標(biāo)題: Titlebook: Amyloid Proteins; Einar M. Sigurdsson Book 20051st edition Humana Press 2005 ELISA.Termination.Vivo.biology.electron microscopy.molecular [打印本頁(yè)] 作者: Hypothesis 時(shí)間: 2025-3-21 17:32
書目名稱Amyloid Proteins影響因子(影響力)
書目名稱Amyloid Proteins影響因子(影響力)學(xué)科排名
書目名稱Amyloid Proteins網(wǎng)絡(luò)公開度
書目名稱Amyloid Proteins網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Amyloid Proteins被引頻次
書目名稱Amyloid Proteins被引頻次學(xué)科排名
書目名稱Amyloid Proteins年度引用
書目名稱Amyloid Proteins年度引用學(xué)科排名
書目名稱Amyloid Proteins讀者反饋
書目名稱Amyloid Proteins讀者反饋學(xué)科排名
作者: 惰性氣體 時(shí)間: 2025-3-21 22:32
,Wie man über ein Problem nachdenken soll,tly produces covalent bonds among unmodified polypeptide chains through . generation of peptide free radicals. PICUP provides a snapshot of the native oligomerization state of proteins and can be used for assembly state analysis of a wide variety of peptides and proteins.作者: placebo 時(shí)間: 2025-3-22 00:26
Wie unterscheiden sich Bildungskonzepte? to PHFs. These include: a purification protocol for recombinantly expressed tau; a general method for the polyanion induced polymerization of tau to PHFs; the quantitation of PHFs by a fluorescence-based assay; the imaging and verification of PHFs by negative stain transmission electron microscopy.作者: deadlock 時(shí)間: 2025-3-22 06:23 作者: 噴出 時(shí)間: 2025-3-22 12:11
https://doi.org/10.1007/3-540-44903-5 easy source of robust cells, and secondly, using brain tissue that provides the proper cerebral VSMCs, but is more problematic to work with. Finally the maintenance, preservation, and characterization of the isolated VSMCs are described.作者: pantomime 時(shí)間: 2025-3-22 15:53 作者: chemical-peel 時(shí)間: 2025-3-22 19:53 作者: Cryptic 時(shí)間: 2025-3-22 23:00 作者: 動(dòng)作謎 時(shí)間: 2025-3-23 01:39 作者: 樹木心 時(shí)間: 2025-3-23 06:17 作者: Preserve 時(shí)間: 2025-3-23 12:35
,Der Künstler – eine prek?re Profession,detailed protocol for the generation of prion-infected cells. We also give step-by-step procedures to test the biochemical properties (mainly protease resistance and insolubility) of abnormal PrP molecules, which detection represents a biochemical marker of prion propagation.作者: FLINT 時(shí)間: 2025-3-23 14:44
1064-3745 n the etiology, pathogenesis, diagnosis, and therapy of amyloid diseases. The contributors provide methods for the preparation of amyloid and its precursors (oligomers and protofibrils), in vitro assays and analytical techniques for their study, and cell culture models and assays for the production 作者: 弄臟 時(shí)間: 2025-3-23 20:16 作者: generic 時(shí)間: 2025-3-23 22:59
Wie unterscheiden sich Wahrheitsbegriffe?ion is one method that has been successfully used to examine the structure of these insoluble fibers. The procedure involves the formation of suitable, ordered amyloid fibrils and characterization (by electron microscopy), partial alignment of fibers, X-ray data collection, data analysis, and finally, model building.作者: 脫落 時(shí)間: 2025-3-24 03:18 作者: ANNUL 時(shí)間: 2025-3-24 07:32
In Vitro Preparation of Prefibrillar Intermediates of Amyloid-β and α Synucleinmyloid fibril formation in the pathogenesis of Alzheimer‘s and Parkinson‘s diseases. In this chapter we discuss several methods, developed by different laboratories, that enable the preparation and stabilization of amyloid-β and α-synuclein protofibrillar species of defined morphologies for biochemical, biophysical and toxicity studies.作者: 輕彈 時(shí)間: 2025-3-24 14:21
X-Ray Diffraction Studies of Amyloid Structureion is one method that has been successfully used to examine the structure of these insoluble fibers. The procedure involves the formation of suitable, ordered amyloid fibrils and characterization (by electron microscopy), partial alignment of fibers, X-ray data collection, data analysis, and finally, model building.作者: intolerance 時(shí)間: 2025-3-24 16:57 作者: expansive 時(shí)間: 2025-3-24 22:48 作者: Visual-Field 時(shí)間: 2025-3-25 02:55 作者: 慢慢啃 時(shí)間: 2025-3-25 06:46
