作者: OPINE 時(shí)間: 2025-3-21 22:55
Die technische Gewinnung von Stickstoff,fore, not only the development of GC systems, but also the concomitant development of suitable derivatives are important factors to accomplish satisfactory GC analysis. An enormous number of approaches for the preparation of volatile derivatives suitable for quantitative analysis of protein and nonprotein amino acids has been reported.作者: Compass 時(shí)間: 2025-3-22 01:09 作者: PIZZA 時(shí)間: 2025-3-22 04:44 作者: 縮減了 時(shí)間: 2025-3-22 10:05
Capillary Gas Chromatographic Analysis of Protein and Nonprotein Amino Acids in Biological Samples,fore, not only the development of GC systems, but also the concomitant development of suitable derivatives are important factors to accomplish satisfactory GC analysis. An enormous number of approaches for the preparation of volatile derivatives suitable for quantitative analysis of protein and nonprotein amino acids has been reported.作者: iodides 時(shí)間: 2025-3-22 13:41 作者: Phonophobia 時(shí)間: 2025-3-22 21:00
Total Plasma Homocysteine Analysis by HPLC with SBD-F Precolumn Derivatization,disease and stroke, such as plasma lipids, cigarette smoking, hypertension, obesity, and diabetes, only account for 50% of cardiovascular disease (.,.), one can understand the reason why homocysteine (Hcy) measurement is included in he list of tests for investigating the causes of atherosclerosis and thrombosis.作者: 飲料 時(shí)間: 2025-3-22 22:53 作者: Parley 時(shí)間: 2025-3-23 01:59
Anion Exchange Chromatography and Integrated Amperometric Detection of Amino Acids, detection measurement. Gradual or sudden fouling of the electrode surface by the products of electrode reaction and the corresponding decreased detection response is eliminated with properly designed PAD techniques. The first important application of PAD was for carbohydrates following their separation by anion exchange (.,.).作者: 圖畫(huà)文字 時(shí)間: 2025-3-23 08:34
Book 2000lection of these indispensable analytical techniques, both classic and cutting-edge, of high utility for answering specific biological questions. Common methods include those based on HPLC or gas chromatography separation and analysis after precolumn derivatization. New techniques based on capillary作者: 瘋狂 時(shí)間: 2025-3-23 12:16 作者: arousal 時(shí)間: 2025-3-23 17:43
Chemie des Cyans und der Cyanverbindungenisadvantages are that it requires a high-vacuum system and it takes a long time to remove the byproducts produced in the process of derivatization and the excess reagent in order to avoid interfering peaks.作者: Acetaldehyde 時(shí)間: 2025-3-23 21:19
https://doi.org/10.1007/978-3-662-33208-5teins with a long half-life like collagen and lens crystallin proteins (.,.). Therefore, determination of early glycation products and protein crosslinks produced because of glycation will be a significant aspect to investigate the extent of damage caused by NEG in vivo.作者: 有機(jī)體 時(shí)間: 2025-3-24 02:02 作者: Wordlist 時(shí)間: 2025-3-24 05:45 作者: 完成才會(huì)征服 時(shí)間: 2025-3-24 07:22
Determination of Early Glycation Products by Mass Spectrometry and Quantification of Glycation Mediteins with a long half-life like collagen and lens crystallin proteins (.,.). Therefore, determination of early glycation products and protein crosslinks produced because of glycation will be a significant aspect to investigate the extent of damage caused by NEG in vivo.作者: 侵害 時(shí)間: 2025-3-24 14:23
Purification of Proteins Using UltraMacro Spin Columns or ProSorb Sample Preparation Cartridges foronly used to recover proteins from buffers: a gel filtration method, Ultra microspin columns, and adsorption to a polyvinylidene difluoride (PVDF) membrane in the ProSorb sample preparation cartridge from Applied Biosystems. Both methods work well, with somewhat better recoveries for the gel filtrat作者: 猛擊 時(shí)間: 2025-3-24 17:26 作者: chapel 時(shí)間: 2025-3-24 21:49
Determination of Proteins, Phosphatidylethanolamine, and Phosphatidylserine in Lipid-Rich Materials amino acid derivatives are stable for months when stored dry at ?20°C, or for days in solution at ambient temperature (.,.). This approach can be used conveniently for analysis of lipid-rich material, where at least 300 samples can be analyzed with the same column, i.e., the column lifetime is comp作者: 小步舞 時(shí)間: 2025-3-24 23:32
Analysis of ,-Phosphoamino Acids in Biological Samples by Gas Chromatography with Flame Photometricorenylmethyl chloroformate (.,.), and CZE methods based on the UV derivatization with phenyl isothiocyanate or dabsyl chloride (.,.) were highly sensitive, but some of these methods lack specificity. Furthermore, many of these methods require lengthy separation time, and clean-up of the sample to re作者: Blatant 時(shí)間: 2025-3-25 04:44
