfunktionen und Ihre Anwendungen,s (e.g., plants, mammals), using the polymerase chain reaction (PCR) with established generic “barcode” primers. We also provide a catalog of animal and plant barcode primers that, because they target relatively short fragments of DNA, are potentially suitable for use with degraded DNA.作者: 踉蹌 時(shí)間: 2025-3-31 22:04
1064-3745 lls.?.Authoritative and cutting-edge, .Ancient DNA: Methods and Protocols, Second Edition. aims to serve both experts and beginners by presenting protocols in a manner that makes them easily accessible for everyday use in the lab..978-1-4939-9176-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Glossy 時(shí)間: 2025-4-1 02:24
Generating RNA Baits for Capture-Based Enrichment,tion of repetitive regions in the DNA library; and (3) performing in vitro transcription of the repetitive region-depleted DNA library to generate biotinylated RNA baits. Where applicable, we include notes and recommendations based on our own experiences.作者: AMBI 時(shí)間: 2025-4-1 06:17 作者: 沉默 時(shí)間: 2025-4-1 11:27