https://doi.org/10.1007/978-3-476-99613-8y the finding that denaturation of the wild-type and variant proteins facilitates a similar folding of both molecules, diminishing their differences in structure and biophysical properties. Following native purification conditions, variant cystatin C has a distinct structure compared to the wild-type protein.作者: CHIDE 時(shí)間: 2025-3-25 08:18
Cyclic Amplification of Protein Misfolding and Aggregationhrough the food chain. In this chapter, we describe the principles behind the PMCA technology, its application, and methodology to detect minute quantities of misfolded prion protein and its potential to be used for amplification of misfolding of other proteins implicated in diverse diseases.作者: 有權(quán)威 時(shí)間: 2025-3-25 13:19
Purification of Human Wild-Type or Variant Cystatin C From Conditioned Media of Transfected Cellsy the finding that denaturation of the wild-type and variant proteins facilitates a similar folding of both molecules, diminishing their differences in structure and biophysical properties. Following native purification conditions, variant cystatin C has a distinct structure compared to the wild-type protein.作者: Pert敏捷 時(shí)間: 2025-3-25 16:35
Preparation of Aggregate-Free,Low Molecular Weight Amyloidβ for Assembly and Toxicity Assays avoid these problems, several methods have been devised that provide reliable means of preparing amyloid-forming proteins for experimental use.Here,we discuss methods that have been used successfully to prepare one such protein,the amyloid #x03B2; protein (Aβ,involved in Alzheimer‘s disease.作者: 切掉 時(shí)間: 2025-3-25 23:43 作者: 白楊魚 時(shí)間: 2025-3-26 00:59
https://doi.org/10.1385/1592598749ELISA; Termination; Vivo; biology; electron microscopy; molecular biology; proteins; reproducible technique作者: Ordeal 時(shí)間: 2025-3-26 06:46
Amyloid Proteins978-1-59259-874-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 我就不公正 時(shí)間: 2025-3-26 09:31 作者: 健壯 時(shí)間: 2025-3-26 15:52 作者: Expurgate 時(shí)間: 2025-3-26 20:21
https://doi.org/10.1007/978-3-658-08630-5tionship among the different intermediates and their relationship to the structure of the amyloid fibrils is critical for understanding the roles of amyloid fibril formation in the pathogenesis of Alzheimer‘s and Parkinson‘s diseases. In this chapter we discuss several methods, developed by differen作者: elastic 時(shí)間: 2025-3-27 00:44
Wie unterscheiden sich Bildungskonzepte?-tau is found in Alzheimer‘s disease brain as insoluble fibers, the so-called “paired helical filaments” (PHFs). Investigating the fundamentals of tau polymerization is indispensable for identifying inhibitory conditions or compounds preventing PHF formation, which may slow down or even stop the deg作者: 案發(fā)地點(diǎn) 時(shí)間: 2025-3-27 02:56
Wie unterscheiden sich Bildungskonzepte? We have recently described a novel technology to amplify cyclically the misfolding and aggregation process in vitro. This procedure, named protein misfolding cyclic amplification (PMCA), conceptually analogous to DNA amplification by PCR, has tremendous implications for research and diagnosis. The 作者: installment 時(shí)間: 2025-3-27 06:01
Wie unterscheiden sich Wahrheitsbegriffe?eposited. Conventional methods of X-ray crystallography and NMR cannot be used, since the fibers are insoluble and heterogeneous. X-ray fiber diffraction is one method that has been successfully used to examine the structure of these insoluble fibers. The procedure involves the formation of suitable作者: 模仿 時(shí)間: 2025-3-27 11:04 作者: filial 時(shí)間: 2025-3-27 13:54 作者: heartburn 時(shí)間: 2025-3-27 18:11 作者: 整潔漂亮 時(shí)間: 2025-3-27 22:25 作者: Dungeon 時(shí)間: 2025-3-28 02:06