Determination of Sulfur Amino Acids, Glutathione, and Related Aminothiols in Biological Samples by to the analysis of biological samples. As the HPLC methods, the ultraviolet (UV) (.–.), and the postcolumn UV derivatization with 4,4′-dithiopyridine (.) and 5,5-dithiobis (2-nitrobenzoic acid) (.), the precolumn fluorescence derivatization with 4-(aminosulfonyl)- or ammonium-7-fluoro-2,1,3-benzoxad作者: glucagon 時(shí)間: 2025-3-25 10:19
Capillary Electrophoretic Determination of 4-Hydroxyproline,ard the cathode with substantial velocity. This results in the flow of water termed electroosmosis. The flow proceeds from the anode to the cathode and serves as a pump. Because the electroosmotic water flow is much greater than the velocity of the analytes, all components are swept to the cathode.作者: Contort 時(shí)間: 2025-3-25 14:53
https://doi.org/10.1007/978-3-642-48550-3only used to recover proteins from buffers: a gel filtration method, Ultra microspin columns, and adsorption to a polyvinylidene difluoride (PVDF) membrane in the ProSorb sample preparation cartridge from Applied Biosystems. Both methods work well, with somewhat better recoveries for the gel filtrat作者: 騷動(dòng) 時(shí)間: 2025-3-25 17:09
https://doi.org/10.1007/978-3-663-05097-1n the method described here amino acids in standards, urine, or deproteinized serum/plasma are reacted with phenylisothiocyanate (PITC) in the presence of a coupling solvent. The phenylthiocyanate (PTC) derivatives are then applied to a reverse-phase (RP) HPLC column and separated using a gradient e作者: 平淡而無(wú)味 時(shí)間: 2025-3-25 21:32
Die Industrie der Steinkohlenveredelung amino acid derivatives are stable for months when stored dry at ?20°C, or for days in solution at ambient temperature (.,.). This approach can be used conveniently for analysis of lipid-rich material, where at least 300 samples can be analyzed with the same column, i.e., the column lifetime is comp作者: foodstuff 時(shí)間: 2025-3-26 00:17 作者: 千篇一律 時(shí)間: 2025-3-26 07:43 作者: Compatriot 時(shí)間: 2025-3-26 10:58
https://doi.org/10.1007/978-3-662-33208-5ard the cathode with substantial velocity. This results in the flow of water termed electroosmosis. The flow proceeds from the anode to the cathode and serves as a pump. Because the electroosmotic water flow is much greater than the velocity of the analytes, all components are swept to the cathode.作者: Valves 時(shí)間: 2025-3-26 15:45 作者: Coeval 時(shí)間: 2025-3-26 19:44
Book 2000imental results, and contains helpful notes on pitfalls to avoid, and variations that enable the methods to be used with different systems..Up-to-date and highly practical, Amino Acid Analysis Protocols offers analytical and clinical chemists, as well as a broad range of biological and biomedical in作者: Parabola 時(shí)間: 2025-3-26 22:33 作者: ANTIC 時(shí)間: 2025-3-27 04:02
Methods in Molecular Biologyhttp://image.papertrans.cn/a/image/154680.jpg作者: 火海 時(shí)間: 2025-3-27 07:23 作者: Counteract 時(shí)間: 2025-3-27 09:32 作者: Adjourn 時(shí)間: 2025-3-27 13:37 作者: Mettle 時(shí)間: 2025-3-27 20:28
https://doi.org/10.1007/978-3-642-48550-3Although lacking the speed and sensitivity of more widely heralded techniques such as mass spectrometry, amino acid analysis remains an indispensable tool in a complete biotechnology laboratory responsible for the analysis of protein pharmaceuticals.作者: adequate-intake 時(shí)間: 2025-3-28 01:09
Chemische Technologie in EinzeldarstellungenThere are several highly sensitive methods for identifying proteins: N-ter-minal sequencing, peptide mapping, and Western blotting, which are based on partial information about the structure of the protein. The amino acid composition of a protein is reflected in the primary structure of the whole protein, and this information is highly conserved.作者: Antecedent 時(shí)間: 2025-3-28 04:32
Amino Acid Analysis, Using Postcolumn Ninhydrin Detection, in a Biotechnology Laboratory,Although lacking the speed and sensitivity of more widely heralded techniques such as mass spectrometry, amino acid analysis remains an indispensable tool in a complete biotechnology laboratory responsible for the analysis of protein pharmaceuticals.作者: 冷漠 時(shí)間: 2025-3-28 07:48 作者: 豪華 時(shí)間: 2025-3-28 14:03
Amino Acid Analysis Protocols978-1-59259-047-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Spartan 時(shí)間: 2025-3-28 15:28
