標題: Titlebook: Amyloid Proteins; Methods and Protocol Einar M. Sigurdsson,Miguel Calero,María Gasset Book 2018Latest edition Springer Science+Business Med [打印本頁] 作者: Cyclone 時間: 2025-3-21 16:54
書目名稱Amyloid Proteins影響因子(影響力)
書目名稱Amyloid Proteins影響因子(影響力)學科排名
書目名稱Amyloid Proteins網(wǎng)絡公開度
書目名稱Amyloid Proteins網(wǎng)絡公開度學科排名
書目名稱Amyloid Proteins被引頻次
書目名稱Amyloid Proteins被引頻次學科排名
書目名稱Amyloid Proteins年度引用
書目名稱Amyloid Proteins年度引用學科排名
書目名稱Amyloid Proteins讀者反饋
書目名稱Amyloid Proteins讀者反饋學科排名
作者: Myelin 時間: 2025-3-21 22:58
1064-3745 oteins: Methods and Protocols, Third Edition. is a valuable resource for both students and scientists who are new to the field, as well as experienced researchers who would like to progress their research with the latest available techniques..978-1-4939-9293-5978-1-4939-7816-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 口訣法 時間: 2025-3-22 02:51
Preparation of Pure Populations of Amyloid β-Protein Oligomers of Defined Sizearbon bonds among the monomers comprising each oligomer, thus stabilizing the oligomers. We then isolated pure populations of oligomers by fractionating the oligomers by size using SDS-PAGE and then extracting each population from the stained gel bands. Although this procedure worked well with the s作者: 人充滿活力 時間: 2025-3-22 05:09 作者: 搬運工 時間: 2025-3-22 09:54
Purification and Characterization of Low-n Tau Oligomersc force microscopy, and others. Functional characterization includes the assessment of synapses and toxicity assays which show that oligomers can damage synapses locally but show little toxicity to neurons globally.作者: 夜晚 時間: 2025-3-22 14:13 作者: Urea508 時間: 2025-3-22 20:05 作者: CHOKE 時間: 2025-3-22 22:37
,Kierkegaard: “Das Musikalisch-Erotische”,ng Western blot and a targeted proteomic analysis which combines immunoprecipitation with MALDI-ToF mass spectrometry. This approach revealed the presence of numerous C- and N-terminal truncated Aβ species in addition to Aβ1-40/42. Notably, the more soluble C-terminal cleaved fragments constitute a 作者: 思想流動 時間: 2025-3-23 03:47
https://doi.org/10.1007/978-3-658-40058-3c force microscopy, and others. Functional characterization includes the assessment of synapses and toxicity assays which show that oligomers can damage synapses locally but show little toxicity to neurons globally.作者: legislate 時間: 2025-3-23 08:33 作者: ambivalence 時間: 2025-3-23 12:41
Generation and Characterization of Stable α-Synuclein Oligomersmethod to generate kinetically stable α-synuclein oligomers by the addition of reactive aldehydes, 4-hydroxy-2-nonenal, and 4-oxo-2-nonenal. We also describe biochemical and immunological techniques to characterize the generated oligomers.作者: 難解 時間: 2025-3-23 17:43
Preparation and Characterization of Tau Oligomer Strainse have developed and optimized techniques to isolate and study oligomeric tau strains both in vitro and ex vivo. Moreover, we have modified protocols that demonstrate the seeding properties of oligomeric tau strains that are capable of propagating in vivo. These methods and protocols are explained in this chapter.作者: Protein 時間: 2025-3-23 19:05
Das Wissenschaftsfach der Motologie,iderable effort has been devoted to understanding the oligomerization process and to determining structure-activity relationships among the many types of oligomers that have been described. We discuss here a method for producing pure populations of amyloid β-protein (Aβ) of specific sizes using the 作者: 搜集 時間: 2025-3-23 22:54
Johann Valentin Andreae: , (1619), disease (AD). To obtain structural information on this type of oligomers, we were inspired by membrane protein approaches used to stabilize, characterize, and analyze the function of such proteins. Using these approaches, we developed conditions under which Aβ42, the Aβ variant most strongly linked作者: FOLLY 時間: 2025-3-24 04:13
,Kierkegaard: “Das Musikalisch-Erotische”,ease (AD). Besides classic full-length peptides, biochemical analyses of brain deposits have revealed high degree of Aβ heterogeneity likely resulting from the action of multiple proteolytic enzymes. This chapter describes a sequential extraction protocol allowing the differential fractionation of s作者: Certainty 時間: 2025-3-24 06:56