Die Kunst des klugen Umgangs mit Konfliktenghts into the prerequisites and kinetics of the polymerization was obtained by the in vitro analysis of this process. In the past, fluorescent dyes were used to stain amyloidogenic material in histology and later on similar dyes were used in in vitro studies as well. To circumvent the flaws of extra作者: 說明 時(shí)間: 2025-3-28 06:38
https://doi.org/10.1007/978-3-642-41779-5s typically used for the purpose of measuring the mass of unknowns. However, the judicious selection of an internal standard permits the quantitative determination of protein concentration. For amyloid formation, this is particularly useful in circumstances where either the protein under study is in作者: 衰老 時(shí)間: 2025-3-28 13:39
https://doi.org/10.1007/3-540-44903-5lated, especially prevalent in Alz-heimer‘ disease (AD), and the main feature of certain hereditary disorders (i.e., HCHWA-I). The vascular smooth muscle cells (VSMCs) appear to play a vital role in the development of CAA, which makes them well-suited as an experimental model to study the disease an作者: 肉體 時(shí)間: 2025-3-28 16:04
https://doi.org/10.1007/978-3-476-99613-8vestigation of cellular mechanisms involved in cerebral amyloid angiopathy (CAA). Difficulties in isolation and propagation of murine cerebrovascular cells and insufficient yields for molecular and cell culture studies have deterred investigators from using mice as a source for cerebrovascular cells作者: SAGE 時(shí)間: 2025-3-28 19:36
https://doi.org/10.1007/978-3-476-99613-8nhibitor cystatin C is the major constituent of the amyloid deposited in the cerebral vasculature of patients with the Icelandic form of hereditary cerebral hemorrhage with amyloidosis (HCHWA-I) (.,.). In order to study the nature of the biophysical changes owing to the Leu68Gln substitution in cyst作者: 評(píng)論性 時(shí)間: 2025-3-29 00:57
,Der Künstler – eine prek?re Profession,ts responsible of transmissible spongiform encephalopathies. As a matter of fact, prion-infected cell lines are very valuable to investigate the cell biology of both the normal and the pathological isoform of the prion protein or to develop and screen new therapeutics. In this chapter, we present a 作者: 起草 時(shí)間: 2025-3-29 04:08
Einar M. SigurdssonIncludes supplementary material: 作者: defendant 時(shí)間: 2025-3-29 08:42
Methods in Molecular Biologyhttp://image.papertrans.cn/a/image/154779.jpg作者: visceral-fat 時(shí)間: 2025-3-29 13:30
Preparation of Aggregate-Free,Low Molecular Weight Amyloidβ for Assembly and Toxicity Assayse used widely to study the structure, assembly,and physiologic effects of both oligomeric and fibrillar forms of these proteins. In many cases,conflicting results arise in these studies,in part owing to difficulties in reproducibly preparing amyloidogenic proteins in a well-defined assembly state.To作者: diathermy 時(shí)間: 2025-3-29 18:11
Determination of Peptide Oligomerization State Using Rapid Photochemical Crosslinkingnding the mechanisms of Aβ assembly could prove useful in the identification of therapeutic targets. Owing to the metastable nature of Aβ oligomers, it is difficult to obtain interpretable data through application of classical methods, such as electrophoresis, chromotography, fluorescence, and light作者: 微枝末節(jié) 時(shí)間: 2025-3-29 22:05
In Vitro Preparation of Prefibrillar Intermediates of Amyloid-β and α Synucleintionship among the different intermediates and their relationship to the structure of the amyloid fibrils is critical for understanding the roles of amyloid fibril formation in the pathogenesis of Alzheimer‘s and Parkinson‘s diseases. In this chapter we discuss several methods, developed by differen作者: 入會(huì) 時(shí)間: 2025-3-30 00:55