https://doi.org/10.1007/978-3-642-49786-5postcolumn derivatization techniques based on ion-exchange separation of the underivatized amino acids. The major reasons for this include greater sensitivity, faster analyses, and the ability to use less expensive, more flexible HPLC instrumentation rather than dedicated amino acid analyzers.作者: 變形 時(shí)間: 2025-3-28 20:25
https://doi.org/10.1007/978-3-642-49786-5erred method for quantitative amino acid analysis. The derivatization step introduces covalently bound chromophores necessary not only for interactions with the apolar stationary phase for high resolution, but also for photometric or fluorometric detection.作者: 過(guò)于平凡 時(shí)間: 2025-3-28 23:23 作者: 古文字學(xué) 時(shí)間: 2025-3-29 06:05
Wolfgang Jagodzinski,Markus Kleinwhich are shown in .. Amino acid analysis has an important role in the study of the composition of proteins, foods, and feedstuffs. Free amino acids are also determined in biological material, such as plasma and urine, and in fruit juice and wine. When it is performed on a pure protein, amino acid a作者: Catheter 時(shí)間: 2025-3-29 10:18
https://doi.org/10.1007/978-3-642-48550-3olysis, however, they are often not only at low concentration, but also in buffers, salts, and/or detergents. Sample contaminants contribute to background noise that hampers amino acid analysis, variably affecting recoveries of individual amino acids (.). The necessity to obtain samples in high conc作者: 終點(diǎn) 時(shí)間: 2025-3-29 14:33
https://doi.org/10.1007/978-3-642-49786-5postcolumn derivatization techniques based on ion-exchange separation of the underivatized amino acids. The major reasons for this include greater sensitivity, faster analyses, and the ability to use less expensive, more flexible HPLC instrumentation rather than dedicated amino acid analyzers.作者: enmesh 時(shí)間: 2025-3-29 17:32
https://doi.org/10.1007/978-3-642-49786-5erred method for quantitative amino acid analysis. The derivatization step introduces covalently bound chromophores necessary not only for interactions with the apolar stationary phase for high resolution, but also for photometric or fluorometric detection.作者: Muffle 時(shí)間: 2025-3-29 23:30 作者: FOR 時(shí)間: 2025-3-29 23:59
Waldemar Koch Dr.-Ing. Dr. Phil.nique was utilized with glassy carbon electrodes and in the mode of constant potential amperometry. The first widely used applications were for catecholamines and their derivatives (.). Today, most laboratories around the world still rely on electrochemical detection when it comes to analyzing catec作者: 富饒 時(shí)間: 2025-3-30 07:37 作者: generic 時(shí)間: 2025-3-30 10:49 作者: Melodrama 時(shí)間: 2025-3-30 16:09 作者: irritation 時(shí)間: 2025-3-30 18:47
https://doi.org/10.1007/978-3-663-05097-1ubular reabsorption (e.g., cystinuria) (..–.). In most patients with disorders in the former group, the abnormal amino acids are clearly increased in both plasma and urine, although the abnormality is usually more pronounced in urine. In disorders of renal tubular transport, only the urine amino aci作者: BRAND 時(shí)間: 2025-3-30 22:58 作者: Orthodontics 時(shí)間: 2025-3-31 04:49 作者: 休閑 時(shí)間: 2025-3-31 07:59 作者: 大門(mén)在匯總 時(shí)間: 2025-3-31 10:22
https://doi.org/10.1007/978-3-662-33208-5ssion of the basic theory of CE separations is beyond the scope of this presentation, but is well addressed in a number of recent texts (.–.). In principle, differential migration of analytes in a potential field is achieved as a result of individual differences in mass/charge ratios. In CE, substan作者: Conflagration 時(shí)間: 2025-3-31 17:19 作者: notice 時(shí)間: 2025-3-31 20:50 作者: JOT 時(shí)間: 2025-3-31 22:50
Amino Acid Analysis,which are shown in .. Amino acid analysis has an important role in the study of the composition of proteins, foods, and feedstuffs. Free amino acids are also determined in biological material, such as plasma and urine, and in fruit juice and wine. When it is performed on a pure protein, amino acid a作者: 乞討 時(shí)間: 2025-4-1 05:37 作者: Exhilarate 時(shí)間: 2025-4-1 08:46 作者: monochromatic 時(shí)間: 2025-4-1 10:47
Amino Acid Analysis by High-Performance Liquid Chromatography after Derivatization with 1-Fluoro-2,erred method for quantitative amino acid analysis. The derivatization step introduces covalently bound chromophores necessary not only for interactions with the apolar stationary phase for high resolution, but also for photometric or fluorometric detection.作者: 舊石器時(shí)代 時(shí)間: 2025-4-1 18:21
The Analysis of Amino Acids Using Precolumn Derivatization, HPLC, and Electrochemical Detection,on a derivatization reported by Jacobs (.) using o-phthalaldehyde in the presence of sulphite. The method was used by Pearson et al. (.), Smith and Sharp (.), and was further developed by Rowley et al. (.).