https://doi.org/10.1007/978-3-476-04169-2spreading of neurodegenerative diseases, although the nature of the most toxic forms and the mechanisms by which they trigger neurodegeneration remain largely unknown. This is at least in part due to the inherent challenges involved in the preparation of stable and structurally homogeneous samples o作者: 步兵 時間: 2025-3-24 13:17 作者: 肉身 時間: 2025-3-24 17:58 作者: 不愿 時間: 2025-3-24 21:27 作者: 喃喃訴苦 時間: 2025-3-25 01:08 作者: attenuate 時間: 2025-3-25 03:37 作者: CAB 時間: 2025-3-25 10:32
Neue Regeln für einen neuen Kriegd fibril formation process are important for mechanistic studies. We here report protocols for production, purification, and fibrillation of three different proteins commonly found in cerebral amyloid; Aβ and Tau found in Alzheimer’s disease, Chronic traumatic brain injury, Corticobasal degeneration作者: peak-flow 時間: 2025-3-25 14:09
Neue Regeln für einen neuen Kriegnsiderable structural and functional diversity by virtue of their cation-binding properties, and occur as either Ca.-bound or Ca.-free states with distinct aggregation propensities. That is the case among β-parvalbumins and S100 proteins, which under certain conditions undergo Ca.-dependent self-ass作者: 存心 時間: 2025-3-25 17:19
Neue Regeln für einen neuen Kriegn of functional materials. Several different molecular processes acting in concert are responsible for the formation of amyloid fibrils from monomeric protein in solution. Here, we describe a method to determine which microscopic?processes drive the overall formation of fibrils by using chemical kin作者: 藐視 時間: 2025-3-25 20:05 作者: seruting 時間: 2025-3-26 01:08
,Fast-sinusf?rmig schwingende Oszillatoren, principles of fibrillation, and because intermediate species have been suggested as the pathogenic agents in several amyloid diseases. Structural investigations are however greatly complicated by the dynamic changes?between structural states of very different sizes and life-times. Small angle X-ray作者: 打包 時間: 2025-3-26 05:39
,Fast-sinusf?rmig schwingende Oszillatoren,lation of the prion protein and amyloid precursor protein, phosphorylation of tau and α-synuclein. Posttranslational modifications alter physical properties of proteins including their net and surface charges, affecting their processing, life-time and propensity to acquire misfolded, disease-associa作者: minimal 時間: 2025-3-26 11:27 作者: Corroborate 時間: 2025-3-26 13:27 作者: Picks-Disease 時間: 2025-3-26 18:11 作者: Paradox 時間: 2025-3-27 01:01 作者: 一大群 時間: 2025-3-27 04:28 作者: Peculate 時間: 2025-3-27 08:22
978-1-4939-9293-5Springer Science+Business Media, LLC, part of Springer Nature 2018作者: 出來 時間: 2025-3-27 09:28 作者: 發(fā)怨言 時間: 2025-3-27 15:46 作者: 并入 時間: 2025-3-27 20:39 作者: anaphylaxis 時間: 2025-3-28 01:16
1064-3745 ation advice from the expertsThis third edition volume expands on the previous editions with many new chapters that cover the latest techniques and topics that were not addressed in the previous volumes. The chapters in this book are divided into three parts: Part One covers in vitro assays that foc作者: 不可侵犯 時間: 2025-3-28 04:06
https://doi.org/10.1007/978-3-531-94172-1rkinson’s disease, including how to assess the starting material, useful experimental/instrumental conditions, as well as how to set up cross-seeding and co-aggregation experiments. The high variability of data reported for in vitro α-synuclein amyloid formation may in part be explained by experimental differences.作者: 痛苦一生 時間: 2025-3-28 08:55 作者: 下邊深陷 時間: 2025-3-28 11:35
Neue Regeln für einen neuen Kriegetics in combination with systematic experimental datasets?analysed in a global manner. We outline general concepts for obtaining suitable kinetic data and detail the key stages of data analysis, from quality control to the verification of a specific mechanism of aggregation.作者: legislate 時間: 2025-3-28 14:48
https://doi.org/10.1007/978-3-658-40058-3hese arrays use libraries containing partly overlapping peptides derived from the sequence of Gad m 1, the major allergen from Atlantic cod, which forms amyloids under gastrointestinal relevant conditions.作者: Acquired 時間: 2025-3-28 20:02 作者: Inculcate 時間: 2025-3-29 00:01 作者: GRIEF 時間: 2025-3-29 05:00 作者: OVERT 時間: 2025-3-29 07:55