Purification of Recombinant Tau Protein and Preparation of Alzheimer-Paired Helical Filaments In Vit-tau is found in Alzheimer‘s disease brain as insoluble fibers, the so-called “paired helical filaments” (PHFs). Investigating the fundamentals of tau polymerization is indispensable for identifying inhibitory conditions or compounds preventing PHF formation, which may slow down or even stop the deg作者: 使迷惑 時(shí)間: 2025-3-30 06:20 作者: organic-matrix 時(shí)間: 2025-3-30 09:13 作者: LURE 時(shí)間: 2025-3-30 15:17
Molecular Electron Microscopy Approaches to Elucidating the Mechanisms of Protein Fibrillogenesisses, including Alzheimer‘s disease, Parkinson‘s disease, and prion diseases. In this chapter, we discuss the application of various EM techniques to monitor and characterize quaternary structural changes during amyloid fibril formation in vitro and the potential of extending some of these techniques作者: canonical 時(shí)間: 2025-3-30 18:45
Time -Lapse Atomic Force Microscopy in the Characterization of Amyloid-Like Fibril Assembly and Oligloid-like fibrils and oligomers can be imaged in buffer solutions, allowing the samples to retain physiological-like properties while temporal changes in structure are monitored, e.g., the elongation of fibrils or the growth of single oligomers. These qualities distinguish AFM from conventional imag作者: miscreant 時(shí)間: 2025-3-30 22:31
Fourier Transform Infrared and Circular Dichroism Spectroscopies for Amyloid Studiesf cross ?-sheet structure. Both far-UV circular dichroism and Fourier transform infrared spectroscopy (FTIR) spectroscopies are the most commonly used techniques for determining the secondary structure of proteins and peptides that either have not been or cannot be studied by nuclear magnetic resona作者: 亂砍 時(shí)間: 2025-3-31 02:07
Quasielastic Light Scattering for Protein Assembly Studiess chapter, we discuss the principles and practice of QLS with respect to protein assembly reactions. Particles undergoing Brownian motion produce fluctuations in scattered light intensity. We describe how the temporal correlation function of these fluctuations can be measured and how this correlatio作者: Initiative 時(shí)間: 2025-3-31 08:34
Intrinsic Fluorescent Detection of Tau Conformation and Aggregationghts into the prerequisites and kinetics of the polymerization was obtained by the in vitro analysis of this process. In the past, fluorescent dyes were used to stain amyloidogenic material in histology and later on similar dyes were used in in vitro studies as well. To circumvent the flaws of extra作者: 吞下 時(shí)間: 2025-3-31 11:25 作者: faction 時(shí)間: 2025-3-31 16:39 作者: 標(biāo)準(zhǔn) 時(shí)間: 2025-3-31 21:21
Murine Cerebrovascular Cells as a Cell Culture Model for Cerebral Amyloid Angiopathyvestigation of cellular mechanisms involved in cerebral amyloid angiopathy (CAA). Difficulties in isolation and propagation of murine cerebrovascular cells and insufficient yields for molecular and cell culture studies have deterred investigators from using mice as a source for cerebrovascular cells作者: quiet-sleep 時(shí)間: 2025-3-31 23:28 作者: 誹謗 時(shí)間: 2025-4-1 04:16 作者: AER 時(shí)間: 2025-4-1 07:36
1064-3745 at, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.978-1-61737-502-6978-1-59259-874-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: MEEK 時(shí)間: 2025-4-1 11:38
https://doi.org/10.1007/978-3-658-24696-9of this chapter provides a methodological introduction to AFM, whilst the second details the application of this technique to the investigation of amyloidogenic proteins, specifically amylin and amyloid- ?(A?) peptides