Amplification and Detection of Minuscule Amounts of Misfolded Prion Protein by Using the Real-Time Qnation between different PrP subtypes or strains, understanding the mechanism of protein misfolding and pre-screening of anti-prion drugs. The technique can be further developed to be used to study characteristics of misfolded proteins in other “prion like” diseases, such as tauopathies, synucleinopathies, or amyloidopathies.作者: 預感 時間: 2025-3-29 14:40
In Vitro Analysis of α-Synuclein Amyloid Formation and Cross-Reactivityrkinson’s disease, including how to assess the starting material, useful experimental/instrumental conditions, as well as how to set up cross-seeding and co-aggregation experiments. The high variability of data reported for in vitro α-synuclein amyloid formation may in part be explained by experimental differences.作者: 道學氣 時間: 2025-3-29 16:05
Purification and Fibrillation of Recombinant Human Amyloid-β, Prion Protein, and Tau Under Native Co, and Progressive Supranuclear Palsy and human prion protein found in Creutzfeldt-Jakob’s disease. The three protocols have in common that the protein is in a pH-neutral phosphate saline buffer during fibrillation to mimic their endogenous near physiological environment.作者: ascend 時間: 2025-3-29 20:49 作者: 帳單 時間: 2025-3-30 01:56 作者: 無聊的人 時間: 2025-3-30 06:23
Book 2018Latest editionin the previous volumes. The chapters in this book are divided into three parts: Part One covers in vitro assays that focus on a variety? of amyloids and how to study these peptides and proteins. Part Two describes cell culture models and assays, and Part Three explores methods on how to extract amy作者: 種族被根除 時間: 2025-3-30 09:30
Johann Valentin Andreae: , (1619),cteristics of a β-barrel arrangement. We named this oligomer β-barrel Pore-Forming Aβ42 Oligomer (βPFO.). Here, we describe detailed protocols for its preparation and characterization. We expect βPFO. to be useful in establishing the involvement of membrane-associated Aβ oligomers in AD.作者: Obvious 時間: 2025-3-30 16:10
https://doi.org/10.1007/978-3-476-04169-2d assemblies, namely a kinetically trapped oligomeric species and a propagating-competent fibrillar polymorph. The degree of heterogeneity in the samples has been defined and carefully minimized, thus allowing for meaningful structure-toxicity relationships in different α-synuclein amyloid assemblies to be established.作者: 改良 時間: 2025-3-30 18:30 作者: Gorilla 時間: 2025-3-31 00:43
Neue Regeln für einen neuen Kriegtein and in fish Gad m 1 allergenic protein, which are implicated in human disease processes. Here, we describe detailed methods to generate and monitor the formation of amyloidogenic assemblies and aggregates of these two EF-hand proteins in vitro.作者: 故意釣到白楊 時間: 2025-3-31 03:52 作者: LUMEN 時間: 2025-3-31 08:33 作者: 手銬 時間: 2025-3-31 10:05
Preparation of α-Synuclein Amyloid Assemblies for Toxicity Experimentsd assemblies, namely a kinetically trapped oligomeric species and a propagating-competent fibrillar polymorph. The degree of heterogeneity in the samples has been defined and carefully minimized, thus allowing for meaningful structure-toxicity relationships in different α-synuclein amyloid assemblies to be established.作者: 思想靈活 時間: 2025-3-31 16:57 作者: 果仁 時間: 2025-3-31 21:13
Preparation of Amyloidogenic Aggregates from EF-Hand β-Parvalbumin and S100 Proteinstein and in fish Gad m 1 allergenic protein, which are implicated in human disease processes. Here, we describe detailed methods to generate and monitor the formation of amyloidogenic assemblies and aggregates of these two EF-hand proteins in vitro.作者: 整潔漂亮 時間: 2025-4-1 00:25 作者: mercenary 時間: 2025-4-1 03:25
Preparation of Pure Populations of Amyloid β-Protein Oligomers of Defined Sizeiderable effort has been devoted to understanding the oligomerization process and to determining structure-activity relationships among the many types of oligomers that have been described. We discuss here a method for producing pure populations of amyloid β-protein (Aβ) of specific sizes using the 作者: 令人心醉 時間: 2025-4-1 06:17
